Cacna1d (NM_028981) Mouse Untagged Clone

CAT#: MC225205

Cacna1d (untagged) - Mouse calcium channel, voltage-dependent, L type, alpha 1D subunit (Cacna1d), transcript variant 1, (10ug)



  "NM_028981" in other vectors (2)

CNY 34,770.00


货期*
12周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 8430418G19Rik; C79217; Cach3; Cacn; Cacn4; Cacnl1a2; Cav1.3; Cchl; Cchl1; Cchl1a; Cchl1a2; D-LTC; D-LTCC
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC225205 representing NM_028981
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACCTTCCGACATTTTCTAGTGATCTAATTTTAATTAAAAGCGTTCTCTCCCAAGAGACTGATGCCC
GATATAAAGGCAGAGTTGTAAGTGCGGTAGAAAGCACGGAGGACTTCTCCCAGGCCTTTGCAGAGGCAAA
CTATGCAAGAGGCACCAGACTCCCTATTTCTGGTGAAGGACCAACTTCTCAGCCCAACAGCTCCAAGCAA
ACTGTCCTATCTTGGCAAGCTGCAATCGATGCTGCTAGACAGGCCAAGGCCGCCCAGACCATGAGCACCT
CTGCACCCCCACCTGTAGGATCTCTCTCCCAAAGAAAACGTCAGCAATACGCCAAGAGCAAAAAACAGGG
TAACTCGTCTAACAGCCGACCTGCCCGTGCCCTTTTCTGTTTATCCCTCAATAACCCCATCCGAAGAGCC
TGCATTAGTATAGTGGAATGGAAACCATTTGACATATTTATATTATTGGCTATTTTTGCCAATTGTGTGG
CCTTAGCTATTTACATCCCATTCCCTGAAGATGATTCTAATTCAACAAATCATAACTTGGAAAAAGTAGA
ATATGCCTTCCTGATTATTTTTACAGTGGAGACATTTCTGAAGATTATAGCGTATGGACTGTTGCTGCAT
CCTAATGCTTACGTTAGGAATGGATGGAATTTACTGGATTTTGTTATAGTAATTGTAGGATTGTTTAGTG
TAATTCTGGAACAATTAACCAAAGAAACAGAAGGCGGGAACCACTCTAGTGGCAAATCAGGAGGCTTTGA
TGTCAAAGCCCTCCGTGCCTTCCGAGTGTTAAGACCACTTCGACTTGTATCAGGAGTGCCCAGTTTACAA
GTTGTTCTGAACTCCATTATAAAAGCCATGGTCCCCCTCCTTCACATAGCCCTTTTGGTATTATTTGTAA
TTATCATCTATGCTATTATAGGATTGGAACTTTTTATTGGAAAAATGCACAAAACATGTTTTTTTGCTGA
CTCAGATATAGTAGCTGAAGAGGACCCAGCTCCGTGTGCGTTCTCAGGGAATGGACGCCAGTGCACCGCC
AATGGCACGGAATGTAGGAGTGGCTGGGTTGGCCCCAATGGAGGCATCACTAACTTTGATAACTTTGCCT
TTGCCATGCTCACTGTGTTCCAGTGCATTACCATGGAGGGCTGGACAGATGTGCTCTACTGGGTTAATGA
TGCGATAGGATGGGAATGGCCATGGGTGTATTTTGTTAGTCTGATCATCCTTGGCTCATTTTTCGTCCTT
AACCTGGTTCTTGGTGTCCTTAGTGGAGAATTCTCCAAGGAAAGAGAGAAGGCTAAAGCTCGTGGAGATT
TCCAGAAGCTTCGGGAAAAACAGCAGCTGGAAGAGGACCTCAAGGGCTACCTGGACTGGATCACCCAGGC
AGAAGACATCGATCCCGAGAACGAGGAGGAGGGTGGCGAGGAAGGCAAACGAAACACTAGCATGCCCACC
AGCGAGACTGAATCTGTCAACACAGAGAACGTAAGTGGTGAAGGCGAGACCCAGGGATGCTGTGGAACTC
TCTGTCAGGCCATCTCGAAATCCAAACTCAGCCGTCGATGGCGTCGCTGGAACCGGTTCAATCGCAGGAG
GTGCAGGGCTGCCGTGAAGTCTGTCACGTTTTACTGGCTGGTGATTGTCCTGGTGTTTCTCAACACTTTA
ACTATTTCCTCTGAGCATTACAATCAGCCAGACTGGCTGACACAGATTCAAGACATCGCAAACAAAGTCC
TCTTGGCTCTGTTCACCTGTGAGATGCTGGTGAAGATGTACAGCCTGGGCCTCCAGGCTTACTTTGTCTC
CCTCTTCAACCGCTTCGATTGCTTTGTGGTGTGTGGGGGCATCACCGAAACCATCCTGGTGGAACTGGAG
CTCATGTCTCCCCTGGGGGTCTCCGTGTTCCGGTGTGTGCGCCTCCTGAGAATCTTCAAAGTGACCAGGC
ACTGGACCTCCCTGAGCAACTTGGTGGCATCCTTGTTAAACTCCATGAAGTCCATCGCCTCTCTGCTGTT
ACTCCTCTTCCTCTTCATCATCATCTTCTCCTTGCTGGGGATGCAGTTGTTTGGTGGGAAGTTTAATTTT
GACGAGACTCAAACCAAGCGAAGCACCTTTGACAACTTTCCGCAAGCACTCCTGACGGTGTTCCAGATCC
TGACAGGTGAAGACTGGAATGCGGTGATGTATGACGGCATCATGGCCTATGGAGGCCCTTCCTCTTCCGG
GATGATTGTCTGTATCTACTTCATCATCCTTTTCATCTGTGGTAACTATATCCTGCTGAATGTCTTCTTG
GCCATCGCTGTGGACAATTTGGCTGATGCTGAAAGTCTGAACACTGCTCAGAAAGAGGAAGCTGAAGAAA
AAGAAAGGAAAAAGATTGCCAGAAAAGAAAGCCTAGAAAACAAAAAGAACAACAAACCAGAAGTCAACCA
GATAGCCAACAGTGACAACAAGGTTACAATTGATGATTATCAGGAAGATGCTGAAGACAAGGACCCTTAC
CCGCCCTGTGATGTGCCAGTAGGTGAAGAGGAAGAGGAGGAGGAAGAGGATGAGCCCGAAGTTCCTGCTG
GTCCCCGCCCTCGCAGAATCTCAGAGTTGAACATGAAGGAGAAGATTGCGCCCATCCCAGAAGGAAGCGC
CTTCTTCATTCTTAGCAAGACCAACCCGATACGTGTGGGTTGCCACAAGCTCATTAACCACCACATCTTC
ACCAACCTTATCCTGGTCTTCATCATGCTGAGCAGCGCTGCCCTGGCTGCAGAGGACCCCATCCGTAGCC
ACTCATTCCGCAACACGATACTGGGCTACTTTGACTATGCCTTCACAGCCATCTTTACGGTTGAAATCCT
GTTAAAGATGACAACTTTTGGAGCCTTCTTGCACAAAGGGGCTTTCTGCAGGAACTACTTCAATTTGCTG
GACATGCTGGTTGTTGGGGTGTCGCTGGTGTCCTTTGGGATTCAGTCCAGCGCCATCTCCGTTGTGAAGA
TTCTGAGGGTTTTGAGGGTCTTGCGGCCTCTCAGAGCAATCAACAGAGCAAAGGGACTTAAGCACGTGGT
CCAGTGTGTCTTTGTGGCCATCCGAACCATTGGCAACATCATGATCGTCACGACCCTGCTCCAGTTCATG
TTTGCATGCATTGGGGTCCAGCTGTTCAAGGGGAAGTTCTACCGTTGCACAGATGAAGCCAAAAGTAACC
CTGAGGAGTGCAGGGGGCTTTTCATTCTTTATAAGGACGGCGATGTTGACAGTCCTGTGGTCCGTGAGAG
GATCTGGCAAAATAGTGATTTCAATTTTGACAATGTCCTTTCGGCTATGATGGCGCTGTTCACAGTCTCA
ACTTTTGAGGGCTGGCCCGCGTTGCTGTACAAAGCTATTGATTCAAACGGTGAGAACGTTGGTCCTGTCT
ACAACTACCGTGTGGAGATCTCCATCTTCTTCATCATCTACATCATCATCGTTGCCTTCTTCATGATGAA
CATCTTTGTGGGCTTCGTCATCGTCACCTTCCAGGAACAGGGAGAAAAAGAGTATAAGAACTGTGAGCTG
GACAAAAATCAGCGTCAGTGTGTTGAATATGCCTTGAAAGCACGCCCCTTAAGGAGATACATCCCCAAAA
ACCCATACCAGTACAAGTTCTGGTATGTGGTGAACTCCTCGCCTTTCGAATACATGATGTTCGTCCTCAT
CATGCTCAACACGCTCTGCCTGGCCATGCAGCACTATGAGCAGTCCAAGATGTTCAATGATGCCATGGAC
ATTCTGAACATGGTCTTCACAGGGGTCTTCACTGTTGAGATGGTTTTGAAAGTCATTGCTTTCAAGCCCA
AGGGGTATTTTAGTGACGCCTGGAACACGTTTGACTCCCTCATCGTAATCGGCAGCATTATAGACGTGGC
CCTCAGCGAAGCCGACAACTCTGAAGAGAGCAATAGAATCTCCATCACCTTTTTCCGTCTTTTCCGAGTG
ATGCGGTTGGTGAAACTTCTCAGCAGGGGGGAAGGCATCCGGACACTGCTGTGGACCTTCATCAAGTCCT
TTCAGGCGCTGCCGTATGTTGCCCTCCTCATTGCCATGCTCTTCTTCATCTACGCTGTCATAGGCATGCA
GATGTTTGGGAAAGTTGCCATGAGAGATAACAACCAGATCAATAGGAACAACAACTTCCAGACCTTTCCT
CAGGCAGTGCTCCTACTCTTCAGGTGTGCAACAGGGGAAGCCTGGCAGGAGATCATGCTTGCCTGCCTCC
CAGGAAAGCTGTGCGACCCAGACTCCGATTACAACCCAGGAGAGGAGTACACTTGTGGGAGCAACTTTGC
CATTGTCTACTTCATCAGCTTTTACATGCTCTGTGCATTCCTGATCATCAACCTCTTTGTGGCTGTCATC
ATGGACAATTTTGACTATCTGACTCGGGACTGGTCTATTCTGGGGCCTCACCACTTGGACGAATTCAAAA
GAATATGGTCAGAATATGACCCTGAAGCAAAGGGAAGGATAAAACACCTTGATGTGGTCACTCTGCTCCG
GCGGATCCAGCCTCCCCTGGGGTTTGGAAAATTATGCCCACACCGAGTAGCATGTAAGAGATTGGTTGCC
ATGAACATGCCTCTCAACAGTGATGGGACAGTCATGTTCAATGCAACCCTGTTTGCTTTGGTCCGGACAG
CTCTCAAGATCAAGACTGAAGGGAACCTGGAGCAAGCTAATGAAGAACTCCGAGCTGTGATCAAGAAAAT
CTGGAAGAAGACAAGCATGAAGCTGCTTGACCAAGTTGTCCCTCCAGCTGGTGATGATGAGGTAACCGTG
GGGAAGTTCTATGCCACTTTCCTGATACAGGACTACTTTAGGAAATTCAAGAAACGGAAAGAGCAAGGAC
TGGTGGGGAAGTACCCTGCGAAGAACACCACGATTGCCCTACAGGCGGGATTAAGGACCCTGCATGACAT
TGGGCCAGAAATCCGACGGGCTATATCCTGTGACTTGCAAGATGACGAGCCAGAAGACTCCAAACCAGAA
GAAGAAGATGTATTCAAAAGAAATGGTGCCCTGCTTGGAAACCATGTCAATCATGTTAATAGTGATAGGA
GAGATTCCCTTCAGCAGACCAATACCACCCACCGTCCCCTGCATGTCCAAAGGCCTTCAATGCCACCTGC
AAGTGATACTGAGAAACCGCTGTTTCCTCCAGCAGGAAATTCGGGGTGTCATAACCATCATAACCATAAT
TCCATAGGGAAGCAAGCTCCCACCTCAACAAATGCCAATCTCAATAATGCCAATATGTCCAAAGCTGCCC
ACGGAAAGCCGCCCAGCATTGGGAACCTTGAGCATGTGTCTGAAAATGGGCATTACTCCTGCAAGCATGA
TCGAGAGCTTCAAAGAAGGTCCAGTATTAAAAGAACCCGCTATTACGAGACTTACATTAGGTCTGAGTCA
GGAGATGAGCAATTTCCAACCATTTGCCGTGAAGATCCTGAGATACACGGCTACTTCCGAGACCCCCGCT
GCTTGGGGGAACAGGAGTATTTCAGTAGCGAGGAGTGCTGTGAGGACGACAGCTCTCCCACCTGGAGCAG
GCAAAACTACAACTACTACAACAGGTACCCAGGCAGCTCCATGGACTTTGAGAGGCCCCGAGGCTACCAC
CACCCCCAAGGCTTCTTAGAAGATGATGACTCTCCCACTGGCTATGATTCACGGAGATCTCCGAGGAGAC
GTCTTCTACCTCCTACCCCACCATCCCATCGGAGGTCCTCCTTCAACTTTGAGTGTCTGCGCAGGCAAAG
CAGCCAAGACGATGTCCTTCCATCGCCTGCCCTGCCTCACCGCGCTGCCCTGCCCCTGCACCTGATGCAG
CAGCAGATCATGGCCGTTGCAGGCCTAGATTCCAGTAAAGCCCAGAAGTACTCACCGAGCCACTCTACCC
GGTCGTGGGCCACCCCTCCAGCAACCCCTCCATACCGAGACTGGAGCCCATGCTACACCCCTTTGATCCA
GGTGGACCGCTCAGAATCTATGGACCAGGTCAATGGCAGCCTGCCATCCCTACACCGAAGCTCCTGGTAC
ACAGATGAACCCGACATCTCCTATAGGACTTTCACGCCAGCTAGTCTGACTGTCCCCAGCAGCTTCCGGA
ACAAGAACAGCGACAAGCAGAGGAGTGCAGACAGCCTGGTGGAGGCAGTCCTGATATCCGAAGGCTTGGG
ACGCTACGCGAGGGACCCCAAATTTGTCTCAGCGACAAAACATGAGATTGCCGATGCCTGTGACCTAACC
ATAGACGAGATGGAAAGTGCTGCCAGCACCCTGCTCAATGGCAGTGTGTGTCCTCGAGCCAATGGGGACA
TGGGCCCCATCTCCCACCGCCAGGACTATGAGCTCCAGGACTTTGGTCCTGGCTACAGTGACGAGGAGCC
AGACCCTGGGCGAGAGGAAGAGGACCTGGCAGATGAGATGATTTGCATTACCACCTTGTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_028981
Insert Size 6501 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_028981.3, NP_083257.2
RefSeq Size 8701 bp
RefSeq ORF 6501 bp
Locus ID 12289
UniProt ID Q99246
Gene Summary This gene encodes a pore-forming subunit of the L-type, voltage-activated calcium channel family. These channels have been found to play a role in heart and smooth muscle contraction and in the transmission of auditory information. Homozygous knockout mice for this gene exhibit deafness and heart defects. These channels have also been linked to mitochondrial oxidative stress in a mouse model of Parkinson's disease. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2014]
Transcript Variant: This variant (1) differs in the 5' UTR and has multiple coding region differences, compared to variant 3. These differences cause translation initiation at an alternate start codon, compared to variant 3. This results in a distinct N-terminus and shorter protein (isoform 1), compared to isoform 3.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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