Kmt2b (NM_029274) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2610014H22Rik; mKIAA0304; Mll2; Wbp7
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225396 representing NM_029274 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGGCGGCGGGCGGCGGCAGTTGCCCCGGGCCTGGCTCCGCACGGGGCCGCTTCCCGGGCCGGC CGCGGGGTTCCGGCGGGGGCGGGGGCCGCGGCGGCCGAGGCAACGGAGCCGAAAGAGTGCGGGTAGCCCT GCGGCGCGGTGGCGGCGCGGCGGGGCCGGGAGGAGCCGAGCCCGGGGAGGACACGGCCCTGCTCCGTTTG CTGGGTCTTCGCCGGGGCCTGCGCCGGCTCCGCCGCCTGTGGGCTGGTGCGCGAGTTCAGCGAGGCCGAG GCCGCGGCCGGGGACGGGGCTGGGGCCCGAACCGAGGCTGCATGCCGGAGGAAGAGAGCAGTGACGGGGA ATCCGAGGAGGAGGAGTTTCAGGGTTTTCATTCAGATGAAGATGTGGCCCCCAGTTCCCTGCGCTCTGCG CTTCGGTCCCAGCGAGGTCGAGCCCCTCGGGGTCGGGGCCGCAAGCATAAGACGACCCCCCTTCCTCCTC GCCTAGCAGATGTGACTCCTGTCCCCCCAAAGGCCCCTACTCGGAAACGGGGTGAGGAGGGCACAGAACG GATGGTGCAGGCACTGACTGAACTTCTCCGGCGGTCCCAAGCACCCCAACCCCCCCGGAGCCGGGCACGG GCACGTGAACCCTCTACTCCCCGACGGTCTCGGGGAAGGCCCCCAGGACGGCCAGCCGGTCCCTGCCGGA AAAAGCAGCAAGCAGTAGTGTTAGCAGAAGCGGCTGTGACAATCCCTAAACCCGAGCCTCCGCCCCCTGT GGTTCCAGTAAAGAACAAAGCTGGCAGTTGGAAATGCAAGGAGGGACCTGGTCCAGGACCTGGAACCCCC AAACGTGGAGGACAGCCTGGGAGAGGAGGCCGTGGAGGCAGGGGCAGAGGCCGAGGCGGACTTCCCCTTA TGATCAAGTTTGTTTCCAAGGCCAAGAAAGTGAAGATGGGACAATTGTCCCAGGAACTAGAATCAGGTCA GGGTCATGGTCAACGTGGGGAAAGCTGGCAGGATGCCCCCCAAAGAAAAGATGGAGATGAACCAGAAAGG GGCTCGTGTAGAAAGAAACAGGAGCAGAAACTGGAGGAGGAGGAGGAGGAGGAGGAGAAGGAGGGAGAAG AGAAGGAAGAAAAAGATGACAATGAGGATAACAACAAGCAAGAGGAAGAGGAGGAGACTGAGAGAGCTGT GGCTGAGGAAGAAGCGATGCTAGCCAAGGAGAAGGAAGAGGCAAAGCTTCCGTCACCACCCTTGACTCCT CCAGTCCCTTCACCACCTCCTCCTCTACCACCCCCTTCAACATCTCCTCCACCTCCAGCATCCCCTCTAC CACCCCCAGTTTCTCCTCCTCCCCCACTTTCCCCTCCCCCATATCCAGCCCCAGAGAAGCAGGAAGAGTC TCCTCCTCTTGTCCCAGCTACATGCTCTAGGAAGAGGGGCAGGCCTCCCCTGACTCCTAGCCAGCGGGCA GAGAGGGAAGCTGCTCGGTCAGGACCAGAGGGCACCCTTTCTCCCACTCCAAACCCTAGCACCACCACAG GAAGCCCTTTGGAAGACAGTCCTACTGTGGTCCCCAAAAGTACCACCTTTTTAAAGAATATACGGCAGTT TATTATGCCTGTAGTGAGTGCTCGCTCCTCCCGTGTTATCAAGACACCCCGGCGATTTATGGATGAAGAT CCTCCCAAACCTCCAAAGGTGGAGGCTTCTATTGTTCGACCTCCTGTTGCAACCTCCCCACCTGCTCCCC AGGAACCAGTACCAGTCTCCTCTCCACCTCGTGTCCCAACTCCCCCATCTACCCCAGTCCCACTCCCTGA GAAGAGGCGGTCTATCCTAAGGGAACCCACATTTCGCTGGACCTCACTAACCCGGGAGCTGCCGCCACCT CCACCTGCTCCTCCACCAGCCCCATCTCCACCACCTGCCCCTGCCACTCCCTCCCGGAGGCCCTTGCTCC TTCGGGCCCCTCAGTTTACCCCAAGTGAAGCCCACCTGAAGATCTATGAATCGGTGCTTACCCCTCCTCC TCTTGGGGCTCTTGAAACCCCTGAGCCAGAGCTGCCTCCTGCTGATGACTCTCCAGCAGAGCCTGAGCCT AGGGCAGTGGGGCGGACCAATCACCTCAGCTTGCCTCGCTTTGTCCCTGTGGTCACCAGTCCTGTTAAGG TTGAGGTGCCCCCCCATGGAGCTCCAGCTCTGAGTGAAGGCCAGCAGCTTCAGCTCCAGCAGCCCCCGCA GGCCTTGCAGACCCAGCTCCTACCCCAGGCACTACCACCACAGCAACCACAAGCGCAGCCACCTCCATCA CCACAACACACACCACCACTGGAAAAGGCCCGTGTTGCAAGTCTGGGTTCCCTGCCACTATCTGGGGTGG AGGAGAAAATGTTTAGCCTTCTTAAGAGAGCTAAGGTGCAGTTATTCAAGATCGATCAACAGCAACAGCA GAAAGTGGCGGCTTCAATGCCGCTAAGCCCTGCAGTACAAACCGAGGAGGCTGTGGGGACTGTCAAACAG ACCCCGGACAGAGGCTGTGTCAGGTCTGAAGATGAATCCATGGAAGCTAAGAGAGATCGAGCCTCGGGCC CTGAGTCACCTTTACAAGGCCCCCGAATCAAACATGTCTGCCGTCACGCTGCTGTGGCCTTGGGTCAGGC TCGGGCCATGGTGCCTGAAGATGTACCCCGCCTCAGTGCCCTCCCTCTCCGGGATCGGCAGGACCTCGCC ACTGAGGATACGTCATCAGCATCAGAGACTGAGAGTGTCCCTTCCCGATCCCAGCGGGAAAAGGTGGAAT CTGCAGGGCCTGGGGGAGACTCGGAGCCTACAGGGTCTACAGGGGCCCTGGCCCACACACCACGGCGGTC ACTGCCATCCCACCATGGCAAGAAGATGAGAATGGCTCGTTGTGGGCACTGTCGGGGTTGCTTGCGTGTG CAGGACTGTGGCTCTTGTGTCAACTGCTTGGATAAACCTAAATTTGGGGGTCCCAACACTAAGAAGCAGT GCTGTGTATACCGGAAGTGTGACAAGATAGAGGCTCGGAAGATGGAGCGGCTGGCAAAAAAAGGCCGGAC GATAGTGAAGACGCTGTTGCCCTGGGATTCCGATGAATCTCCTGAGGCCTCCCCTGGTCCTCCAGGCCCA CGCCGGGGGGCGGGAGCTGGGGGGTCCCGGGAGGAGGTGGGGGCCACTCCCGGGCCCGAGGAGCAGGACT CCCTCCTACTGCAGCGCAAGTCAGCCCGACGCTGCGTCAAACAGCGGCCCTCCTATGATGTCTTCGAGGA CTCAGATGACTCAGAGCCCGGGGGTCCTCCTGCTCCAAGACGTCGCACCCCCAGAGAACATGAGCTGCCA GTACTAGAACCTGAGGAGCAGAGCCGGCCCCGCAAACCCACCCTGCAGCCTGTGTTGCAGCTCAAGGCCC GAAGGCGCCTGGACAAGGATGCTTTAGCCCCTGGCCCCTTTGCCTCTTTTCCCAATGGCTGGACTGGAAA GCAGAAGTCTCCTGACGGTGTGCACCGGGTCCGAGTAGATTTTAAGGAGGATTGCGACCTAGAGAATGTG TGGCTTATGGGTGGTCTCAGTGTGCTGACTTCTGTGCCAGGGGGGCCACCGATGGTGTGTCTGCTGTGTG CTAGCAAAGGCCTCCATGAGCTGGTTTTTTGCCAAGTCTGCTGCGATCCTTTCCACCCCTTCTGTCTGGA GGAGGCTGAGCGGCCCTCACCTCAGCATCGTGACACCTGGTGCTGCAGGCGCTGCAAGTTCTGCCATGTT TGTGGGCGCAAAGGCCGAGGGTCCAAGCACCTCCTGGAATGTGAACGCTGTCGTCATGCTTACCACCCAG CCTGCCTGGGACCCAGCTACCCAACCCGGGCCACGCGTAGACGGCGCCACTGGATCTGTTCAGCCTGTGT GCGCTGTAAGAGCTGTGGGGCAACTCCAGGCAAGAACTGGGATGTCGAGTGGTCTGGAGATTACAGCCTC TGCCCCAGGTGCACCGAGCTCTATGAAAAAGGGAACTACTGCCCAATTTGCACACGCTGCTATGAAGACA ATGACTATGAGAGCAAGATGATGCAGTGTGCACAGTGTGACCACTGGGTGCATGCCAAGTGTGAGGGACT CTCAGATGAAGACTACGAGATCCTTTCTGGACTGCCAGACTCAGTGTTGTACACGTGTGGGCCATGTGCT GGGGCGACACAGCCCCGCTGGCGAGAGGCCCTGAGTGGGGCCCTGCAGGGAGGCCTGCGTCAGGTGCTCC AGGGCCTGTTAAGCTCAAAGGTGGCAGGCCCATTGCTGCTGTGCACCCAGTGTGGGCAGGATGGGAAGCA GCTCCACCCAGGACCCTGTGATTTACAAGCTGTGGGAAAGCGCTTTGAGGAAGGTCTCTATAAGTCTGTG CACAGCTTCATGGAAGACGTAGTGGCCATCTTGATGAGGCATTCAGAAGAAGGAGAGACCCCAGAGCGCC GGGCTGGAAGCCAGATGAAAGGACTCCTACTGAAGCTGCTAGAGTCTGCGTTCTGCTGGTTTGACGCCCA TGATCCTAAGTACTGGCGACGGAGTACACGGCTGCCAAACGGAGTGCTCCCCAATGCTGTGTTGCCTCCA TCCTTGGACCATGTCTATGCTCAGTGGAGACAACAGGAATCAGAGACCCCAGAATCGGGGCAGCCTCCAG GGGATCCTTCAGCAGCTTTCCAGAGCAAGGACCCAGCTGCTTTCTCACACCTGGATGACCCTCGTCAGTG TGCACTCTGTCTCAAGTATGGGGATGCTGACTCTAAGGAGGCGGGGCGGCTCCTGTACATTGGGCAGAAT GAGTGGACACATGTCAATTGTGCCATTTGGTCAGCTGAAGTGTTTGAGGAGAATGATGGCTCCCTCAAGA ACGTCCATGCTGCTGTTGCACGTGGAAGGCAGATGCGCTGTGAGCTCTGCCTCAAGCCTGGAGCCACAGT TGGCTGCTGCCTGTCCTCATGTCTCAGCAACTTCCACTTCATGTGTGCCCGGGCCAGCTACTGCATCTTC CAGGATGACAAGAAGGTTTTCTGCCAGAAACACACAGACCTCCTAGATGGCAAGGAGATCGTGACCCCTG ATGGTTTTGATGTTCTTCGGCGAGTCTATGTGGACTTCGAGGGCATCAACTTCAAGCGAAAGTTCTTGAC TGGACTTGAACCAGATGTCATCAATGTACTCATTGGCTCCATCAGAATCAACTCCCTGGGCACCCTATCT GATCTCTCAGACTGTGAGGGTCGACTCTTCCCCATTGGCTACCAATGCTCCCGCCTGTATTGGAGCACAG TGGATGCTCGGAGGCGCTGCTGGTATCGGTGCCGAATTCTAGAGTATCGCCCTTGGGGGCCAAGGGAGGA GCCAGTGCACCTAGAAGCAGCAGAGGAGAACCAGACCATTGTGCATAGCCCAACCCCTTCCTCAGATACA GATTCTCTGATCCCTGGAGATCCTGTACACCACTCCCCCATTCAGAATCTGGATCCCCCGCTTCGGACAG ATTCAAGCAATGGCCCTCCTCCCACCCCTCGCTCCTTCTCAGGGGCTCGAATCAAAGTGCCCAACTACTC ACCGTCTCGGAGGCCCTTAGGGGGAGTCTCTTTTGGTCCTCTGCCCTCTCCAGGAAGTCCATCTTCCCTG ACCCACCACATCCCTACTGTGGGAGACTCGGACTTTCCAGCTCCCCCAAGGCGATCTCGGCGTCCTAGCC CCCTGGCCACCAGGCCACCACCATCCCGGAGGACCTCCTCTCCTCTCAGAACTTCCCCTCAGCTCAGGGT GCCCCTGTCTACCTCAGTCACAGCCCTCACACCTACCTCAGGGGAGCTGGCTCCCCCTGACCTGGCCCCA TCTCCCCTTCCACCGTCTGAAGACCTGGGCCCAGACTTTGAAGACATGGAGGTGGTGTCAGGACTAAGTG CTGCTGACCTGGACTTTGCAGCCAGCCTGCTGGGGACTGAGCCTTTCCAGGAAGAGATTGTGGCTGCTGG GGCAGTGGGGAGCAGCCAGGGAGGTCCAGGGGACAGCTCAGAGGAGGAGGCCAGCCCTACCACTCACTAT GTCCACTTCCCTGTGACCGTGGTGTCTGGCCCTGCCCTGGCCCCCAGTTCCCTTGCTGGAGCTCCCCGAA TCGAGCAGCTGGACGGAGTGGATGATGGCACAGACAGCGAGGCTGAGGCTGTCCAACAACCCCGGGGACA GGGGACTCCTCCTTCAGGACCAGGAGTGGGCCGAGGAGGGGTCCTTGGAGCAGCAGGCGACAGAGCCCAA CCTCCTGAAGACCTGCCTTCAGAAATTGTGGATTTCGTGTTGAAGAACCTAGGGGGTCCTGGGGAGGGGG CTGCTGGCCCCAGAGAGGACTCTCTTCCTTCAGCACCTCCCTTGGCTAATGGCAGCCAACCCCCACAAAG CCTGTCCACTAGCCCAGCTGACCCCACCCGAACATTTGCTTGGCTCCCTGGAGCTCCAGGGGTCCGGGTA TTGAGTCTGGGTCCTGCTCCAGAACCCCCCAAACCTGCCACATCCAAGATCATCCTTGTCAACAAGCTGG GGCAAGTATTTGTGAAGATGGCAGGGGAGGGTGAGCCTGTAGCACCCCCAGTAAAGCAGCCCCCTCTACC CCCCATCATTCCCCCAACAGCTCCCACCTCCTGGACTCTGCCCCCAGGACCCCTGCTCAGTGTGTTACCA GTGGTAGGGGTAGGAGTGGTCCGGCCTGCCCCACCCCCACCTCCACCCCCACTAACACTGGTGTTTAGCA GTGGGCCCCCCAGCCCACCACGCCAGGCCATTCGTGTCAAGAGGGTATCCACCTTCTCTGGCCGATCCCC TCCAGTTCCTCCCCCAAACAAAACTCCCCGGCTGGATGAAGATGGAGAGTCCTTGGAGGATGCCCATCAT GTGCCAGGGATCAGTGGCAGTGGGTTCAGTCGTGTTCGCATGAAAACGCCCACAGTCCGTGGAGTTCTGG ACCTGAACAATCCTGGGGAGCAGCCTGAGGAGGAAAGCCCTGGGCGCCCCCAGGACCGGTGTCCTTTGCT GCCACTTGCAGAAGCTCCTTCCCAGGCCCTCGATGGTTCCTCAGACCTACTGTTTGAGTCCCAGTGGCAT CACTATTCAGCAGGCGAGGCCTCAAGCTCTGAGGAAGAGCCTCCATCTCCAGAGGACAAAGAAAACCAGG TCCCCAAACGGGTTGGTCCACACCTGCGCTTTGAGATCAGCAGTGATGATGGCTTCAGTGTGGAGGCAGA GAGCTTGGAGGTGGCATGGAGAACTCTGATTGAGAAAGTGCAAGAAGCACGAGGGCATGCACGGCTCAGG CATCTCTCCTTTAGTGGAATGAGTGGAGCGCGGCTCCTGGGCATCCACCACGATGCTGTCATCTTCCTGG CAGAGCAGCTCCCTGGGGCCCAGCGCTGCCAGCATTATAAGTTCCGCTACCACCAGCAGGGAGAGGGCCA GGAAGAGCCACCTCTGAATCCCCATGGGGCTGCTCGTGCTGAGGTCTATCTCCGGAAGTGTACCTTTGAC ATGTTCAACTTCCTGGCCTCCCAGCACCGTGTGCTCCCCGAGGGAGCCACCTGTGATGAAGAAGAGGATG AGGTGCAGCTTCGGTCCACCAGACGTGCCACCAGCCTGGAGCTTCCTATGGCCATGCGCTTTCGTCATCT CAAAAAGACATCCAAAGAGGCTGTGGGAGTCTACAGATCAGCTATCCATGGACGAGGTCTGTTTTGTAAA CGCAACATCGATGCGGGAGAGATGGTCATTGAGTACTCTGGCATTGTCATTCGCTCTGTGCTGACTGACA AGCGAGAGAAGTTCTATGATGGGAAGGGTATTGGGTGCTACATGTTCCGCATGGATGACTTTGATGTGGT GGACGCCACCATGCACGGCAATGCTGCCCGATTCATCAACCACTCATGTGAGCCCAACTGCTTCTCTCGT GTCATCCATGTGGAAGGCCAGAAACACATTGTCATCTTTGCCTTGCGCCGCATCCTGCGAGGTGAGGAGC TCACCTATGACTACAAGTTTCCTATCGAGGATGCCAGCAACAAGCTGCCCTGCAACTGTGGCGCCAAGCG CTGCCGTCGCTTTCTTAACTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_029274
Insert Size	8142 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_029274.2, NP_083550.2
RefSeq Size	8457 bp
RefSeq ORF	8142 bp
Locus ID	75410
UniProt ID	O08550
Gene Summary	Histone methyltransferase. Methylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in beta-globin locus transcription regulation by being recruited by NFE2 (By similarity). Plays an important role in controlling bulk H3K4me during oocyte growth and preimplantation development. Required during the transcriptionally active period of oocyte growth for the establishment and/or maintenance of bulk H3K4 trimethylation (H3K4me3), global transcriptional silencing that preceeds resumption of meiosis, oocyte survival and normal zygotic genome activation.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.