F5 (NM_007976) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI173222; Cf-5; Cf5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225213 representing NM_007976 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCCTAGTCTGCCCGTGCTTCTTCCTCCTGGTGGTTCTGGGAACCCGCTGGGCGGGCTGGGGCAGCC ACCAGGCAGAGGCCGCGCAACTAAGGCAGTTCTATGTGGCAGCTCAGGGGATCCTCTGGAACTATCATCC TGAGCCCACAGATCCAAGTTTGAATTCTATACCTTCCTTCAAGAAAATTGTCTACAGAGAGTATGAACAG TATTTTAAGAAAGAAAAGCCACGATCTAGCAACTCAGGACTTCTTGGACCTACTTTATACGCTGAAGTTG GGGACGTCATTAAAGTTCACTTTAGAAACAAAGCAGACAAACCACTAAGCATCCATCCTCAAGGGATTAA ATACAGTAAATTTTCAGAAGGGGCTTCTTACGCAGACCACACGTTCCCTGCCGAGAGGAAGGATGATGCC GTGGCTCCTGGAGAAGAATACACCTATGAATGGATCGTCAGTGAGGACAGCGGGCCCACACCTGATGACC CACCATGCCTCACCCACATCTACTATTCCTATGAAAACCTGACCCAGGATTTCAACTCGGGTCTGATTGG GCCTCTGCTTATCTGCAAGAAAGGCACCCTGACCGAGGATGGGACTCAGAAGATGTTTGACAAGCAGCAT GTGCTCCTATTTGCTGTGTTTGATGAAAGCAAGAGCCGGAGCCAGTCACCATCCCTAATGTACACAATTA ATGGCTTTGTGAATAAGACGATGCCAGATATAACAGTCTGTGCCCATGACCACGTCAGCTGGCATCTGAT CGGGATGAGCTCGGGGCCAGAATTGTTTTCTATTCACTTCAACGGCCAAGTCCTAGAGCAGAACCAGCAT AAAGTGTCCACCGTCACCCTGGTCAGCGCAACATCTACGACTGCAAACATGACTATGAGCCCAGAAGGAA GATGGATTGTTTCTTCTCTCATCCCAAAGCATTATCAAGCTGGGATGCAGGCTTACATTGACATTAAAAA CTGCCCAAAGAAAACGAGGAGCCCCAAGACCCTCACTCGGGAGCAGAGGCGGTACATGAAGAGATGGGAG TATTTCATAGCCGCAGAGGAGGTCATTTGGAACTATGCACCCGTGATACCTGCGAATATGGACAAAATTT ACAGGTCTCAGCACTTGGATAATTTCTCAAACCAAATTGGAAAACATTACAAGAAAGTTATCTACAGGCA ATATGAAGAAGAGACCTTCACCAAACGCACTGACAACCCCAGCATCAAACAAAGTGGGATTCTGGGCCCT GTTATCAGAGCCCAGGTCAGAGACACACTCAAGATCGTGTTCAAAAATATGGCGAGCCGACCCTACAGCA TTTACCCTCACGGGGTGACCTTCTCTCCTTACGAAGATGGAATCAATTCTTCCTCCACCTCAGGCAGTCA CACCACGATCAGACCAGTTCAACCGGGGGAAACCTTCACTTACAAATGGAACATTCTAGAGTTTGATGAA CCCACGGAAAACGATGCCCAGTGCCTAACAAGGCCATACTACAGTGATGTGGACGTTACAAGGGATATTG CCTCTGGGCTGATAGGGCTGCTTCTAATTTGTAAGAGCAGGTCCCTGGACCAGAGGGGTGTACAGAGGGT GGCAGACATCGAGCAGCAGGCCGTGTTTGCTGTGTTTGACGAGAACAAGAGCTGGTACATTGAGGACAAC ATCAACAAGTTCTGTGAGAATCCTGATGAGGTGAAGCGTGATGATCCCAAGTTTTACGAATCAAACATCA TGAGCACTATCAACGGCTACGTGCCCGAGAGCATTTCCACTCTGGGATTCTGTTTTGATGACACTGTCCA GTGGCACTTCTGCAGTGTGGGAACTCATGATGATATTTTGACCATCCACTTCACTGGGCACTCGTTCATC TATGGGAGGAGGCACGAGGACACCTTGACCCTGTTCCCCATGCGTGGTGAATCTGTGACAGTTACAATGG ATAATGTTGGAACTTGGATGTTGACCACCATGAATTCCAATCCAAAACGCAGAAACCTAAGACTGAGATT CAGAGATGTTAAGTGTAATCGGGATTATGACAATGAGGACTCATATGAGATTTATGAACCTCCTGCACCT ACATCCATGACAACTCGGAGAATTCATGATTCCTTAGAAAATGAATTTGGCATAGACAACGAAGATGATG ATTACCAGTACTTACTGGCGTCATCATTAGGAATTAGGTCATTCAAAAACTCATCATTGAATCCAGAGGA AAATGAGTTCAATCTCACTGCTCTCGCTCTGGAGAACAGCTCTGAGTTCATATCTCCAAGCACAGACAGA GTTGTTGACTCAAACTCTTCACGAATCCTTAGTAAAATCATCAATAATAACCTCAAAGACTTTCAAAGAA CACTTCCTGGCTCAGGAGCCACCGTGGCTGGTACCCTCCTTAGAAACCTCATTGGCTTAGATGAGAACTT CGTCCTCAACTCTTCTACAGAACATCGTTCCAGCTCATATCATGAAAATGATATGGAAAATCCACAGTCA AACATCACAATGGTATACCTACTTCCTCTTGGTCCAAAAGGATCTGGGAATCGAGAACAAGATAAACCTA AAACCATCAAGACAGGAAGACCCCACATGATGAAGCACAGGTTCTCCTGGATGAAAGCGCCAGCTGGTAA AACTGGGAGGCATTCAAACCCAAAGAATTCGTATTCTGGAATGAAGTCTGAGGAGGACATTCCTAGCGAG TTGATACCCTTAAAGCAAAAGATCACTTCCAAATTTCTGAATAGACGATGGCGTGTGGCTTCTGAAAAGG GTAGTTATGAAATAATAGCAGCAAATGGTGAAGACACAGATGTGGATAAGCTGACCAACAGTCCTCAAAA TCAGAATATCACAGTACCTCGGGGAGAGAGCACCTCTCACACAAACACAACAAGAAAGCCAAGTGACCTC CCAACATTTTCTGGAGTTGGACATAAATCTCCACATGTAAGACAGGAGGAAGAAAACAGTGGTTTTCAGA AAAGACAGTTATTCATCAGGACACGGAAGAAGAAGAAAAATAAGAAGCTTGCACTACACAGTCCTCTATC TCCAAGGGGCTTTGACCCTTTGAGAGGACATAACCATTCCCCATTTCCAGACAGGAGACTACTTAATCAC TCACTGTTACTCCACAAGTCCAATGAAACAGCTCTTTCTCCAGACCTGAACCAGACCTCTCCTTCAATGA GTACGGACAGGTCACTTCCTGACTATAATCAGTACTCGAAAAATGACACTGAGCAGATGAGCTCTTCTTT AGATCTTTATCAGTCAGTGCCCGCAGAGGAACACTCTCCAACATTTCCTGCCCAAGATCCTGATCAAACA CACTCTACCACAGATCCTAGCTACAGATCCTCTCCGCCAGAGCTCAGCCAGGGGCTTGATTATGACCTAA GTCATGACTTTTACCCTGATGACATTGGTCTAACATCTTTCTTTCCAGACCAAAGTCAAAAGTCATCTTT CTCTTCAGATGATGACCAAGCAATCCCTTCCTCAGACTTAAGCCTCTTTACCATCTCTCCAGAATTGGAT CAGACAATTATTTACCCAGACCTGGATCAGTTGCTCCTTTCTCCAGAAGACAATCAGAAGACCTCCTCCC CAGACCTGGGCCAGGTGCCCCTTTCTCCAGATGACAACCAGAAGACCTCCTCCCCAGACCTGGGTCAGGT GTCCCTTTCTCCAGATGATAACCAGAAGACCTCCTCCCCAGACCTGGGTCAGGTGCCCCTTTCTCTAGAT GACAACCAGAAGACGACCTCCCCAGACCTGGGTCAGGTGCCCCTTTCTCCAGATGACAACCAGATGATCA CCTCCCCAGACCTGGGTCAGGTGCCCCTTTCTTCTGATAACCAGAAGACCTCTTCCCCAGATCTGGGTCA GGTGCCTCTTTTTCCTGAAGACAACCAGAATTACTTCCTAGACCTGAGTCAGGTACCTCTCTCCTCAGAC CAAAACCAGGAGACCTCCTCCACAGACCTACTGACTCTCTCTCCTGATTTTGGTCAGACAGTCCTTTCCC CAGACTTGGATCAGCTGCCACTCCCTTCAGACAATAGTCAGGTGACCGTTTCCCCAGACCTCAGCCTCTT GACCCTCTCACCAGATTTTAATGAGATAATCCTAGCCCCAGACCTTGGTCAAGTGACCCTCTCTCCAGAC CTCATCCAGACAAACCCTGCTCTTAATCATGGACACAAAGCATCCTCTGCAGACCCTGATCAAGCATCCT ACCCTCCAGATTCTGGTCAGGCTTCATCGCTTCCAGAACTGAATCGGACTCTTCCTCATCCAGATCTCAC TCACATACCACCTCCTTCACCATCTCCCACACTCAATAACACTTCTTTGTCAAGGAAATTTAACCCTCTT GTTGTAGTAGGTCTCAGTAGAGTAGATGGAGACGACGTTGAGATTGTTCCAAGTGAGGAGCCAGAGAGAA TAGATGAAGATTATGCCGAGGATGACTTTGTAACCTATAATGACCCCTACAGAACAGACACTAGGACAGA TGTCAATTCCTCCAGAAATCCTGACACTATCGCAGCATGGTACCTCCGAGGCCACGGTGGACACAAAAAA TTCTACTATATTGCAGCTGAAGAAATAACCTGGAATTACGCAGAGTTTGCACAAAGTGAAATGGACCATG AAGACACAGGCCACACTCCAAAGGACACCACATACAAGAAAGTCGTTTTCAGAAAATACCTTGATAGCAC GTTTACAAGTCGTGATCCTCGGGCAGAATATGAGGAGCACCTTGGCATTCTCGGTCCTGTGATCCGGGCT GAAGTGGATGATGTGATCCAAGTTCGATTTAAAAATTTGGCATCCAGACCGTATTCTCTTCATGCTCACG GACTTTCCTATGAAAAATCCTCAGAGGGGAAGACTTATGAAGATGAATCTCCTGAATGGTTTCAGGAAGA TGATGCTGTCCAGCCCAATAGCAGTTACACCTATGTATGGCATGCCACCAAGCGCTCAGGGCCAGAGAAC CCTGGTTCTGCCTGCCGGGCTTGGGCCTACTATTCTGCAGTGAATGTGGAGAGGGACATCCACTCAGGCT TGATCGGCCCCCTTCTGATCTGCCGGAAAGGAACACTTCACATGGAGCGCAACCTGCCTATGGACATGAG AGAGTTTGTCTTACTCTTCATGGTCTTTGATGAGAAGAAGAGCTGGTACTATGAAAAGTCCAAGGGGTCA CGGAGAATTGAATCCCCAGAAGAGAAAAATGCCCACAAGTTTTACGCAATTAATGGGATGATCTACAACC TGCCCGGCCTGAGAATGTACGAGCAAGAGTGGGTGAGGCTACACCTGCTGAACATGGGCGGCTCCCGAGA TATTCACGTGGTTCACTTCCATGGCCAGACCCTGCTGGATAATAGGACCAAACAGCACCAGTTAGGCGTC TGGCCCCTTCTGCCTGGTTCATTTAAAACTCTTGAAATGAAGGCATCCAAGCCTGGCTGGTGGCTCCTAG ACACAGAGGTTGGAGAAAACCAGGTAGCTGGCATGCAAACGCCATTTCTCATCATAGACAAAGAGTGTAA GATGCCAATGGGACTAAGCACTGGTGTCATATCTGATTCACAGATCAAGGCTTCGGAATATCTGACTTAT TGGGAGCCCAGATTAGCACGATTAAACAATGCTGGTTCATACAATGCTTGGAGTATAGAAAAAACTGCAT TAGATTTTCCCATTAAACCTTGGATCCAGGTGGACATGCAGAAGGAAGTTGTAGTCACCGGGATACAAAC CCAAGGTGCTAAACACTACCTAAAGTCCTGCTTTACCACGGAGTTCCAAGTGGCTTACAGCTCTGACCAA ACCAACTGGCAGATCTTCAGAGGGAAGAGCGGGAAGAGCGTGATGTATTTTACTGGTAATTCAGATGGCT CTACAATAAAAGAGAATCGACTTGACCCACCCATTGTGGCTAGATACATTAGGATACACCCAACAAAATC CTATAATAGACCCACCCTTCGGCTGGAGCTGCAGGGCTGTGAGGTGAACGGATGTTCCACACCACTGGGC CTGGAAGATGGACGGATTCAAGACAAGCAAATTACTGCATCTTCATTTAAAAAGTCGTGGTGGGGAGACT ACTGGGAGCCCTCCCTTGCCCGCCTGAACGCCCAGGGCCGCGTGAACGCCTGGCAAGCCAAGGCAAACAA CAACAAGCAGTGGTTACAAGTCGATCTGCTCAAAATCAAGAAGGTAACGGCCATCGTAACGCAGGGCTGT AAGTCTCTGTCCTCTGAGATGTACGTGAAGAGCTACAGCATCCAGTACAGTGACCAGGGTGTGGCATGGA AACCTTACCGACAGAAATCCTCCATGGTGGACAAGATTTTTGAAGGAAACAGCAATACCAAGGGGCACAT GAAGAACTTTTTCAACCCGCCCATTATTTCCAGATTTATCCGCATCATTCCTAAAACATGGAACCAGAGC ATCGCCCTTCGCCTAGAGCTCTTCGGCTGTGACATTTATTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_007976
Insert Size	6552 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_007976.3, NP_032002.1
RefSeq Size	7433 bp
RefSeq ORF	6552 bp
Locus ID	14067
UniProt ID	O88783
Gene Summary	This gene encodes a glycoprotein coagulation factor that plays a critical role in the process of blood coagulation and hemostasis. The encoded protein is activated by thrombin, to generate a heterodimer containing heavy and light chains held together by calcium ions. About half of the mice lacking the encoded protein die at an embryonic stage possible due to abnormal yolk-sac vasculature while the remaining animals succumbed to massive hemorrhage immediately after birth. A point mutation in this gene has been shown to cause disseminated intravascular thrombosis in the perinatal period, resulting in frequent deaths of newborn mice. [provided by RefSeq, Apr 2015]

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