Cacna1e (NM_009782) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	A430040I15; alp; alpha1E; BII; Cach6; Cacnl1a6; Cav2; Cav2.3; Cchr; Cchra1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225252 representing NM_009782 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCGCTTCGGGGAGGCGGTGGTCGTTGGCAGGCCAGGCTCAGGCGATGGAGACTCGGACCAGAGCA GGAACCGACAAGGAACCCCCGTCCCGGCCTCGGGGCCGGCGGCCGCCTACAAGCAGTCAAAAGCGCAGAG GGCGCGGACTATGGCTTTGTACAACCCCATACCAGTCCGGCAGAACTGTTTCACGGTCAACAGATCCCTG TTCATCTTCGGAGAAGATAACATTGTCAGGAAATACGCCAAGAAGCTCATCGATTGGCCGCCATTTGAGT ACATGATCCTGGCCACCATCATTGCCAACTGCATCGTCTTGGCCCTGGAGCAGCATCTTCCTGAGGATGA CAAGACCCCAATGTCTCGAAGACTGGAGAAGACAGAACCATATTTCATTGGGATCTTCTGCTTTGAAGCT GGGATCAAAATTGTGGCTCTAGGGTTCATCTTCCATAAGGGTTCATACCTCCGTAATGGCTGGAATGTCA TGGACTTCATCGTGGTCCTTAGTGGCATCCTGGCCACTGCGGGAACCCACTTCAACACCCACGTGGACCT GCGGACCCTCCGGGCTGTGCGTGTCCTGAGGCCTTTAAAGCTGGTATCAGGAATACCTAGCCTGCAGATC GTGCTGAAGTCTATCATGAAGGCCATGGTGCCTCTTCTGCAGATTGGCCTTCTGCTCTTCTTTGCCATCC TCATGTTTGCTATCATTGGCTTGGAGTTCTACAGTGGCAAGTTGCATCGAGCATGCTTCATGAACAATTC AGGTATTCTAGAGGGGTTTGATCCTCCTCACCCGTGTGGTGTGCAAGGCTGCCCGGCTGGTTATGAATGT AAGGACTGGATCGGCCCCAATGACGGGATCACCCAGTTTGACAACATCCTTTTTGCCGTGCTGACTGTCT TCCAATGCATCACTATGGAAGGGTGGACCACTGTGCTGTACAATACCAATGATGCCTTAGGAGCCACCTG GAACTGGCTGTACTTCATCCCTCTCATCATCATCGGATCCTTCTTTGTTCTCAACCTCGTCCTGGGAGTG CTTTCCGGGGAATTTGCCAAAGAAAGAGAGAGAGTGGAGAACCGAAGAGCGTTCATGAAGCTCCGGCGCC AGCAACAGATTGAGCGAGAATTGAACGGCTACCGTGCCTGGATAGACAAAGCAGAGGAAGTCATGCTTGC TGAAGAAAATAAAAACTCGGGAACATCAGCCTTGGAAGTGCTTCGAAGGGCAACCATCAAAAGGAGCCGG ACGGAGGCCATGACCCGAGACTCCAGCGATGAGCACTGCGTTGATATCTCTTCAGTGGGCACACCTCTTG CCAGAGCCAGTATCAAGAGCACAAAAGTAGATGGGGCCTCCTATTTCCGGCACAAGGAACGGCTTCTGCG CATCTCTATCCGCCACATGGTCAAGTCCCAAGTCTTTTACTGGATCGTCCTGAGTGTTGTGGCTCTTAAT ACTGCCTGTGTGGCCATTGTTCATCACAACCAGCCACAGTGGCTCACTCACCTCCTCTACTATGCAGAGT TTTTGTTTCTGGGACTCTTCCTGTTGGAGATGTCCCTGAAGATGTATGGCATGGGGCCACGCCTCTATTT TCACTCATCCTTCAACTGCTTTGATTTTGGGGTCACAGTGGGCAGCATCTTTGAGGTTGTCTGGGCAATC TTCAGACCTGGCACATCTTTTGGAATCAGTGTCTTACGAGCTCTCCGGCTCCTAAGGATATTTAAAATAA CCAAGTATTGGGCATCCCTCCGGAATCTGGTTGTCTCCTTGATGAGCTCCATGAAGTCTATCATCAGCTT ACTCTTCCTTCTCTTCCTCTTCATCGTTGTCTTTGCTCTCCTGGGAATGCAGCTCTTTGGAGGCAGGTTT AACTTTAATGATGGGACTCCTTCAGCTAATTTTGACACCTTCCCTGCAGCTATCATGACAGTGTTCCAGA TCCTGACAGGGGAAGACTGGAATGAGGTGATGTACAATGGCATTCGCTCCCAGGGTGGGGTCAGCTCAGG CATGTGGTCTGCCATCTACTTCATTGTGCTCACCTTGTTTGGAAACTACACGCTGCTAAATGTATTCTTG GCCATCGCTGTGGACAATCTGGCCAATGCCCAGGAACTGACCAAGGATGAGCAGGAGGAAGAAGAAGCCT TCAACCAGAAACATGCACTGCAGAAGGCCAAGGAGGTCAGCCCGATGTCTGCTCCCAACATGCCTTCCAT TGAAAGAGACAGAAGGAGAAGACACCACATGTCGATGTGGGAGCCACGCAGCAGCCACCTGAGGGAGCGG AGGCGCCGGCACCACATGTCTGTGTGGGAACAACGCACCAGCCAGCTGAGGAGGCACATGCAAATGTCTA GCCAAGAAGCCCTCAACAAAGAGGAGGCCCCGCCAATGAACCCCCTCAATCCACTCAACCCACTGAGTCC TCTCAACCCCCTCAATGCTCACCCCAGCCTTTATCGGAGGCCCAGGCCCATTGAGGGCCTAGCCCTAGGT CTGGGTCTTGAAAAGTGTGAAGAAGAACGCATCAGTCGTGGGGGCTCCCTCAAGGGGGACATAGGGGGCC TGACTAGCGCCCTGGACAACCAGAGGAGCCCCTTGTCCTTGGGCAAACGGGAGCCACCGTGGCTGCCTAG ATCCTGTCATGGAAACTGTGACCCAATCCAGCAGGAGGCTGGGGGAGGAGAGACTGTGGTGACTTTTGAG GACCGGGCCAGGCACAGGCAGAGTCAGAGGCGAAGCCGGCATCGCCGAGTCAGGACTGAGGGCAAGGACT CTGCCTCTGCATCCCGAAGCAGGTCTGCCAGCCAGGAGCGGAGTCTGGATGAAGGGGTGTCCGTTGAGGG GGAGAAGGAACATGAGCCTCACAGCAGCCACAGGAGCAAAGAGCCGACCATCCACGAAGAGGAGAGAACC CAGGACTTAAGGAGGACCAACAGTCTGATGGTCCCCAGGGGCTCTGGACTGGTAGGGGCTCTTGATGAAG CAGAAACACCTCTAGTCCAGCCCCAACCTGAGCTGGAAGTGGGGAAGGATGCAGCTCTGACAGAGCAGGA GGCTGAAGGCAGCAGTGAACAAGCCCTTCTTGGGGACGTACAGCTGGATGTGGGCCGGGGCATTAGCCAG AGTGAACCGGATCTCTCCTGCATGACAGCCAACATGGACAAGGCTACCACGGAGAGCACCAGTGTCACGG TCGCCATCCCTGATGTGGACCCCTTGGTGGACTCGACAGTGGTGAACATTAGCAACAAGACGGATGGAGA AGCCAGTCCCTTGAAGGAGGCAGAGACTAAAGAGGAAGAAGAAGAGGTAGAGAAGAAGAAGAAGCAGAAG AAGGAGAAGCGAGAGACTGGAAAAGCCATGGTGCCCCATAGCTCTATGTTCATCTTCAGCACCACTAACC CGATCCGCAGGGCCTGCCACTACATTGTGAACCTACGCTACTTTGAAATGTGCATTCTCTTGGTGATTGC GGCCAGCAGCATTGCCCTGGCAGCCGAGGACCCTGTACTAACTAACTCGGAGCGTAACAAAGTCCTGAGA TATTTCGACTATGTTTTCACAGGAGTGTTTACCTTTGAGATGGTTATAAAGATGATAGACCAAGGCTTGA TCCTGCAGGATGGATCATACTTCAGAGACCTCTGGAACATCCTGGACTTTGTAGTAGTGGTTGGTGCATT GGTAGCATTTGCTCTGGCGAACGCTTTGGGAACCAACAAGGGGAGGGATATCAAGACTATCAAGTCTCTG AGGGTACTTCGAGTTCTGCGGCCACTGAAAACCATCAAGCGCTTGCCCAAGCTGAAGGCTGTGTTTGACT GCGTGGTGACCTCCTTGAAGAATGTCTTCAACATACTCATTGTCTACAAGCTCTTCATGTTCATCTTTGC CGTCATCGCTGTTCAGCTATTCAAAGGAAAGTTCTTTTATTGCACTGACAGTTCCAAGGACACAGAGAAG GAGTGCATAGGCAACTATGTAGACCACGAGAAAAACAAGATGGAGGTGAAGGGCCGGGAATGGAAGCGTC ATGAATTCCACTATGACAACATCATCTGGGCCCTGCTTACCCTCTTCACAGTCTCTACAGGGGAAGGATG GCCTCAGGTTCTGCAGCACTCTGTAGATGTGACAGAGGAGGACAGAGGCCCAAGTCGCAGCAACCGCATG GAGATGTCCATCTTTTATGTGGTCTACTTTGTGGTCTTCCCTTTCTTCTTTGTCAATATCTTCGTGGCTC TCATTATCATCACCTTCCAGGAGCAAGGGGACAAGATGATGGAGGAGTGTAGCCTGGAGAAGAATGAGCG GGCATGCATCGACTTCGCCATTAGTGCCAAACCTCTCACCCGCTACATGCCACAGAACAGGCACACCTTC CAGTACCGTGTGTGGCACTTTGTAGTCTCCCCATCCTTTGAGTATACCATCATGGCCATGATTGCCTTGA ACACTGTCGTGCTGATGATGAAGTACTACTCTGCTCCTTGCACCTATGAGCTCGCCTTAAAGTACCTGAA CATCGCCTTCACTATGGTGTTTTCCCTGGAGTGTGTCCTAAAGGTCATTGCTTTCGGCTTCCTGAACTAT TTCCGAGATACCTGGAACATCTTTGACTTCATCACGGTGATCGGCAGTATCACAGAAATTATCCTGACAG ATAGCAAGCTGGTGAACACCAGTGGCTTCAATATGAGCTTTCTGAAGCTCTTCCGAGCTGCACGGCTCAT AAAGCTTCTGAGACAGGGCTACACCATCCGCATCTTACTGTGGACCTTCGTGCAGTCCTTTAAGGCTCTT CCCTACGTCTGCCTTCTCATTGCCATGCTTTTCTTCATCTACGCCATCATTGGGATGCAGGTGTTTGGAA ACATAAAGTTAGATGAGGAGAGTCACATCAACCGGCACAACAACTTCCGGAGTTTCTTTGGATCCCTCAT GCTGCTTTTCAGGAGTGCCACGGGGGAGGCTTGGCAGGAGATTATGCTGTCCTGTCTCGGTGAGAAGGGT TGTGAGCCTGACACCACTGCACCCTCAGGGCAGAATGAGAGTGAGCGCTGTGGCACTGACCTGGCCTACG TTTATTTTGTCTCCTTCATCTTCTTCTGCTCCTTCCTGATGCTCAACCTGTTCGTGGCTGTCATCATGGA CAACTTTGAGTACCTGACTCGAGATTCCTCCATCCTGGGGCCTCACCACTTAGATGAGTTTGTCCGTGTC TGGGCAGAATATGACAGAGCAGCATGTGGCCGCATCCATTACACTGAGATGTATGAAATGCTGACTCTCA TGTCACCACCGCTAGGCCTCGGCAAGAGATGTCCCTCCAAAGTGGCCTATAAGAGGCTGGTCCTGATGAA CATGCCAGTGGCTGAGGACATGACAGTCCACTTCACCTCCACACTTATGGCTCTGATCCGGACAGCTCTG GACATTAAAATCGCCAAAGGTGGTGCAGACAGACAGCAGCTAGACTCGGAGCTGCAAAAAGAGACCCTGG CCATTTGGCCTCACCTGTCCCAAAAGATGTTGGATCTGCTTGTGCCCATGCCCAAAGCCTCTGACCTGAC TGTGGGCAAGATCTATGCAGCAATGATGATCATGGACTACTATAAACAGAGTAAGGTAAAGAAACAGAGA CAGCAGCTGGAAGAACAGAAAAATGCACCCATGTTCCAGCGCATGGAGCCCTCATCACTGCCTCAGGAGA TCATTGCTAATGCCAAAGCCCTGCCTTATCTCCAGCAGGACCCTGTTTCTGGCCTGAGTGGTCGGAGTGG ATACCCTTCAATGAGTCCACTCTCCCCTCAAGAAATATTCCAGTTGGCTTGTATGGACCCAGCGGATGAT GGACAATTCCAGGAACAACAATCTCTGGTGGTAACAGACCCTAGCTCTATGCGACGTTCATTTTCTACAA TTCGGGATAAGCGTTCAAATTCCTCCTGGTTGGAGGAGTTCTCCATGGAACGCAGCAGTGAAAACACTTA CAAGTCGCGGCGCAGAAGTTACCATTCCTCCCTGAGGCTGTCAGCACACCGCCTGAATTCTGACTCTGGT CACAAGTCTGACACTCACCGATCAGGAGGCAGGGAGAGAGGCCGATCCAAAGAACGAAAGCACCTCCTCT CTCCAGATGTCTCCCGCTGCAATTCTGAAGAGCGAGGGACCCAGGCCGACTGGGAGTCCCCAGAACGTCG CCAGTCCAGGTCACCCAGTGAAGGAAGGTCACAGACCCCCAATAGACAGGGTACCGGTTCCCTAAGTGAG AGCTCCATCCCCTCTATTTCCGACACCAGCACCCCAAGACGAAGCCGTCGCCAGCTCCCACCTGTGCCAC CAAAGCCTCGTCCCCTCCTCTCCTACAGCTCCCTGATGAGACACACTGGCGGCATCTCTCCACCTCCTGA TGGAAGTGAGGGTGGATCCCCACTGGCCTCTCAAGCTCTGGAGAGCAACAGCGCTTGCCTGACCGAGTCT TCCAACTCCTTGCACCCCCAGCAGGGCCAGCATCCTTCCCCACAGCACTACATCTCCGAGCCCTATCTGG CCCTCCATGAAGACTCCCACGCCTCAGACTGTGGCGAGGAGGAGACACTCACCTTTGAGGCAGCCGTAGC CACTAGCTTGGGCCGGTCCAATACCATTGGCTCTGCGCCACCCCTACGGCACAGCTGGCAGATGCCTAAT GGGCACTATCGGCGGCGGAGGCGTGGGGGGCCTGGGCCTGGCATGATGTGTGGAGCTGTCAGTGACCTCC TGAGTGACACGGAAGAAGACGATAAGTGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_009782
Insert Size	6822 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_009782.3, NP_033912.2
RefSeq Size	12697 bp
RefSeq ORF	6822 bp
Locus ID	12290
Gene Summary	This gene encodes an integral membrane protein that belongs to the calcium channel alpha-1 subunits family. Voltage-sensitive calcium channels mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes. Voltage-dependent calcium channels are multi-subunit complexes, comprised of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The isoform alpha-1E gives rise to R-type calcium currents and belongs to the high-voltage activated group. Calcium channels containing the alpha-1E subunit may be involved in the modulation of neuronal firing patterns, an important component of information processing. [provided by RefSeq, Jul 2008]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.