Cacna1b (NM_001042528) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	alpha(1B); AW050276; AW060892; AW822256; BIII; Cav2.2; Cchn1a
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225275 representing NM_001042528 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCCGCTTCGGGGACGAGCTAGGCGGCCGCTATGGGGGCACCGGCGGCGGGGAGCGGGCTCGGGGTG GCGGGGCCGGCGGGGCGGGTGGCCCGGGCCAGGGGGGTCTGCCGCCGGGCCAGCGGGTCCTGTACAAGCA GTCCATTGCGCAGCGCGCACGGACTATGGCCCTGTACAACCCCATCCCAGTCAAGCAGAACTGCTTCACC GTCAACCGCTCGCTCTTCGTCTTCAGCGAGGACAACGTCGTCCGCAAATACGCTAAGCGCATCACCGAAT GGCCGCCCTTCGAATACATGATCCTGGCCACCATCATCGCCAACTGCATTGTTCTGGCCCTGGAGCAGCA CCTCCCTGATGGGGACAAGACTCCCATGTCTGAGCGACTAGATGACACGGAGCCTTACTTCATCGGGATC TTTTGCTTCGAGGCGGGCATCAAGATCATAGCCCTGGGCTTTGTTTTCCACAAGGGCTCCTACCTTCGGA ACGGCTGGAATGTCATGGACTTCGTGGTGGTCCTCACGGGGATTCTCGCCACAGCTGGAACTGACTTTGA CCTGCGCACACTGAGGGCTGTGCGTGTGCTTAGGCCCCTGAAGCTGGTGTCTGGAATTCCAAGCTTGCAG GTGGTGCTTAAGTCCATCATGAAGGCCATGGTCCCGCTGCTGCAGATTGGGCTGCTGCTCTTCTTTGCCA TCCTCATGTTTGCCATCATCGGCCTCGAATTCTATATGGGCAAATTCCATAAGGCCTGTTTCCCCAACAG CACAGATACAGAGCCTGTGGGTGACTTTCCCTGTGGCAAAGATCCCCCTGCTCGTCAGTGTGATGGTGAC ACCGAATGCCGGGAGTACTGGCCAGGACCCAACTTTGGCATCACCAATTTTGACAACATCCTGTTTGCCA TCTTGACAGTGTTCCAGTGTATCACCATGGAGGGCTGGACTGACATCCTCTACAATACAAATGATGCGGC TGGCAACACATGGAACTGGTTGTACTTCATCCCCCTCATCATCATTGGCTCCTTCTTCATGCTCAACCTG GTGCTGGGTGTGCTTTCCGGAGAGTTTGCCAAGGAGCGGGAGCGAGTCGAGAACCGCCGCGCCTTCCTGA AGCTCCGCAGGCAGCAGCAGATTGAGCGAGAGCTGAATGGGTACTTGGAGTGGATCTTCAAGGCAGAGGA AGTCATGTTGGCAGAGGAGGACAAGAATGCAGAAGAGAAATCCCCTTTGGATGTGTTGAAGAGAGCTGCC ACCAAGAAGAGCCGAAATGACCTCATCCATGCAGAAGAGGGGGAGGACCGGTTTGTAGACCTCTGTGCAG TTGGGTCTCCATTTGCTCGTGCCAGCCTCAAGAGTGGGAAGACGGAGAGCTCATCGTACTTCCGGAGAAA GGAGAAGATGTTCCGGTTCTTTATCCGGCGTATGGTGAAAGCACAGAGCTTCTACTGGGTGGTACTGTGT GTGGTGGCCCTGAACACACTGTGTGTGGCCATGGTGCACTATAATCAGCCTCAGCGGCTTACCACTGCAC TGTACTTTGCAGAGTTTGTTTTCCTGGGTCTCTTCCTCACAGAGATGTCCCTGAAGATGTATGGCCTAGG GCCCAGAAGTTACTTCAGGTCTTCCTTCAACTGCTTTGACTTTGGGGTGATTGTGGGGAGTATCTTTGAA GTAGTCTGGGCTGCCATCAAGCCAGGAACCTCCTTTGGAATCAGTGTGCTGCGGGCTCTGCGACTGCTGA GGATATTCAAAGTTACCAAGTATTGGAACTCTCTGAGGAACCTGGTGGTTTCCCTCCTCAATTCCATGAA GTCCATCATCAGCCTTCTCTTCCTGCTTTTCCTCTTCATCGTGGTCTTCGCTCTGTTGGGGATGCAGCTG TTTGGGGGACAGTTCAACTTTCAAGATGAGACTCCAACCACCAATTTTGATACCTTCCCAGCTGCCATCC TCACTGTCTTTCAGATCCTGACAGGAGAGGATTGGAATGCCGTAATGTATCATGGGATTGAGTCGCAAGG TGGAGTCAGCAAAGGCATGTTTTCTTCCTTTTACTTCATCGTCCTGACACTGTTTGGAAACTACACCCTG CTGAATGTTTTTCTGGCCATTGCTGTGGACAACCTTGCCAATGCCCAGGAGTTGACCAAGGATGAAGAGG AGATGGAAGAAGCAGCCAATCAGAAACTTGCTCTTCAGAAGGCCAAAGAAGTAGCTGAAGTCAGCCCCAT GTCTGCTGCCAATATCTCCATCGCTGCCAGGCAGCAGAACTCGGCCAAGGCGCGCTCAGTATGGGAGCAG CGGGCCAGTCAGCTAAGGCTCCAGAATCTGCGTGCCAGCTGTGAGGCATTGTACAGTGAGATGGACCCTG AGGAGCGCCTGCGTTATGCCAGCACGCGCCATGTGAGGCCAGACATGAAGACACACATGGACCGACCCCT AGTGGTGGAGCCTGGTCGAGATGGCTTGCGGGGACCCGTTGGGAGCAAGTCAAAGCCTGAAGGCACGGAG GCCACAGAAAGCGCGGACCTACCTCGCAGGCACCACCGGCACCGTGATAGGGACAAGACCTCAGCCACAG CACCTGCTGGAGGCGAACAGGACAGGACAGAAAGCACCGAGACCGGGGCCCGGGAGGAACGTGCGCGCCC TCGTCGAAGTCACAGCAAGGAGACTCCAGGGGCTGACACGCAAGTGCGCTGTGAGCGCAGTAGACGTCAC CACCGGCGCGGCTCCCCGGAGGAGGCCACTGAACGGGAGCCTCGGCGCCACCGTGCCCACCGGCATGCAC AGGACTCAAGCAAGGAGGGCACGGCGCCGGTGCTTGTACCCAAGGGTGAGCGACGAGCAAGACACCGAGG CCCACGCACGGGTCCACGTGAGGCAGAGAACAACGAGGAGCCCACACGCAGGCACCGTGCAAGGCATAAG GTGCCACCCACACTGCAGCCCCCAGAGAGGGAGGCTGCAGAGAAGGAGAGCAACGCGGTGGAAGGGGATA AGGAAACCCGAAATCACCAGCCCAAGGAACCTCACTGTGACCTGGAGGCCATTGCAGTTACAGGTGTGGG CCCTCTGCACATGCTGCCCAGCACCTGTCTCCAGAAAGTGGACGAGCAACCAGAGGATGCAGACAACCAG CGTAATGTCACCCGGATGGGCAGTCAGCCCTCAGATCCCAGCACCACTGTGCATGTCCCAGTGACACTGA CAGGCCCTCCTGGGGAGACCCCTGTAGTTCCCAGTGGTAACATGAACCTGGAAGGCCAAGCAGAGGGCAA GAAGGAGGCAGAGGCGGATGATGTGCTGAGAAGAGGCCCCAGGCCCATCGTTCCCTACAGCTCCATGTTT TGTCTCAGCCCCACCAACCTGCTTCGTCGCTTCTGCCATTACATTGTGACCATGCGGTACTTTGAGATGG TAATTCTTGTGGTCATTGCCTTGAGCAGCATTGCCCTGGCTGCAGAGGATCCTGTGCGGACAGATTCATT CAGGAACAACGCTTTAAAGTACATGGATTACATCTTTACAGGAGTCTTCACCTTTGAAATGGTCATAAAG ATGATAGACTTGGGCTTGCTGCTGCACCCTGGTGCCTACTTCCGGGACCTGTGGAACATTCTGGACTTCA TTGTTGTCAGTGGAGCCCTGGTGGCATTTGCGTTCTCGAGCTTCATGGGAGGATCCAAAGGGAAAGACAT CAATACCATCAAGTCTCTGAGAGTCCTGCGTGTCCTGAGGCCCCTCAAGACCATCAAGCGGCTGCCTAAG CTCAAGGCTGTCTTTGACTGTGTGGTGAACTCCCTGAAGAACGTCTTGAACATCCTGATTGTCTACATGC TCTTCATGTTCATATTTGCCGTCATTGCCGTCCAGCTCTTCAAAGGGAAGTTCTTTTACTGTACTGATGA ATCCAAGGAGCTGGAGAGGGACTGCCGGGGTCAGTATTTGGATTATGAGAAGGAAGAAGTAGAAGCCCAG CCAAGGCAGTGGAAGAAATATGACTTCCACTATGACAATGTTCTCTGGGCCTTGTTGACGCTGTTCACAG TGTCCACGGGAGAGGGGTGGCCCATGGTGCTGAAACACTCTGTGGATGCCACCTATGAGGAACAGGGGCC CAGTCCCGGGTTCCGGATGGAGCTCTCCATCTTCTACGTGGTCTACTTTGTGGTCTTCCCTTTTTTCTTT GTCAACATCTTTGTGGCCTTGATCATTATCACCTTCCAGGAACAGGGAGATAAGGTGATGTCTGAATGCA GCTTAGAAAAGAATGAGAGGGCTTGCATTGATTTTGCCATCAGTGCCAAGCCCCTGACACGGTACATGCC TCAAAACAAACAGTCGTTCCAGTATAAGACATGGACATTCGTGGTCTCTCCACCCTTTGAGTACTTCATC ATGGCTATGATAGCCCTCAACACAGTGGTGCTGATGATGAAGTTCTATGATGCACCTTATGAGTACGAGC TGATGCTGAAATGCCTGAACATTGTCTTCACATCCATGTTCTCGATGGAGTGCATACTGAAGATCATCGC CTTTGGGGTATTGAACTACTTCAGAGATGCCTGGAACGTCTTTGACTTTGTCACGGTTTTGGGAAGTATT ACTGATATTTTAGTAACAGAGATAGCGAACAACTTCATCAACCTAAGCTTCCTTCGCCTCTTCCGGGCGG CACGGCTGATCAAGCTGCTTCGCCAGGGCTACACCATCCGCATCCTATTGTGGACCTTCGTCCAGTCCTT TAAGGCGCTGCCCTACGTGTGCCTCCTCATTGCCATGCTGTTCTTCATCTACGCCATCATCGGCATGCAG GTTTTTGGAAACATTGCCCTTGATGATGACACCAGTATCAACCGACACAACAACTTCCGGACATTTCTGC AAGCCTTAATGCTATTGTTCAGGAGTGCCACTGGGGAGGCCTGGCATGAGATCATGCTGTCTTGTCTGGG CAACCGGGCCTGTGACCCACATGCCAACGCCAGTGAGTGCGGGAGCGACTTTGCCTATTTTTATTTTGTC TCCTTCATCTTCCTCTGTTCCTTTCTGATGTTGAACCTCTTTGTTGCTGTAATCATGGACAATTTTGAGT ACCTCACTCGGGACTCTTCCATCCTAGGGCCTCACCACTTAGACGAATTCATTCGAGTCTGGGCTGAATA CGACCCAGCTGCGTGTGGGCGCATCAGTTACAATGACATGTTTGAGATGCTGAAACACATGTCCCCACCT CTGGGGTTGGGGAAGAAATGCCCGGCTCGAGTTGCATACAAGCGCCTGGTTCGCATGAACATGCCCATAT CCAATGAGGACATGACGGTGCACTTTACGTCCACACTGATGGCCCTCATCCGGACAGCACTGGAGATCAA GCTTGCCCCAGCGGGGACGAAGCAGCACCAGTGTGATGCTGAGCTGAGAAAAGAGATCTCTTCTGTGTGG GCTAATCTGCCCCAGAAGACTCTGGACTTACTGGTACCACCCCACAAACCTGACGAGATGACAGTGGGGA AGGTCTATGCTGCTCTCATGATATTTGACTTCTACAAACAGAACAAAACCACCAGAGATCAGACTCACCA AGCTCCCGGAGGCCTGTCCCAGATGGGTCCCGTTTCCCTGTTCCACCCTCTGAAGGCCACCCTGGAACAG ACACAGCCCGCTGTGCTTCGAGGAGCTCGGGTTTTCCTTCGGCAAAAGAGTGCAACTTCCCTCAGCAATG GGGGTGCCATACAAACCCAGGAAAGTGGCATCAAGGAGTCGCTGTCCTGGGGCACGCAGAGGACCCAAGA TGCACTTTATGAGGCCAGAGCACCTCTAGAACGTGGCCATTCTGCAGAGATCCCTGTGGGGCAGTCAGGA ACACTGGCTGTGGATGTCCAGATGCAGAACATGACACTGAGAGGACCAGATGGGGAGCCCCAGCCTGGGC TGGAAAGCCAAGGCAGAGCTGCCTCTATGCCACGCCTAGCGGCAGAAACACAGCCGGCCCCTAATGCCAG CCCCATGAAGCGCTCCATCTCCACACTGGCTCCACGCCCACATGGGACTCAGCTTTGCAGCACAGTTCTG GACCGGCCTCCTCCTAGCCAGGCATCACATCACCACCACCACCGCTGCCACCGGCGCAGAGACAAGAAGC AAAGGTCCCTGGAAAAGGGGCCCAGCCTGTCTGTTGACCCAGAAGGTGCACCAAGCACTGCTGCAGGACC TGGTCTGCCCCATGGAGAAGGATCCACCGCCTGCCGGCGGGACCGTAAACAGGAGCGAGGCCGGTCCCAG GAGCGGAGGCAGCCCTCATCTTCCTCTTCAGAGAAGCAGCGCTTCTATTCCTGTGACCGCTTTGGGAGCC GGGAGCCCCCGCAACTGATGCCCTCACTCAGTAGCCACCCCACATCGCCAACAGCGGCGCTAGAGCCAGC ACCCCACCCACAGGGCAGTGGTTCCGTTAATGGGAGCCCCTTGATGTCAACATCCGGTGCTAGCACCCCG GGCCGAGGTGGGCGGAGGCAGCTCCCCCAGACTCCTCTGACCCCACGCCCCAGCATCACCTACAAGACCG CCAATTCCTCGCCTGTCCACTTTGCTGAGGGTCAGAGCGGCCTCCCAGCCTTCTCCCCTGGCCGTCTCAG CCGCGGCCTTTCTGAACACAATGCCCTGCTCCAGAAAGAGCCCCTGAGCCAGCCTCTAGCTCCTGGCTCC CGAATTGGCTCTGACCCTTACCTAGGGCAGCGTCTGGACAGTGAGGCCTCCGCCCACACCCTGCCTGAGG ATACACTCACCTTTGAAGAGGCAGTGGCCACCAACTCTGGCCGCTCCTCCAGGACTTCCTATGTGTCCTC CCTCACTTCCCAATCCCACCCTCTCCGCCGTGTACCCAATGGCTATCACTGCACTTTGGGACTCAGCACT GGCGTCCGGGCGCGGCACAGCTACCACCACCCCGATCAGGACCACTGGTGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001042528
Insert Size	6984 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001042528.2, NP_001035993.1
RefSeq Size	9772 bp
RefSeq ORF	6984 bp
Locus ID	12287
UniProt ID	O55017
Gene Summary	Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1B gives rise to N-type calcium currents. N-type calcium channels belong to the 'high-voltage activated' (HVA) group and are specifically blocked by omega-conotoxin-GVIA (AC P01522) (By similarity). They are however insensitive to dihydropyridines (DHP). Calcium channels containing alpha-1B subunit may play a role in directed migration of immature neurons.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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