Map1b (NM_008634) Mouse Untagged Clone

CAT#: MC225326

Map1b (untagged) - Mouse microtubule-associated protein 1B (Mtap1b), (10ug)



  "NM_008634" in other vectors (2)

CNY 24,408.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms A230055D22; AI843217; LC1; MAP5; Mtap-5; Mtap1b; Mtap5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC225326 representing NM_008634
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGACCGTGGTGGTGGAAGCCACCGAACCGGAGCCATCGGGCAGCATCGGCAACCCGGCGGCGTCCA
CCTCGCCCAGCCTGTCGCACCGCTTCCTAGACAGCAAGTTCTACTTGCTGGTGGTGGTCGGCGAGACCGT
AACCGAAGAGCACCTGAGGCGTGCCATCGGCAACATCGAGCTGGGGATCCGATCGTGGGACACAAACCTG
ATTGAGTGCAACTTGGACCAGGAACTCAAACTTTTCGTATCTCGACACTCTGCAAGATTCTCTCCTGAAG
TTCCAGGACAAAAGATTCTTCATCACCGAAGTGACGTCTTAGAAACAGTCGTTCTGATCAACCCTTCAGA
TGAAGCCGTCAGCACTGAGGTGCGTTTGATGATCACTGACGCTGCCCGCCATAAACTGCTGGTGCTCACC
GGACAGTGCTTTGAGAACACTGGCGAGCTCATCCTGCAGTCTGGCTCTTTCTCCTTCCAGAACTTCATAG
AGATTTTCACAGATCAAGAGATTGGTGAGCTCCTAAGCACCACCCACCCTGCCAACAAAGCCAGCCTCAC
CCTCTTCTGTCCCGAGGAGGGAGACTGGAAGAACTCCAACCTTGACAGACACAATCTCCAAGACTTCATC
AACATTAAGCTCAACTCAGCTTCTATCTTGCCAGAAATGGAGGGACTTTCTGAGTTCACCGAGTATCTCT
CAGAGTCTGTAGAAGTCCCCTCTCCCTTTGACATCCTGGAACCCCCAACCTCGGGCGGCTTTCTGAAGCT
CTCCAAGCCTTGCTGTTACATTTTCCCAGGGGGCCGTGGGGACTCGGCCCTGTTTGCAGTGAACGGATTC
AACATGCTCATCAACGGAGGATCAGAGAGAAAGTCTTGCTTCTGGAAGCTCATCCGGCACTTGGACCGTG
TGGACTCCATCCTGCTCACCCACATTGGGGATGACAATTTGCCTGGGATCAATAGCATGCTGCAGCGCAA
GATTGCAGAGCTGGAAGAGGAGCGGTCCCAGGGGTCCACCAGCAACAGTGACTGGATGAAAAACCTCATC
TCCCCTGACCTGGGGGTTGTGTTTCTCAACGTACCTGAAAACCTGAAAGACCCAGAACCCAACATCAAGA
TGAAGAGAAGCATAGAAGAAGCATGCTTCACTCTCCAGTACTTAAACAAACTGTCCATGAAACCAGAGCC
TCTATTTAGAAGTGTAGGCAATACCATTGAGCCTGTCATCCTGTTCCAGAAGATGGGAGTGGGCAAGCTT
GAGATGTACGTGCTTAACCCAGTCAAAAGCAGCAAGGAAATGCAGTATTTTATGCAACAGTGGACTGGCA
CCAACAAAGACAAGGCCGAACTAATCCTGCCCAATGGTCAAGAAGTAGACATCCCGATTTCCTACTTAAC
CTCAGTCTCATCTTTGATTGTGTGGCATCCGGCCAACCCCGCTGAGAAAATCATTCGGGTTCTGTTTCCC
GGAAACAGTACCCAGTACAACATCCTAGAAGGACTGGAGAAACTCAAACATCTAGATTTCCTAAAGCAGC
CACTGGCCACCCAAAAAGATCTCACTGGCCAGGTGCCCACCCCCCCAGTGAAACAGGTCAAACTGAAACA
GCGGGCTGACAGCCGAGAGAGTCTGAAGCCAGCCACGAAACCAGTTGCCAGTAAATCTGTGAGGAAGGAG
TCCAAGGAGGAAACCCCTGAAGTCACAAAGACTAGTCAAGTGGAGAAAACACCCAAAGTTGAAAGCAAAG
AGAAAGTGTTAGTGAAAAAAGACAAGCCTGTAAAGACAGAAAGTAAACCTTCAGTGACAGAAAAGGAGGT
GTCCAGCAAGGAAGAGCAGTCCCCAGTGAAAGCCGAGGTCGCTGAGAAGCAAGCCACTGAGAGCAAACCC
AAAGTCACCAAGGACAAAGTGGTAAAAAAGGAAATAAAGACAAAACTCGAGGAAAAGAAAGAGGAGAAGC
CCAAGAAAGAAGTGGTTAAGAAGGAAGACAAAACACCCCTCAAGAAAGACGAGAAGCCAAGAAAGGAAGA
GGTGAAGAAGGAGATCAAGAAAGAAATCAAAAAAGAAGAGAGAAAGGAGCTGAAGAAAGAGGTGAAGAAG
GAAACACCCCTGAAGGATGCCAAGAAGGAGGTGAAGAAAGAGGAGAAGAAAGAAGTTAAAAAGGAAGAGA
AGGAACCCAAAAAGGAAATCAAGAAGATCTCCAAGGACATAAAGAAATCAACTCCTCTGTCAGACACAAA
GAAGCCATCTGCGTTAAAACCAAAAGTAGCGAAGAAAGAAGAGTCCACTAAGAAGGAGCCTCTTGCTGCT
GGGAAACTCAAGGACAAGGGGAAAGTCAAAGTCATTAAAAAGGAAGGCAAGACCACAGAAGCAGCTGCCA
CAGCTGTTGGCACTGCTGCCACCACAGCTGCCGTGGTGGCTGCAGCTGGAATAGCGGCCAGTGGTCCTGT
CAAAGAACTGGAAGCTGAGAGGTCCCTTATGTCCTCCCCTGAGGATCTAACCAAGGACTTTGAGGAGCTA
AAGGCTGAGGAGATTGATGTGGCCAAGGACATCAAGCCTCAGCTGGAGCTCATTGAAGATGAAGAGAAAC
TGAAGGAAACCCAGCCGGGAGAAGCCTACGTCATCCAGAAAGAGACGGAAGTCAGCAAAGGTTCCGCTGA
GTCACCCGATGAGGGAATCACCACCACCGAGGGGGAGGGCGAGTGTGAGCAAACCCCTGAGGAGCTGGAG
CCAGTTGAGAAGCAGGGCGTGGATGACATTGAGAAGTTTGAAGATGAAGGAGCTGGTTTTGAAGAATCCT
CAGAGACCGGAGACTACGAAGAGAAGGCGGAAACTGAGGAGGCTGAGGAACCGGAAGAGGACGGGGAAGA
CAATGCGAGCGGGAGTGCCTCTAAGCACAGCCCCACAGAAGATGACGAAAGCGCAAAGGCTGAGGCGGAC
GTGCACCTCAAGGAGAAGAGGGAGTCTGTGGTCAGTGGTGACGACCGGGCGGAAGAAGACATGGATGATG
TGCTTGAGAAAGGAGAAGCTGAACAGTCTGAGGAAGAGGGCGAAGAGGAGGACAAAGCAGAGGATGCCAG
AGAGGAAGGCTATGAGCCCGACAAAACTGAGGCTGAGGATTATGTGATGGCCGTGGCTGACAAGGCGGCT
GAGGCTGGAGTCACAGAGGAGCAGTACGGATACCTGGGCACGTCGGCCAAGCAACCTGGAATCCAGTCTC
CTAGCCGAGAACCTGCATCTTCAATTCATGATGAGACCCTACCTGGAGGCTCTGAGAGCGAGGCCACTGC
TTCGGATGAGGAGAATCGGGAAGACCAGCCTGAGGAATTCACTGCTACCTCTGGATATACTCAGTCTACC
ATTGAGATATCTAGTGAGCCTACGCCAATGGATGAGATGTCTACTCCTCGTGATGTGATGAGCGACGAGA
CCAACAACGAGGAGACAGAGTCCCCATCTCAGGAGTTCGTAAACATTACCAAATATGAGTCCTCGCTGTA
TTCTCAGGAGTACTCCAAACCTGCTGTTGCATCATTCAATGGATTGTCAGAAGGGTCAAAAACAGACGCC
ACTGATGGTAAGGATTACAACGCTTCCGCCTCCACCATATCACCACCTTCGTCCATGGAAGAAGACAAAT
TCAGCAAGTCTGCTCTTCGTGATGCTTACTGCTCAGAAGAGAAAGAGTTGAAAGCCAGTGCCGAGTTAGA
CATCAAAGATGTTTCAGATGAGAGACTTAGCCCAGCCAAGAGTCCATCCCTGAGTCCTTCTCCGCCATCC
CCCATAGAGAAGACTCCCCTGGGTGAACGTAGCGTGAATTTCTCTCTGACACCCAATGAGATTAAAGTGT
CAGCAGAGGGAGAAGCAAGGTCAGTGTCCCCTGGAGTGACCCAGGCAGTAGTTGAAGAACATTGTGCCAG
TCCTGAGGAGAAGACCCTGGAAGTAGTGTCGCCATCCCAGTCTGTGACAGGCAGTGCTGGCCACACACCT
TACTACCAATCTCCCACCGATGAGAAGTCCAGTCACCTACCCACAGAAGTCACTGAAAAGCCACAGGCAG
TTCCAGTGAGCTTTGAATTCAGTGAGGCCAAAGATGAGAACGAGAGAGCGTCCCTAAGCCCCATGGATGA
GCCTGTGCCTGACTCAGAGTCTCCTGTGGAGAAGGTTCTGTCTCCTTTACGCAGTCCTCCCCTTCTTGGA
TCTGAGTCCCCATATGAAGACTTCCTGAGTGCTGATAGCAAGGTTCTTGGCAGACGTTCCGAAAGCCCCT
TTGAAGGGAAGAATGGAAAGCAGGGCTTCCCAGACAGAGAAAGCCCAGTTTCTGACCTGACTTCCACTGG
TCTTTACCAAGACAAACAGGAAGAGAAAAGCACGGGCTTCATACCCATAAAGGAAGACTTTGGTCCAGAA
AAGAAAACCAGTGATGTTGAAACCATGAGTTCTCAATCAGCTCTAGCTTTGGATGAAAGGAAATTGGGAG
GAGATGTATCTCCAACACAAATAGACGTCAGTCAGTTTGGCTCTTTCAAAGAAGACACCAAGATGTCCAT
TTCAGAAGGCACTGTTTCAGACAAGTCAGCCACACCTGTGGATGAGGGTGTGGCAGAAGACACCTACTCC
CACATGGAGGGTGTGGCCTCCGTCTCTACTGCCTCTGTGGCTACAAGTTCGTTTCCAGAGCCAACCACAG
ATGACGTGTCTCCTTCTCTGCATGCCGAGGTGGGTTCTCCACATTCCACAGAAGTTGATGACTCCCTGTC
CGTTTCTGTGGTGCAAACACCTACCACGTTCCAGGAAACAGAAATGTCTCCATCTAAAGAAGAGTGCCCA
AGACCGATGTCAATTTCTCCTCCAGACTTCTCCCCCAAGACAGCCAAATCCAGGACACCAGTTCAAGATC
ACCGATCTGAACAGTCTTCTATGTCTATTGAGTTCGGCCAGGAATCCCCTGAGCATTCTCTTGCTATGGA
CTTTAGTCGGCAGTCTCCAGATCACCCTACTCTGGGTGCCAGTGTGCTTCATATCACTGAAAACGGGCCA
ACTGAAGTGGACTATAGTCCCTCTGATATCCAGGACTCTAGTTTGTCGCATAAGATCCCGCCAACGGAAG
AGCCATCCTACACCCAGGATAATGATCTGTCTGAGCTCATCTCTGTGTCTCAGGTAGAGGCTTCCCCGTC
CACCTCTTCAGCTCACACCCCTTCTCAGATAGCCTCTCCTCTTCAGGAAGACACTCTCTCTGATGTTGTT
CCTCCCAGAGAGATGTCCTTATATGCCTCACTTGCGTCTGAAAAAGTGCAGAGCCTGGAAGGAGAGAAGC
TCTCTCCGAAATCTGATATTTCTCCACTCACCCCACGAGAGTCCTCACCTTTATATTCACCTGGCTTTTC
AGATTCTACCTCTGCAGCTAAAGAGACTGCAGCAGCTCACCAAGCCTCCTCTTCCCCACCAATAGATGCA
GCAACAGCAGAGCCCTATGGCTTCCGCTCCTCAATGCTATTTGATACAATGCAACATCATCTGGCCTTGA
ATAGAGATTTGACTACATCTAGTGTGGAGAAAGACAGCGGAGGGAAGACACCCGGAGACTTTAACTATGC
CTATCAAAAGCCTGAGAATGCCGCCGGATCTCCAGATGAAGAAGATTATGACTATGAATCTCAAGAGAAA
ACCATCCGGACCCACGACGTGGGTGGTTACTACTATGAGAAGACAGAGAGAACCATAAAATCCCCATGTG
ACAGTGGGTACTCCTATGAGACCATTGAGAAGACCATCAAGACCCCAGAAGATGGTGGCTACACCTGTGA
AATTACCGAGAAAACCACTCGGACCCCAGAAGAGGGTGGCTATTCATATGAGATCAGCGAGAAGACGACA
CGGACCCCTGAAGTAAGCGGCTACACCTATGAAAAGACTGAGAGGTCCAGAAGGCTCCTGGATGACATTA
GCAATGGCTATGATGACACTGAGGATGGCGGCCACACACTTGGCGACTGTAGCTACTCCTATGAAACCAC
TGAGAAAATTACCAGCTTTCCTGAGTCTGAAAGCTATTCCTACGAGACAAGTACAAAAACAACACGGAGT
CCAGACACCTCTGCATACTGTTACGAGACCATGGAGAAGATCACCAAGACCCCACAGGCATCCACATACT
CCTATGAGACCTCAGACCGATGCTACACTACGGAAAAGAAGTCCCCCTCAGAGGCACGCCAGGATGTTGA
TTTATGTCTGGTGTCCTCCTGTGAATTCAAGCATCCCAAGACGGAGCTCTCGCCTTCCTTCATTAATCCA
AATCCTCTTGAGTGGTTTGCTGGTGAAGAGCCCACTGAAGAATCAGAGAAGCCCCTCACTCAGTCTGGAG
GAGCCCCCCCACCTTCAGGAGGAAAACAACAGGGCAGGCAATGCGATGAAACGCCACCCACCTCAGTCAG
TGAGTCAGCCCCATCCCAGACAGACTCTGATGTTCCCCCAGAGACGGAAGAGTGCCCCTCCATCACAGCC
GACGCCAATATTGACTCTGAAGATGAGTCAGAAACTATCCCCACAGACAAAACCGTCACATACAAACACA
TGGACCCACCTCCAGCCCCCATGCAAGACCGAAGCCCTTCTCCTCGCCACCCCGACGTGTCCATGGTGGA
CCCAGACGCCTTGGCCGTTGATCAGAACCTAGGCAAGGCTCTGAAAAAAGACCTGAAGGAGAAGACCAAG
ACCAAGAAGCCAGGCACAAAGACCAAGTCCTCTTCACCTGTCAAAAAGGGTGATGGGAAGTCCAAGCCTT
TAGCAGCTTCCCCCAAACCAGGAGCTTTGAAGGAATCCTCCGACAAGGTGTCCAGAGTGGCTTCTCCCAA
GAAGAAAGAATCCGTGGAAAAAGCTACAAAGACCACCACCACTCCTGAGGTCAAAGCCACACGCGGAGAA
GAGAAGGACAAGGAGACCAAGAACGCAGCTAACGCCTCTGCATCCAAATCAGCGAAGACCGCAACGGCAG
GACCAGGAACCACTAAGACAGCCAAGTCGTCCACCGTGCCTCCAGGCCTCCCTGTGTATTTGGACCTGTG
CTATATTCCCAACCACAGCAACAGTAAGAATGTCGATGTTGAGTTCTTCAAGAGAGTGAGGTCATCTTAC
TACGTGGTGAGTGGGAACGACCCTGCCGCGGAGGAGCCCAGCCGGGCTGTCCTGGATGCCTTGTTGGAAG
GGAAGGCTCAGTGGGGAAGCAACATGCAGGTGACTCTGATTCCGACACATGACTCCGAGGTGATGAGGGA
GTGGTACCAGGAGACCCACGAGAAGCAGCAGGACCTCAACATCATGGTCTTAGCAAGCAGCAGTACAGTG
GTCATGCAAGACGAGTCCTTCCCCGCATGCAAGATAGAACTGTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_008634
Insert Size 7395 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_008634.2, NP_032660.2
RefSeq Size 11852 bp
RefSeq ORF 7395 bp
Locus ID 17755
UniProt ID P14873
Gene Summary Phosphorylated MAP1B may play a role in the cytoskeletal changes that accompany neurite extension. Possibly MAP1B binds to at least two tubulin subunits in the polymer, and this bridging of subunits might be involved in nucleating microtubule polymerization and in stabilizing microtubules. Acts as a positive cofactor in DAPK1-mediated autophagic vesicle formation and membrane blebbing (By similarity). Facilitates tyrosination of alpha-tubulin in neuronal microtubules. Required for synaptic maturation.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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