Nin (NM_001081453) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	3110068G20Rik; AI385615; AU024711; mKIAA1565
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225174 representing NM_001081453 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGAGGTGGAGGAGGACCAGCATGAAGCCCGACTCAAGGAGCTGTTTGACAGTTTTGACACCCTGG GAACTGGGTCTCTGGGTCAAGAGGAGCTCACTGACCTCTGCCACGTGCTGTGCTTGGAGGATGTGGGCCC GGTGCTGCAACAGACGCTACTCCAGGACAACCTCTTGGGCAGGGTACATTTTGACCAATTTAAAGAAGCA TTAATACTTATCTTGTCTAGAACTCTATCCAGTGAGGAGCACTTTGAGGAGTCAGACTGTTCCCCAGAAG CTCAGCCCAAATATGTTAGAGGTGGGAAGCGTTATGGACGAAGATCACTGCCCGAGTTTCAAGAGTCTGG GGAAGAGATCGAGGAGGTGACAGTGCTTGAGCCACTAGAGGAAGAAGCGAGGTCATCACCCATCCCAGCT GGGGACTGCGGCGAGCACTGGAAGACACAACGCAGTGAGGAGTATGAAGCAGAAGGCCAGCTGAGGTTTT GGAACCCAGATGACCTGAATGCTTCACATGGTGGGTCTTGCCCTCCTCCAGACTGGATAGAAGAGAAACT GCAAGAGGTTTGTGAAGATCTGGGGATCACTCGAGATGGTCACCTGAACCGGAAGAAGCTGGTTTCCATC TGCGAACAGTATGGATTACAGAATGTGGATGGAGCGATGCTGGAAGAGGTGTTTCTCAGCCTTGATCCTG ATGGAACAATGAGTGTGGAAGACTTTTTCTATGGTCTGTTTAAGACTGGGAAATCCCTCACGCCATCAGC ATCTACTCCCTATAGACAACTGAAACGGCATCTCTCTATGCAGTCTTTCGATGAGAGTGGACGGCGGACC GCAACCTCGTCAGCAATGACGAGTACCATTGGCTTCCGGGTCTTCTCCTGCTTGGATGACGGGATGGGCC AGGCGTCTGTGGAGAGGATACTGGACACCTGGCAGGAAGAAGGCATTGAGAACAGTCAGGAGATTCTGAA GGCCCTGGATTTCAGCCTGGATGGAAACATCAACCTGACAGAGCTGACGCTGGCTCTGGAGAATGAACTT TTGGTTACCAAGAATGGCATTCACCAGGCAGCACTAGCTAGCTTCAAGGCCGAGATCCGGCACTTGTTAG AACGAGTTGACCAAGTAGTCAGAGAAAAGGAGAAGCTACGGTCGGACCTGGACAAAGCAGAAAAGCTGAA GTCTCTCATGGCTTCCGAGGTGGACGATCACCACGCGGCCATTGAGCGGAGGAATGAATACAACCTCAGG AAACTGGATGAAGAATACAAGGAGCGCATAGCAGCCTTAAAGAACGAACTCCGCCAGGAGAGGGAACAGA TGTTGCAGCAAGTGGGCAAGCAGCGGGTGGAGCTGGAACAGGAAATTCAAAAGGCCAAGACGGAAGAGAA TTACATCCGGGACCGCCTTGCCCTCTCCCTGAAGGAAAACAATCGCCTGGAGACTGAGCTTCTGGAAAAC GCAGAGAAGTTGGCAGAGTACGAGAGCCTGACGCAGAAGCTCCAGCGCAGTTTGGAAAATGTGTTAGCAG AAAAGTTTGGTGACCTCGATCCCAGCAGTGCTGAGTTCTTTCTTCAAGAGGAGAGGCTGGCGCAGATGAG AAATGAATATGAGCAACAGTGCAGGCTATTACAAGACCAAGTGGATGAGCTTCAGTCTGAACTAGAAGAA TACCAGGCTCAAGGCAGGGTGCTCAGACTCCCCTTGAAGAACTCGCTGTCAGAAGAACTCGACGGTCACA GTGGTGGTATTGAGCCCGACCAAGGGCCTGGATCTGAAGAGTGCAACCCCCTGAATATGAGCATTGAGGC AGAGCTGGTCATCGAGCAGATGAAGGAGCAGCACCACCGGGACCTGTGTCACCTGAGACTGGAGCTGGAA GACAAAGTGCGCCACTATGAAAAGCAGCTGGATGATACCAGGGTCGCCTCTGAACAGGAGCAGGCAGCCA TGAAGCAGAAGTATGAGCAGGGAGTGCACACCTTGGAGAAGCGAGTCAGCGAGCTTCGGAGTGAGATTGC AGACCTGGAAGGGCAGGCAGCCGTGCTGAGGGAGGCCCACCACAAGGCCAGCTGCAGGCACGAGGAGGAA AAGAGACAACTGCAAATGGCGTTCGATGAGGAAAAGGCTCAGCTCCAGGAGGAGCTGCGGCAGGAGCATG AGAGGGAGCTCCAGGCCAGGCTGCAGCAGGCAGCGGAGAGCTTTCGCCAGGAGAGAGAGGGACTCGCCCA GGCTGCCTGGACTGAGGAGAAGGTGAGAGGCTTGGAGCAATCTTACCAGGAGCAGCTGCTGAGCCTGGAG GAGAAGCATGCTCTAGAGAAAGAAGAGCTGAGGGAAGAGCTCTCGGAACATCACCGAAGGGAACTCCAGG AGGGAAGGGAAGAAATGGAAACAGAGTGTAATAGAAGAGTCTCTCAGATAGAAGCCCAGTGCCAGGCTGA CTGTGAGAAAGTCACTGAGCACTGTGAACAAACTCTGCAAAGCCTCGAGGTTCGCCACCGCCAGGAGCTG AGGGACCTCTTGGACCAGCACCTGGAGGAAAGGTCTCAGTGGGAGTTTGAGAAGGATGAGCTTACACAAG AGTGCACAGATGCCCAGGAGCAGCTGAAGGAGGCGCTCCAGAGGGAGAGGGCGACCGCTGCGGCCATGAA GCAGGAACAGGAGATACTGGAGAGGACATACAAGGACCGTTTGAATATCCTGAGCACGGAAAGAAAGCAG CTGCTGCAAGACCTGAAAGACCTACAGAACGCATCTGAGAGCCAGCATGGCCTCCTGTCTGGCCAGATAC TTGAGCTGAAGAGAAGTCAGGAGAGAGAACTGAGGGACCAAGGGCAGGCGCTATGCCAGACGGGGGTCTC TGAGCAGCTGGCCAGCCAGCAGCTCGAAAGGCTCCGGGTTGAGCATGAGCAAGAGAGGCGAGAAATGACG GGCAAGCTTGCCGCCCTGGAGAGCGCCCACAGAGCAAGCCTTGAGAGAGCAGATCAAGAGAAGGCGGAGA TGAGCACAGAAATCTGCAGGCTTCAGAATACAGTCAAGGACATGCAGCAGGCAGCATCTCTCTTGATGCT TCAGGGTGGCTGCCAGGCAACGGCAGGGGAGGAGGCAGAAGGAGATGGAGCCATGTCCCTGCTTCAGCAA GGCGAGCAGCTATTGGAAGAAAACGGAGATGTCCTCATCAGCCTGCAGAGAGCTCACGAGCATGCAGTGA AGGAAAATGCAAAGATGGCTACTGAAATTTCCCGATTGCAGCAGAGGCTGAAGAAGTTAGAGCCGGGGTC GGTGATCTCGTCTTGTTTGGAGGAGGGGACGTCTGAGATTTCTGGAAGTTCCAGGGAACAAGTAGAACCC ATAATGAAACAAGGTCCAGCCACCAAGCACTTCCTGAGCGATCTAGGAGACCACGAGGCCCGGGACCTGG CAAGTACCGGGACAAGCTCCGTTCAGAGGCAGGAGTGCAAAACCGAGGCGTCGGAAGCTTCCCTGGACTG CTTTTCTGAGCTTGAAAACAGTGAAGACACCAGGACCGAGTCCTGGGACCTCAAGAGTCAGATCAGCCAG CTTCGGGAGCAACTGACGGTCTTGCGAGCAGACTGCGATCGGGCTTCCGAGAGGAAACAGGACCTGCTTT TCGATATCTCTGTGCTGAAAAAGAAACTGAAGATGCTGGAGAGACTCCCGGAGGCATCTTCCAGGTATAA GGTATTATACGAGGATGCCGCCCGAGAAAACTCCTGCCTTCAGGAGGAGCTGAGACTTGTAGAGACGCGC TACGAGGAGTCGCTGGACAGCAACAAAGAGCTTACGGCGGAGGTCTACAGGTTGCAGGATGAGATGAAGA AAATGGAGGAGGTGATGGAGACGTTCCTCAGCCTGGAGAAGAGTTACGATGAGGTCAAAGTGGAAAATGA GGAACTCCGTGCTCTGGTGTTGAGACTTCAAGGGAAGATGGAGAAGGTGCTGGGAAGGGCCGCCCTGCAG GGTGACAGCTACGCCCTCTGGGAAGCCCCTTCAGAAAACCTGGAGGTCGCCTCGGATGAAAAGATGCTTG AACTCCGTCAGACTCCGAAAGAGTGTACGCCCAAGGTCGTGAGCATGCACCACATCATAGAAGAGTGTAC ACAAGAAACCCAGTGCTGTGAGCAGGGAAGCACGAAGCTCCTAGCTAGAATAAAAGCACACGAAATCGCC TGGTTCCACAGAGCAATTAAGACACATCCGGAAAAGCCTAGCGCGCAGAACCGAGTCATCCCGGAGGGAA GTGCTGCTCTCCTAGGCCTACAAGACAAGCATCTTCAGCAGGAGGCCACCATCGCAGAGTTAGAACTAGA GAAGCAAAAGCTACAGGAGCTGACTAGAAATCTGAGGGAGCGAGTCACTGCACTAGTTAGGCAAAAAGAC GCCCCTTCTCAAGGGCAGAAGGAGGAGGAGCTGAAGGCAATGATGCAGGACCTGCAGATCACGTGCGGGG AGATGCAGAGGAAGGTTGAACTCCTGAGATATGAATCTGAAAAGCTTCAAGAGGAAAATTCTATTTTGAG AAATGAAATTACTACTTTAAATGAAGAAGATAGCATCTCTAACCTGAAATTGGAGGAATTAAATGGCTCT CAGGAAGAATTGTGGCAGAAAATAGAAACCATAGAGCAGGAAAAAGCTTCAATTCAGACAATGGTTGAAA AATTAAAGAAACAGGTTTCAGATTTAAAAATCAAAAACCAACAGTTGGATTCAGAAAATATAGAACTCAG CCAAAAGAACTCCCAAAACAAGGAAGAACTGAAAACCCTTAATCAACGTCTGGCAGAAATGCTGTGCCAG AGGGAGGAGCCAGGAGCTTGCACCTCTGAGAAATGGGAACAAGAAAATGCAAGCCTCAAAGAAGAACTGG ATCACTACAAAGTGCAGACTTCCACTTTGGTGTCTTCTCTGGAGGCAGAACTTTCCGAAGTTAAACTGCA GACTCACGTCATGGAGCAGGAAAACCTCCTTCTCAAAGATGAACTGGAGAGACTGAAGCAACTGCACAGA TGTCCTGACCTCTCTGACTTCCAGCAAAAAATGTCTAGCATTCTAAGCTACAATGAAAAACTGCTGAAGG AAAAAGAAGTTCTGAGTGAAGAGTTAAAGAGCTGTGCAGATAAGCTGGCAGAGTCAAGCCTCTTAGAGCA CAGAATTGCTACAATGAAGCAGGAGCAGACCGCCTGGGAAGAGCAGAGTGAGAGCCTGAAGTCACAGCTG GCAGTGTCGCAGGCAAAGGTTCAGAACTTAGAAGATGTCCTGCAGAATGTCAACCTTCAGATGGCTGAGA TTGAATCAGATCTCCAAGTGACTCGACAGGAAAAGGAGGCGCTAAAGCAGGAAGTGATGTCATTACACAG ACAGCTTCAGAATGCCATTGATAAGGACTGGGTCTCGGAAACAGCTCCCCATCTCTCAGGGCTCCGGGGC CAGCAGAGAAGGCTGTCCTGGGACAAACTGGACCATCTGATGAATGAGGAACCACAGCTGCTTTGTCAAG AAAGCAAGAGACTCCAGACTGTGGTACAGAATACCCAAGCAGATCTCACCCACTCCCGGGAGAAGGTCCG TCAACTGGAATCCAACCTCCTTCCCACCAAACACCAAAAACAACTCAACCAACCATGTACTGTGAAGTCC ACAGAACAAGAAAAACTGACCTTAAAGAGAGAGTGTGAACAGTCTCAGAAAGAACAATCTCCTACCAGCA GGAAGGTGGGTCAGATGGGTTCCCTTGAGCGAGGATTAGAAACAATCCATTTGGAAAACGAAGGTCTGAA GAAGAAACAGATGCAGCCCCTGAGGTCCACTGTGACGCGTAGCCCATCCTCTCACTGGGATCTGCAGTTG CTCCAGCAGCAAGCCTGTCCGATGGTGCCCAGGGAGCAGTTTCTGCAGCTTCAACAGCAGCTGCTGCAGG CAGAAAAGAGAAGCCAGCACCTGCAGGAGGAGCTCGAAAACAGAACCTCCGAAACCAACACACCACAGGG AAACCAGGAACACCTTGTAAATCTCATGGAAGAACGAATGATAGAAGTTGAACAAAAACTAAAGCTGGTG AAGAGACTTCTTCAAGAAAAAGTAAACCAGCTCAAAGAACAACTCTGCAAGAACACAAAAACAGATGCCG TGGTGAAGGATTTGTATGTCGAAAATGCCCAGTTGTTGAAAGCTCTGGAGATGACTGAACAGCGCCAGAA GACAGCAGAAAAGAGAAATTTCCTCCTAGAAGAGAAGATTGCCAGCCTCAGTACCATCGTCAGGAACCTG GCACCAGCACCACTGACTTCTATGCCTCCTCTGAGGTCATAG ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA TTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-NotI
ACCN	NM_001081453
Insert Size	6342 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001081453.1, NP_001074922.1
RefSeq Size	9675 bp
RefSeq ORF	6342 bp
Locus ID	18080
UniProt ID	Q61043
Gene Summary	Centrosomal protein required for the positioning and anchorage of the microtubule minus-end in epithelial cells (PubMed:15784680, PubMed:10934040). May also act as a centrosome maturation factor (By similarity). May play a role in microtubule nucleation, by recruiting the gamma-tubulin ring complex to the centrosome (PubMed:15784680). Overexpression does not perturb nucleation or elongation of microtubules but suppresses release of microtubules (By similarity). Required for centriole organization and microtubule anchoring at the mother centriole (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) lacks an in-frame internal segment and the 3' terminal exon but includes an alternate 3' terminal sequence, compared to variant 2. The resulting isoform (1) is the longest; it lacks an internal segment and has a longer and distinct C-terminus, compared to isoform 2.

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