Myo7b (NM_032394) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225173 representing NM_032394 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCGTGTTCCGGCTGGGTGACCATGTGTGGCTGGACCCTCCCTCCAGCAGCAAGACTGGTGTGGCCA TTGGGGGCATTGTCAAAGAAACAAAACTGGGAAAGACCTTGATTGAGGATGACGAGGGCAAGGAACACTG GGTCCATGCAGAAGACCTCAGCACCCTCAGGCCTATGCACCCCAACTCAGCCCAAGGAGTGGATGACATG ATCCGCCTAGGGGACCTGAATGAGGCTGGTGTGGTGCACAACCTCCTGATCCGGTACCAGCAGCACAAGA TCTATACCTACACAGGCTCCATCCTGGTGGCTGTGAACCCATTCCAGATGCTGCCCCTGTACACCCTGGA GCAGGTACAAATCTACTACAGCCGCCACATGGGCGAGCTGCCTCCCCACATCTTCGCCATTGCCAACAGC TGCTACTTCAACATGAAGAAGAACAAGAGGGACCAGTGCTGTATCATCAGTGGCGAGTCTGGGGCCGGGA AGACCGAGACCACCAAGCTTATCCTTCAGTTCCTCGCCACGGTCAGTGGCCAGCATTCGTGGATCGAGCA ACAGGTCCTGGAGGCCAACCCCATCCTGGAAGCCTTTGGCAATGCCAAGACCATCCGCAATGACAACTCC AGCCGCTTCGGGAAGTACATTGACATCCACTTCAATTCTAGTGGGGTGATTGAGGGCGCAAGCATTGAAC ACTTCCTCCTGGAGAAGTCCAGAGTCTGCCGGCAGGCTCCAGAGGAACGCAACTACCACATCTTCTACTG CATGCTGATGGGGATGAGCCCGGAAGAGAAGCAAATGCTGAGTCTGGGCATGCCCTCTGAGTACCATTAC CTGACCATGGGGAGCTGCACATCCTCCGAGGGGCTCAGCGATGCCAAGGACTACGCACACGTCCGCTCGG CCATGAAGATCCTCCAGTTCTCAGACTCAGAGAACTGGGACATCAGCAAGCTGCTGGCAGCCATCCTCCA CCTGGGGAATGTGGGGTTCATGGCTGCAGTCTTTGAGAACCTGGATTCGTCTGATGTGATGGAAACTCCA GCCTTCCCCTTGGCAATGAAGTTGCTAGAGGTGCAGCACCAGGCTCTCCGAGACTGTTTGATCAAACACA CAATCCCTGTCCTTGGAGAGTTTGTCTCCAGGCCTGTGAACATCGCCCAGGCTACAGACCGGAGGGATGC CTTTGTCAAGGGCATCTATGGGCGCCTCTTCCAGTGGATTGTCAAGAAAATCAATGCTGCGATCTTCACT CCCCAAGCCCAGGATCCCCAAAATGTGCGGAGGGCCATCGGTCTCCTGGACATATTTGGCTTTGAAAACT TCCAGAACAATAGCTTTGAGCAACTCTGCATCAACTTTGCCAACGAGCACCTTCAGCAATTCTTCGTGAA GCACGTGTTCACGATGGAGCAGGAAGAGTACCTGTCTGAGAACATCACCTGGAACTACATCCACTACACC GACAACCAGCCCATCCTGGACATGCTGGCCCTCAAGCCCATGAGCATCATCTCTCTCCTAGATGAGGAGA GCCGCTTCCCACAGGGGACAGATGTCACTATGCTGCAGAAACTGAACAGTATCCATGCCAACAACAAGTC CTTCCTGAGCCCCAGGAGCATCCATGACACCCGATTTGGGATCGCACACTTCGCGGGCGATGTATACTAC CAGGCAGAAGGCTTTCTGGAGAAGAATCGAGATGTGCTGAGCACAGATATCCTAATCCTGATTCATTCCT CGAAAAACAAGTTTCTGAAGGAGATATTTAACGTGGACTCATCACAGACTAAGCTGGGGCACGGCACCAT CTGCCAGGTGAAGGCAGGAAGCCAGCTCTTCAAGTCATCAGACTCCATCAAGCGCCCCGTCACCCTCGCG AGCCAGTTCAAGCAGTCTCTGGACCAGCTGATGAGAATCCTGACCAACTGCCAGCCCTATTTTGTCCGCT GCATTAAACCCAATGAGTACAAGAAGCCACTGCTGTTTGACCGAGAGCTGTGCATTCAGCAGCTGCGTTA TTCCGGCATGATGGAGACTGTGCACATCCGCAAGTCAGGTTTTCCCATCCGCTACACATTTGATGAGTTC TCACAGAGATTCCGTGTGCTGCTGCCCAGCCCGGAGCGTATGCAGTTTCAGAACAAGCCCCGCCAGATGA CCTTGCACATTGCTGACCTATGCCTGGGGACAGACAAAGACTGGAAAGTCGGAAAGACCAAAATCTTCCT GAAGGATCATCAGGACACAGTGCTGGAGATCCGCAGGAGCCAGGCCCTGGACGGAGCAGCGATCCGGATC CAGAGAGTCCTCCGGGGACACAAGTACAGGAAGGAGTTCCTGAGGCAGAGACGTGCAGCTGTGACCCTCC AGGCAGGGTGGCGAGGCTATAGCCAAAGAAAGAATTTCAAGCTGATCCTAGTGGGCTTTGAACGCCTGCA GGCCATCGCCCGGAGCCACCTGCTGATGAGACAGTTCCAGGCCATGCGACAGAGGATAGTGCAGCTGCAG GCCCGATGCAGGGGCTACCTGGTGCGACAGCAAGTCCAGGCCAAGAGGAGGGCAGTGGTGATCATTCAAG CACATGCCAGGGGCATGGTTGTCCGGAAGAGCTACTGGCAACAGAAAAGCACTGGGCCTCAGGTCATTCT GGCTAAGGAACCAAAGGCTCAAGTTGCGGTCCATGAGAGGAAGCGTAAGTCCATCTATGACACTGTCACT GACACAGCGATGGTGGAGAAAGTGTTTGGCTTCCTCCCAGCCATGATTGGAGGTCAGGAGGGTCCAGCCC CAACACGCTTTGAGGATCTGGAAGTAAAGACCCAGAAACTTCATGAGGTTGATCTGGATACAGTGCCCAT GATGGCGATGCCAGAAGAGGAGGTGGACAGCCTGGCTGAGTATACCTTCCCCAAGTTTGCTGTGACTTAC TTCCAGAAGTCAGCCAGCCACACCCACATCCAGAAGCCCCTTCGATACCCACTCCTCTACCATGAAAATG ACACTGACCACTCGGCTGCTCTAGATGTTTGGATTATCATCCTGAGGTTCATGGGTGACCTTCCAGAGCC AGTGGTCTATGGCAGGAACAGCCTGACTGGCAGCTCAGTGATGCGGCAGATCCATGACAAACTGGGCAAG GACAGTGTCACTCAGCACAACAGATCCTCACAGGTGGCCAGCCAGCTGAACTTTGGTGAAGAGGCATTCA AGTTTGATGGCCCTATCTCAGACAGACCCATGTCTAACCTAGAGAAGGTGCACTTCATTGTGGGCTACGC CATCATGCGTCCTGGACTCAGAGATGAAATCTACTGTCAGATCTGCAAGCAGCTCTCCGAGAACTACAAA ACAAGTAGCCGGGCCCGGGGCTGGATCCTGCTCAGCCTCTGTCTGGGATGCTTCCCACCCTCAGAGAGGT TCATGAAGTATCTGCTGAATTTCATTAGTCAAGGGCCACCCAGCTATGGGCCCTTCTGTGCTGAACGGCT GCAACGCACCTTCGCCAATGGGGTGCGCGCAGAACCCCCCACCTGGCTGGAGCTGCAGGCTGTCAAGTCC AAGAAGCACATCCCGATCCAGGTCATCCTGGCAACCGGAAGGAGCCTGACCATCTCTGTGGACTCCGCCT CCACATCACGAGAAATCTGCCAGCACGTCGCTCAGAAACAGGGTCTCAGGGACAATCTGGGCTTCTCCCT CCAGGTCGCTGTATATGATAAGTTCTGGTCCTTGGGCAGCGGCTGTGACCATTTGATGGATGCTGTGGCC CAGTGTGAGCAACTGGCCCGAGAGAGGGGAGAGAGCCAGCGGCAGGCCCCCTGGCGTATCTACTTCCGGA AGGAGTTCTTCACCCCCTGGCATGACTCCCAGGAGGACCCTGTGAGCACTGAGCTCATTTACCACCAAGT CCTCCGTGGGGTGTGGTCTGGCGAGTATAACTTTGAGAAGGAGGAGGAGCTTGTGGAGCTGCTGGCCCGG CACTGCTATGTGCAGCTTGGGGCCACGGTGAAGAGTAACGCTGTCCAGGAGCTGTTGCCCAGCTGTGTTC CCTCCAAACTGTACCGGACCAAGTCTCCAGAGAAGTGGGCTAGCCTTGTCACCGCTGCCCATGCCAAGGC CCAGTATACCCAGTCGAAAGCCACGCCACTGGCGGTGAGGGAGCAGACAGTGGAGGCTGCCCGTCTTCTG TGGCCCCTGCTCTTCTCCCGGCTATTTGAAGTCACCACACTCTCTGGTCCCCGCCTTCCCAAGACGCAGC TGGTTTTAGCCATTAACTGGAAGGGGATGTACTTCCTGGATCAGAAGGAGAGGACACTGCTGGGGTTGTC CTTCGCAGAGGTCATGGGTCTGGTCGCCAACAGGGACGCCCCAGGAGGGAAGAAGCTGCTTCTCGCCACA CTCCAGGAAGAGTACGAGTTCGTCTCCCCCAGCAGCGTGGCTATTGCAGAGATGGTGGCCTTGTTCTTGG GAGGCTTGAAGGAGAGATCTGTGTTCGCCATGGCCCTGCAGGACAGAAGGGCCACAGACGATATCACCCT TCTGCCCTTCAAGAAAGGTGACTTACTGATCCTGACCAAGAAACAAGGACTGTTGGCCTCAGAGAACTGG GCTCTGGGTCAGAATGACAGAACTGGCAAGACAGGGCTGGTGCCTACTGCCTGTCTCTACACCATCCCCT CGGTCACCAAGCCCTCAACCCAGCTGCTGAGCTTGCTGGCCATGTCACCAGAGAAACGGAAGCTGGCAGC CCAGGAGGTGCGGGCTCTGGAGCCACCACTGGAGGATCAGCTCACAGAGTCGCCATACACCCTGGAGGAA TTCTCCTACCAGTTCTTCAGGGCTCCGGAGAAAGAGACTATCAGCAGGGCTGCGATGCCCATGGCCCGAA GCCGGGGTCATCTCTGGGCCTACTCCCCAGAGCCGCTGCGCCAGCCACTGCTCAAATCTGTCCACGACAA AGCCAAACTGCGGGATGCAGCCTGCCAGATCTTCCTTGCCATCCTCAAGTACACAGGTGACTACCCTTCC CGCCAGTCCTGGCACAGCCTGGAGCTCACAGACCAGATGTTCTCGTTAGCCCTGCAGGACCCAGCCCTGC AGGATGAGCTGTACTGCCAGATCCTGAAGCAGCTGACCCACAACTCCATCAGGTTCAGTGAAGAGCGAGC TTGGCAGCTACTCTGGCTCTGCACAGGTCTCTTCCCACCTGGGAAGACATTGCTGCCCCACGCTCAGAAG TTCATAGACTCCCGGAAGAAGAAGCCGCTGGCTCTCGACTGTAGCCGCAGGCTCCACAGGGTTCTAAGGG TGGGACCTCGGAAGCAGCCCCCACATGATGTAGAGGTGAAAGCGGCTGAGCAGAATGTCTCCAAATTACA CCATGAGGTCTACCTCCCCAATGACACCAGCAAGTCCATGGAAGTGGGCAGCAGTAGTCGGGTGAGAGAC CTATGTGAGGGCATAGGCACCAGGCTCCAGCTGGCCTCCTGGGATGGCTGCAGCCTCTTCATCAAGATCA CAGACAAGGTCATCAGTCTGAAGGAAGGAGACTTCTTTTTTGACAGCTTGAGGCAGGTATCTGACTGGGT GAAGAAGAACAGGCCCCAGAAAGAAGGGGCCTCCGTGACACTTCCCTATCAGGTGTTCTTCATGCGGAAA CTGTGGCTCAACGTGACCCCTGGGAAGGACGTGAATGCAGACACCATACTCCATTACCACCAGGAACTAC CCAAGTACCTCCGCGGGTTTCACAAGTGCTCCCGGGAAGATGCCATCCACCTGGGAGGTCTCATCTGTAA AATCCAGTTTGGTAGTGACAGCTCGCAACTGGCTAGTGTCTCAAAGGTCCTGAAGGAGCTGGTACCTCAG AACCTCACACGCCTGATGTCCTCAGAAGAATGGAAAAAGAGCCTCCTTCTGGAATGTGACAAAAACAAGC GCAAGACAGTGGCAGAGGCCAAAGTGGAGTTCTTGAAGTACATGTACAGGTGGCCCACCTTTGGGTCTGC CTTCTTTGAGGTGAAGCAAACCTCTGAGCCTTCCTACCCAGACATTCTCCTCATCGCCATCAACCGACAT GGGTTACTGCTCATCCACCCCAAGACCAAGGAGCTGCTGAACACCTACCCTTTCACCAAGATCTCCAGCT GGAGTAGTGGCAACACCTATTTCCACATGGCACTGGGGAGCCTGGGCCAGGGCAGCCGTTTGCTGTGTGA GACCTCTCTGGGTTACAAGATGGACGACCTGCTGACCTCATATGTACAGCAGCTCCTGAATACAGTGAAC AAACAGCGGGGCTTCAGGGCTCCCGCCCCGGCCAACCCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_032394
Insert Size	6342 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_032394.3, NP_115770.2
RefSeq Size	6591 bp
RefSeq ORF	6342 bp
Locus ID	17922
UniProt ID	Q99MZ6
Gene Summary	Myosins are actin-based motor molecules with ATPase activity. Their highly divergent tails are presumed to bind to membranous compartments, which would be moved relative to actin filaments. As part of the intermicrovillar adhesion complex/IMAC plays a role in epithelial brush border differentiation, controlling microvilli organization and length. May link the complex to the actin core bundle of microvilli.[UniProtKB/Swiss-Prot Function]

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