Med12 (NM_021521) Mouse Untagged Clone
CAT#: MC225218
Med12 (untagged) - Mouse mediator of RNA polymerase II transcription, subunit 12 homolog (yeast) (Med12), (10ug)
CNY 35,250.00
货期*
14周
规格
Product images
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经常一起买 (3)
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | 230kDa; Mopa; OPA-1; Tnrc11; Trap230 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC225218 representing NM_021521
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCTTTCGGGATCTTGAGCTATGAACACCGACCCCTGAAGCGGCTGCGGCTGGGGCCTCCCGATG TGTACCCTCAAGATCCCAAACAGAAGGAGGATGAACTGACGGCTTTGAATGTAAAACAAGGTTTCAATAA CCAGCCTGCTGTCTCTGGGGACGAACATGGCAGTGCCAAGAACGTCAACTTCAATCCTGCCAAGATCAGT TCCAACTTCAGCAGCATCATCGCAGAGAAGTTAAGGTGTAATACTCTCTCTGACACTGGTCGCAGGAAGT CCCTAATGAACCAGAAGGACAACTTCTGGCTGGTGACTGCAAGATCTCAGAGTGCTATTAACACCTGGTT CACTGACCTGGCTGGCACCAAACCACTCACACACCTAGCCAAAAAGGTCCCCATTTTCAGTAAAAAGGAA GAAGTATTTGGGTATTTGGCCAAATACACGGTGCCTGTGATGCGGGCTGCCTGGCTCATTAAGATGACTT GTGCCTACTATGCAGCAATGTCTGAGACTAAGGTTAAGAAGAAAAATACTGCTGACCCCTTCACTGAATG GACTCAAATCATCACAAAGTACTTGTGGGAGCAGCTACAGAAGATGGCTGAGTACTATCGGCCAGGGCCT GCAGGAAGTGGAGGCTGTGGTTCTACTATAGGGCCTTTGCCCCATGATGTAGAGATGGCCATCAGGCAGT GGGATTACAATGAGAAGCTCGCCTTGTTCATGTTTCAGGATGGAATGCTGGACAGACATGAGTTCCTGAC TTGGGTGCTTGAGTGTTTTGAGAAAATACGCCCTGGAGAAGATGAATTGCTTAAATTGCTGCTTCCCCTA CTGCTGCGATACTCAGGGGAGTTTGTTCAGTCTGCCTATCTGTCCCGCCGCCTTGCCTACTTCTGTACCC GGAGATTGGCTCTCCAGCTGGATGGTGTGAGCAGCCACTCATCTCATGTCATAGCTGCTCAGTCAACAAG TTCTCTGCCCACTACCCCAGCACCTCAGCCCCCAACTAGCAGTACACCCTCGACTCCCTTTAGTGACCTG CTTATGTGCCCTCAGCATCGTCCCCTGGTTTTTGGCCTCAGCTGTATCCTTCAGACCATCCTTCTGTGTT GCCCTAGTGCCCTAGTTTGGCACTACTCATTGACTGATAGCCGAATTAAGACCGGCTCACCACTTGACCA CCTGCCTATTGCTCCTTCCAACTTGCCCATGCCAGAGGGTAACAGTGCCTTTACTCAGCAGGTCCGTGCA AAATTACGAGAGATCGAACAACAGATCAAGGAGCGTGGACAAGCAGTTGAGGTTCGCTGGTCTTTTGATA AGTGCCAGGAAGCTACTGCAGGTTTCACCATTGGACGGGTGCTCCATACTTTAGAAGTGCTGGATAGCCA TAGTTTCGAGCGCTCTGACTTTAGTAACTCTCTTGACTCCCTTTGTAATCGAATCTTTGGATTGGGGCCT AGTAAGGATGGTCATGAGATTTCCTCTGATGATGATGCTGTGGTATCATTATTGTGTGAATGGGCTGTAA GCTGCAAACGCTCAGGTCGGCATCGTGCTATGGTAGTAGCCAAACTCCTAGAGAAGAGACAAGCAGAAAT TGAGGCTGAGCGCTGCGGAGAATCTGAAGCAGCTGATGAGAAGGGCTCCGTCGCCTCTGGTTCCCTTTCC GCTCCTAGTGCACCCATTTTCCAGGATGTCCTCCTGCAGTTTCTGGATACACAGGCTCCCATGCTGACTG ATCCCCGAAGTGAAAGTGAGCGAGTAGAATTCTTTAACTTGGTACTGCTCTTCTGTGAACTGATCCGACA TGATGTCTTCTCCCATAATATGTACACCTGCACTCTCATCTCTCGGGGGGATCTTGCATTTGGAGCCCCT GGTCCTCGGCCTCCCTCTCCCTTTGATGATCCTACAGATGATCCAGAGCGCAAGGAGGCTGAAGGCAGCA GCAGCAGCAAGCTAGAGGACCCAGGGCTCTCTGAATCTATGGACATCGACCCTAGTTCCAGTGTGCTTTT TGAGGACATGGAAAAGCCTGATTTCTCATTGTTCTCCCCTACTATGCCTTGTGAGGGGAAGGGAAGCCCA TCCCCTGAGAAACCAGATGTCGAAAAGGAAGTAAAACCCCCAGCCAAAGAGAAGATCGAGGGGACACTTG GGATTCTGTATGACCAGCCACGACATGTGCAGTATGCCACACACTTTCCAATCCCACAGGAGGAGTCATG CAGCCATGAGTGCAACCAGCGGTTGGTCGTACTGTTTGGGGTGGGGAAGCAGCGAGATGATGCCCGCCAT GCCATCAAGAAGATTACCAAGGATATCCTGAAGGTTCTGAATCGCAAGGGGACAGCAGAAACTGACCAGC TTGCTCCTATTGTGCCTCTGAATCCTGGAGACCTGACATTCTTAGGTGGGGAAGATGGGCAGAAGCGACG ACGCAACCGGCCCGAAGCCTTCCCCACTGCTGAAGATATTTTTGCTAAGTTCCAGCACCTTTCCCATTAT GACCAACACCAGGTCACGGCTCAGGTCTCCCGGAACGTTCTGGAGCAGATCACGAGCTTCGCCCTTGGCA TGTCGTACCACTTGCCTCTGGTGCAGCATGTGCAGTTCATCTTCGACCTCATGGAATATTCACTGAGCAT CAGTGGCCTCATCGACTTTGCCATCCAGTTACTGAATGAGCTGAGTGTGGTTGAGGCGGAGCTCCTTCTC AAATCGTCGGATCTGGTGGGCAGCTACACTACCAGCCTGTGCTTATGTATCGTGGCTGTCCTTCGACACT ATCATGCCTGCCTCATCCTCAATCAGGACCAGATGGCGCAAGTGTTTGAGGGGCTCTGTGGCGTGGTGAA ACATGGAATGAACCGTTCAGATGGCTCCTCTGCAGAGCGCTGTATCCTTGCTTATCTCTATGATCTGTAT ACCTCCTGTAGCCATTTAAAGAGCAAATTTGGGGAGCTCTTCAGTGACTTTTGCTCAAAAGTGAAGAACA CCATCTACTGTAACGTGGAGCCATCGGAATCCAATATGCGCTGGGCACCGGAGTTCATGATTGACACTCT GGAGAACCCTGCCGCTCACACTTTCACCTACACGGGGCTCGGCAAGAGTCTTAGTGAGAACCCTGCTAAC CGCTATAGCTTTGTGTGCAATGCTCTTATGCACGTCTGCGTGGGCCACCATGATCCTGATAGGGTAAATG ACATCGCCATCCTTTGTGCAGAGCTGACCGGCTATTGCAAGTCCTTGAGTGCTGAGTGGCTAGGAGTACT TAAGGCCTTGTGCTGCTCCTCGAACAATGGCACTTGTGGTTTCAATGATCTCCTGTGCAACGTAGACGTC AGTGATTTGTCTTTTCATGATTCCTTGGCTACTTTTGTTGCTATCCTCATCGCCCGTCAATGTTTGCTCC TAGAAGACCTGATTCGCTGTGCAGCCATCCCTTCACTCCTCAATGCTGCTTGTAGTGAGCAGGATTCTGA GCCAGGAGCCAGGCTTACTTGCCGAATCCTCCTCCACCTGTTCAAGACGCCACAACTCAATCCTTGCCAG TCTGATGGAAACAAACCTACTGTTGGAATCCGGTCCTCCTGTGACCGCCACCTGCTGGCTGCCTCCCAGA ACCGCATCGTGGATGGGGCTGTGTTTGCTGTTCTCAAGGCTGTGTTTGTACTCGGAGATGCGGAGCTAAA AGGTTCAGGCTTTACTGTGCCGGGAGGAACAGAAGAACTTCCAGAAGAGGAGGGAGGAGGTGGTAGTAGC GGTCGGAGACAGGGTGGCCGCAACATCTCTGTGGAGACAGCAAGTCTGGATGTCTATGCCAAGTACGTGC TGCGAAGCATCTGCCAACAGGAATGGGTAGGAGAACGTTGCCTTAAGTCACTGTGTGAGGATAGCAATGA TCTACAAGACCCAGTGTTGAGTAGTGCCCAGGCCCAGCGCCTCATGCAACTTATCTGCTACCCACATCGA CTGCTGGACAATGAGGATGGAGAAAACCCCCAGCGGCAGCGCATTAAACGTATTCTCAAGAATTTAGACC AATGGACCATGCGCCAGTCCTCTTTGGAGCTACAGTTGATGATCAAGCAGACCCCCAACACTGAGATGAA CTCTCTCTTGGAGAACATTGCCAAGGCCACAATCGAGGTTTTCCAACAGTCAGCAGAGACGGGGTCATCT TCTGGAAGTACAGCAAGCAACATGCCCAGCAGCAGCAAGACCAAACCTGTGCTCAGCTCTCTAGAGCGAT CTGGTGTATGGTTGGTGGCTCCTCTCATTGCCAAACTGCCCACTTCAGTCCAGGGCCATGTATTAAAAGC TGCTGGGGAGGAACTGGAAAAGGGTCAGCACCTGGGCTCTTCTTCCCGTAAAGAACGAGATCGACAGAAA CAGAAGAGCATGTCCCTGTTGAGCCAACAGCCTTTCTTATCACTGGTGCTAACATGTCTGAAAGGACAGG ATGAGCAGCGCGAGGGACTCCTGGCCTCCCTCCACAGCCAGGTGCACCAGATTGTGATTAATTGGCGAGA AAACCAGTACTTAGATGATTGCAAACCAAAGCAGCTAATGCATGAGGCACTCAAACTGCGGCTCAACCTG GTGGGGGGCATGTTTGACACTGTGCAGCGTAGTACCCAGCAGACTACAGAGTGGGCCCAGCTTCTCCTTG AGATCATCATCAGCGGCACTGTGGACATGCAGTCTAACAATGAGCTCTTCACTACTGTGTTGGACATGCT GAGCGTGCTTATCAACGGAACGTTGGCTGCAGACATGTCCAGTATCTCGCAAGGCAGCATGGAGGAAAAC AAGCGTGCATATATGAACCTGGTGAAGAAGCTTCAGAAGGACTTGGGGGAGCGCCAATCAGACAGTCTGG AGAAGGTTCACCAACTGTTGCCACTACCCAAGCAGAACCGAGATGTCATAACCTGTGAGCCACAGGGCTC CCTTATTGACACCAAGGGCAACAAGATTGCTGGCTTCGATTCCATCTTCAAGAAGGAGGGTCTACAAGTT TCTACCAAACAAAAGATCTCTCCCTGGGAGCTTTTTGAGGGCCTGAAGCCATCAACAGCACCACTGTCAT GGGCCTGGTTTGGCACAGTCCGAGTGGACCGCAGAGTGGCACGAGGGGAGGAGCAGCAGCGGCTGTTGCT CTATCATACCCACCTGAGGCCTCGACCCAGAGCCTATTACCTGGAACCACTACCTCTGCCCCCAGAAGAT GAGGAGCCACCAGCCCCTGCCCTACTAGAGCCTGAGAAAAAGGCTCCTGAGCCCCCCAAGACTGACAAAC CAGGGGCTGCTCCTCCGAGCACTGAGGAGCGCAAAAAGAAGTCTACCAAGGGCAAAAAACGCAGCCAGCC AGCCACCAAGAACGAGGACTATGGCATGGGGCCAGGTCGGAGTGGCCCCTATGGTGTGACAGTGCCTCCA GACCTTCTACACCATGCAAATCCTGGTTCTATATCCCACCTTAGCTACAGGCAAAGCTCCATGGGCCTGT ATACCCAAAACCAGCCACTACCTGCTGGTGGCCCTCGTGTGGATCCATACCGCCCCGTGCGATTACCAAT GCAAAAGCTGCCAACTCGACCAACTTATCCCGGTGTGCTGCCTACAACTATGTCTACTGTCATGGGCCTA GAACCCTCTTCTTATAAGACATCTGTATACCGGCAGCAGCAACCCACAGTGCCCCAGGGACAGCGCCTTC GCCAACAGCTCCAGGCAAAGATACAGAGTCAGGGGATGTTGGGACAGTCATCTGTCCATCAGATGACCCC TAGTTCTTCCTATGGTTTGCAGACTTCCCAGGGCTATACATCCTATGTTTCTCATGTGGGATTGCAGCAA CACACAGGCCCTGCAGGTACCATGGTGCCCCCCAGCTACTCCAGCCAGCCTTATCAGAGCACCCACCCTT CTACCAATCCTACTCTTGTAGATCCTACCCGCCACCTGCAACAGCGGCCCAGTGGCTATGTGCATCAGCA GGCCCCAACCTATGGGCATGGACTGACTTCCACTCAAAGGTTTTCACACCAGACACTGCAGCAGACACCC ATGATGGGTACCATGACTCCGTTGAGTGCCCAGGGTGTCCAGGCAGGCGTCCGTTCAACTTCCATCCTGC CTGAGCAGCAGCAACAACAACAGCAGCAGCAGCAACAGCAGCAGCAGCAACAGCAGCAGCAGCAACAACA ACAGCAGCAGCAGCAGCAGCAACAACAACAGCAGTACCATATCCGACAGCAACAGCAGCAGCAGCAGATG CTACGGCAACAGCAGCAACAACAGCAACAGCAGCAGCAGCAGCAACAGCAGCAGCAGCAACAACAACAGC AACAGCAGCAGCAGCAGCCACACCAGCAGCAGCAGCAGGCAGCTCCTCCCCAACCCCAGCCCCAGTCCCA GCCCCAGTTCCAGCGCCAGGGCCTGCAGCAGACCCAGCAGCAGCAACAGACAGCAGCTTTGGTCCGGCAA CTTCAACAACAGCTCTCTAATACCCAGCCACAGCCCAGCACCAACATATTTGGACGCTACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_021521 |
Insert Size | 6573 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_021521.2, NP_067496.2 |
RefSeq Size | 8464 bp |
RefSeq ORF | 6573 bp |
Locus ID | 59024 |
UniProt ID | A2AGH6 |
Gene Summary | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway (By similarity).[UniProtKB/Swiss-Prot Function] |
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