Cacna1b (NM_001042528) Mouse Untagged Clone

CAT#: MC225275

Cacna1b (untagged) - Mouse calcium channel, voltage-dependent, N type, alpha 1B subunit (Cacna1b), transcript variant 1, (10ug)



  "NM_001042528" in other vectors (2)

CNY 37,240.00


货期*
13周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms alpha(1B); AW050276; AW060892; AW822256; BIII; Cav2.2; Cchn1a
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC225275 representing NM_001042528
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTCCGCTTCGGGGACGAGCTAGGCGGCCGCTATGGGGGCACCGGCGGCGGGGAGCGGGCTCGGGGTG
GCGGGGCCGGCGGGGCGGGTGGCCCGGGCCAGGGGGGTCTGCCGCCGGGCCAGCGGGTCCTGTACAAGCA
GTCCATTGCGCAGCGCGCACGGACTATGGCCCTGTACAACCCCATCCCAGTCAAGCAGAACTGCTTCACC
GTCAACCGCTCGCTCTTCGTCTTCAGCGAGGACAACGTCGTCCGCAAATACGCTAAGCGCATCACCGAAT
GGCCGCCCTTCGAATACATGATCCTGGCCACCATCATCGCCAACTGCATTGTTCTGGCCCTGGAGCAGCA
CCTCCCTGATGGGGACAAGACTCCCATGTCTGAGCGACTAGATGACACGGAGCCTTACTTCATCGGGATC
TTTTGCTTCGAGGCGGGCATCAAGATCATAGCCCTGGGCTTTGTTTTCCACAAGGGCTCCTACCTTCGGA
ACGGCTGGAATGTCATGGACTTCGTGGTGGTCCTCACGGGGATTCTCGCCACAGCTGGAACTGACTTTGA
CCTGCGCACACTGAGGGCTGTGCGTGTGCTTAGGCCCCTGAAGCTGGTGTCTGGAATTCCAAGCTTGCAG
GTGGTGCTTAAGTCCATCATGAAGGCCATGGTCCCGCTGCTGCAGATTGGGCTGCTGCTCTTCTTTGCCA
TCCTCATGTTTGCCATCATCGGCCTCGAATTCTATATGGGCAAATTCCATAAGGCCTGTTTCCCCAACAG
CACAGATACAGAGCCTGTGGGTGACTTTCCCTGTGGCAAAGATCCCCCTGCTCGTCAGTGTGATGGTGAC
ACCGAATGCCGGGAGTACTGGCCAGGACCCAACTTTGGCATCACCAATTTTGACAACATCCTGTTTGCCA
TCTTGACAGTGTTCCAGTGTATCACCATGGAGGGCTGGACTGACATCCTCTACAATACAAATGATGCGGC
TGGCAACACATGGAACTGGTTGTACTTCATCCCCCTCATCATCATTGGCTCCTTCTTCATGCTCAACCTG
GTGCTGGGTGTGCTTTCCGGAGAGTTTGCCAAGGAGCGGGAGCGAGTCGAGAACCGCCGCGCCTTCCTGA
AGCTCCGCAGGCAGCAGCAGATTGAGCGAGAGCTGAATGGGTACTTGGAGTGGATCTTCAAGGCAGAGGA
AGTCATGTTGGCAGAGGAGGACAAGAATGCAGAAGAGAAATCCCCTTTGGATGTGTTGAAGAGAGCTGCC
ACCAAGAAGAGCCGAAATGACCTCATCCATGCAGAAGAGGGGGAGGACCGGTTTGTAGACCTCTGTGCAG
TTGGGTCTCCATTTGCTCGTGCCAGCCTCAAGAGTGGGAAGACGGAGAGCTCATCGTACTTCCGGAGAAA
GGAGAAGATGTTCCGGTTCTTTATCCGGCGTATGGTGAAAGCACAGAGCTTCTACTGGGTGGTACTGTGT
GTGGTGGCCCTGAACACACTGTGTGTGGCCATGGTGCACTATAATCAGCCTCAGCGGCTTACCACTGCAC
TGTACTTTGCAGAGTTTGTTTTCCTGGGTCTCTTCCTCACAGAGATGTCCCTGAAGATGTATGGCCTAGG
GCCCAGAAGTTACTTCAGGTCTTCCTTCAACTGCTTTGACTTTGGGGTGATTGTGGGGAGTATCTTTGAA
GTAGTCTGGGCTGCCATCAAGCCAGGAACCTCCTTTGGAATCAGTGTGCTGCGGGCTCTGCGACTGCTGA
GGATATTCAAAGTTACCAAGTATTGGAACTCTCTGAGGAACCTGGTGGTTTCCCTCCTCAATTCCATGAA
GTCCATCATCAGCCTTCTCTTCCTGCTTTTCCTCTTCATCGTGGTCTTCGCTCTGTTGGGGATGCAGCTG
TTTGGGGGACAGTTCAACTTTCAAGATGAGACTCCAACCACCAATTTTGATACCTTCCCAGCTGCCATCC
TCACTGTCTTTCAGATCCTGACAGGAGAGGATTGGAATGCCGTAATGTATCATGGGATTGAGTCGCAAGG
TGGAGTCAGCAAAGGCATGTTTTCTTCCTTTTACTTCATCGTCCTGACACTGTTTGGAAACTACACCCTG
CTGAATGTTTTTCTGGCCATTGCTGTGGACAACCTTGCCAATGCCCAGGAGTTGACCAAGGATGAAGAGG
AGATGGAAGAAGCAGCCAATCAGAAACTTGCTCTTCAGAAGGCCAAAGAAGTAGCTGAAGTCAGCCCCAT
GTCTGCTGCCAATATCTCCATCGCTGCCAGGCAGCAGAACTCGGCCAAGGCGCGCTCAGTATGGGAGCAG
CGGGCCAGTCAGCTAAGGCTCCAGAATCTGCGTGCCAGCTGTGAGGCATTGTACAGTGAGATGGACCCTG
AGGAGCGCCTGCGTTATGCCAGCACGCGCCATGTGAGGCCAGACATGAAGACACACATGGACCGACCCCT
AGTGGTGGAGCCTGGTCGAGATGGCTTGCGGGGACCCGTTGGGAGCAAGTCAAAGCCTGAAGGCACGGAG
GCCACAGAAAGCGCGGACCTACCTCGCAGGCACCACCGGCACCGTGATAGGGACAAGACCTCAGCCACAG
CACCTGCTGGAGGCGAACAGGACAGGACAGAAAGCACCGAGACCGGGGCCCGGGAGGAACGTGCGCGCCC
TCGTCGAAGTCACAGCAAGGAGACTCCAGGGGCTGACACGCAAGTGCGCTGTGAGCGCAGTAGACGTCAC
CACCGGCGCGGCTCCCCGGAGGAGGCCACTGAACGGGAGCCTCGGCGCCACCGTGCCCACCGGCATGCAC
AGGACTCAAGCAAGGAGGGCACGGCGCCGGTGCTTGTACCCAAGGGTGAGCGACGAGCAAGACACCGAGG
CCCACGCACGGGTCCACGTGAGGCAGAGAACAACGAGGAGCCCACACGCAGGCACCGTGCAAGGCATAAG
GTGCCACCCACACTGCAGCCCCCAGAGAGGGAGGCTGCAGAGAAGGAGAGCAACGCGGTGGAAGGGGATA
AGGAAACCCGAAATCACCAGCCCAAGGAACCTCACTGTGACCTGGAGGCCATTGCAGTTACAGGTGTGGG
CCCTCTGCACATGCTGCCCAGCACCTGTCTCCAGAAAGTGGACGAGCAACCAGAGGATGCAGACAACCAG
CGTAATGTCACCCGGATGGGCAGTCAGCCCTCAGATCCCAGCACCACTGTGCATGTCCCAGTGACACTGA
CAGGCCCTCCTGGGGAGACCCCTGTAGTTCCCAGTGGTAACATGAACCTGGAAGGCCAAGCAGAGGGCAA
GAAGGAGGCAGAGGCGGATGATGTGCTGAGAAGAGGCCCCAGGCCCATCGTTCCCTACAGCTCCATGTTT
TGTCTCAGCCCCACCAACCTGCTTCGTCGCTTCTGCCATTACATTGTGACCATGCGGTACTTTGAGATGG
TAATTCTTGTGGTCATTGCCTTGAGCAGCATTGCCCTGGCTGCAGAGGATCCTGTGCGGACAGATTCATT
CAGGAACAACGCTTTAAAGTACATGGATTACATCTTTACAGGAGTCTTCACCTTTGAAATGGTCATAAAG
ATGATAGACTTGGGCTTGCTGCTGCACCCTGGTGCCTACTTCCGGGACCTGTGGAACATTCTGGACTTCA
TTGTTGTCAGTGGAGCCCTGGTGGCATTTGCGTTCTCGAGCTTCATGGGAGGATCCAAAGGGAAAGACAT
CAATACCATCAAGTCTCTGAGAGTCCTGCGTGTCCTGAGGCCCCTCAAGACCATCAAGCGGCTGCCTAAG
CTCAAGGCTGTCTTTGACTGTGTGGTGAACTCCCTGAAGAACGTCTTGAACATCCTGATTGTCTACATGC
TCTTCATGTTCATATTTGCCGTCATTGCCGTCCAGCTCTTCAAAGGGAAGTTCTTTTACTGTACTGATGA
ATCCAAGGAGCTGGAGAGGGACTGCCGGGGTCAGTATTTGGATTATGAGAAGGAAGAAGTAGAAGCCCAG
CCAAGGCAGTGGAAGAAATATGACTTCCACTATGACAATGTTCTCTGGGCCTTGTTGACGCTGTTCACAG
TGTCCACGGGAGAGGGGTGGCCCATGGTGCTGAAACACTCTGTGGATGCCACCTATGAGGAACAGGGGCC
CAGTCCCGGGTTCCGGATGGAGCTCTCCATCTTCTACGTGGTCTACTTTGTGGTCTTCCCTTTTTTCTTT
GTCAACATCTTTGTGGCCTTGATCATTATCACCTTCCAGGAACAGGGAGATAAGGTGATGTCTGAATGCA
GCTTAGAAAAGAATGAGAGGGCTTGCATTGATTTTGCCATCAGTGCCAAGCCCCTGACACGGTACATGCC
TCAAAACAAACAGTCGTTCCAGTATAAGACATGGACATTCGTGGTCTCTCCACCCTTTGAGTACTTCATC
ATGGCTATGATAGCCCTCAACACAGTGGTGCTGATGATGAAGTTCTATGATGCACCTTATGAGTACGAGC
TGATGCTGAAATGCCTGAACATTGTCTTCACATCCATGTTCTCGATGGAGTGCATACTGAAGATCATCGC
CTTTGGGGTATTGAACTACTTCAGAGATGCCTGGAACGTCTTTGACTTTGTCACGGTTTTGGGAAGTATT
ACTGATATTTTAGTAACAGAGATAGCGAACAACTTCATCAACCTAAGCTTCCTTCGCCTCTTCCGGGCGG
CACGGCTGATCAAGCTGCTTCGCCAGGGCTACACCATCCGCATCCTATTGTGGACCTTCGTCCAGTCCTT
TAAGGCGCTGCCCTACGTGTGCCTCCTCATTGCCATGCTGTTCTTCATCTACGCCATCATCGGCATGCAG
GTTTTTGGAAACATTGCCCTTGATGATGACACCAGTATCAACCGACACAACAACTTCCGGACATTTCTGC
AAGCCTTAATGCTATTGTTCAGGAGTGCCACTGGGGAGGCCTGGCATGAGATCATGCTGTCTTGTCTGGG
CAACCGGGCCTGTGACCCACATGCCAACGCCAGTGAGTGCGGGAGCGACTTTGCCTATTTTTATTTTGTC
TCCTTCATCTTCCTCTGTTCCTTTCTGATGTTGAACCTCTTTGTTGCTGTAATCATGGACAATTTTGAGT
ACCTCACTCGGGACTCTTCCATCCTAGGGCCTCACCACTTAGACGAATTCATTCGAGTCTGGGCTGAATA
CGACCCAGCTGCGTGTGGGCGCATCAGTTACAATGACATGTTTGAGATGCTGAAACACATGTCCCCACCT
CTGGGGTTGGGGAAGAAATGCCCGGCTCGAGTTGCATACAAGCGCCTGGTTCGCATGAACATGCCCATAT
CCAATGAGGACATGACGGTGCACTTTACGTCCACACTGATGGCCCTCATCCGGACAGCACTGGAGATCAA
GCTTGCCCCAGCGGGGACGAAGCAGCACCAGTGTGATGCTGAGCTGAGAAAAGAGATCTCTTCTGTGTGG
GCTAATCTGCCCCAGAAGACTCTGGACTTACTGGTACCACCCCACAAACCTGACGAGATGACAGTGGGGA
AGGTCTATGCTGCTCTCATGATATTTGACTTCTACAAACAGAACAAAACCACCAGAGATCAGACTCACCA
AGCTCCCGGAGGCCTGTCCCAGATGGGTCCCGTTTCCCTGTTCCACCCTCTGAAGGCCACCCTGGAACAG
ACACAGCCCGCTGTGCTTCGAGGAGCTCGGGTTTTCCTTCGGCAAAAGAGTGCAACTTCCCTCAGCAATG
GGGGTGCCATACAAACCCAGGAAAGTGGCATCAAGGAGTCGCTGTCCTGGGGCACGCAGAGGACCCAAGA
TGCACTTTATGAGGCCAGAGCACCTCTAGAACGTGGCCATTCTGCAGAGATCCCTGTGGGGCAGTCAGGA
ACACTGGCTGTGGATGTCCAGATGCAGAACATGACACTGAGAGGACCAGATGGGGAGCCCCAGCCTGGGC
TGGAAAGCCAAGGCAGAGCTGCCTCTATGCCACGCCTAGCGGCAGAAACACAGCCGGCCCCTAATGCCAG
CCCCATGAAGCGCTCCATCTCCACACTGGCTCCACGCCCACATGGGACTCAGCTTTGCAGCACAGTTCTG
GACCGGCCTCCTCCTAGCCAGGCATCACATCACCACCACCACCGCTGCCACCGGCGCAGAGACAAGAAGC
AAAGGTCCCTGGAAAAGGGGCCCAGCCTGTCTGTTGACCCAGAAGGTGCACCAAGCACTGCTGCAGGACC
TGGTCTGCCCCATGGAGAAGGATCCACCGCCTGCCGGCGGGACCGTAAACAGGAGCGAGGCCGGTCCCAG
GAGCGGAGGCAGCCCTCATCTTCCTCTTCAGAGAAGCAGCGCTTCTATTCCTGTGACCGCTTTGGGAGCC
GGGAGCCCCCGCAACTGATGCCCTCACTCAGTAGCCACCCCACATCGCCAACAGCGGCGCTAGAGCCAGC
ACCCCACCCACAGGGCAGTGGTTCCGTTAATGGGAGCCCCTTGATGTCAACATCCGGTGCTAGCACCCCG
GGCCGAGGTGGGCGGAGGCAGCTCCCCCAGACTCCTCTGACCCCACGCCCCAGCATCACCTACAAGACCG
CCAATTCCTCGCCTGTCCACTTTGCTGAGGGTCAGAGCGGCCTCCCAGCCTTCTCCCCTGGCCGTCTCAG
CCGCGGCCTTTCTGAACACAATGCCCTGCTCCAGAAAGAGCCCCTGAGCCAGCCTCTAGCTCCTGGCTCC
CGAATTGGCTCTGACCCTTACCTAGGGCAGCGTCTGGACAGTGAGGCCTCCGCCCACACCCTGCCTGAGG
ATACACTCACCTTTGAAGAGGCAGTGGCCACCAACTCTGGCCGCTCCTCCAGGACTTCCTATGTGTCCTC
CCTCACTTCCCAATCCCACCCTCTCCGCCGTGTACCCAATGGCTATCACTGCACTTTGGGACTCAGCACT
GGCGTCCGGGCGCGGCACAGCTACCACCACCCCGATCAGGACCACTGGTGCTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001042528
Insert Size 6984 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001042528.2, NP_001035993.1
RefSeq Size 9772 bp
RefSeq ORF 6984 bp
Locus ID 12287
UniProt ID O55017
Gene Summary Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1B gives rise to N-type calcium currents. N-type calcium channels belong to the 'high-voltage activated' (HVA) group and are specifically blocked by omega-conotoxin-GVIA (AC P01522) (By similarity). They are however insensitive to dihydropyridines (DHP). Calcium channels containing alpha-1B subunit may play a role in directed migration of immature neurons.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...