Cep290 (NM_146009) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	b2b1454Clo; b2b1752Clo; BC004690; Nphp6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225335 representing NM_146009 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCACCTAATATAAAGTGGAAAGAATTAATCAAAGTTGATCCAGATGACCTGCCACGGCAAGAAGAGT TAGCAGATAAATTATTGATATCTTTATCCAAGGTGGAAGTAAATGAACTAAAAAATGAAGACCAAGAAAA CATGATACATCTATTCAGAATTACCCAGTCTCTAATGAAGATGAAAGCCCAGGAAGTAGAGCTCGCTTTG GAAGAAGTTGAAAAGGCTGGAGAAGAACAAGCAAAATTTGAAAATCAATTAAAGACAAAAGTAATGAAAC TGGAAAATGAACTGGAGATGGCTCAGCAGTCTGCAGGGGGACGTGACACTCGGTTTTTACGTGACGAAAT TCGCCAACTTGAGAAGCAGCTGGAACAAAAAGATAGAGAATTAGAGGATATGGAAAAAGAATTGGATAAA GAAAAGAAGGTTAATGAACAATTGGCTCTTCGAAATGAGGAGGCAGAAAATGAAAACAGCAAATTAAGAA GAGAGAACAAACGTCTAAAGAAAAAGAATGAGCAGCTTCGGCAGGACATTATTGACTACCAGAAACAAAT AGATTCACAGAAAGAATCACTTCTGTCAAGGAGAGGAGAAGACAGTGACTACCGATCACAGTTGTCTAAA AAGAACTATGAACTTGTTCAATATCTGGACGAAATACAGACCTTAACAGAAGCTAATGAGAAGATTGAAG TTCAGAACCAAGAAATGAGGAAAAACCTGGAAGAGTCTGTGCAGGAGATGGAGAAGATGACTGATGAGTA CAACAGGATGAAAGCGCTTGTGCATCAGTCGGATGCTGTCATGGACCAGATCAAGAAGGAGAATGAGCAC TATCGCCTGCAAGTTCGAGAGCTCACGGATCTTCTGAAGGCGAAGGATGAAGAGGACGATCCAGTCATGA TGGCTGTTAACGCAAAAGTGGAAGAGTGGAAGTTAATTTTGTCTTCTAAAGATGATGAAATCATTGAATA TCAGCAAATGCTACAGAGTCTGAGAGGGAAACTTAAAAATGCCCAACTTGATGCAGACAAAAGTAACATC ATGGCTCTGAAACAGGGTATCCAGGAGCGAGACAGTCAAATTAAGATGCTTACTGAGCAAGTAGAACAGT ATACGAAAGAAATGGAAAAAAATACGTTTATTATTGAAGATTTGAAAAATGAACTCCAAAAAGACAAAGG TACTTCAAACTTTTATCAGCAGACTCATTATATGAAAATTCACTCAAAAGTACAAATTTTAGAAGAGAAA ACAAAAGAGGCCGAGAGAATAGCTGAGCTGGCTGAGGCTGATGCCAGGGAGAAGGACAAAGAATTGGTTG AGGCTCTGAAGAGATTAAAAGATTATGAATCTGGAGTATATGGCTTAGAAGATGCTGTTATCGAAATAAA GAATTGTAAAGCCCAAATTAAAATAAGAGATGGAGAGATGGAAGTGTTGACCAAGGAGATCAATAAGCTT GAGATGAAGATCAATGACATCCTTGATGAAAACGAAGCCCTTAGAGAGCGGGCTGGCCTTGAACCCAAGA CAATGATTGATTTGACTGAATTTAGAAACAGCAAACGGTTAAAGCAGCAACAGTACAGAGCTGAGAACCA GGTTCTTTTGAAAGAGATCGAAAGTCTAGAGGAGGAGCGTCTTGACTTGAAAAGAAAGATTCGTCAAATG GCTCAAGAAAGAGGCAAAAGGAACGCAGCCTCAGGATTAACCATTGACGACTTGAACTTATCTGAAACCT TTTCTCATGAAAATAAAATAGAAGGAAGAAAACTAAATTTTATGAGCCTCAATAATATGAATGAAACACA ATCAAAGAATGAGTTTCTTTCAAGAGAATTGGCTGAAAAGGAAAAAGATTTAGAAAGAAGTAGGACAGTA ATTGCTAAATTCCAGAGTAAACTAAAAGAATTAGTCGAAGAAAATAAGCAACTTGAAGAAGGTATGAAAG AAATTTTGCAAGCTATTAAGGACATGCCGAAGGATTCTGATGTGAAAGGAGGTGAAACATCTTTAATCAT CCCGAGTCTTGAAAGACTGGTTAACGCTATGGAATCAAAGAATGCAGAAGGAATCTTCGATGCCAGCTTG CATCTAAAAGCCCAAGTTGACCAACTTACAGGAAGGAATGAAGAATTAAGGCAAGAACTCAGGCAATCTC GGAAAGAGGCTGTAAATTATTCACAGCAGTTGGTAAAAGCAAACTTAAAGATTGACCACCTTGAGAAGGA AACTGACCTTCTACGTCAGTCTGCAGGCTCCAATGTAGTATACAAAGGCATAGACTTACCCGATGGGATA GCACCATCCAGTGCCTATATCATCAATTCTCAGAATGAATACTTAATACATCTTTTGCAGGAACTAGACA ATAAAGAAAAGAAATTAAAACATTTAGAAGATTCCCTTGAAGATTATAACAGAAAGTTTGCAGTGATTCG TCATCAACAAAGTTTATTATATAAAGAATACCTAAGTGAAAAGGATATTTGGAAAACAGACTCTGAGATG ATAAGAGAGGAGAAGAGAAAACTGGAAGATCAAGCTGAGCAGGATGCTGTGAAAGTAAAAGAGTACAACA ATTTGCTCAGTGCTCTTCAGATGGATTCGAACGAGATGAAGAAAATGCTCTCAGAAAACAGTAGGAAAAT CACCGTTCTGCAAGTGAACGAGAAGTCCCTCATCCGGCAGTACACCACCTTAGTGGAGATGGAGCGGCAC CTGAGAAAGGAAAACGGGAAACACAGGAATGACGTCATAGCCATGGAAGCCGAAGTCACTGAAAAACTTG GAAGTTTGCAAAGATTCAAGGAAATGGCCATCTTCAAGATTGCAGCTCTTCAGAAGGTTATAGATAATAG TGTTTCTTTGTCTGAATTAGAACTAGCCAATAAACAGTACAATGAGCTGACTACTAAGTACAGGGACATC TTGCAGAAAGATAATATGCTTGTTCAAAGAACAAGTAACTTAGAACACCTGGAGTGTGAGAATGCATCGC TAAAAGAACAGATGGAGGCTATCAGTAAAGAGCTGGAGATTACAAAGGAAAAACTCCATACCATTGAACA GGCCTGGGAACAAGAAACGAAGTTAGGAAATGACTCAAACATGGATAAGGCTAAGAAATCAATGACAAAC AGTGACATTGTTTCTATTTCAAAGAAAATCACTGTGTTGGAGATGAAGGAATTAAATGAAAGGCAGAGGG CTGAACACTGTCAGAAAATGTATGAGCACTTAAGGACTTCATTAAAGCAAATGGAAGAACGTAATTTTGA ATTGGAAACCAAGTTCACTGAGCTTACTAAAATCAACCTGGATGCACAAAAGGTGGAGCAGATGTTGAGA GACGAATTAGCTGATAGTGTGACCAAGGCAGTAAGTGATGCTGACCGACAGCGGATTCTAGAACTAGAGA AGAGTGAAGTGGAGCTCAAAGTTGAAGTGTCCAAGCTGAGAGAGATTTCAGATATTGCCAAAAGACAAGT TGATTTTTTGAATTCGCAACAACAGTCCAGGGAAAAGGAAGTGGAATCCCTCAGAACGCAGCTGCTGGAC TTCCAGGCACAATCTGACGAAAAGGCTCTAATTGCCAAATTGCACCAACATGTTGTCTCTCTTCAAATTA GTGAGGCCACTGCCCTCGGTAAGTTAGAGTCAGTTACGTCCAAACTCCAGAAGATGGAAGCCTACAATTT GCGCTTAGAACAGAAACTGGATGAAAAAGAGCAGGCGCTCTACTATGCTCGTTTGGAAGGTAGAAACAGA GCAAAACACCTGCGCCAAACCATTCAGTCGCTTCGAAGACAGTTCAGTGGAGCTCTACCCTTAGCACAGC AGGAAAAGTTCTCCAAAACGATGATTCAGTTGCAAAATGACAAACTTAAGATAATGCAAGAAATGAAGAA TTCGCAACAGGAACACAGAAATATGGAAAACAAAACACTGGAGTTGGAATTAAAATTAAAAGGCTTAGAA GAATTGATCAGTACTTTAAAGGATGCCAGGGGAGCCCAGAAGGTAATCAATTGGCATGTGAAAATAGAAG AACTTCGCCTCCAAGAACTTAAGCTAAATAGAGAACTAGTCAAGGGTAAAGAAGAAATCAAATATTTGAA TAATATCATCTCTGAATATGAGCATACAATCAACAGTCTAGAGGAAGAAATTGTTCAGCAAAGCAAGTTC CATGAAGAAAGACAGATGGCTTGGGATCAAAGAGAAGTTGAGCTGGAACGCCAGTTAGACATTTTTGATC ATCAGCAAAATGAAATACTCAGTGCAGCACAAAAGTTTGAAGACTCTACAGGATCAATGCCAGACCCCAG CTTGCCTCTTCCAAACCAACTTGAAATTGCTCTAAGAAAAATTAAGGAGAATATTCAAGTAATTCTTAAA ACACAAGCAACTTGCAAGTCACTAGAAGAGAAACTAAAAGAAAAAGAATCTGCTTTACGGTTGGCAGAGC AAAATATTCTGTCAAGAGACAAAGTAATCAATGAACTGAGGCTTCGATTGCCTGCCACGGCTGATCGAGA AAAACTTATAGCTGAGCTAGAAAGAAAAGAGCTGGAGCCGAAATCTCATCACACAATGAAAATTGCCCAC CAAACTATTGCCAACATGCAGGCAAGGTTAAATCACAAGGAAGAAGTATTGAAGAAATACCAGCACCTTC TGGAGAAGGCCAGAGAGGAGCAAAGAGAAATTGTTAAGAAGCATGAGGAAGACCTTCATGTTCTTCATCA CAAATTAGAACAACAGGCCGATAATTCACTCAATAAATTCAGACAGACAGCTCAGGATTTACTTAAGCAG TCTCCTGCTCCAGTTCCCACCAACAAACATTTCATTCGTCTGGCCGAGATGGAGCAGACAGTAGCAGAAC AAGATGACTCTCTGTCCTCACTTTTGACCAAACTAAAGAAAGTATCAAAAGATTTGGAAAAACAAAAAGA AATCACTGAGTTAAAAGTCAGAGAGTTTGAAAATACCAAACTACGGCTCCAAGAAACTCATGCCAGTGAG GTAAAGAAAGTGAAAGCAGAGGTAGAGGACTTAAGGCATGCTCTAGCCCAAGCACACAAGGACTCCCAGA GTTTAAAGTCTGAACTCCAGGCTCAGAAAGAAGCAAACTCCAGAGCTCCAACAACCACAATGAGGAATCT TGTAGACAGGCTAAAGAGCCAACTAGCCTTGAAAGAGAAGCAACAAAAGGCACTTAGTCGAGCCCTGTTG GAACTTCGGTCGGAAATGACAGCAGCAGCTGAGGAACGTATAATCGCTGTAACTTCTCAAAAAGAGGCAA ATCTCAATGTTCAACAAGTTGTTGAGCGCCATACTAGAGAGCTAAAGTCACAAATTGAAGATTTAAATGA AAATCTTTTAAAATTGAAAGAAGCTCTTAAAACAAGTAAGAACAAAGAAAATTCACTAGCTGATGATTTA AATGAATTAAATAATGAACTGCAAAAAAAGCAAAAAGCTTATAATAAAATCCTTAGAGAGAAAGATGGAA TTGATCAAGAAAATGATGAACTGAGAAGACAGATTAAAAGACTGTCCAGTGGACTGCAGAGCAAAACTTT GATAGATAACAAGCAAAGTTTAATCGATGAACTTCAAAAGAAAGTTAAAAAACTTGAAAGCCAACTGGAA AGAAAGGTGGATGACGTAGACATAAAGCCGGTGAAGGAAAAGAGTAGTAAAGAAGAATTAATTAGGTGGG AAGAAGGTAAGAAATGGCAAACCAAAGTAGAGGGACTACGAAACAGACTAAAGGAGAAGGAAGGAGAAGC CCACGGCCTGGCAAAGCAGCTGAATACCTTAAAGGAACTTTTTGCCAAAGCTGATAAAGAGAAACTTACT TTGCAGAAGAAACTGAAAACAACAGGAATGACTGTTGACCAGGTTTTAGGAGTGCGAGCTTTGGAATCTG AAAAAGAGTTGGAAGAGCTAAAAAAGAAAAATCTGGACCTAGAAAATGACATATTATACATGAGGACCCA GCAGGCTCTTCCACGAGATTCTGTTGTGGAAGACTTACATTTACAAAATAAATACCTTCAAGAAAAACTT CATACTTTAGAAAAAAAACTTTCAAAGGAGAAATATTCTCAGTCTTTGACTTCAGAAATAGAGTCAGATG ATCACTGTCAAAAAGAACAAGAACTTCAGAAGGAAAATTTGAAGTTGTCATCTGAAAACATCGAGCTGAA ATTTCAACTTGAACAAGCAAATAAAGATTTGCCAAGACTAAAGAATCAAGTGAAAGATTTGAAGGAAATG TGTGAATTTCTTAAGAAAGGAAAACTGGAACTTGAGCGGAAGCTTGGTCAGGTCAGAGGGGCTGGTAGAA GTGGGAAGACAATCCCAGAACTAGAAAAAACCATTGGGTTAATGAAGAAAGTAGTTGAAAAAGTCCAAAG AGAAAATGAACAATTGAAAAAGGCATCAGGAATACTGACTAGTGAAAAAATGGCTACTATTGAGGAAGAA AATAGAAACTTAAAGGCTGAACTAGAAAAGCTTAAAGCTCACTTTGGACGTCAGTTGAGTATGCAGTTTG AATCTAAGAACAAAGGTACTGAGAAAATTGTTGCCGAAAATGAACGGCTTCGGAAAGAACTTAAGAAAGA AATAGAAGCCTCTGAGAAACTGCGGATAGCTAAGAACAACTTAGAGCTGGTGAACGACAAGATGGCAGCT CAACTCGAAGAAACTGGGAAGAGACTACAGTTTGCAGAAAGTAGAGCCCCACAGCTGGAAGGTGCTGACA GCAAGAGCTGGAAGTCAATTGTGGTCTCAAGAGTGTATGAGACCAAGATGAAAGAGCTTGAAAGTGACAT TGCCAAAAAGAATCAAAGTATCACTGACCTTAAACAGCTTGTAAGAGAAGCAACAGAGAGAGAACAGAAA GCTAAGAAATACACTGAAGACCTTGAACAACAGATTGAGATCCTCAAAAATGTTCCTGAAGGTGCCGAGA CAGAGCAAGAGCTTATACGGGAACTCCAGCTTCTTAGATTAGCCAATAATCAGATGGATAAAGAAAGGGC AGAATTAATCCATCAGATAGAAATTAACAAGGACCAAACCAGAGCTGACAGTAGCATACCTGATTCTGAT CAACTAAAGGAAAAGATAAATGACCTGGAGACACAACTCAGAAAGTTGGAGCTAGAAAAGCAACATTCGA AGGAGGAAGTTAAAAAGCTGAAAAAAGAACTGGAAAATTTTGATCCTTCATTTTTTGAAGAAATTGAAGA CCTGAAGTATAATTATAAGGAAGAAGTGAAAAAGAATATCCTATTAGAAGAGAAGCTAAAAAAACTGTCG GAACAGTTTGGATTTGAACTGCCTAGTCCTCTTGCTGCTTCTGAACACTCGGAAGATGGAGAAAGTCCTC ATAGTTTCCCTATTTATTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_146009
Insert Size	7440 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_146009.2, NP_666121.2
RefSeq Size	8006 bp
RefSeq ORF	7440 bp
Locus ID	216274
UniProt ID	Q6A078
Gene Summary	Involved in early and late steps in cilia formation (PubMed:21565611). Its association with CCP110 is required for inhibition of primary cilia formation by CCP110 (By similarity). May play a role in early ciliogenesis in the disappearance of centriolar satellites and in the transition of primary ciliar vesicles (PCVs) to capped ciliary vesicles (CCVs). Required for the centrosomal recruitment of RAB8A and for the targeting of centriole satellite proteins to centrosomes such as of PCM1 (By similarity). Required for the correct localization of ciliary and phototransduction proteins in retinal photoreceptor cells; may play a role in ciliary transport processes (PubMed:16632484). Required for efficient recruitment of RAB8A to primary cilium (By similarity). In the ciliary transition zone is part of the tectonic-like complex (also named B9 complex) which is required for tissue-specific ciliogenesis and may regulate ciliary membrane composition (PubMed:21725307). Involved in regulation of the BBSome complex integrity, specifically for presence of BBS2, BBS5 and BBS8/TTC8 in the complex, and in ciliary targeting of selected BBSome cargos. May play a role in controlling entry of the BBSome complex to cilia possibly implicating IQCB1/NPHP5 (By similarity). Activates ATF4-mediated transcription (By similarity).[UniProtKB/Swiss-Prot Function]

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