Notch2 (NM_010928) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI853703; N2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225328 representing NM_010928 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCGCTCTGCGTCCCGCCGCGCTGCGGGCGCTGCTGTGGCTCTGGCTGTGCGGCGCGGGCCCCGCGC ACGCTTTGCAGTGTCGAGGTGGTCAAGAGCCCTGTGTAAATGAAGGGACCTGTGTTACCTACCACAACGG CACAGGCTTCTGCCGATGTCCAGAGGGCTTCTTGGGAGAATATTGCCAACATCGAGACCCCTGTGAGAAA AACCGCTGTCAGAATGGTGGTACTTGTGTGCCGCAGGGCATGTTGGGGAAAGCTACCTGCCGATGTGCTC CAGGATTCACAGGAGAGGACTGCCAATACTCCACCTCTCACCCCTGCTTTGTGTCCCGCCCTTGTCAGAA TGGGGGTACCTGTCACATGCTCAGCCGGGACACCTACGAGTGCACCTGCCAAGTCGGCTTCACAGGGAAG CAGTGTCAGTGGACAGATGCCTGTCTATCTCATCCCTGCGAAAATGGAAGCACCTGTACATCTGTGGCCA GCCAGTTCTCCTGCAAATGCCCTGCAGGCCTCACAGGGCAGAAGTGTGAAGCTGATATCAATGAGTGTGA CATTCCAGGACGCTGCCAACATGGTGGCACCTGCCTCAACCTTCCTGGTTCCTACAGATGCCAATGTCCT CAGGGCTTCACAGGCCAGCACTGTGACAGCCCTTATGTGCCCTGTGCACCCTCGCCCTGCGTCAACGGAG GCACCTGTCGTCAGACTGGCGACTTCACTTTCGAATGCAACTGCCTGCCAGGTTTTGAAGGGAGCACCTG TGAGCGGAATATCGACGACTGCCCCAACCACAAGTGTCAGAATGGAGGAGTTTGTGTGGATGGTGTCAAT ACCTACAATTGCCGCTGTCCCCCTCAGTGGACTGGGCAGTTCTGCACAGAAGATGTGGACGAGTGTCTGT TGCAGCCCAACGCTTGTCAGAATGGAGGCACCTGCACCAACCGAAACGGAGGCTATGGCTGCGTGTGTGT GAACGGCTGGAGCGGAGATGACTGCAGCGAGAATATCGATGACTGTGCCTATGCTTCCTGCACGCCAGGG TCCACCTGCATTGACCGCGTGGCCTCCTTCTCCTGCCTGTGTCCGGAGGGAAAGGCGGGTCTCCTGTGCC ATCTGGATGATGCCTGTATCAGCAACCCTTGTCACAAGGGGGCGCTGTGTGACACCAACCCCCTGAACGG GCAGTACATTTGCACCTGCCCACAGGGCTACAAGGGCGCTGACTGCACAGAAGACGTGGATGAGTGTGCT ATGGCCAACAGTAACCCTTGTGAGCATGCAGGAAAGTGTGTGAATACAGATGGCGCCTTCCACTGTGAGT GTCTGAAGGGCTACGCAGGGCCTCGCTGTGAGATGGACATCAACGAGTGTCATTCAGACCCCTGCCAGAA CGACGCCACCTGCCTGGATAAGATCGGAGGCTTCACCTGTCTGTGCATGCCAGGTTTCAAAGGTGTGCAT TGTGAGCTGGAGGTAAATGAATGCCAGAGCAACCCGTGTGTGAACAACGGGCAGTGTGTGGACAAAGTCA ACCGCTTCCAGTGTCTGTGTCCCCCTGGCTTCACAGGACCAGTGTGCCAGATCGACATTGATGACTGCTC CAGTACTCCCTGCCTGAATGGGGCCAAGTGCATCGATCACCCGAATGGCTATGAATGCCAGTGTGCCACA GGTTTCACTGGCATATTGTGTGATGAGAACATCGACAACTGTGACCCAGATCCTTGCCACCATGGCCAGT GCCAGGATGGGATCGACTCCTACACCTGCATCTGCAACCCTGGGTACATGGGAGCCATCTGTAGTGACCA GATTGATGAATGCTACAGCAGCCCTTGCTTGAACGATGGGCGCTGCATTGACCTGGTGAATGGTTACCAG TGCAACTGCCAACCAGGCACATCAGGCCTTAATTGTGAAATTAATTTTGATGACTGTGCCAGCAACCCTT GTATGCACGGAGTCTGTGTGGACGGCATCAATCGCTACAGCTGTGTGTGCTCTCCGGGATTCACAGGCCA GAGGTGCAATATTGACATTGATGAGTGTGCCTCCAACCCCTGTCGCAAGGGTGCGACGTGCATCAATGAT GTGAATGGTTTCCGGTGTATATGCCCCGAGGGACCGCATCATCCTAGCTGCTACTCACAGGTGAACGAGT GCCTGAGCAATCCCTGCATCCACGGAAACTGTACTGGAGGTCTCAGTGGCTATAAGTGCCTCTGCGATGC AGGCTGGGTTGGTGTCAACTGTGAGGTGGACAAAAATGAGTGTCTTTCTAACCCATGCCAGAATGGAGGA ACGTGCAATAACCTGGTGAATGGCTACAGGTGTACCTGCAAGAAGGGGTTCAAAGGCTACAACTGCCAGG TGAATATAGATGAGTGTGCCTCCAACCCGTGTCTGAACCAAGGAACCTGCTTTGATGACGTCAGTGGCTA CACTTGCCACTGCATGTTGCCTTACACAGGCAAGAACTGTCAAACAGTGTTGGCTCCCTGTTCCCCAAAC CCGTGTGAGAATGCTGCTGTTTGTAAAGAGGCACCCAATTTTGAGAGCTTCAGCTGCTTGTGTGCTCCTG GCTGGCAAGGTAAACGATGTACAGTTGACGTTGATGAGTGTATCTCCAAGCCGTGTATGAACAATGGTGT CTGCCACAACACTCAGGGCAGCTACGTGTGTGAGTGTCCTCCCGGCTTCAGTGGGATGGACTGTGAAGAG GACATCAATGATTGCCTTGCCAACCCCTGCCAGAACGGAGGCTCCTGTGTGGACCATGTGAATACCTTCT CCTGCCAGTGCCATCCTGGCTTCATAGGGGACAAGTGCCAGACAGACATGAATGAGTGTCTGAGCGAGCC CTGTAAGAATGGGGGGACCTGCTCCGACTATGTCAACAGTTACACCTGCACATGCCCTGCGGGCTTCCAC GGAGTCCACTGTGAAAACAACATTGACGAGTGCACTGAGAGCTCCTGCTTCAATGGCGGCACGTGTGTTG ATGGAATCAACTCTTTCTCTTGCTTGTGCCCCGTGGGTTTCACTGGTCCCTTCTGCCTCCATGATATCAA TGAGTGCAGCTCTAACCCGTGCCTGAATGCTGGAACGTGTGTTGATGGTCTGGGTACCTACCGATGCATC TGTCCCTTGGGCTACACCGGGAAAAACTGTCAGACCCTGGTGAACCTCTGCAGCCGGTCTCCGTGTAAAA ACAAAGGAACTTGTGTTCAGGAAAAGGCAAGGCCCCACTGTCTGTGTCCGCCTGGATGGGATGGTGCATA CTGTGATGTGCTCAACGTGTCCTGTAAGGCGGCAGCCTTGCAGAAAGGAGTACCTGTTGAACACTTGTGC CAGCACTCGGGCATCTGTATCAATGCTGGCAACACCCATCACTGCCAGTGCCCCCTGGGCTACACGGGGA GCTACTGCGAGGAGCAGCTTGATGAGTGTGCATCCAATCCATGCCAGCACGGTGCCACCTGCAATGACTT CATCGGAGGATACAGATGTGAGTGTGTTCCAGGATATCAGGGTGTCAACTGTGAGTATGAAGTGGACGAG TGCCAGAACCAACCCTGCCAGAATGGAGGCACCTGCATCGACCTTGTGAACCATTTCAAGTGTTCGTGTC CCCCAGGCACCCGGGGCCTGCTGTGTGAAGAGAACATCGATGAATGTGCTGGGGGCCCCCACTGCCTTAA CGGTGGCCAGTGCGTGGACCGGATTGGAGGCTACACTTGTCGCTGTTTGCCTGGCTTTGCTGGGGAGCGG TGTGAGGGGGACATCAACGAATGCCTGTCCAACCCTTGCAGCTCAGAGGGCAGCCTGGACTGCGTTCAGC TCAAAAATAACTACAACTGTATCTGCCGCAGCGCCTTCACGGGCCGGCACTGTGAAACCTTCCTAGATGT GTGTCCCCAGAAGCCTTGCCTGAACGGAGGGACTTGTGCCGTGGCTAGCAACATGCCCGATGGCTTCATC TGTCGTTGTCCCCCAGGGTTCTCTGGGGCGAGATGCCAGAGCAGCTGTGGACAAGTGAAGTGCAGGAGAG GGGAGCAGTGTATCCACACTGACTCGGGACCTCGCTGCTTCTGCCTGAACCCCAAGGACTGCGAGTCAGG ATGTGCCAGTAACCCCTGCCAGCATGGGGGCACCTGCTATCCTCAGCGCCAGCCTCCTCACTATTCCTGC CGCTGCCCTCCGTCGTTCGGGGGCAGCCACTGCGAACTCTACACAGCCCCCACCAGCACCCCTCCTGCTA CCTGTCAGAGTCAGTACTGCGCCGACAAGGCTCGGGATGGCATCTGTGACGAGGCCTGCAATAGTCATGC CTGCCAGTGGGATGGAGGTGACTGTTCCCTCACTATGGAGGACCCCTGGGCCAACTGCACCTCCACTCTT CGCTGCTGGGAATACATCAACAACCAGTGTGATGAGCAGTGCAACACCGCAGAGTGCCTGTTTGACAACT TTGAGTGCCAGAGGAATAGCAAGACGTGCAAGTATGACAAATACTGTGCAGACCACTTCAAAGACAACCA CTGTGATCAGGGATGCAACAGCGAGGAGTGTGGCTGGGATGGGCTGGACTGCGCTTCGGACCAGCCTGAG AACCTGGCAGAGGGCACCCTGATCATCGTGGTGCTCCTGCCCCCTGAACAGCTGCTGCAGGATTCGCGAA GCTTCTTGAGGGCCCTGGGCACCCTGCTCCACACCAACTTGCGCATTAAACAAGATTCTCAGGGCGCTCT CATGGTGTACCCCTACTTTGGGGAGAAGTCAGCTGCCATGAAGAAGCAGAAGATGACACGCAGGTCTCTT CCTGAGGAGCAGGAGCAGGAGCAGGAGGTGATAGGCTCTAAGATATTTCTGGAGATCGACAACCGACAGT GTGTTCAAGATTCAGACCAGTGCTTCAAAAACACAGATGCGGCAGCTGCTCTCCTGGCTTCTCATGCCAT CCAAGGGACCCTATCCTACCCTCTAGTGTCTGTTTTCAGTGAACTGGAGAGTCCAAGAAACGCCCAGCTT CTCTATCTGCTCGCGGTCGCTGTTGTCATCATCCTGTTCTTCATCCTGCTGGGGGTCATCATGGCCAAGC GGAAGCGCAAGCATGGCTTCCTCTGGCTGCCGGAAGGGTTCACCCTCCGCCGAGACTCTAGCAATCACAA GCGCCGTGAACCTGTGGGACAGGATGCCGTGGGGCTGAAAAATCTCTCCGTGCAAGTGTCAGAGGCTAAC CTGATTGGTTCTGGGACAAGTGAACATTGGGTTGATGATGAAGGACCCCAGCCAAAGAAAGCCAAGGCTG AGGATGAGGCTTTGCTGTCGGAAGATGACCCCATCGATCGACGGCCCTGGACACAGCAGCACCTTGAAGC TGCAGACATCCGCCACACTCCATCCCTGGCACTCACTCCTCCTCAGGCAGAACAGGAGGTGGACGTGCTG GACGTGAATGTCCGAGGCCCAGATGGGTGTACTCCACTGATGCTGGCTTCTCTCCGAGGAGGCAGCTCAG ACCTGAGTGATGAAGACGAAGATGCTGAGGACTCTTCTGCCAACATCATCACAGACTTGGTCTACCAAGG TGCCAGCCTTCAAGCACAGACAGACCGCACTGGCGAGATGGCCCTGCACCTTGCAGCCCGCTATTCGAGA GCTGATGCTGCCAAACGCCTCCTGGATGCTGGTGCGGATGCAAATGCCCAGGACAACATGGGCCGATGTC CTCTTCACGCTGCGGTGGCAGCAGACGCCCAAGGTGTCTTTCAGATTCTGATCCGCAACCGTGTAACCGA TCTGGATGCCAGAATGAACGATGGTACTACCCCCCTGATCCTGGCTGCCCGCCTGGCTGTGGAAGGAATG GTGGCAGAGTTGATCAATTGCCAAGCAGATGTCAATGCAGTGGATGACCATGGAAAATCTGCCCTCCACT GGGCAGCTGCTGTCAATAATGTGGAGGCGACTCTTCTGCTGTTGAAGAATGGGGCCAACAGAGATATGCA GGACAATAAGGAAGAGACACCTTTGTTTCTTGCTGCCCGAGAGGGAAGTTATGAAGCGGCCAAAATCCTG TTAGACCATTTTGCCAACCGGGACATCACTGACCACATGGACCGCCTTCCCCGGGATGTGGCTCGGGACC GCATGCACCATGACATCGTTCGCCTCCTGGACGAGTATAACGTGACTCCCAGCCCTCCGGGAACGGTCTT GACTTCTGCGCTCTCACCTGTCCTCTGTGGGCCCAACAGGTCTTTCCTCAGTCTGAAGCACACCCCAATG GGTAAGAAGGCTAGGCGGCCCAACACCAAGAGCACCATGCCCACGAGCCTGCCTAACCTTGCCAAGGAGG CCAAGGATGCCAAGGGCAGCAGGAGGAAGAAGTGTCTGAACGAGAAGGTCCAGCTGTCCGAGAGCTCAGT GACTCTATCCCCCGTCGATTCGCTCGAGTCTCCTCACACGTATGTCTCCGATGCCACATCCTCTCCCATG ATCACATCCCCTGGAATCTTACAGGCCTCGCCCACCCCCCTGCTGGCTGCTGCCGCCCCGGCTGCCCCAG TGCACACACAGCATGCGCTGTCTTTCTCTAACCTTCATGACATGCAGCCTTTGGCTCCTGGAGCCAGCAC CGTGCTCCCCTCGGTCAGCCAGCTGCTATCCCACCACCACATCGCGCCCCCAGGTAGTAGCAGTGCAGGA AGCTTGGGCAGGTTACATCCAGTTCCTGTCCCAGCAGACTGGATGAACCGTGTGGAGATGAACGAGACCC AGTACAGTGAAATGTTTGGCATGGTCCTGGCTCCTGCAGAGGGAGCCCACCCTGGCATAGCAGCTCCCCA GAGCAGACCTCCGGAAGGGAAGCACATGTCCACCCAGCGGGAGCCCTTGCCTCCCATCGTGACTTTCCAG CTTATCCCAAAAGGCAGCATTGCCCAGGCAGCCGGAGCTCCCCAGACGCAGTCCAGTTGCCCTCCAGCTG TTGCAGGCCCCTTGCCCTCTATGTACCAGATCCCAGAGATGCCCCGTTTGCCCAGTGTGGCTTTCCCACC TACCATGATGCCCCAGCAGGAGGGGCAGGTAGCTCAGACCATTGTGCCAACCTATCATCCTTTCCCAGCC TCTGTGGGCAAGTACCCCACACCCCCTTCCCAACACAGTTACGCCTCCTCAAATGCTGCTGAGCGAACCC CCAGTCATGGTGGTCACCTCCAGGGCGAGCACCCATACCTGACACCATCCCCAGAGTCTCCTGACCAATG GTCAAGCTCTTCACCACACTCTGCATCTGACTGGTCAGATGTGACCACCAGCCCAACTCCTGGAGGTGGT GGAGGCGGTCAGCGGGGACCCGGAACACACATGTCCGAGCCACCACACAGCAACATGCAGGTGTATGCAT GA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_010928
Insert Size	7422 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_010928.2, NP_035058.2
RefSeq Size	10506 bp
RefSeq ORF	7422 bp
Locus ID	18129
UniProt ID	O35516
Gene Summary	Functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination (PubMed:10393120). Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus (PubMed:10393120, PubMed:18710934). Affects the implementation of differentiation, proliferation and apoptotic programs (PubMed:10393120, PubMed:18710934). May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation (By similarity). In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation (PubMed:18710934). Positively regulates self-renewal of liver cancer cells (By similarity).[UniProtKB/Swiss-Prot Function]

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