Rif1 (NM_175238) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	5730435J01Rik; 6530403D07Rik; AU016181; AW549474; D2Ertd145e
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225307 representing NM_175238 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGCCCCAGGTCGCAGCCCCCTGGAGCCGCTTCTGGAGACTTGGGAAGACCCCTCGGTGCCTCCGG GAGAGCAGACGGACGCCTACCTAACTCTGACCAGTCGTATGACTGGAGAAGAAGGAAAAGAAGTCATTGC AGAAATCGAGAAAAACCTTTCTCGGCTGTACACGGTTTTAAAGGCTCACATTTCCAGTCAGAACTCAGAA CTGAGCAGTGCTGCTTTGCAAGCCTTGGGATTTTGTTTATATAATCCCAGAATTACCTCAGGATTGTCAG AGGCAAATATTCAAGAACTGCTTTTAACCTTGAATGGTATCATTAAGAGTTCAGACAAAAATGTGTGTAC CAGGGCACTGTGGGTAATATCCAAACAGACGTTTCCTGCTGAACTTGTTAGCAAAATGGTATCCAGTATT ATTGATTCATTGGAAGTGATACTAAGTAAAGGAGAGATACATTCTGCTGTTGTTGATTTTGAAGCATTAA ATGTTATTATAAGTCATTGGTTTCTTCATTGTAGGCTAATTGAACAAGCCCCAGTTCAAATGGGAGAAGA GTCTGTGAGGTGGGCCAAATTGGTCATACCATTAGTTGTTCACTCAGCACAAAAAGTACATTTGCGGGGA GCCACTGCTCTAGAGATGGGGATGCCATTGCTGCTTCAGAAACAACAAGAAATAGCCTTGATTACAGAGC ACCTTATGACTACTAAATTAATCTCAGAACTCCAAAAACTATTTAAGAATAAAAATGAGACTTATGTGTT AAAACTATGGCCTTTGTTTGTCAAACTTCTTGGAAAGACCTTGCACCGAAGTGGGAGTTTCATCAACTCT TTGTTACAGCTGGAAGAACTGGGCTTCCGTAGTGGAACACCCATGATTAAAAAAATCGCTTTCATTGCTT GGAAAAGCTTAATAGATAATTTTGCTTTAAACCCAGATATACTTTGTAGTGCAAAAAGACTGAAGTTGTT AATGCAACCCTTGAGTTCCATCCATGTGAGAACAGAAACTTTAGCATTAACAAAACTAGAAGTCTGGTGG TATTTACTGATGAGACTTGGACCTCAGCTCCCTGCCAATTTTGAACAGGTGTGCGTGCCACTGATTCAGA GTACAATAAGTGTTGATAGTATTCCTTCACCTCAAGGCAATTCATCTCGAGGGTCTGCTTCTCCAGGCTT AAGTCCACTGACTCCGGGACATAAAGGTGCTTCTCCTTATGGAAGTCCCCGTGGGAACCTGAGTTCAAAT ACAGGTGGAATGGCTGCAATCCCTTCTATTCAGCTTTTGGGACTGGAAATGATGCTTCATTTTTTGTTGG GCCCAGAAGTTTTGAGTTTTGCCAAGCAACACAAGATTGTATTGAGTTTAGAGCCACTGGAACACCCGCT AATCAGCAGCCCTTCTTTCTTCTCCAAGTATGCGCATACACTTATCACTGCTGTTCATGATAGCTTTGTC TCAGTTGGAAAGGATGCCTCAGATGCAGTAGTCAGTGCTATCTGGAAGGAGCTAATTAGCTTGGTGAAGT CAGTTACTGAAGCAGGAAACAGAAAAGAAAAATCAGGTTCTGAAGTTTTGACCCTTCTACTAAAATCTTT GGAAAACATAGTGAAGTCAGAAGTATTTCCTGTATCAAAAACACTGGTTCTCATGGAAATTACAGTTAAA GGACTTCCTCCGAAAGTACTAGGTTCACCAGCATATCAGGTTGCTAATATGGATATTCTTAATGGAACTC CAGCATTGTTTTTAATCCAGTTAATTTTCAACAACAATCTTTTAGAATGTGGTGTAGAAGATGAGAAGTT TTTTCTCAATCTAGAAACACTTGTAGGCTGTGTACTTTCTGGTCCAACATCACCACTGGCTTTTAGTGAT TCTGTTCTAACTGTGATTAATCAGAATGCAAAGCAGTTGGTAAACAAGGAGCATCTCTGGAGAATGTGGA GTATGATAGTCAGTCCACTGACTGATGTGATTCACCAGACCAATGAAGTGAATCAAGGCGATGCCTTAGA ACATAATTTTAGTGCCATCTATGGTGCATTGACTTTACCAATAAACCATATTTTTTCAGCTCAGACATTT CCCACGGGTACCATGAAAGCTTTACTTAAAACTTGGTCAGAATTGTATAGAGCATTTACTCGCTGTGCTT CTTTGGTGGCAACAGCAGAAGAGAATCTGTGCTGTGAAGAACTTTCTTCTAAAATAATGTGCAGTTTGGA AGATGAAGTCCTTTCAGATTTGCTATTCTTGGACAGGATCTCTCATATTATTATTGTAATGGTTGATTGT ATCGACTTTTCACCATATAATAAGAAATACCAGCCAAAAATCAAATCACCGCAGAGATCTTCAGATTGGT CCAGAAAGAAGAAGGAGCCTCTAGGGAAATTGGCTTCTTTATTTAAGCTAATTGTGAAAGTCATCGATAC TTTCCACACTCTAAGCCTCAAGGAAACATTCTCTGATACACTGCTTGCTATTGGTAACTCTATCATTAGC ATGCTTTCCAATGTATTTGGACATATTTCCTTGCCCTCTATGATCCGAGAAATATTTGCAACTTTTACAA GACCTCTGGCTTTACTGTATGAAAACTCAAAGCTGGATGAGGCTCCTAAAGTGTATACTAGTCTGAACAA CAAGTTAGAAAAGCTATTGGGAGAAATTGTTGCATGCCTACAGTTCAGCTATCTTGGAGCCTATGACAGT GAACTTCTTGAACATCTTTCTCCACTCCTGTGCGTAATATTTCTTCACAAGAATAAACAGATTCGGAAAC AGAGTGCTCTGTTGTGGAATGCCACATTTGCTAAAGCGACAGCTCTGGTTTATCCTGAGGAATTAAAACC AATATTAAGACAAGCCAAACAGAAAATTTTGCTTCTGCTGCCTGGTTTGGAAAATGTTGAAATGATGGAT GAGTCCAGTGAACCGTATTCAGAATCAACAGAGAATTCACAATTAAATGTCAAGATAAGTGGCATGGAAA GAAAATCAAGTGGAAAAAGAGATTCCATTTTGGCACATACAAAGGATAAAAAAAAAAAAGTGAAACTGTC GGCCAAACTGAAACTAGAATCTTCATCTCCAAAAATAAAGAGTGGTAAGCTTTTGGAAGAGGAAAAGTCT ACCGACTTTGTCTTCATTCCTCCAGAAGGAAAAGAGACAAAGGCAAGGGTTCTAACTGAGCATCAGAAAG AAGTACTCAAAACAAAACGGTGTGATATTCCTGCCTTGTATAATAATCTGGATGCTTCACAGGACACTTT ATTTTCTGCTCAGTTTAGTCAAGAAGAATCAATGGAAAGCCTCACTTTAACTGAAAAACCAAAAGAAGAT GCCAAGATAATTAAGGAAGAGCAAATGGAAAGTACTATTTTCATCCATCAAGATGCCCCGGAGAACTGTG GAATAGATGAACATTCTGAGAATGCTTCTTTACCAAATTGTGGTGGCTCTGTTGCTGAAACCAATCCAGA AACATTGATCACTGGTTTTGATGCTAGAAAAGAAGTATTAATTTCATCAAAGATATTGTCTGCTGAAAGT TCATCTAGTACAGAAACTTCGGTGGTCAGCAGTAGTTCAGTTTCTAATGCCACTTTTTCTGGAACTCCTC CACAGCCTACAAGTCGGAGACAAACCTTTATTACTTTGGAGAAATTTGATGGCTCAGAAACTAGACCTTT TAGTCCATCCCCCTTGAATAACATATCTTCCACTGTTACAGTGAGAAATAACCAGGATAACACAACTAAC ACTGACATGCCACCAAAAGCAAGGAAAAGAGAAGTGACGAACTCAAAATCTGATTCAGAAAATTTAGCGA ATGCAGGTAAGAAATCAAGTCGGAGATGGAGTAAAGCTGAGCAGTCAGTTACTAAAAAGTCTAAGCCATC ACTGACATCTGAACAGGAAGAGCACTCATCCGAAAATAACTCTCCTGATCTGCTCAGCCCAACAGAACAT GTGTCAGAAAATGATGATCATCCTTCTGAAGCTACCCTAGAGCATAAAGATGGAGATCCTAAACCAGCAG TAGAAAATGCTTCATTGGAAGACTTAACAACAGAAGAGAAAAATGTAGGCATTAATATGGAATCTAAAGA AAGTACAGCCTCAGTTGTAGCACGAACAGAACAAATAGTAAATGAAGATAGTCAGGCTGCTGCACTAGCC CCAAATCCAAAAACACTCCGACGGTCTTCGAGGCGGCGTTCAGAAGCTGTAGATTCTTGCAGTGACAGCC AAGAGAGAGAGAGTGGTCAGCAAAAAAAGGAAAGACGAAAGGAAGAAGAAAAAATAATCTCCAAGAGTCC GTTGCGTATCAAAGATGATAAGTTGCCCACGCAAAAACTAACTGATGAGTCACCTATACAGGAAAATTTA ACTGAAAAGGGAAATACTTTACCTGAGAGAACTTCAGGGGAACCCAGTGTTAATGCTGAAATTGACCAAA ATAGAAGAAAACCAGACCTTGAGAATGTTAGTTCTGAAGGAGGTGGTGGTACCCTGGACAATCTAGACAA GTCGTCTGAGAAACCTTTAAGAGGACGGACACGTTATCAAACAAGAAGAGCTTCGCAGGGTTTGATTTCT GCTGTTGAAAACTCAGAATCTGACAGTTCTGAGGCAAAGGAAGAAGTTTCTAGAAAGAAACGATCAGGGA AATGGAAAAATAGAAGCAGTGACAGTGTTGACATTGAAGAACAAGAAGAAAAAAAGGCTGAAGAGGAAGT TATGAAAACTGCAAATCAGACACTCGATGGCCAGGCAGTTCCTGATGTTGATGTAAATGCAGCGGCTCAG GTTTGTGAAAAAAGTACAAATAACAACAGGGTCATCCTCCAGGATTCTGCTGGGCCTGCAGATTCACTGC AAGCTCCACCCAAAGGCGAGGAGAAAAGTAAGATTAACAAATGTGTAGACAGTTCATTTGTAAGTCTACC TGTGCCAGAGTCAAACCTCAGGACTAGGAATGCCAGTAAGAGATTATTATATAAACAAGATAATGATAGT AATGTGAGGGTATCAGACAGCTCTCTGTCTCCCGAAAAATTCACCCAAGTTGAATGCCAACACAAGAGAA GTAGGAGAGTCAGGAGATCTAAAAGTTGTGACTGCTGTGGCGAAAAATCACAGTCCCAGGAAAAGTCATT TATTGGGTTAAAGAACACAGAAAGTTATGCTATAAAGAGTGTGGAGAAAAAAAAGACAGATCTACAAGTA CCTGAGACTGCCCCTGAAACTCGTGAAGCTCGTGACCATGCTGAAACAAAGTTGGCAGGCGAAGAGCCTC TTGTGAATTTTCATGTGGGTCTTAAAGAAGAGAATTGTACTACTGGTGATTCAGTTAAGTCTGAGGCTGA GTTGCAAGAAGCTTCCCTTCCACCTGAAATAGTAACTGTGAAAGAGAAGACTTACGATACAGACGCTAGT GAAGCAGTGTCTGAAATCCAAGGGCCATGTAGTGAGAACCACAGCCCTGCTGAGGACCCAGGCTTAAGTG AGTGCAAAGACATTTCACAGAAGCAGCTTTCAGAGAACGGAGAGCTTGACATCAGCGATGTAGGGAAGGC ATGCAAAGTCATAGCTGGGTCCAGTCCAGAGGGAGTTGAAACTATGGAATTAAATGTAAGAAATGATGCA TTTGTAGCTGCTGACTCTGAAAAGAGTACCCAAATGGATGTTTCTGTAGATGTGGCAACAGAGGAAGATA ATAAAAAAGATGAATGTGAAGCAGTCACAACTGAAGTGAATGTTGAAGGAGTAGCCACTGAGGACTTTAA TTCAGGTATGGATCTTTCTGATACTCCCATACCTGTAAGCAAGGATGTTGAGACTGAGCACGCAGCTAGT GGGGAAATAGAGGGGGAGAGCAATGAATCTGATTCAGGCTCATGTGAGGAAATGAACAAAGAAATGGGAA GTCACAAAGCCCAGATGAGCACTGAAATTGACAGTGCCAGGGTAAAGGAGACGGACATACTTGCCAGTGC CTCTAAATCTGAAGAAGCGCTGATTGGAAGACTAGACGTCAACACCCAGAGCTTTGTTAGTGACATTGAA ATGAGTTCTGGAGAAAGAACTGTTAATTGCAAAACTGAGACATCCATTGAACTTAATAAATTAGATGAAG CAAAATTAAGTGGCAATGAAGCTACAGTGGGAAATGATACTCTGCAGGAAGTTTGCTTTACTTCAGAGAA AGTGGAGAAATTACCACAGTGTCTGCTAGTTCAAGTGGCTTCTGAACTTGGAGCAGAAAGCAATACTACG TCTCCCGAAAAGTTAGAGCTGGATTCTTTTGGATCAGTAAATGAAAGTCCTAGTGGTATGCAGCAGGCAC GTTGTGTCTGGTCACCTTTGGCTTCTCCATCCACAAGCATTTTAAAGAGAGGACTAAAAAGATCCCAAGA AGATGAGATCTCACCAGTTAACAAGATTAGACGTGTCTCGTTTGCAGATCCAATATACCAAGCAGGATTG GCAGATGACATTGATAGGCGTTGCTCTGTTGTTAGATCTCATTCTTCAAATAGCTCTCCCATAATAAAAA GTGTTAAAACTTCACCTACTTCACACTCTAAGCATAATACCACTTCAGCCAAAGGATTTCTGTCCCCAGG ATCACAGAGCTCTAAATTTAAGAGCCCAAAGAAGTGTTTAATTACAGAAATGGCCCAAGAATCCATGCTG TCCCCAACAGAAAGTGTTTACCCAGCTTTGGTGAACTGTGCAGCATCAGTTGACATCATTTTACCTCAGA TTACATCAAATATGTGGGCAAGAGGTCTTGGGCAACTCATCAGAGCCAAGAATATAAAAACCATTGGGGA TTTGAGTACGCTTACGGCATCTGAAATAAAAACTCTGCCTATCCGTTCTCCAAAGGTGTTTAATGTAAAA AAGGCCCTCAGAGTATACCATGAGCAACAGATGAAATCTCGTGGACTTGAAGAAATTCCAATTTTTGATA TTTCTGAGAAGGCAGTAAATGGAGTGGAGAGCAGGACTGTCTCCACGGATGAAGAAAGGTTCGCCTCAGA TTTGATTGAACCTGTGACCCTAGATACCCCATTGTCTAAAAATCTTGTGGCACAAATTAGTGCTCTTGCT CTTCAACTGGACTCAGAAGATCTCTACAGTTACACTGGAAGCCAGCTATTTGAAATGCATGAAAAACTTG GTACCATGGCAAACTCTATAATAAGAAATCTACAGTCACGTTGGAGATCACCAGCCCATGAAAATTCTTA G ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_175238
Insert Size	7281 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_175238.5, NP_780447.4
RefSeq Size	8502 bp
RefSeq ORF	7281 bp
Locus ID	51869
UniProt ID	Q6PR54
Gene Summary	Key regulator of TP53BP1 that plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage: acts by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs (PubMed:23333305, PubMed:23306437, PubMed:23306439). In response to DNA damage, interacts with ATM-phosphorylated TP53BP1 (PubMed:23333305, PubMed:23306437, PubMed:23306439). Interaction with TP53BP1 leads to dissociate the interaction between NUDT16L1/TIRR and TP53BP1, thereby unmasking the tandem Tudor-like domain of TP53BP1 and allowing recruitment to DNA DSBs (By similarity). Once recruited to DSBs, RIF1 and TP53BP1 act by promoting NHEJ-mediated repair of DSBs (PubMed:23333305, PubMed:23306437). In the same time, RIF1 and TP53BP1 specifically counteract the function of BRCA1 by blocking DSBs resection via homologous recombination (HR) during G1 phase (PubMed:23333305, PubMed:23306437). Also required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs (PubMed:23333305, PubMed:23333306, PubMed:23306439). Promotes NHEJ of dysfunctional telomeres (PubMed:23333305).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1 and 2 both encode the same isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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