Tet1 (NM_027384) Mouse Untagged Clone

CAT#: MC225115

Tet1 (untagged) - Mouse tet oncogene 1 (Tet1), (10ug)



  "NM_027384" in other vectors (2)

CNY 32,210.00


货期*
12周

规格
    • 10 ug

Product images

经常一起买 (3)
TET1 Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 2510010B09Rik; AA517754; BB001228; Cxxc6; D10Ertd17e; LCX; mKIAA1676
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC225115 representing NM_027384
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTCGGTCCCGCCCCGCAAAGCCTTCCAAATCAGTCAAAACAAAGCTACAGAAAAAGAAAGACATCC
AGATGAAGACGAAGACATCAAAGCAGGCTGTCAGACATGGGGCATCAGCGAAGGCAGTGAACCCCGGAAA
ACCGAAGCAATTAATTAAAAGAAGAGATGGTAAGAAGGAAACGGAAGACAAAACCCCGACACCGGCGCCG
AGTTTTCTGACGCGAGCCGGGGCCGCCCGAATGAATCGGGATCGAAACCAAGTTCTTTTTCAGAACCCAG
ACTCCTTAACTTGCAACGGGTTTACAATGGCTCTTCGAAGAACCTCTCTTAGCTGGCGACTCTCCCAGCG
CCCAGTAGTCACACCCAAACCTAAGAAAGTACCACCTTCAAAGAAGCAGTGTACACATAATATCCAGGAC
GAGCCGGGGGTAAAGCACTCTGAAAATGATTCAGTCCCCAGCCAACACGCTACCGTGTCCCCAGGCACAG
AGAATGGTGAACAGAACAGGTGTTTAGTTGAAGGGGAGAGCCAAGAGATAACCCAGTCTTGCCCTGTATT
TGAGGAAAGAATAGAAGATACCCAAAGCTGTATTTCCGCATCTGGGAACCTGGAAGCAGAAATTTCTTGG
CCACTGGAAGGGACACATTGTGAAGAACTACTTTCTCATCAGACATCTGATAATGAGTGCACTTCCCCTC
AAGAGTGTGCTCCATTGCCCCAAAGATCAACTTCCGAAGTTACCTCTCAGAAAAACACTAGCAATCAGTT
AGCAGACTTGAGCTCACAAGTAGAGTCTATCAAGCTGTCTGATCCTTCTCCGAATCCTACGGGAAGCGAC
CATAATGGCTTTCCAGACTCCAGTTTTAGGATCGTTCCTGAATTGGACCTGAAAACCTGCATGCCTCTTG
ATGAGTCTGTGTATCCCACAGCTCTGATAAGATTCATCTTGGCAGGCTCACAGCCGGATGTCTTTGATAC
TAAGCCCCAAGAAAAAACCCTTATAACAACCCCAGAACAAGTGGGAAGCCACCCAAATCAAGTTCTGGAT
GCCACCTCTGTCCTAGGACAGGCCTTTAGTACTCTTCCACTTCAGTGGGGATTTTCTGGTGCTAACTTAG
TTCAAGTTGAGGCCCTGGGCAAGGGTTCAGACTCACCAGAGGATCTTGGTGCTATTACTATGCTAAACCA
ACAAGAAACTGTGGCTATGGATATGGATAGGAATGCTACCCCGGACCTGCCTATCTTCCTTCCTAAGCCT
CCAAACACAGTTGCTACCTACAGTAGCCCTCTTTTAGGGCCTGAGCCCCACAGCTCTACCTCCTGTGGGC
TTGAAGTCCAAGGTGCTACCCCGATTTTAACTTTGGACTCAGGACACACTCCCCAACTCCCACCAAACCC
TGAGTCAAGTTCAGTACCTCTGGTAATAGCTGCAAACGGTACGCGAGCTGAGAAGCAGTTTGGTACCAGC
CTTTTCCCAGCCGTCCCACAAGGGTTTACAGTCGCAGCCGAGAATGAAGTCCAGCATGCCCCATTAGATC
TTACCCAGGGTTCTCAGGCTGCTCCCAGCAAACTGGAAGGAGAGATTTCTCGGGTCAGCATCACTGGCTC
AGCTGATGTCAAAGCCACAGCGATGTCCATGCCGGTTACACAGGCCAGTACCTCTTCTCCCCCATGTAAT
TCTACGCCGCCAATGGTGGAGAGGAGGAAGCGCAAGGCCTGTGGGGTCTGTGAGCCGTGCCAGCAGAAGG
CCAACTGTGGGGAGTGCACCTACTGCAAGAATCGAAAGAACAGCCACCAGATCTGTAAGAAGAGGAAATG
CGAGGTGCTCAAAAAGAAGCCAGAGGCCACCTCACAGGCACAGGTTACAAAAGAAAACAAGAGGCCCCAG
AGGGAAAAGAAGCCCAAAGTTTTAAAGACAGACTTTAACAACAAACCAGTAAATGGCCCCAAATCAGAGT
CCATGGACTGCAGTAGACGTGGTCATGGGGAAGAAGAGCAAAGGTTGGATTTGATCACACATCCCCTTGA
AAATGTAAGGAAGAATGCAGGCGGCATGACGGGAATTGAGGTGGAGAAGTGGGCGCCAAACAAGAAATCA
CACTTAGCAGAGGGTCAAGTCAAAGGAAGCTGTGATGCCAATTTAACTGGGGTGGAAAACCCCCAGCCCT
CTGAAGATGACAAGCAGCAAACCAACCCTTCTCCGACATTTGCCCAGACCATAAGGAACGGCATGAAAAA
TGTACACTGTTTACCCACTGACACACACCTTCCGCTCAACAAATTGAATCATGAGGAATTTAGCAAGGCG
TTGGGAAATAATTCCTCTAAATTGCTGACTGATCCCTCAAACTGCAAGGATGCCATGAGTGTCACCACCT
CCGGGGGGGAGTGTGATCATCTCAAGGGGCCAAGGAACACCCTGCTCTTCCAGAAGCCTGGCTTGAACTG
CAGGTCTGGTGCAGAACCCACCATCTTTAATAATCACCCTAATACACACAGCGCTGGGAGTCGACCTCAC
CCTCCTGAGAAGGTACCCAACAAAGAACCCAAAGATGGCTCTCCGGTTCAGCCAAGTCTTCTATCCCTAA
TGAAGGACAGGAGATTAACGCTGGAACAAGTGGTAGCCATAGAAGCCCTGACTCAGCTCTCAGAAGCGCC
CTCAGAGAGCTCCTCCCCATCCAAGCCGGAGAAGGATGAAGAAGCGCATCAGAAAACGGCTAGTCTGCTT
AACAGTTGCAAGGCTATCCTTCATTCGGTGAGAAAAGACCTCCAAGACCCAAACGTTCAAGGAAAGGGCC
TTCACCATGACACTGTTGTCTTTAATGGCCAGAACAGAACATTCAAGTCACCTGATAGTTTCGCTACTAA
CCAAGCACTTATAAAATCACAAGGATATCCAAGTTCACCTACAGCTGAGAAGAAAGGCGCTGCTGGTGGG
AGAGCTCCCTTTGATGGTTTTGAGAATTCACATCCATTGCCCATAGAAAGTCACAATCTTGAAAACTGCA
GTCAGGTACTAAGCTGCGATCAGAATTTGAGTTCCCATGACCCATCATGCCAGGATGCACCCTACAGTCA
AATTGAAGAAGATGTCGCAGCACAATTGACCCAACTTGCATCCACGATTAATCACATCAACGCCGAGGTC
AGGAATGCTGAAAGTACACCAGAAAGCCTTGTTGCAAAGAACACAAAGCAAAAACACAGTCAGGAGAAAC
GCATGGTACATCAGAAGCCACCCTCTAGTACACAAACTAAACCCAGTGTACCATCAGCAAAGCCAAAGAA
GGCCCAGAAAAAGGCAAGAGCGACCCCACACGCAAACAAGCGGAAAAAGAAACCCCCAGCCCGAAGCTCT
CAAGAAAATGATCAGAAGAAGCAAGAGCAGCTAGCTATAGAGTATAGTAAAATGCATGACATTTGGATGT
CATCTAAGTTTCAAAGGTTTGGCCAGTCTAGTCCACGTAGTTTTCCTGTTCTGTTGAGAAATATTCCTGT
CTTTAACCAAATATTAAAGCCCGTGACTCAGAGTAAAACACCCTCACAACATAATGAGTTATTTCCTCCT
ATCAATCAGATAAAATTCACAAGAAACCCTGAATTGGCAAAGGAGAAAGTGAAGGTTGAACCATCAGATT
CTCTGCCAACATGCCAATTCAAGACAGAATCCGGTGGGCAGACATTTGCAGAGCCGGCAGATAATTCTCA
GGGACAGCCCATGGTGAGTGTCAATCAGGAAGCTCACCCTCTGCCCCAGTCCCCACCATCTAACCAGTGT
GCTAATATAATGGCTGGTGCTGCCCAAACACAGTTTCATCTAGGCGCTCAAGAGAACCTGGTGCATCAGA
TACCACCACCGACGTTGCCTGGTACCTCCCCTGACACACTCTTGCCAGACCCAGCATCAATCCTCAGGAA
AGGGAAAGTGTTACATTTCGATGGAATTACAGTTGTTACGGAGAAGCGTGAAGCTCAAACATCAAGCAAT
GGACCACTGGGTCCCACCACAGACAGTGCACAGTCAGAATTTAAAGAGAGCATCATGGACTTACTTAGCA
AGCCTGCAAAAAACCTGATAGCTGGATTGAAGGAACAGGAAGCTGCACCCTGTGACTGTGATGGAGGTAC
ACAAAAAGAAAAAGGCCCATATTATACACACCTTGGGGCAGGACCAAGTGTGGCTGCTGTCAGGGAGCTC
ATGGAGACTAGGTTTGGCCAGAAGGGGAAGGCAATCCGGATTGAGAAGATAGTGTTCACGGGGAAGGAAG
GGAAGAGCTCTCAGGGCTGCCCGGTCGCCAAGTGGGTGATCAGAAGAAGTGGTCCTGAAGAGAAGCTTAT
TTGTTTGGTTCGTGAGCGTGTAGACCATCACTGTTCGACGGCTGTGATAGTTGTCCTTATCCTGCTGTGG
GAAGGTATCCCTCGCCTGATGGCTGACCGCCTGTACAAAGAGCTCACTGAGAACTTGAGGTCCTACAGCG
GACATCCCACAGACCGAAGATGTACCCTCAACAAAAAGCGTACCTGCACCTGTCAAGGCATCGACCCAAA
AACCTGCGGAGCGTCCTTCTCCTTTGGCTGTTCGTGGAGCATGTATTTCAACGGCTGTAAGTTTGGGAGG
AGTGAAAACCCCAGAAAATTCAGACTTGCTCCAAACTACCCCTTACATGAAAAACAGCTTGAAAAGAATT
TACAAGAATTGGCTACAGTATTAGCTCCACTTTACAAGCAGATGGCTCCAGTTGCTTATCAAAATCAGGT
GGAATATGAAGAAGTTGCTGGAGACTGTCGACTTGGAAATGAAGAGGGGCGTCCTTTCTCTGGTGTCACC
TGTTGCATGGATTTTTGTGCCCATTCTCACAAGGACATTCACAACATGCACAACGGAAGCACCGTGGTGT
GTACGTTGATTCGAGCAGATGGCCGTGACACAAATTGTCCCGAGGATGAACAACTCCACGTCCTGCCACT
ATACCGGCTTGCAGACACTGATGAATTTGGCTCCGTGGAAGGGATGAAGGCCAAAATCAAATCTGGGGCC
ATCCAAGTCAATGGGCCAACCAGGAAGAGGCGACTACGTTTTACTGAGCCTGTTCCTCGATGTGGGAAGA
GGGCCAAAATGAAGCAGAACCACAATAAATCAGGTTCACACAACACTAAGAGCTTTTCATCAGCCTCATC
TACTTCTCACCTAGTGAAAGACGAATCTACAGACTTCTGTCCCCTGCAGGCTTCCTCCGCAGAAACATCT
ACCTGTACGTACAGTAAAACAGCCTCAGGTGGGTTTGCAGAAACAAGTAGTATTCTCCACTGCACAATGC
CTTCTGGAGCACACAGTGGTGCTAATGCAGCTGCTGGGGAATGTACTGGAACGGTGCAGCCTGCCGAGGT
GGCTGCTCATCCTCACCAGTCTCTTCCCACAGCCGATTCTCCCGTTCATGCTGAGCCTCTCACTAGTCCA
TCTGAGCAGCTAACTTCTAACCAGTCAAACCAGCAGCTCCCTCTCCTCAGCAATTCTCAGAAACTGGCTT
CCTGTCAGGTGGAAGATGAGCGGCACCCTGAAGCGGATGAGCCTCAGCACCCCGAGGACGATAACTTGCC
TCAACTTGATGAATTCTGGTCAGACAGTGAGGAGATCTACGCCGATCCTTCCTTTGGTGGCGTGGCGATA
GCACCCATTCACGGCTCGGTGCTCATTGAGTGCGCTCGGAAGGAGCTTCATGCTACCACCTCTTTGCGCT
CCCCCAAACGAGGGGTCCCTTTTCGTGTGTCCCTTGTATTCTACCAGCACAAAAGCCTAAACAAGCCTAA
TCATGGTTTTGATATCAACAAAATTAAGTGTAAATGCAAAAAAGTAACGAAAAAAAAGCCCGCAGACCGG
GAGTGTCCTGATGTATCCCCCGAAGCCAATTTATCACACCAAATTCCTTCTCGAGTTGCATCAACCTTAA
CCCGAGACAATGTTGTTACCGTGTCCCCATACTCTCTCACTCATGTTGCGGGACCCTACAATCGTTGGGT
CTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_027384
Insert Size 6024 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_027384.1, NP_081660.1
RefSeq Size 13986 bp
RefSeq ORF 6024 bp
Locus ID 52463
UniProt ID Q3URK3
Gene Summary Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. In addition to its role in DNA demethylation, plays a more general role in chromatin regulation. Preferentially binds to CpG-rich sequences at promoters of both transcriptionally active and Polycomb-repressed genes. Involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT. Also involved in transcription repression of a subset of genes through recruitment of transcriptional repressors to promoters. Involved in the balance between pluripotency and lineage commitment of cells it plays a role in embryonic stem cells maintenance and inner cell mass cell specification. Plays an essential role in the tumorigenicity of glioblastoma cells. TET1-mediated production of 5hmC acts as a recruitment signal for the CHTOP-methylosome complex to selective sites on the chromosome, where it methylates H4R3 and activates the transcription of genes involved in glioblastomagenesis (PubMed:25284789).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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