Map1b (NM_008634) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	A230055D22; AI843217; LC1; MAP5; Mtap-5; Mtap1b; Mtap5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225326 representing NM_008634 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGACCGTGGTGGTGGAAGCCACCGAACCGGAGCCATCGGGCAGCATCGGCAACCCGGCGGCGTCCA CCTCGCCCAGCCTGTCGCACCGCTTCCTAGACAGCAAGTTCTACTTGCTGGTGGTGGTCGGCGAGACCGT AACCGAAGAGCACCTGAGGCGTGCCATCGGCAACATCGAGCTGGGGATCCGATCGTGGGACACAAACCTG ATTGAGTGCAACTTGGACCAGGAACTCAAACTTTTCGTATCTCGACACTCTGCAAGATTCTCTCCTGAAG TTCCAGGACAAAAGATTCTTCATCACCGAAGTGACGTCTTAGAAACAGTCGTTCTGATCAACCCTTCAGA TGAAGCCGTCAGCACTGAGGTGCGTTTGATGATCACTGACGCTGCCCGCCATAAACTGCTGGTGCTCACC GGACAGTGCTTTGAGAACACTGGCGAGCTCATCCTGCAGTCTGGCTCTTTCTCCTTCCAGAACTTCATAG AGATTTTCACAGATCAAGAGATTGGTGAGCTCCTAAGCACCACCCACCCTGCCAACAAAGCCAGCCTCAC CCTCTTCTGTCCCGAGGAGGGAGACTGGAAGAACTCCAACCTTGACAGACACAATCTCCAAGACTTCATC AACATTAAGCTCAACTCAGCTTCTATCTTGCCAGAAATGGAGGGACTTTCTGAGTTCACCGAGTATCTCT CAGAGTCTGTAGAAGTCCCCTCTCCCTTTGACATCCTGGAACCCCCAACCTCGGGCGGCTTTCTGAAGCT CTCCAAGCCTTGCTGTTACATTTTCCCAGGGGGCCGTGGGGACTCGGCCCTGTTTGCAGTGAACGGATTC AACATGCTCATCAACGGAGGATCAGAGAGAAAGTCTTGCTTCTGGAAGCTCATCCGGCACTTGGACCGTG TGGACTCCATCCTGCTCACCCACATTGGGGATGACAATTTGCCTGGGATCAATAGCATGCTGCAGCGCAA GATTGCAGAGCTGGAAGAGGAGCGGTCCCAGGGGTCCACCAGCAACAGTGACTGGATGAAAAACCTCATC TCCCCTGACCTGGGGGTTGTGTTTCTCAACGTACCTGAAAACCTGAAAGACCCAGAACCCAACATCAAGA TGAAGAGAAGCATAGAAGAAGCATGCTTCACTCTCCAGTACTTAAACAAACTGTCCATGAAACCAGAGCC TCTATTTAGAAGTGTAGGCAATACCATTGAGCCTGTCATCCTGTTCCAGAAGATGGGAGTGGGCAAGCTT GAGATGTACGTGCTTAACCCAGTCAAAAGCAGCAAGGAAATGCAGTATTTTATGCAACAGTGGACTGGCA CCAACAAAGACAAGGCCGAACTAATCCTGCCCAATGGTCAAGAAGTAGACATCCCGATTTCCTACTTAAC CTCAGTCTCATCTTTGATTGTGTGGCATCCGGCCAACCCCGCTGAGAAAATCATTCGGGTTCTGTTTCCC GGAAACAGTACCCAGTACAACATCCTAGAAGGACTGGAGAAACTCAAACATCTAGATTTCCTAAAGCAGC CACTGGCCACCCAAAAAGATCTCACTGGCCAGGTGCCCACCCCCCCAGTGAAACAGGTCAAACTGAAACA GCGGGCTGACAGCCGAGAGAGTCTGAAGCCAGCCACGAAACCAGTTGCCAGTAAATCTGTGAGGAAGGAG TCCAAGGAGGAAACCCCTGAAGTCACAAAGACTAGTCAAGTGGAGAAAACACCCAAAGTTGAAAGCAAAG AGAAAGTGTTAGTGAAAAAAGACAAGCCTGTAAAGACAGAAAGTAAACCTTCAGTGACAGAAAAGGAGGT GTCCAGCAAGGAAGAGCAGTCCCCAGTGAAAGCCGAGGTCGCTGAGAAGCAAGCCACTGAGAGCAAACCC AAAGTCACCAAGGACAAAGTGGTAAAAAAGGAAATAAAGACAAAACTCGAGGAAAAGAAAGAGGAGAAGC CCAAGAAAGAAGTGGTTAAGAAGGAAGACAAAACACCCCTCAAGAAAGACGAGAAGCCAAGAAAGGAAGA GGTGAAGAAGGAGATCAAGAAAGAAATCAAAAAAGAAGAGAGAAAGGAGCTGAAGAAAGAGGTGAAGAAG GAAACACCCCTGAAGGATGCCAAGAAGGAGGTGAAGAAAGAGGAGAAGAAAGAAGTTAAAAAGGAAGAGA AGGAACCCAAAAAGGAAATCAAGAAGATCTCCAAGGACATAAAGAAATCAACTCCTCTGTCAGACACAAA GAAGCCATCTGCGTTAAAACCAAAAGTAGCGAAGAAAGAAGAGTCCACTAAGAAGGAGCCTCTTGCTGCT GGGAAACTCAAGGACAAGGGGAAAGTCAAAGTCATTAAAAAGGAAGGCAAGACCACAGAAGCAGCTGCCA CAGCTGTTGGCACTGCTGCCACCACAGCTGCCGTGGTGGCTGCAGCTGGAATAGCGGCCAGTGGTCCTGT CAAAGAACTGGAAGCTGAGAGGTCCCTTATGTCCTCCCCTGAGGATCTAACCAAGGACTTTGAGGAGCTA AAGGCTGAGGAGATTGATGTGGCCAAGGACATCAAGCCTCAGCTGGAGCTCATTGAAGATGAAGAGAAAC TGAAGGAAACCCAGCCGGGAGAAGCCTACGTCATCCAGAAAGAGACGGAAGTCAGCAAAGGTTCCGCTGA GTCACCCGATGAGGGAATCACCACCACCGAGGGGGAGGGCGAGTGTGAGCAAACCCCTGAGGAGCTGGAG CCAGTTGAGAAGCAGGGCGTGGATGACATTGAGAAGTTTGAAGATGAAGGAGCTGGTTTTGAAGAATCCT CAGAGACCGGAGACTACGAAGAGAAGGCGGAAACTGAGGAGGCTGAGGAACCGGAAGAGGACGGGGAAGA CAATGCGAGCGGGAGTGCCTCTAAGCACAGCCCCACAGAAGATGACGAAAGCGCAAAGGCTGAGGCGGAC GTGCACCTCAAGGAGAAGAGGGAGTCTGTGGTCAGTGGTGACGACCGGGCGGAAGAAGACATGGATGATG TGCTTGAGAAAGGAGAAGCTGAACAGTCTGAGGAAGAGGGCGAAGAGGAGGACAAAGCAGAGGATGCCAG AGAGGAAGGCTATGAGCCCGACAAAACTGAGGCTGAGGATTATGTGATGGCCGTGGCTGACAAGGCGGCT GAGGCTGGAGTCACAGAGGAGCAGTACGGATACCTGGGCACGTCGGCCAAGCAACCTGGAATCCAGTCTC CTAGCCGAGAACCTGCATCTTCAATTCATGATGAGACCCTACCTGGAGGCTCTGAGAGCGAGGCCACTGC TTCGGATGAGGAGAATCGGGAAGACCAGCCTGAGGAATTCACTGCTACCTCTGGATATACTCAGTCTACC ATTGAGATATCTAGTGAGCCTACGCCAATGGATGAGATGTCTACTCCTCGTGATGTGATGAGCGACGAGA CCAACAACGAGGAGACAGAGTCCCCATCTCAGGAGTTCGTAAACATTACCAAATATGAGTCCTCGCTGTA TTCTCAGGAGTACTCCAAACCTGCTGTTGCATCATTCAATGGATTGTCAGAAGGGTCAAAAACAGACGCC ACTGATGGTAAGGATTACAACGCTTCCGCCTCCACCATATCACCACCTTCGTCCATGGAAGAAGACAAAT TCAGCAAGTCTGCTCTTCGTGATGCTTACTGCTCAGAAGAGAAAGAGTTGAAAGCCAGTGCCGAGTTAGA CATCAAAGATGTTTCAGATGAGAGACTTAGCCCAGCCAAGAGTCCATCCCTGAGTCCTTCTCCGCCATCC CCCATAGAGAAGACTCCCCTGGGTGAACGTAGCGTGAATTTCTCTCTGACACCCAATGAGATTAAAGTGT CAGCAGAGGGAGAAGCAAGGTCAGTGTCCCCTGGAGTGACCCAGGCAGTAGTTGAAGAACATTGTGCCAG TCCTGAGGAGAAGACCCTGGAAGTAGTGTCGCCATCCCAGTCTGTGACAGGCAGTGCTGGCCACACACCT TACTACCAATCTCCCACCGATGAGAAGTCCAGTCACCTACCCACAGAAGTCACTGAAAAGCCACAGGCAG TTCCAGTGAGCTTTGAATTCAGTGAGGCCAAAGATGAGAACGAGAGAGCGTCCCTAAGCCCCATGGATGA GCCTGTGCCTGACTCAGAGTCTCCTGTGGAGAAGGTTCTGTCTCCTTTACGCAGTCCTCCCCTTCTTGGA TCTGAGTCCCCATATGAAGACTTCCTGAGTGCTGATAGCAAGGTTCTTGGCAGACGTTCCGAAAGCCCCT TTGAAGGGAAGAATGGAAAGCAGGGCTTCCCAGACAGAGAAAGCCCAGTTTCTGACCTGACTTCCACTGG TCTTTACCAAGACAAACAGGAAGAGAAAAGCACGGGCTTCATACCCATAAAGGAAGACTTTGGTCCAGAA AAGAAAACCAGTGATGTTGAAACCATGAGTTCTCAATCAGCTCTAGCTTTGGATGAAAGGAAATTGGGAG GAGATGTATCTCCAACACAAATAGACGTCAGTCAGTTTGGCTCTTTCAAAGAAGACACCAAGATGTCCAT TTCAGAAGGCACTGTTTCAGACAAGTCAGCCACACCTGTGGATGAGGGTGTGGCAGAAGACACCTACTCC CACATGGAGGGTGTGGCCTCCGTCTCTACTGCCTCTGTGGCTACAAGTTCGTTTCCAGAGCCAACCACAG ATGACGTGTCTCCTTCTCTGCATGCCGAGGTGGGTTCTCCACATTCCACAGAAGTTGATGACTCCCTGTC CGTTTCTGTGGTGCAAACACCTACCACGTTCCAGGAAACAGAAATGTCTCCATCTAAAGAAGAGTGCCCA AGACCGATGTCAATTTCTCCTCCAGACTTCTCCCCCAAGACAGCCAAATCCAGGACACCAGTTCAAGATC ACCGATCTGAACAGTCTTCTATGTCTATTGAGTTCGGCCAGGAATCCCCTGAGCATTCTCTTGCTATGGA CTTTAGTCGGCAGTCTCCAGATCACCCTACTCTGGGTGCCAGTGTGCTTCATATCACTGAAAACGGGCCA ACTGAAGTGGACTATAGTCCCTCTGATATCCAGGACTCTAGTTTGTCGCATAAGATCCCGCCAACGGAAG AGCCATCCTACACCCAGGATAATGATCTGTCTGAGCTCATCTCTGTGTCTCAGGTAGAGGCTTCCCCGTC CACCTCTTCAGCTCACACCCCTTCTCAGATAGCCTCTCCTCTTCAGGAAGACACTCTCTCTGATGTTGTT CCTCCCAGAGAGATGTCCTTATATGCCTCACTTGCGTCTGAAAAAGTGCAGAGCCTGGAAGGAGAGAAGC TCTCTCCGAAATCTGATATTTCTCCACTCACCCCACGAGAGTCCTCACCTTTATATTCACCTGGCTTTTC AGATTCTACCTCTGCAGCTAAAGAGACTGCAGCAGCTCACCAAGCCTCCTCTTCCCCACCAATAGATGCA GCAACAGCAGAGCCCTATGGCTTCCGCTCCTCAATGCTATTTGATACAATGCAACATCATCTGGCCTTGA ATAGAGATTTGACTACATCTAGTGTGGAGAAAGACAGCGGAGGGAAGACACCCGGAGACTTTAACTATGC CTATCAAAAGCCTGAGAATGCCGCCGGATCTCCAGATGAAGAAGATTATGACTATGAATCTCAAGAGAAA ACCATCCGGACCCACGACGTGGGTGGTTACTACTATGAGAAGACAGAGAGAACCATAAAATCCCCATGTG ACAGTGGGTACTCCTATGAGACCATTGAGAAGACCATCAAGACCCCAGAAGATGGTGGCTACACCTGTGA AATTACCGAGAAAACCACTCGGACCCCAGAAGAGGGTGGCTATTCATATGAGATCAGCGAGAAGACGACA CGGACCCCTGAAGTAAGCGGCTACACCTATGAAAAGACTGAGAGGTCCAGAAGGCTCCTGGATGACATTA GCAATGGCTATGATGACACTGAGGATGGCGGCCACACACTTGGCGACTGTAGCTACTCCTATGAAACCAC TGAGAAAATTACCAGCTTTCCTGAGTCTGAAAGCTATTCCTACGAGACAAGTACAAAAACAACACGGAGT CCAGACACCTCTGCATACTGTTACGAGACCATGGAGAAGATCACCAAGACCCCACAGGCATCCACATACT CCTATGAGACCTCAGACCGATGCTACACTACGGAAAAGAAGTCCCCCTCAGAGGCACGCCAGGATGTTGA TTTATGTCTGGTGTCCTCCTGTGAATTCAAGCATCCCAAGACGGAGCTCTCGCCTTCCTTCATTAATCCA AATCCTCTTGAGTGGTTTGCTGGTGAAGAGCCCACTGAAGAATCAGAGAAGCCCCTCACTCAGTCTGGAG GAGCCCCCCCACCTTCAGGAGGAAAACAACAGGGCAGGCAATGCGATGAAACGCCACCCACCTCAGTCAG TGAGTCAGCCCCATCCCAGACAGACTCTGATGTTCCCCCAGAGACGGAAGAGTGCCCCTCCATCACAGCC GACGCCAATATTGACTCTGAAGATGAGTCAGAAACTATCCCCACAGACAAAACCGTCACATACAAACACA TGGACCCACCTCCAGCCCCCATGCAAGACCGAAGCCCTTCTCCTCGCCACCCCGACGTGTCCATGGTGGA CCCAGACGCCTTGGCCGTTGATCAGAACCTAGGCAAGGCTCTGAAAAAAGACCTGAAGGAGAAGACCAAG ACCAAGAAGCCAGGCACAAAGACCAAGTCCTCTTCACCTGTCAAAAAGGGTGATGGGAAGTCCAAGCCTT TAGCAGCTTCCCCCAAACCAGGAGCTTTGAAGGAATCCTCCGACAAGGTGTCCAGAGTGGCTTCTCCCAA GAAGAAAGAATCCGTGGAAAAAGCTACAAAGACCACCACCACTCCTGAGGTCAAAGCCACACGCGGAGAA GAGAAGGACAAGGAGACCAAGAACGCAGCTAACGCCTCTGCATCCAAATCAGCGAAGACCGCAACGGCAG GACCAGGAACCACTAAGACAGCCAAGTCGTCCACCGTGCCTCCAGGCCTCCCTGTGTATTTGGACCTGTG CTATATTCCCAACCACAGCAACAGTAAGAATGTCGATGTTGAGTTCTTCAAGAGAGTGAGGTCATCTTAC TACGTGGTGAGTGGGAACGACCCTGCCGCGGAGGAGCCCAGCCGGGCTGTCCTGGATGCCTTGTTGGAAG GGAAGGCTCAGTGGGGAAGCAACATGCAGGTGACTCTGATTCCGACACATGACTCCGAGGTGATGAGGGA GTGGTACCAGGAGACCCACGAGAAGCAGCAGGACCTCAACATCATGGTCTTAGCAAGCAGCAGTACAGTG GTCATGCAAGACGAGTCCTTCCCCGCATGCAAGATAGAACTGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI
ACCN	NM_008634
Insert Size	7395 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_008634.2, NP_032660.2
RefSeq Size	11852 bp
RefSeq ORF	7395 bp
Locus ID	17755
UniProt ID	P14873
Gene Summary	Phosphorylated MAP1B may play a role in the cytoskeletal changes that accompany neurite extension. Possibly MAP1B binds to at least two tubulin subunits in the polymer, and this bridging of subunits might be involved in nucleating microtubule polymerization and in stabilizing microtubules. Acts as a positive cofactor in DAPK1-mediated autophagic vesicle formation and membrane blebbing (By similarity). Facilitates tyrosination of alpha-tubulin in neuronal microtubules. Required for synaptic maturation.[UniProtKB/Swiss-Prot Function]

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