Ckap5 (NM_029437) Mouse Untagged Clone

CAT#: MC225122

Ckap5 (untagged) - Mouse cytoskeleton associated protein 5 (Ckap5), transcript variant 2, (10ug)



  "NM_029437" in other vectors (2)

CNY 32,300.00


货期*
12周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 3110043H24Rik; 4930432B04Rik; D730027C18Rik; mKIAA0097; T25636
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC225122 representing NM_029437
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAGATGACAGTGAGTGGTTGAAATTACCAGTTGATCAGAAATGTGAACACAAGCTATGGAAAGCAA
GATTAAGTGGGTATGAAGAAGCCTTGAAGATCTTCCAGAAAATAAAGGATGAAAAGAGTCCAGAATGGTC
CAAATACTTAGGATTAATCAAAAAATTTGTCACTGATTCCAATGCAGTGGTTCAATTGAAAGGACTAGAA
GCTGCACTTGTCTATGTTGAAAATGCTCATGTTGCAGGAAAAACAACGGGAGAGGTTGTATCAGGTGTTG
TAAGTAAAGTCTTCAACCAACCTAAAGCCAAAGCCAAGGAACTGGGCATAGAGATTTGCCTCATGTATGT
TGAGATTGAAAAAGGAGAGTCTGTACAAGAAGAGCTTCTGAAGGGCCTGGATAACAAGAACCCCAAGATC
ATAGTGGCCTGTATAGAAACACTGAGGAAAGCCTTAAGTGAATTTGGTTCCAAAATCATCTCACTTAAGC
CAATTATTAAAGTGTTGCCAAAACTATTTGAATCTCGAGACAAGGCTGTTCGGGATGAAGCCAAATTATT
TGCTATAGAGATTTATCGATGGAATCGAGATGCTGTGAAACACACACTACAAAATATAAATTCTGTCCAG
TTGAAAGAATTAGAAGAAGAGTGGGTCAAGCTGCCAACAGGTGCTCCTAAGCCCAGTCGGTTTCTGCGTT
CCCAACAAGAACTAGAAGCTAAACTGGAACAGCAACAGTCTGCTGGTGGAGATGCAGAAGGGGGTGGTGA
TGATGGTGATGAGGTACCACAGGTAGATGCTTATGAGCTTCTGGATGCGGTAGAGATCCTTTCCAAACTT
CCCAAAGACTTTTATGACAAAATTGAGGCAAAGAAATGGCAAGAGAGAAAAGAAGCTCTGGAGGCTGTAG
AAGTACTAGTGAAAAACCCCAAGCTGGAAGCAGGCGATTACGCAGATTTAGTGAAAGCATTAAAAAAGGT
TGTTGGAAAGGACACCAATGTCATGTTGGTAGCTTTGGCAGCAAAATGTCTTACTGGCTTAGCTGTTGGG
CTCAGAAAGAAATTTGGACAATATGCAGGACATGTTGTGCCAACAATTTTGGAAAAATTCAAAGAAAAGA
AGCCTCAAGTGGTACAAGCCCTGCAGGAGGCAATTGATGCAATCTTCCTCACTACCACACTACAGAACAT
CAGTGAGGATGTCTTAGCAGTAATGGATAATAAAAATCCCACCATCAAGCAGCAGACATCACTTTTTATT
GCAAGAAGCTTTCGTCACTGCACTTCCTCTACATTGCCGAAGAGTCTACTAAAACCCTTTTGTGCTGCTC
TACTTAAGCACATCAATGATTCTGCTCCTGAAGTCAGAGATGCTGCATTTGAAGCACTGGGTACAGCTTT
GAAGGTGGTTGGTGAGAAATCAGTAAACCCATTTTTAGCTGATGTTGACAAACTCAAACTTGATAGGATC
AAAGAATGTTCAGAAAAAGTAGAACTGGTTCATGGTAAAAAATCTGGACTTGCAACTGAAAAGAAGGAAT
CCAAGCCTCTACCTGGAAGGGCTGCAGCATCAGGGGCTGCAGGGGACAAAGACACAAAAGATGTTTCAGG
ACCCAAACCAGGACCGTTAAAAAAAACACCTACTGCTAAGGCTGGTGGACCATCTAAGAAAGGTAAAACA
ACTGCACCAGGTGGCTCAGCAAGTGCCGGGACAAAGAATAAGAAAGGATTGGAGACCAAAGAAATAGTGG
AGCCAGAACTCTCAATTGAAGTATGTGAAGAGAAAGCTTCAGCTGTTCTTCCCCCAACCTGTATTCAGCT
TCTAGACAGCAGCAACTGGAAGGAAAGGCTGGCCTGTATGGAAGAATTCCAAAAGGCTGTTGAACTAATG
GAGCGAACTGAGATGCCGTGCCAGGCATTGGTGAAGATGCTAGCAAAAAAACCTGGATGGAAAGAAACTA
ATTTTCAGGTGATGCAGATGAAGCTTCACATTGTTGCTTTGATTGCCCAGAAGGGAAATTTTTCTAAAAC
GTCTGCTCAGATTGTATTAGATGGCCTTGTAGACAAGATTGGAGATGTGAAATGTGGGAACAATGCAAAA
GAAGCCATGACAGCAATAGCTGAAGCGTGCATGCTGCCCTGGACAGCTGAGCAGGTGATGTCAATGGCCT
TCTCACAAAAGAATCCTAAGAATCAATCAGAAACTCTAAATTGGCTATCAAATGCAATAAAAGAATTTGG
ATTTTCTGAGTTGAATGTCAAAGCTTTCATTAGCAACGTGAAGACTGCTCTTGCTGCAACAAATCCTGCT
GTGAGGACTTCTGCCATCACCCTGCTTGGCGTGATGTATCTATATGTTGGTCCTTCTTTGAGAATGATCT
TTGAGGATGAGAAGCCTGCCCTCCTATCCCAGATAGATGCAGAATTTCAAAAGATGCAGGGACAAAGTCC
TCCCGCTCCAACGAGGGGAATTGCTAAGCACAGCACAAGTGCTACAGATGAAGGAGAAGATGGAGAGGAA
CCAGGTGAAGGGGGCAATGATGTTGTTGATCTTTTGCCGAGGATTGAGATCAGTGACAAAATTACTTCAG
AGTTGGTGTCTAAGATTGGTGACAAGAATTGGAAGATCAGGAAAGAAGGCCTAGATGAAGTGGCAGGTAT
TATCAATGAAGCAAAATTTATCCAACCAAATATAGGTGAACTTCCAACTGCCTTGAAGGGTCGACTGAAT
GATTCAAACAAAATCTTGGTGCAGCAGACACTGAATATCCTTCAGCAACTGGCAGTAGCCATGGGTGCAA
ACATCAGGCAGCATGTAAAGAACCTGGGCATCCCTGTCATTACTGTTCTCGGAGATAGCAAGAACAATGT
TCGAGCTGCTGCCCTAGCAACTGTGAATGCTTGGGCAGAACAAACTGGCATGAAAGAATGGCTGGAGGGA
GAAGATCTTTCTGAAGAACTTAAAAAGGAAAATCCCTTCCTGAGGCAAGAGCTTCTGGGATGGCTGGCAG
AGAAACTACCTACACTCCGTTCCACTCCTACAGATCTTATCCTTTGTGTTCCTCATCTGTATTCCTGTCT
AGAAGATCGAAATGGAGATGTACGGAAGAAGGCCCAAGATGCTTTGCCATTCTTTATGATGCATTTAGGA
TATGAAAAAATGGCTAAGGCTACTGGGAAACTAAAGCCAACTTCTAAAGATCAAGTACTGGCCATGCTAG
AAAAGGCCAAAGCTAACATGCCATCCAAGCCTGCTGCACCTGCTAAGGCCATGTCCAAACCAATGGGAGG
GTCGGCTCCAGCCAAAACCCAGCCTATACCAGCACCTGTTGAAGATTCTGTTTCCAGTACCATAGAAGCT
AAGCCTGATCTGAAAAAGGCTAAAGCTCCAGGCGTTTCTTCCAAAGCAAAGAGTGTACAAGGGAAGAAAG
TGCCAAGCAAAACCACCTTAAAGGAAGACGACGACAAATCTGGTCCTATTTTTATTGTTGTTCCAAATGG
AAAGGAGCAAAGGATGAGAGATGAGAAAGGATTAAAGGTGCTAAAGTGGAATTTTACTACCCCACGGGAT
GAGTACATTGAACAGCTGAAGACTCAGATGTCTACCTGTGTGGCTAAATGGTTACAGGATGAAATGTTTC
ACTCAGATTTCCAGCATCACAATAAAGCCCTAGCTGTCATGGTTGATCACTTGGAAAGTGAAAAAGACGG
TGTCATCAGTTGCTTAGATCTTATCTTAAAATGGCTTACCCTCCGGTTTTTCGACACCAATACAAGCGTT
CTAATGAAAGCACTGGAATATTTAAAATTGCTTTTCACCCTGCTGAGTGAGGAAGAGTATCATCTTACTG
AGAATGAAGCATCGTCCTTTATCCCCTATCTCATCCTCAAGGTTGGAGAACCAAAGGATGTCATTCGTAA
AGATGTCCGTGCTATCCTGAACCGGATGTGCCTTGTCTACCCAGCCAGCAAAATGTTCCCCTTCATTATG
GAAGGAACCAAGTCCAAAAACTCTAAGCAGAGAGCAGAGTGCCTGGAAGAGCTAGGTTGCTTAATTGAGT
CCTATGGTATGAATGTTTGTCAGCCAACCCCAGGAAAAGCCTTAAAGGAGATAGCTATTCACATAGGAGA
CCGAGACAATGCTGTACGGAATGCTGCCCTCAACACCATTGTAACAGTGTACAACGTGCATGGAGATCAG
GTGTTCAAACTGATTGGAAATCTTTCTGAGAAAGACATGAGCATGCTTGAGGAGAGGATAAAGCGGTCAG
CAAAGAGACCCTCTGCTGCACCTGTGAAACAGGCCGAAGAGAAACCTCAGCGCACACAGAATATAAACTC
TAATGCCAACATGCTACGCAAGGGACCAGCTGAGGATATGTCCTCCAAACTTAATCAAGCTCGAAGCCTG
AGTGGGCATCCTGAAGCAGCCCAGATGGTTCGCCGAGAATTCCAGCTGGATTTAGATGAGATTGAAAATG
ATAATGGTACAGTCCGCTGTGAAATGCCAGAGCTTGTCCAGCACAAACTTGATGACATTTTTGAACCTGT
CCTTATTCCTGAACCTAAGATCCGGGCTGTTTCTCCACATTTTGATGACATGCACAGTAATACAGCATCC
ACAATCAATTTCATTATCTCCCAAGTAGCCAGTGGTGACATCAACACAAGCATCCAAGCTCTGACACAGA
TTGATGAGGTCCTGAGGCAGGAAGACAAAGCTGAAGCTATGTCTGGCCACATTGATCAGTTTCTGATAGC
AACATTCATGCAGCTAAGACTCATCTACAGCACACACATGGCAGATGAGAAGTTGGACAAGGATGAAATC
ATCAAGTTGTATAGCTGTATCATTGGCAACATGATTTCGCTATTTCAGATAGAGAGCTTGGCCCGGGAGG
CCTCCACTGGAGTACTGAAAGACCTGATGCATGGACTGATCACCTTAATGCTGGATTCTCGGATTGAAGA
TCTGGAGGAAGGACAACAGGTGATTCGTTCTGTGAACCTCTTGGTGGTGAAGGTTTTGGAGAAATCAGAC
CAGACCAATATCCTCAGTGCCCTGCTTGTTTTACTTCAGGACAGCCTGCTAGCAACAGCTAGTTCTCCCA
AATTCTCAGAGCTTGTTATGAAGTGTCTATGGAGAATGGTTCGACTATTACCTGACACCATCAACAGCAT
TAACCTAGACAGAATTCTTCTGGACATCCACATTTTCATGAAGGTCTTTCCCAAAGAGAAATTGAAGCAA
TGCAAAAGTGAATTTCCTATAAGGACTCTAAAGACCCTTCTACATACTTTATGCAAATTAAAAGGACCTA
AAATCCTAGACCACCTTACAATGATTGATAATAAGAATGAGTCAGAATTGGAGGCCCACCTGTGTCGGAT
GATGAAGCATAGTATGGACCAGACTGGGAGCAAGTCTGATAAGGAAACAGAAAAGGGAGCTTCTCGAATA
GATGAAAAATCATCAAAGGCCAAAGTAAATGATTTCTTAGCTGAGATTTTTAAGAAGATTGGCTCCAAAG
AGAACACTAAGGAGGGCCTAGCAGAGTTATATGAATATAAGAAGAAATATTCAGATACTGACATTGAACC
ATTTCTGAAAAATTCCTCGCAGTTCTTTCAGAGTTATGTTGAGAGAGGCCTTCGGGTGATTGAGATGGAG
AGAGAAAGCAAAGGCCGGATTCCTACTTCAACAGGTAACACAAATGGAGAAGAAGTGGGGCCATCTGTCT
ACTTGGAAAGGCTAAAAATTCTGCGGCAGCGGTGTGGTCTTGACAACACAAAGCAAGATGACCGGCCTCC
ACTGACCTCTTTGCTCTCCAAACCAGCAATTCCTCCAGTTGCCTCCTCCACAGACATGCTCCATAGCAAG
CTCTCACAGCTCCGGGAGTCCCGGGAACAGCACCAGCACTCAGACCTGGATTCCAACCAGACCCACTCTG
CAGGAACCATGACCTCCTCCTCCTCCACAACTAACATTGATGACTTGAAAAAGAGACTGGAGAGAATAAA
GAGCAGTCGCAAATGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_029437
Insert Size 6036 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_029437.2, NP_083713.2
RefSeq Size 6521 bp
RefSeq ORF 6036 bp
Locus ID 75786
UniProt ID A2AGT5
Gene Summary Binds to the plus end of microtubules and regulates microtubule dynamics and microtubule organization. Acts as processive microtubule polymerase. Promotes cytoplasmic microtubule nucleation and elongation. Plays a major role in organizing spindle poles. In spindle formation protects kinetochore microtubules from depolymerization by KIF2C and has an essential role in centrosomal microtubule assembly independently of KIF2C activity. Contributes to centrosome integrity. Acts as component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge. The TACC3/ch-TOG/clathrin complex is required for the maintenance of kinetochore fiber tension. Enhances the strength of NDC80 complex-mediated kinetochore-tip microtubule attachments.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 5' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...