Brwd1 (NM_001103179) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	5330419I02Rik; D530019K20Rik; G1-403-16; repro5; Wdr9
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225248 representing NM_001103179 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGCCTTCGCCGGCCCGACGTCCGGTGCCTCTCATCGAGTCGGAGCTGTACTTTCTCATCGCCC GCTACCTCTCAGCTGGCCCGTGTCGCCGAGCGGCGCAGGTGCTGGTGCAGGAGCTGGAGCAGTACCAGCT GTTGCCGAAGAGGTTGGACTGGGAGGGCAACGAGCACAACAGGAGCTACGAGGAATTGGTCTTGTCCAAT AAACATGTGGCTCCTGACCACCTACTACAGATATGCCAGCGCATCGGTCCGATGCTGGATAAGGAGGTCC CGCCCAGTATCTCAAGAGTCACTTCTTTGCTTGGTGCAGGAAGGCAGTCTTTGCTACGAACAGCAAAAGA CTGCAGGCACACAGTGTGGAAGGGCTCTGCCTTTGCTGCTCTTCACAGAGGAAGACCTCCTGAAATGCCC GTCAATTATGGCCCCCCACCGAGTCTTGTGGAGATACACCGAGGAAGGCAGCTCACAGGGTGTTCCACTT TTAGCACAGCGTTCCCAGGAACTATGTACCAGCATATAAAGATGCACAGGAGGATTCTTGGACACCTGTC TGCAGTGTACTGTGTAGCATTTGATAGGACAGGACATAGAATCTTTACAGGTTCAGATGATTGCTTGGTA AAGATTTGGTCAACACATAATGGCCGCTTGTTGTCTACGTTAAGAGGCCATTCTGCAGAGATTTCAGACA TGGCTGTGAACTACGAGAACACGCTGATTGCTGCAGGAAGCTGTGATAAGATCATTAGGGTGTGGTGCTT GAGGACCTGCGCCCCAGTTGCTGTGCTGCAAGGACACACGGGGTCAATCACATCTCTGCAGTTTAGCCCG ATGGCCAAAGGACCTCAGCGGTACATGGTTTCTACGGGCGCTGATGGCACTGTTTGCTTCTGGCAGTGGG ATTTAGAGTCCTTGAAGTTCAGTCCACGACCCCTGAAGTTCACGGAAAAGCCAAGGCCTGGTGTTCAGAT GCTGTGTTCTTCATTTAGCGTTGGTGGTATGTTTTTAGCTACAGGTAGTACTGATCATGTAATCAGAATG TATTTTTTGGGATTCGACGCACCTGAAAAAATCGCCGAGCTTGAAAGCCACACTGACAAAGTTGATAGTA TCCAGTTTTGTAACAATGGTGACCGGTTCTTAAGTGGTAGCCGAGATGGAACAGCACGGATCTGGAGGTT TGAGCAGTTGGAATGGAGAAGCATTTTACTGGACATGTCTGCCAGGATCTCGGGAGACACATCTTCAGAA GAAGAGAGGTTTATGAAACCCAAAGTTACAATGATTGCTTGGAACCAGGATGATAGCACCGTGGTCACAG CCGTGAATGATCATGTCCTCAAGGTGTGGAGTTCCTACACTGGACAGCTGCTTCATAACTTACTGGGACA TGCTGATGAAGTGTTTGTTTTGGAGACACATCCCTTTGATTCCAGAATTATGTTATCTGCAGGACACGAT GGCAGCATATTTATATGGGATATTACAAAGGGCATCAAGATGAAGCATTACTTTAATATGATCGAAGGAC AAGGACATGGAGCTGTGTTTGACTGCAAGTTTTCACAGGATGGACAACATTTTGCCTGTACAGATTCTCA CGGGCATCTTCTGATATTTGGCTTTGGATGCAGCAAGCCATATGAAAAGATTCCTGATCAGATGTTTTTT CATACTGACTACCGACCACTGATAAGAGATTCCAATAACTATGTCTTGGATGAGCAGACACAGCAGGCAC CTCATCTTATGCCTCCACCATTCTTGGTGGATGTGGACGGAAACCCTCATCCAACCAAGTTCCAGAGATT AGTACCAGGTCGAGAAAACTCAGCAGATGAACATTTGGTTCCACAGCTAGGCTATGTGGCCACAAGTGAT GGGGAGGTGATTGAACAGATTATAAGCCTGCATACCAACGACAGTGGAGATGCAAGCCCAGAGTCCAGCG TCTTGGATGGCATGATTAGGCAGCTGCAGCAGCAGCAAGACCAGAGACTGGGAGTGGATCAGGATGGGAC TGCAAATGGACTTCCCAGTGGGGAAGGGACACCCCGAAGAGGGAGTTTTAGAAGACTGAGTTTAGACATT CAGTCTCCTCCAAACATCGGTCTCCGTCGGAGTGGGCAGGTTGAAGGTGTTCGTCAGATGCATCAGAACG CTCCTCGGAGTCAGATTGCTACAGAGCGTGACCTCCAGGCTTGGAAGCGGAGAGTAGTTGTTCCAGAGGC ACCGCCTGGCATGTTCAGGCGGTTGGAAGACATTCGCATAGAGAGAGGCGAGGAGGAGAGGAACCTGTAT GTAATAGGGAGAAAGAAGAAGACTTTCCAGGTCCCACAAAAGTCCAATTCGATGGTTTTGGTGTCCCAGT CTAGACAACGGACGTGTAGGCGTAAATATGCAAATTATAGTAGAAGAAACGCTGACCAGTGTGAATTATC TTCTGGAAATGAATCTTCAGGCTCTGTTAGACATGAGACATCTTATGATCAGAGTGAAGGTTCTTGTTCT TCAGAAGATGACGAGTGGAGAAATGACAGAAGAAGTGGTAGTGACAGCGACAGCTCAAGTGACTCTTCAT CTCAGTATTCTGATTGGACAGCTGACACGGGGATCAACTTGCAGCCTCCTCTCAGGATGTCATGTCGTCG TCGAGTAACTCGATTCTGCAGTACTTCAGAGGACGAAATGTCCATGGAGAACATATCACCTCCTAAACGA AGGCGGAAAAGAAGGAAAGAAAGCAAGCCTAAGAGGGAGAGTTTACGAAGGATGACTCCAGCAGAACTTG CAAATATGGAACACCTATATGAGTTTCACCCTCCTGTGTGGATAACGGACACCACACTTCGGAAGTCTCC CTTTGTTCCTCAGATGGGTGATGAGGTGATGTACTTCCGTCAGGGTCATGAAGCTTATATTGAAGCTGTC AGAAGAAATAACATTTATGAACTAAATCCTCACAAGGAGCCATGGAGGAAAATGGATCTTAGGGATCAAG AACTGGTCAAAATTGTTGGACTAAGGTATGAGGTTGGACCACCTACACTTTGTTGCCTCAAACTAGCATT TATAGATCCAGCGACTGGCAGACTGACGGATAAATCTTTCTCTATTAGATATCATGACATGCCAGATGTT ATTGATTTTCTTGTGTTGCGCCAATTTTATGATGAAGCAAGACAGAGAAACTGGCAGCCTTGTGACAGAT TTCGATCTATAATTGATGATGCTTGGTGGTTTGGAACCGTGTTGAGTCAAGAACCGTATCAACCCCAGTA TCCTGACAGTCACTTCCAGTGTTACATTGTTAGGTGGGACAACACCGAGACTGAAAAGCTTAGCCCTTGG GACATGGAACCGATTCCTGACAATGTTGATCCTCCAGAAGAATTGGGAGCAAGTATCTCTGTCACTTCAG ATGAGCTAGAGAAATTGCTCTACAAACCACAAGAAGGAGAATGGGGCCAGAGGTCAAGAGATGAGGAGTG TGATCGGATCATCAGTGGCATAGACCAGCTTTTAAACCTCGATATAGCAGCAGCGTTTGCAGGCCCGGTG GATCTGTGCACATATCCCAAGTACTGCACAGTAGTGGCTTATCCAACTGATCTCTACACAATTCGAATGA GGCTTGTTAATCGATTTTACAGGCGGTTATCTGCATTAATTTGGGAAGTCCGATATATAGAGCATAATGC AAGAACGTTTAATGAACCGGAAAGTGTAATTGCAAGATCAGCTAAAAAAATAACTGACCAGCTTCTGAAG TTTATCAAGAATCAAGATTGTACAAATATATCGGAACTATGTAACACTTCTGACAATGATGAGGACGACA GTGCTGATGATTTGGATGATAGTGATCTTCCTAAAACGTCTTCTGGAAGAAGGAAGGTCCACAACTGGAA AAGGAGGAGTAGAGCCAGTGGCTGTATTGAGAGTGACTGGAGGAGGCAGTGCAAGGCCCTGCTTATCCTC ATCTTCCAGTGTGAGGACTCGGAGCCCTTTAGACAGCCCGTGGACTTGGATGAGTACCCAGATTACCGAG ATATTATAGATACGCCAATGGATTTTGGAACAGTAAGGGAAACTCTAGAAGCTGGGAATTACGATAGCCC TGTGGAGTTTTGCAAAGACATTCGATTGATATTTAGCAATGCAAAGGCATATACACCGAACAAAAGATCA AAGATTTATAGTATGACCTTGAGATTGTCTGCTTTGTTTGAAGAAAAAATGAAGAAAATCTCCTTTGATT TTAAAATTGGTCAAAAATTCAAGGAGAAACTTCGTAGAAGCCAAAGATTCAAGCAACGACAAAACTGTAA CGGTGCTGTCCCTGGTGACAGGCGCAGGAGAAATGTCAAGCAGAAGCAGTTCAAATCTCAGACAAAAGTA ATTCCACAGTTGATGTGTCCTCCTTCTCAGTCTACCTCAAGTAAGGTACCTTTGTCTGCAACCCGCAAGA CAAGTGCTGGTGTCTCTTCAGGCTTTACTTCTGGGGACTCTTCAGACTCTGCAGGATCTTTAGAAAGAGT CAGAAGACAGAGGCCTGAAGTACTCAGGAGTGGTTCTGTGCTGTTTGGAAGTGAAATGGAAGATTTCTTA GCTACCTCTTCATCTTCTTCAGCTTCAAACAGTTCAGAGGAAAGCAAAGCGAGTCCTGGAGCTCGAGAAT CTTCCTTGCGCAGTGGTGTGTTGAGAGGCAGCAACCTCGGGGTGACCAGAACAAGAGCTGCTCGGAGGAA AGCTGGTTCTGTTTCTTTAGAAAATGGGTGTGGTAGAAAAGCCACTCGGAAGAGAGTCTATTTAAGTGAT TCTGATAACAATTCAGTGGAAACAGATGAAAATCTGAAAAATAGAAAATGTGGAAGTAGCCGGAAAGTTC TGCGGAAGTGTGCTGCTGTTGCTGCCAGCAAGATAAAGCTGATGAGTGATGCAGAGGATTCGAGCTCAGA GAGCCCCTGCTCTGGGCGGAAGCTGCCACATCGAAACGCTTCAGCTGTGGCCAGGAAGAAGTTGCTACAC AACTCTGATGATCAGAGCCTTAAGTCAGAGACAGAAGAACTGAAAGACCAGAACCAATCACTGTTGATAT CCGGTCCCCATTCTGTCCACAACAGCATCAGTGACTCAGAGTCAGACAGTGACTTGCGAGCAACCCGGAA GACCTGGAATGCTAACGGCTGCACATCCCACACTGCAGCAACATGCAAAACCAAGTCACGTCCAATAGAG TCTTCTGAGGAAGATTCCAGATGTCATGGATCTGATCATGGGCCCAGCAGCACTGGGGACCCTTCAACAT CTGGGCAGAAACTCAGGGCAGATAGCATCTCTGAGGAAGCTGACTCTGAACCAGAAAGCAGTGTACTATG CAAGAATACACATCTGTGTAAGAAAGCCAAGATCTTGAGTGACTCAGAAGACTGTGAGGAAAAGTGTGGA GAAAGGAGAGGTCCTGAAGTGGAAGGAAGCCCAGTTTCTGAAGCCTTGAGGGAAGCCATTCTTGCCCCCC AGTGTCTCTCACATCGTGGATCTGAGACTGATGTGGATTCTGACGGCGGTGCAGTGAGAGAGAAATCATA CAGCAATGAAAACGGATCTGTATCACTAGAGAATGGACAAAGAAGAAAAGTTTCCCGAAAAAGTTCCAGT GACAAGGAAAGTAATCTACAGGTAACCCAGAAGTCACCCAAAGACAGAAGCAGCCCCTCGAGGATTACTC AGCGTGCTAGTGTGGCTACTGATAAAATGAAGCTCACAAGTGATGCAGAAGACCTCAGCCTAGAGAGCGT GTGTACTCGAAGCAAAAGGAGAAGGAAAAAGCCTGCTCGATTTGCTTGTACCCCAGCAAAGACTGCATTG AGTAGTGAGGAGAAACACGCACATTGTGAAGTGCCTGAGGCACAGCCTGCCTGCAGGAACAAGCTCCCTG AGCCTGAACACCAAGATAGTGCAGAGAATCCCAGTCAGGCTGCAAGTGCAGACTTGAATTCTGGAGGGGG TTCCAGTTTTGAACAAAGAAAAAGCATACAGAGCAGGCAAATGGGCGCTGTCTGCGTAAGGCCACCTCCT AAAACCCAAAGCTCCTCTGCTGGGCTTTCTCAGGAAAACGCCAGAAGCCAAACCCTAGATAGTGAGACTA GCTTGCCTTCTGAGTCGGTTTTGACCCAGAAAGCTACTGTAGAGAGTAACTTTGAAGAAGAACTGAACTA TGGATTAAGAAGGTGGAATGGCAGAAGGCTCAGGACATACGGGAAGGCCCCACTCAGTAGGACAGCACAA GTTACTCCCAGTTTACAGGCCTCAGCAGAGGTCGGGGTGAAGAGGAGGAGAATGCATCCTGAGGTAGATG GTGAGGACGTGCCCGGACAAATGGGGAGTTCAGGATGCGGACCTGACACCAGTCCCAAGGCATCAGACTT GGGGTCAGTAACTGACTCTGATGTTGACTGTACCGATAACACACAAACCCAAAGGAAGAAGAAAAGGAAA GGGAAAGCAAGAGTGCTTAGCAAAGGTAAACCCTCCTCATCCAACTCACCTAAAACTGCAAGACATCCAA GGCAGAGTAAAGGTCCTAGGTTAAATGTGGATGACAATGACTGGGAGGATTTGGACTATGCAAAAGCTAA AGGAGTTGTCCGGCTCTCAAAAATCCAAACGAGAAATCAGGGTAGGAGAACGGTGAGATACCACGATGGG GAAGATGATAGAAGCATAGAGAGCGCACTAGAGCTCACTGACCGCACCTTACGCCCTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001103179
Insert Size	6780 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001103179.1, NP_001096649.1
RefSeq Size	7547 bp
RefSeq ORF	6780 bp
Locus ID	93871
UniProt ID	Q921C3
Gene Summary	May be a transcriptional activator. May be involved in chromatin remodeling. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 3' UTR and CDS compared to variant 1. The resulting isoform (B) is shorter and has a unique C-terminus compared to isoform A.

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