Cep290 (NM_146009) Mouse Untagged Clone

CAT#: MC225335

Cep290 (untagged) - Mouse centrosomal protein 290 (Cep290), (10ug)



  "NM_146009" in other vectors (2)

CNY 39,710.00


货期*
12周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms b2b1454Clo; b2b1752Clo; BC004690; Nphp6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC225335 representing NM_146009
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCACCTAATATAAAGTGGAAAGAATTAATCAAAGTTGATCCAGATGACCTGCCACGGCAAGAAGAGT
TAGCAGATAAATTATTGATATCTTTATCCAAGGTGGAAGTAAATGAACTAAAAAATGAAGACCAAGAAAA
CATGATACATCTATTCAGAATTACCCAGTCTCTAATGAAGATGAAAGCCCAGGAAGTAGAGCTCGCTTTG
GAAGAAGTTGAAAAGGCTGGAGAAGAACAAGCAAAATTTGAAAATCAATTAAAGACAAAAGTAATGAAAC
TGGAAAATGAACTGGAGATGGCTCAGCAGTCTGCAGGGGGACGTGACACTCGGTTTTTACGTGACGAAAT
TCGCCAACTTGAGAAGCAGCTGGAACAAAAAGATAGAGAATTAGAGGATATGGAAAAAGAATTGGATAAA
GAAAAGAAGGTTAATGAACAATTGGCTCTTCGAAATGAGGAGGCAGAAAATGAAAACAGCAAATTAAGAA
GAGAGAACAAACGTCTAAAGAAAAAGAATGAGCAGCTTCGGCAGGACATTATTGACTACCAGAAACAAAT
AGATTCACAGAAAGAATCACTTCTGTCAAGGAGAGGAGAAGACAGTGACTACCGATCACAGTTGTCTAAA
AAGAACTATGAACTTGTTCAATATCTGGACGAAATACAGACCTTAACAGAAGCTAATGAGAAGATTGAAG
TTCAGAACCAAGAAATGAGGAAAAACCTGGAAGAGTCTGTGCAGGAGATGGAGAAGATGACTGATGAGTA
CAACAGGATGAAAGCGCTTGTGCATCAGTCGGATGCTGTCATGGACCAGATCAAGAAGGAGAATGAGCAC
TATCGCCTGCAAGTTCGAGAGCTCACGGATCTTCTGAAGGCGAAGGATGAAGAGGACGATCCAGTCATGA
TGGCTGTTAACGCAAAAGTGGAAGAGTGGAAGTTAATTTTGTCTTCTAAAGATGATGAAATCATTGAATA
TCAGCAAATGCTACAGAGTCTGAGAGGGAAACTTAAAAATGCCCAACTTGATGCAGACAAAAGTAACATC
ATGGCTCTGAAACAGGGTATCCAGGAGCGAGACAGTCAAATTAAGATGCTTACTGAGCAAGTAGAACAGT
ATACGAAAGAAATGGAAAAAAATACGTTTATTATTGAAGATTTGAAAAATGAACTCCAAAAAGACAAAGG
TACTTCAAACTTTTATCAGCAGACTCATTATATGAAAATTCACTCAAAAGTACAAATTTTAGAAGAGAAA
ACAAAAGAGGCCGAGAGAATAGCTGAGCTGGCTGAGGCTGATGCCAGGGAGAAGGACAAAGAATTGGTTG
AGGCTCTGAAGAGATTAAAAGATTATGAATCTGGAGTATATGGCTTAGAAGATGCTGTTATCGAAATAAA
GAATTGTAAAGCCCAAATTAAAATAAGAGATGGAGAGATGGAAGTGTTGACCAAGGAGATCAATAAGCTT
GAGATGAAGATCAATGACATCCTTGATGAAAACGAAGCCCTTAGAGAGCGGGCTGGCCTTGAACCCAAGA
CAATGATTGATTTGACTGAATTTAGAAACAGCAAACGGTTAAAGCAGCAACAGTACAGAGCTGAGAACCA
GGTTCTTTTGAAAGAGATCGAAAGTCTAGAGGAGGAGCGTCTTGACTTGAAAAGAAAGATTCGTCAAATG
GCTCAAGAAAGAGGCAAAAGGAACGCAGCCTCAGGATTAACCATTGACGACTTGAACTTATCTGAAACCT
TTTCTCATGAAAATAAAATAGAAGGAAGAAAACTAAATTTTATGAGCCTCAATAATATGAATGAAACACA
ATCAAAGAATGAGTTTCTTTCAAGAGAATTGGCTGAAAAGGAAAAAGATTTAGAAAGAAGTAGGACAGTA
ATTGCTAAATTCCAGAGTAAACTAAAAGAATTAGTCGAAGAAAATAAGCAACTTGAAGAAGGTATGAAAG
AAATTTTGCAAGCTATTAAGGACATGCCGAAGGATTCTGATGTGAAAGGAGGTGAAACATCTTTAATCAT
CCCGAGTCTTGAAAGACTGGTTAACGCTATGGAATCAAAGAATGCAGAAGGAATCTTCGATGCCAGCTTG
CATCTAAAAGCCCAAGTTGACCAACTTACAGGAAGGAATGAAGAATTAAGGCAAGAACTCAGGCAATCTC
GGAAAGAGGCTGTAAATTATTCACAGCAGTTGGTAAAAGCAAACTTAAAGATTGACCACCTTGAGAAGGA
AACTGACCTTCTACGTCAGTCTGCAGGCTCCAATGTAGTATACAAAGGCATAGACTTACCCGATGGGATA
GCACCATCCAGTGCCTATATCATCAATTCTCAGAATGAATACTTAATACATCTTTTGCAGGAACTAGACA
ATAAAGAAAAGAAATTAAAACATTTAGAAGATTCCCTTGAAGATTATAACAGAAAGTTTGCAGTGATTCG
TCATCAACAAAGTTTATTATATAAAGAATACCTAAGTGAAAAGGATATTTGGAAAACAGACTCTGAGATG
ATAAGAGAGGAGAAGAGAAAACTGGAAGATCAAGCTGAGCAGGATGCTGTGAAAGTAAAAGAGTACAACA
ATTTGCTCAGTGCTCTTCAGATGGATTCGAACGAGATGAAGAAAATGCTCTCAGAAAACAGTAGGAAAAT
CACCGTTCTGCAAGTGAACGAGAAGTCCCTCATCCGGCAGTACACCACCTTAGTGGAGATGGAGCGGCAC
CTGAGAAAGGAAAACGGGAAACACAGGAATGACGTCATAGCCATGGAAGCCGAAGTCACTGAAAAACTTG
GAAGTTTGCAAAGATTCAAGGAAATGGCCATCTTCAAGATTGCAGCTCTTCAGAAGGTTATAGATAATAG
TGTTTCTTTGTCTGAATTAGAACTAGCCAATAAACAGTACAATGAGCTGACTACTAAGTACAGGGACATC
TTGCAGAAAGATAATATGCTTGTTCAAAGAACAAGTAACTTAGAACACCTGGAGTGTGAGAATGCATCGC
TAAAAGAACAGATGGAGGCTATCAGTAAAGAGCTGGAGATTACAAAGGAAAAACTCCATACCATTGAACA
GGCCTGGGAACAAGAAACGAAGTTAGGAAATGACTCAAACATGGATAAGGCTAAGAAATCAATGACAAAC
AGTGACATTGTTTCTATTTCAAAGAAAATCACTGTGTTGGAGATGAAGGAATTAAATGAAAGGCAGAGGG
CTGAACACTGTCAGAAAATGTATGAGCACTTAAGGACTTCATTAAAGCAAATGGAAGAACGTAATTTTGA
ATTGGAAACCAAGTTCACTGAGCTTACTAAAATCAACCTGGATGCACAAAAGGTGGAGCAGATGTTGAGA
GACGAATTAGCTGATAGTGTGACCAAGGCAGTAAGTGATGCTGACCGACAGCGGATTCTAGAACTAGAGA
AGAGTGAAGTGGAGCTCAAAGTTGAAGTGTCCAAGCTGAGAGAGATTTCAGATATTGCCAAAAGACAAGT
TGATTTTTTGAATTCGCAACAACAGTCCAGGGAAAAGGAAGTGGAATCCCTCAGAACGCAGCTGCTGGAC
TTCCAGGCACAATCTGACGAAAAGGCTCTAATTGCCAAATTGCACCAACATGTTGTCTCTCTTCAAATTA
GTGAGGCCACTGCCCTCGGTAAGTTAGAGTCAGTTACGTCCAAACTCCAGAAGATGGAAGCCTACAATTT
GCGCTTAGAACAGAAACTGGATGAAAAAGAGCAGGCGCTCTACTATGCTCGTTTGGAAGGTAGAAACAGA
GCAAAACACCTGCGCCAAACCATTCAGTCGCTTCGAAGACAGTTCAGTGGAGCTCTACCCTTAGCACAGC
AGGAAAAGTTCTCCAAAACGATGATTCAGTTGCAAAATGACAAACTTAAGATAATGCAAGAAATGAAGAA
TTCGCAACAGGAACACAGAAATATGGAAAACAAAACACTGGAGTTGGAATTAAAATTAAAAGGCTTAGAA
GAATTGATCAGTACTTTAAAGGATGCCAGGGGAGCCCAGAAGGTAATCAATTGGCATGTGAAAATAGAAG
AACTTCGCCTCCAAGAACTTAAGCTAAATAGAGAACTAGTCAAGGGTAAAGAAGAAATCAAATATTTGAA
TAATATCATCTCTGAATATGAGCATACAATCAACAGTCTAGAGGAAGAAATTGTTCAGCAAAGCAAGTTC
CATGAAGAAAGACAGATGGCTTGGGATCAAAGAGAAGTTGAGCTGGAACGCCAGTTAGACATTTTTGATC
ATCAGCAAAATGAAATACTCAGTGCAGCACAAAAGTTTGAAGACTCTACAGGATCAATGCCAGACCCCAG
CTTGCCTCTTCCAAACCAACTTGAAATTGCTCTAAGAAAAATTAAGGAGAATATTCAAGTAATTCTTAAA
ACACAAGCAACTTGCAAGTCACTAGAAGAGAAACTAAAAGAAAAAGAATCTGCTTTACGGTTGGCAGAGC
AAAATATTCTGTCAAGAGACAAAGTAATCAATGAACTGAGGCTTCGATTGCCTGCCACGGCTGATCGAGA
AAAACTTATAGCTGAGCTAGAAAGAAAAGAGCTGGAGCCGAAATCTCATCACACAATGAAAATTGCCCAC
CAAACTATTGCCAACATGCAGGCAAGGTTAAATCACAAGGAAGAAGTATTGAAGAAATACCAGCACCTTC
TGGAGAAGGCCAGAGAGGAGCAAAGAGAAATTGTTAAGAAGCATGAGGAAGACCTTCATGTTCTTCATCA
CAAATTAGAACAACAGGCCGATAATTCACTCAATAAATTCAGACAGACAGCTCAGGATTTACTTAAGCAG
TCTCCTGCTCCAGTTCCCACCAACAAACATTTCATTCGTCTGGCCGAGATGGAGCAGACAGTAGCAGAAC
AAGATGACTCTCTGTCCTCACTTTTGACCAAACTAAAGAAAGTATCAAAAGATTTGGAAAAACAAAAAGA
AATCACTGAGTTAAAAGTCAGAGAGTTTGAAAATACCAAACTACGGCTCCAAGAAACTCATGCCAGTGAG
GTAAAGAAAGTGAAAGCAGAGGTAGAGGACTTAAGGCATGCTCTAGCCCAAGCACACAAGGACTCCCAGA
GTTTAAAGTCTGAACTCCAGGCTCAGAAAGAAGCAAACTCCAGAGCTCCAACAACCACAATGAGGAATCT
TGTAGACAGGCTAAAGAGCCAACTAGCCTTGAAAGAGAAGCAACAAAAGGCACTTAGTCGAGCCCTGTTG
GAACTTCGGTCGGAAATGACAGCAGCAGCTGAGGAACGTATAATCGCTGTAACTTCTCAAAAAGAGGCAA
ATCTCAATGTTCAACAAGTTGTTGAGCGCCATACTAGAGAGCTAAAGTCACAAATTGAAGATTTAAATGA
AAATCTTTTAAAATTGAAAGAAGCTCTTAAAACAAGTAAGAACAAAGAAAATTCACTAGCTGATGATTTA
AATGAATTAAATAATGAACTGCAAAAAAAGCAAAAAGCTTATAATAAAATCCTTAGAGAGAAAGATGGAA
TTGATCAAGAAAATGATGAACTGAGAAGACAGATTAAAAGACTGTCCAGTGGACTGCAGAGCAAAACTTT
GATAGATAACAAGCAAAGTTTAATCGATGAACTTCAAAAGAAAGTTAAAAAACTTGAAAGCCAACTGGAA
AGAAAGGTGGATGACGTAGACATAAAGCCGGTGAAGGAAAAGAGTAGTAAAGAAGAATTAATTAGGTGGG
AAGAAGGTAAGAAATGGCAAACCAAAGTAGAGGGACTACGAAACAGACTAAAGGAGAAGGAAGGAGAAGC
CCACGGCCTGGCAAAGCAGCTGAATACCTTAAAGGAACTTTTTGCCAAAGCTGATAAAGAGAAACTTACT
TTGCAGAAGAAACTGAAAACAACAGGAATGACTGTTGACCAGGTTTTAGGAGTGCGAGCTTTGGAATCTG
AAAAAGAGTTGGAAGAGCTAAAAAAGAAAAATCTGGACCTAGAAAATGACATATTATACATGAGGACCCA
GCAGGCTCTTCCACGAGATTCTGTTGTGGAAGACTTACATTTACAAAATAAATACCTTCAAGAAAAACTT
CATACTTTAGAAAAAAAACTTTCAAAGGAGAAATATTCTCAGTCTTTGACTTCAGAAATAGAGTCAGATG
ATCACTGTCAAAAAGAACAAGAACTTCAGAAGGAAAATTTGAAGTTGTCATCTGAAAACATCGAGCTGAA
ATTTCAACTTGAACAAGCAAATAAAGATTTGCCAAGACTAAAGAATCAAGTGAAAGATTTGAAGGAAATG
TGTGAATTTCTTAAGAAAGGAAAACTGGAACTTGAGCGGAAGCTTGGTCAGGTCAGAGGGGCTGGTAGAA
GTGGGAAGACAATCCCAGAACTAGAAAAAACCATTGGGTTAATGAAGAAAGTAGTTGAAAAAGTCCAAAG
AGAAAATGAACAATTGAAAAAGGCATCAGGAATACTGACTAGTGAAAAAATGGCTACTATTGAGGAAGAA
AATAGAAACTTAAAGGCTGAACTAGAAAAGCTTAAAGCTCACTTTGGACGTCAGTTGAGTATGCAGTTTG
AATCTAAGAACAAAGGTACTGAGAAAATTGTTGCCGAAAATGAACGGCTTCGGAAAGAACTTAAGAAAGA
AATAGAAGCCTCTGAGAAACTGCGGATAGCTAAGAACAACTTAGAGCTGGTGAACGACAAGATGGCAGCT
CAACTCGAAGAAACTGGGAAGAGACTACAGTTTGCAGAAAGTAGAGCCCCACAGCTGGAAGGTGCTGACA
GCAAGAGCTGGAAGTCAATTGTGGTCTCAAGAGTGTATGAGACCAAGATGAAAGAGCTTGAAAGTGACAT
TGCCAAAAAGAATCAAAGTATCACTGACCTTAAACAGCTTGTAAGAGAAGCAACAGAGAGAGAACAGAAA
GCTAAGAAATACACTGAAGACCTTGAACAACAGATTGAGATCCTCAAAAATGTTCCTGAAGGTGCCGAGA
CAGAGCAAGAGCTTATACGGGAACTCCAGCTTCTTAGATTAGCCAATAATCAGATGGATAAAGAAAGGGC
AGAATTAATCCATCAGATAGAAATTAACAAGGACCAAACCAGAGCTGACAGTAGCATACCTGATTCTGAT
CAACTAAAGGAAAAGATAAATGACCTGGAGACACAACTCAGAAAGTTGGAGCTAGAAAAGCAACATTCGA
AGGAGGAAGTTAAAAAGCTGAAAAAAGAACTGGAAAATTTTGATCCTTCATTTTTTGAAGAAATTGAAGA
CCTGAAGTATAATTATAAGGAAGAAGTGAAAAAGAATATCCTATTAGAAGAGAAGCTAAAAAAACTGTCG
GAACAGTTTGGATTTGAACTGCCTAGTCCTCTTGCTGCTTCTGAACACTCGGAAGATGGAGAAAGTCCTC
ATAGTTTCCCTATTTATTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_146009
Insert Size 7440 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_146009.2, NP_666121.2
RefSeq Size 8006 bp
RefSeq ORF 7440 bp
Locus ID 216274
UniProt ID Q6A078
Gene Summary Involved in early and late steps in cilia formation (PubMed:21565611). Its association with CCP110 is required for inhibition of primary cilia formation by CCP110 (By similarity). May play a role in early ciliogenesis in the disappearance of centriolar satellites and in the transition of primary ciliar vesicles (PCVs) to capped ciliary vesicles (CCVs). Required for the centrosomal recruitment of RAB8A and for the targeting of centriole satellite proteins to centrosomes such as of PCM1 (By similarity). Required for the correct localization of ciliary and phototransduction proteins in retinal photoreceptor cells; may play a role in ciliary transport processes (PubMed:16632484). Required for efficient recruitment of RAB8A to primary cilium (By similarity). In the ciliary transition zone is part of the tectonic-like complex (also named B9 complex) which is required for tissue-specific ciliogenesis and may regulate ciliary membrane composition (PubMed:21725307). Involved in regulation of the BBSome complex integrity, specifically for presence of BBS2, BBS5 and BBS8/TTC8 in the complex, and in ciliary targeting of selected BBSome cargos. May play a role in controlling entry of the BBSome complex to cilia possibly implicating IQCB1/NPHP5 (By similarity). Activates ATF4-mediated transcription (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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