Sptan1 (NM_001177667) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2610027H02Rik; Spna-2; Spna2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225331 representing NM_001177667 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCCAAGTGGGGTCAAAGTGCTGGAAACAGCCGAGGACATCCAGGAGAGACGACAGCAAGTCCTGG ATCGGTACCACCGCTTCAAGGAGCTCTCTACCTTACGGCGTCAGAAGCTGGAGGATTCCTATCGGTTCCA GTTTTTTCAAAGAGATGCTGAGGAGTTGGAGAAGTGGATTCAGGAGAAGCTTCAAGTTGCATCTGATGAG AACTACAAAGACCCAACCAACTTGCAGGGAAAGCTGCAGAAGCACCAAGCCTTTGAAGCTGAAGTACAGG CTAACTCAGGAGCCATTGTGAAGCTGGACGAGACAGGAAACTTGATGATTTCTGAAGGGCACTTTGCATC TGAAACCATCCGGACACGTTTAATGGAGCTGCACCGGCAGTGGGAACTGCTTTTGGAGAAGATGCGGGAG AAAGGAATCAAACTGCTGCAGGCACAGAAGCTGGTGCAGTATTTGCGGGAGTGTGAGGATGTAATGGACT GGATCAATGACAAGGAAGCAATTGTGACTTCTGAGGAGCTGGGCCAGGACCTGGAGCATGTAGAGGTGCT ACAGAAGAAGTTTGAAGAGTTTCAGACTGATCTGGCTGCTCATGAAGAAAGAGTTAATGAAGTGAGCCAG TTTGCTGCCAAACTCATCCAGGAGCAGCACCCGGAAGAGGAGCTGATCAAGACCAAGCAGGATGAGGTGA ATGCAGCATGGCAGCGACTGAAAGGCCTGGCTCTTCAAAGGCAGGGCAAGCTGTTCGGTGCTGCTGAGGT CCAGCGCTTTAACAGGGATGTAGATGAGACCATTGGTTGGATTAAGGAGAAAGAGCAGTTAATGGCCTCT GATGACTTCGGCAGAGACTTAGCAAGTGTTCAAGCTCTGCTTCGAAAGCATGAGGGTCTGGAGAGAGATC TTGCTGCTCTAGAGGACAAGGTGAAAGCCCTGTGTGCTGAGGCTGACCGCCTGCAACAGTCACACCCTCT GAGTGCCAGCCAGATCCAGGTGAAGCGAGAGGAGCTGATTACCAACTGGGAACAGATCCGAACTCTGGCA GCAGAGAGACATGCACGGCTTGATGACTCGTACAGGCTTCAGCGCTTTCTTGCTGACTTCCGTGACCTCA CAAGCTGGGTAACTGAAATGAAAGCCCTCATCAATGCAGATGAACTGGCCAATGATGTGGCTGGTGCTGA AGCCCTGCTGGACAGGCATCAAGAGCACAAGGGTGAGATCGACGCTCATGAAGATAGCTTTAAGTCTGCA GATGAGTCTGGGCAAGCTCTGCTTGCGGCTAGTCACTATGCCTCAGATGAAGTGAGGGAGAAGCTGAGCA TCCTCTCCGAGGAGAGAACTGCCCTGCTGGAGCTGTGGGAGCTTCGGAGGCAGCAGTATGAGCAGTGCAT GGACTTGCAGCTCTTCTACCGTGACACTGAGCAGGTGGACAACTGGATGAGCAAGCAGGAGGCATTCCTG CTAAATGAAGACTTGGGTGACTCCTTAGACAGTGTGGAAGCTCTTTTGAAGAAGCATGAGGACTTTGAAA AATCTCTCAGTGCCCAGGAAGAAAAAATCACAGCCCTTGATGAATTTGCAACCAAACTCATCCAAAATAA CCACTACGCAATGGAAGATGTAGCGACTCGACGAGATGCTCTCCTGAGCCGCCGCAATGCCCTTCATGAG CGAGCCATGCATCGCCGGGCACAGCTGGCTGATTCCTTCCATCTGCAGCAGTTCTTCCGTGATTCTGATG AGCTCAAAAGTTGGGTCAACGAGAAGATGAAAACGGCCACCGATGAAGCTTATAAAGATCCATCCAACCT GCAAGGGAAAGTCCAAAAGCATCAGGCTTTTGAGGCTGAGCTTTCAGCAAACCAGAGCCGGATTGATGCC CTTGAGAAAGCTGGGCAGAAGCTAATAGATGTCAACCACTATGCCAAGGAAGAAGTAGCAGCTCGTATGA ATGAGGTCATCAGTTTGTGGAAGAAACTTCTAGAGGCCACAGAACTGAAAGGAATAAAGCTCCGAGAAGC CAACCAGCAGCAACAATTTAATCGAAATGTTGAAGACATTGAATTGTGGCTGTATGAAGTAGAAGGTCAT TTGGCTTCAGATGATTATGGTAAAGACCTCACTAATGTCCAGAACCTCCAGAAGAAGCATGCTCTGCTAG AGGCGGATGTTGCTGCTCACCAGGATCGAATTGATGGCATCACAATTCAGGCCCGCCAGTTCCAAGATGC TGGCCATTTTGATGCCGAAAATATTAAGAAGAAGCAAGAGGCCCTTGTGGCTCGCTATGAGGCTCTTAAG GAACCCATGGTGGCCCGGAAACAGAAGCTGGCAGATTCTCTTCGTCTGCAGCAGCTCTTCCGGGATGTGG AGGACGAAGAAACCTGGATTCGAGAAAAGGAGCCTATTGCTGCATCCACTAACAGAGGCAAAGATCTTAT TGGAGTCCAGAATCTGCTAAAGAAACACCAAGCTTTGCAAGCAGAGATTGCTGGGCATGAACCTCGAATC AAAGCAGTGACACAAAAGGGAAATGCCATGGTGGAGGAAGGCCATTTTGCTGCAGAGGATGTGAAGGCCA AACTGAGTGAGCTCAACCAGAAGTGGGAGGCTCTGAAAGCCAAAGCCTCCCAGCGGAGGCAGGATCTAGA GGACTCGCTGCAGGCCCAGCAGTACTTTGCCGATGCCAATGAGGCTGAGTCCTGGATGCGGGAGAAGGAG CCCATTGTGGGCAGTACGGACTATGGGAAGGATGAAGACTCTGCTGAGGCTCTACTCAAGAAGCATGAAG CTTTGATGTCCGATCTCAGCGCCTATGGCAGCAGCATCCAGGCTCTGCGAGAACAGGCCCAGTCATGCCG GCAACAAGTGGCCCCCATGGATGATGAGACTGGGAAGGAGCTAGTCTTGGCTCTCTATGACTATCAAGAG AAGAGTCCTCGTGAGGTCACCATGAAGAAAGGGGATATCCTCACCTTGCTCAACAGCACAAACAAGGATT GGTGGAAAGTGGAAGTGAATGACCGGCAGGGTTTCGTGCCAGCTGCATATGTGAAGAAGCTGGACCCCGC CCAGTCAGCCTCGAGGGAGAACCTCCTAGAAGAGCAGGGCAGCATTGCTCTGCGGCAGGGGCAAATTGAC AACCAGACACGCATAACTAAGGAGGCCGGCAGTGTATCTCTGCGTATGAAACAGGTGGAAGAACTATATC AGTCTCTGCTGGAACTGGGTGAGAAGAGAAAAGGCATGTTGGAGAAGAGTTGCAAGAAGTTCATGTTGTT CCGGGAAGCAAACGAGTTACAGCAGTGGATCACTGAGAAGGAGGCTGCTCTAACCAATGAGGAGGTTGGC GCTGACTTGGAGCAGGTTGAGGTGCTGCAGAAGAAGTTTGATGACTTCCAGAAGGATCTGAAAGCCAATG AGTCTCGGCTGAAGGACATTAACAAAGTAGCAGAAGACCTGGAGTCTGAAGGTCTGATGGCAGAGGAAGT GCAAGCTGTGCAGCAGCAGGAGGTGTATGGTGCGATGCCCAGGGATGAAGCAGATTCCAAGACGGCCTCC CCATGGAAGTCTGCTCGACTGATGGTCCATACAGTGGCTACCTTCAACTCCATCAAGGAGCTGAATGAGC GTTGGCGGTCCCTGCAACAGCTGGCTGAGGAACGTAGCCAGCTCTTGGGCAGTGCACACGAAGTACAGAG GTTTCACAGAGATGCTGATGAAACTAAAGAATGGATTGAAGAGAAGAATCAGGCTCTGAACACAGACAAC TATGGCCATGATTTAGCTAGTGTCCAGGCCCTGCAGCGCAAACATGAAGGCTTTGAGAGGGACCTTGCAG CTCTTGGTGACAAGGTGAATTCCCTTGGGGAAACAGCCCAAAGGCTGATCCAGTCCCACCCTGAATCTGC AGAGGACTTAAAGGAAAAGTGCACAGAGTTAAACCAGGCCTGGACCAGCCTAGGGAAGCGTGCAGACCAG CGCAAGGCCAAATTGGGTGACTCCCATGATCTGCAGCGCTTCCTTAGCGATTTCCGGGACCTCATGTCTT GGATCAATGGAATACGAGGGTTGGTATCTTCAGATGAACTAGCCAAGGACGTCACTGGAGCTGAGGCTTT GCTGGAGCGACACCAGGAACACCGGACAGAAATTGATGCCAGGGCTGGCACTTTCCAGGCATTTGAGCAG TTTGGGCAGCAGCTGTTAGCTCACGGGCACTATGCCAGCCCGGAGATCAAAGAGAAACTTGATATTCTTG ACCAGGAGCGCACAGACCTGGAGAAGGCCTGGGTCCAGCGCAGGATGATGCTGGACCACTGCCTGGAGTT GCAGCTGTTCCATCGGGACTGTGAACAAGCAGAAAACTGGATGGCTGCCCGGGAGGCCTTCCTAAACACA GAAGACAAAGGAGACTCGCTGGACAGTGTGGAGGCACTGATCAAAAAACATGAAGACTTTGACAAAGCTA TCAATGTCCAGGAAGAAAAGATAGCTGCCCTGCAGGCCTTTGCCGACCAGCTCATTGCCGTTGACCACTA TGCCAAGGGAGACATTGCAAACCGACGCAATGAGGTCCTTGACCGGTGGCGCCGCCTAAAAGCCCAGATG ATTGAAAAAAGGTCAAAACTTGGAGAATCTCAAACACTTCAGCAGTTCAGCCGGGATGTGGATGAGATTG AAGCCTGGATCAGTGAGAAGTTACAGACAGCCAGTGATGAGTCATACAAGGACCCCACCAACATCCAGCT TTCCAAGCTGTTGAGCAAGCACCAGAAGCACCAAGCCTTTGAGGCAGAACTGCACGCCAATGCTGACCGA ATCCGTGGAGTTATCGACATGGGCAACTCCCTCATTGAGCGTGGGGCCTGTGCTGGCAGTGAGGATGCTG TCAAGGCCCGCCTGGCTGCCCTTGCAGACCAGTGGCAGTTCCTGGTGCAGAAATCAGCTGAGAAGAGCCA GAAGCTAAAAGAGGCAAATAAGCAGCAGAACTTCAACACTGGAATCAAAGACTTTGACTTCTGGCTCTCT GAGGTGGAGGCTCTCCTGGCATCGGAAGATTATGGCAAAGACCTGGCTTCTGTGAACAACTTGCTGAAAA AGCATCAGCTGCTAGAGGCAGACATCTCGGCCCACGAGGATCGTCTGAAGGACCTGAACAGCCAGGCTGA CAGCCTGATGACTAGCAGTGCCTTCGACACCTCCCAAGTGAAAGAGAAGCGGGACACCATCAATGGACGC TTCCAGAAGATCAAGAGCATGGCCACCTCCCGAAGAGCGAAACTGAGCGAGTCCCACCGCCTGCACCAGT TCTTCCGGGACATGGACGACGAGGAGTCCTGGATCAAGGAGAAGAAGTTGTTAGTGAGCTCTGAGGACTA TGGCAGAGACCTCACTGGTGTTCAGAATCTGAGGAAGAAACACAAGCGGCTAGAAGCTGAACTGGCTGCA CATGAACCCGCCATTCAGGGTGTCCTGGACACTGGGAAGAAGCTGTCTGATGACAACACCATCGGGCAGG AGGAGATCCAGCAACGGCTTGCACAGTTTGTGGAGCACTGGAAGGAACTAAAGCAGCTGGCGGCTGCGCG GGGCCAGCGGCTAGAGGAGTCCTTGGAGTATCAGCAGTTTGTAGCCAACGTGGAAGAGGAGGAGGCTTGG ATCAATGAGAAGATGACTCTGGTGGCCAGCGAAGACTATGGAGACACTCTTGCTGCTATCCAGGGCTTAC TGAAGAAACACGAAGCTTTTGAGACAGACTTCACTGTCCACAAGGATCGGGTGAATGATGTCTGTACTAA TGGACAAGACCTCATTAAGAAGAACAATCACCATGAGGAGAACATCTCTTCAAAGATGAAGGGTCTGAAT GGAAAGGTGTCTGATCTGGAGAAAGCAGCAGCTCAGAGGAAAGCAAAGCTGGATGAGAACTCAGCCTTCC TTCAGTTCAACTGGAAGGCTGACGTGGTAGAGTCCTGGATTGGTGAAAAGGAGAACAGCTTGAAAACAGA TGACTATGGCCGAGATCTGTCTTCTGTCCAAACTCTGCTCACAAAACAGGAGACATTTGATGCTGGCCTG CAGGCCTTCCAGCAGGAGGGTATTGCCAATATCACTGCCCTCAAAGACCAGCTGCTAGCTGCCAAGCACA TTCAGTCTAAGGCCATCGAGGCCCGACATGCCTCCCTCATGAAGAGGTGGACCCAGCTGTTGGCCAATTC AGCTACCCGGAAGAAGAAGTTGCTGGAGGCCCAGAGTCATTTCCGTAAGGTAGAAGACCTCTTCCTGACC TTTGCCAAAAAGGCATCGGCTTTCAATAGCTGGTTTGAGAATGCAGAGGAAGACCTCACAGACCCAGTGC GCTGCAACTCTCTGGAAGAAATCAAAGCCCTTCGAGAGGCTCACGATGCCTTCCGCTCATCCCTCAGCTC TGCACAGGCTGACTTCAACCAGCTAGCCGAGCTGGACCGCCAGATCAAGAGTTTCCGGGTGGCCTCCAAC CCCTATACCTGGTTCACGATGGAGGCCCTGGAAGAGACCTGGAGAAACCTCCAGAAGATCATTAAGGAAC GAGAACTGGAGCTGCAGAAGGAACAGCGGCGGCAGGAGGAGAATGACAAGCTGCGCCAAGAATTTGCCCA GCATGCCAACGCCTTCCACCAGTGGATCCAGGAAACAAGAACGTATCTCCTCGACGGGTCCTGCATGGTG GAAGAGTCGGGAACTCTGGAATCTCAGCTTGAAGCTACCAAACGCAAGCACCAGGAGATTCGAGCCATGA GAAGTCAGCTGAAGAAGATCGAGGACCTGGGGGCTGCCATGGAGGAAGCCCTCATCCTGGACAACAAGTA CACGGAGCACAGCACTGTGGGCCTGGCCCAGCAGTGGGACCAGTTAGACCAGCTGGGCATGCGCATGCAG CACAACCTGGAGCAGCAGATCCAGGCCAGGAACACAACAGGAGTGACTGAGGAGGCCCTCAAGGAATTCA GCATGATGTTCAAACACTTCGACAAGGACAAGTCTGGCCGGCTGAACCATCAGGAGTTCAAATCCTGTCT TCGCTCCCTGGGCTATGATCTGCCAATGGTTGAGGAAGGAGAGCCTGATCCTGAGTTTGAGGCAATTCTG GACACTGTTGATCCCAACAGGGATGGCCACGTCTCCCTGCAAGAATACATGGCTTTCATGATCAGCCGCG AAACGGAGAATGTCAAGTCCAGTGAAGAGATCGAGAGTGCTTTCCGGGCCCTCAGCTCTGAGGGCAAGCC TTATGTGACCAAGGAGGAGCTCTACCAGAACCTGACCCGGGAACAAGCTGACTATTGTGTCTCCCACATG AAGCCCTATGTGGATGGCAAGGGCCGAGAACTTCCAACTGCCTTCGACTACGTCGAGTTCACCCGCTCAC TCTTTGTGAATTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001177667
Insert Size	7434 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001177667.1, NP_001171138.1
RefSeq Size	8039 bp
RefSeq ORF	7434 bp
Locus ID	20740

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