Myh10 (NM_175260) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	5730504C04Rik; 9330167F11Rik; Fltn; mKIAA3005; Myhn-2; Myhn2; NMHC-B; NMHC II-B; NMHCII-B
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225095 representing NM_175260 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAGAGAACTGGACTGGAGGATCCCGAGAGGTATCTCTTTGTGGACAGGGCTGTCATCTACAACC CTGCCACTCAAGCTGACTGGACAGCTAAAAAGCTGGTGTGGATTCCATCGGAACGCCATGGTTTTGAGGC AGCTAGTATTAAAGAAGAGCGGGGCGATGAGGTTATGGTGGAGCTGGCAGAGAATGGGAAGAAAGCAATG GTCAACAAAGATGACATTCAGAAGATGAACCCACCAAAGTTCTCCAAGGTGGAGGATATGGCAGAGCTGA CATGCTTGAACGAAGCCTCTGTCTTACATAATTTGAAGGACCGCTACTATTCAGGACTTATCTATACTTA CTCTGGACTCTTCTGTGTGGTGATAAATCCTTACAAGAACCTTCCAATTTACTCTGAGAATATTATTGAA ATGTATAGAGGGAAGAAACGCCATGAGATGCCACCACACATCTACGCCATATCAGAGTCTGCTTACAGAT GCATGCTTCAAGATCGTGAGGACCAGTCAATTCTATGCACGGGTGAATCGGGTGCCGGGAAGACAGAAAA TACCAAGAAAGTCATTCAGTACCTTGCCCACGTTGCTTCTTCTCACAAAGGAAGAAAGGACCATAATATT CCTGGGGAACTTGAACGGCAGCTTTTACAAGCAAATCCAATTCTGGAATCCTTTGGAAATGCGAAGACTG TGAAAAATGATAACTCATCTCGCTTTGGCAAGTTTATCCGGATCAACTTTGATGTAACTGGCTATATTGT TGGGGCCAACATTGAAACATACCTTCTGGAAAAGTCTCGTGCTGTTCGTCAAGCTAAAGATGAGCGTACA TTTCATATCTTTTATCAGTTGCTCTCTGGAGCAGGGGAACACCTGAAATCCGACTTACTCCTGGAAGGTT TCAACAACTACAGATTCCTCTCCAATGGCTATATTCCTATTCCTGGACAGCAAGACAAGGATAACTTCCA GGAGACCATGGAAGCCATGCACATCATGGGCTTCTCTCACGAAGAGATCCTCTCAATGCTTAAAGTCGTA TCTTCAGTGCTGCAGTTTGGAAACATCTCTTTCAAAAAGGAGAGAAACACTGACCAAGCCTCCATGCCGG AGAACACAGTCGCACAGAAGCTCTGCCACCTGCTCGGGATGAATGTGATGGAGTTCACTCGGGCTATCCT CACGCCCAGGATCAAGGTTGGCCGGGATTACGTACAGAAAGCCCAGACCAAAGAGCAGGCTGATTTTGCA GTGGAAGCATTGGCAAAAGCTACCTATGAGCGGTTGTTTCGCTGGCTCGTTCACCGCATCAATAAAGCGC TGGATAGGACCAAACGCCAGGGAGCTTCCTTCATTGGGATCCTGGATATTGCTGGTTTTGAAATTTTTGA GCTGAACTCCTTCGAGCAGCTGTGCATCAACTACACCAACGAGAAGCTGCAGCAGCTGTTCAACCACACC ATGTTCATCCTGGAGCAGGAGGAGTACCAGCGAGAGGGCATCGAGTGGAACTTTATCGACTTCGGCCTGG ACCTGCAGCCCTGCATCGACCTGATAGAGAGACCTGCCAATCCCCCTGGCGTGCTGGCCCTCCTGGATGA AGAATGCTGGTTCCCCAAAGCTACAGATAAAACATTTGTTGAAAAGCTGGTTCAGGAGCAAGGTTCCCAC TCCAAGTTTCAGAAGCCGCGCCAACTGAAAGACAAAGCCGACTTCTGCATCATCCACTACGCGGGGAAGG TGGACTATAAGGCAGATGAGTGGCTGATGAAGAACATGGACCCGCTGAATGACAACGTGGCCACCCTCCT GCACCAGTCCTCGGACAGATTTGTGGCTGAGCTTTGGAAGGACGTGGACCGAATTGTAGGTCTGGATCAA GTCACTGGGATGACTGAGACCGCGTTTGGCTCTGCATACAAAACCAAGAAGGGCATGTTCCGAACCGTCG GGCAGCTCTACAAGGAGTCTCTCACCAAGCTGATGGCAACTCTCCGCAACACCAACCCCAACTTCGTCCG CTGCATCATTCCAAATCACGAGAAGCGGGCTGGGAAACTGGACCCGCACCTCGTGCTCGATCAGCTTCGC TGTAACGGCGTCCTGGAAGGGATCCGGATCTGTCGCCAGGGGTTCCCCAACCGGATAGTTTTCCAGGAAT TCAGACAGAGATATGAGATCCTAACTCCCAATGCTATTCCTAAAGGCTTCATGGATGGCAAACAGGCGTG TGAGCGAATGATCCGAGCTTTAGAACTGGACCCAAACCTGTATAGAATTGGACAGAGCAAGATATTTTTC CGAGCTGGAGTTTTGGCGCACTTAGAAGAAGAAAGAGATTTAAAAATCACTGATATCATCATCTTTTTCC AAGCTGTATGCAGAGGCTACCTCGCCCGAAAGGCCTTTGCCAAGAAACAGCAACAACTAAGTGCCTTAAA GGTCTTGCAGCGGAACTGTGCGGCGTACCTGAAGCTGCGACACTGGCAGTGGTGGCGTGTCTTCACGAAG GTGAAGCCTCTCCTCCAAGTGACCCGCCAGGAGGAAGAACTCCAGGCAAAAGATGAGGAGCTGCTGAAGG TGAAAGAGAAGCAGACAAAAGTGGAAGGGGAGCTTGAGGAGATGGAGCGGAAGCACCAGCAGCTGCTGGA AGAGAAGAATATCCTGGCAGAACAACTGCAAGCCGAGACCGAGCTCTTCGCTGAAGCAGAAGAGATGAGA GCAAGGCTTGCTGCCAAAAAGCAGGAACTGGAGGAGATTCTCCATGACCTCGAGTCCAGGGTGGAGGAGG AGGAAGAGCGGAACCAGATCCTACAGAATGAGAAGAAGAAGATGCAGGCGCACATTCAGGACCTAGAAGA ACAACTGGATGAGGAGGAGGGGGCCCGGCAAAAGCTGCAGCTGGAGAAGGTGACAGCAGAGGCTAAAATC AAGAAGATGGAAGAGGAGGTTCTGCTTCTCGAAGACCAGAATTCCAAATTTATCAAAGAAAAGAAACTCA TGGAAGACCGAATTGCTGAGTGTTCCTCTCAGCTGGCTGAAGAGGAAGAAAAGGCAAAAAACTTGGCCAA AATCAGGAATAAGCAAGAAGTGATGATCTCGGACTTAGAAGAACGCTTGAAGAAGGAGGAGAAAACTCGA CAGGAACTGGAAAAGGCCAAACGGAAGCTGGATGGGGAAACAACCGATCTGCAGGACCAGATCGCTGAGC TGCAGGCACAGGTCGATGAGCTCAAAGTCCAGTTGACCAAGAAGGAGGAGGAGCTTCAGGGGGCGCTGGC CAGAGGAGATGATGAGACACTGCACAAGAATAATGCACTTAAAGTTGCACGGGAGCTGCAGGCCCAAATC GCAGAGCTCCAGGAAGACTTTGAGTCTGAAAAGGCTTCAAGGAACAAGGCTGAGAAACAAAAACGGGACT TGAGTGAGGAGCTGGAAGCTCTGAAGACAGAGCTGGAGGACACCCTAGACACCACAGCAGCTCAGCAGGA ACTCCGCACAAAACGTGAGCAGGAAGTGGCAGAGCTGAAGAAGGCTCTTGAGGATGAAACTAAGAACCAC GAAGCTCAGATCCAGGACATGAGACAGAGGCATGCCACAGCGCTGGAGGAGCTTTCCGAGCAGCTGGAGC AAGCGAAAAGGTTCAAAGCCAACCTGGAGAAGAACAAACAGGGCCTGGAGACAGACAACAAGGAGCTGGC GTGTGAGGTGAAGGTGCTGCAGCAGGTGAAGGCGGAGTCAGAGCACAAGAGGAAGAAGCTGGATGCCCAG GTCCAGGAGCTCCATGCCAAGGTGTCAGAGGGTGACAGGCTCAGGGTAGAGCTGGCCGAGAAAGCAAACA AGCTACAGAATGAGCTGGATAATGTGTCAACCCTGCTGGAAGAAGCTGAGAAGAAAGGTATTAAGTTTGC GAAGGATGCAGCTGGTCTCGAGTCTCAACTACAGGACACACAGGAGCTCCTTCAGGAAGAGACACGGCAG AAACTGAACCTGAGCAGTCGGATCCGGCAGCTGGAGGAGGAGAAGAACAGCCTTCAGGAGCAGCAGGAGG AGGAGGAGGAGGCCAGGAAGAACCTGGAGAAGCAGGTGTTGGCTCTGCAGTCCCAGCTGGCTGACACCAA GAAGAAAGTGGACGATGACCTGGGGACAATCGAGAGTTTGGAGGAAGCCAAAAAGAAACTGCTCAAGGAT GTGGAGGCGCTGAGCCAGCGGCTGGAGGAGAAGGTCCTGGCGTATGACAAGCTGGAGAAGACCAAGAACC GGCTGCAACAAGAACTGGATGACCTGACGGTGGACCTGGACCACCAGCGCCAGATCGTCTCCAACTTGGA GAAGAAACAGAAGAAGTTCGACCAGCTGTTGGCAGAAGAAAAGGGCATCTCTGCTCGCTATGCAGAAGAG CGGGACCGGGCTGAAGCTGAGGCCAGAGAGAAAGAAACCAAAGCGCTCTCCCTGGCGCGGGCCCTTGAGG AGGCCTTGGAGGCGAAGGAGGAATTCGAGAGGCAGAACAAGCAGCTTCGAGCAGACATGGAAGACCTGAT GAGCTCTAAAGACGATGTGGGGAAGAACGTCCACGAGCTTGAGAAATCCAAGCGAGCCTTGGAGCAGCAG GTGGAGGAGATGCGGACCCAGCTGGAGGAGCTGGAGGACGAGCTGCAGGCCACTGAGGATGCCAAGCTCC GCCTGGAAGTCAACATGCAGGCCATGAAGGCCCAGTTTGAGAGGGACCTGCAAACCCGAGATGAGCAGAA TGAAGAAAAGAAGCGGCTGCTGCTTAAGCAGGTGCGGGAGCTCGAGGCAGAGCTGGAGGATGAGCGGAAA CAGCGGGCACTGGCTGTGGCGTCAAAGAAGAAGATGGAGATAGACCTGAAGGACCTGGAGGCTCAGATCG AGGCTGCGAACAAAGCCCGGGATGAAGTGATCAAGCAGCTTCGCAAACTTCAGGCACAGATGAAGGATTA CCAGCGTGAACTAGAAGAGGCTCGAGCATCTAGAGATGAGATTTTTGCTCAATCCAAAGAAAGTGAAAAG AAACTGAAGAGTCTAGAAGCAGAAATTCTTCAGCTGCAAGAGGAGCTGGCCTCATCCGAGCGAGCCCGCC GACACGCAGAGCAGGAGCGAGACGAGCTGGCTGATGAGATCGCCAACAGCGCCTCTGGAAAGTCTGCGCT GTTGGATGAGAAGCGGCGCCTGGAAGCGCGGATCGCACAGCTGGAAGAGGAGCTGGAGGAGGAGCAGAGC AACATGGAGCTGCTCAATGACCGCTTCCGCAAGACCACGCTGCAGGTGGACACACTGAACACAGAGCTGG CAGCAGAGCGCAGCGCTGCCCAGAAGAGTGACAATGCCCGCCAGCAGCTGGAGCGACAAAACAAGGAGCT GAAGGCCAAGCTGCAGGAGCTGGAGGGGGCAGTCAAGTCCAAGTTCAAGGCTACCATCTCAGCCCTGGAA GCCAAGATTGGGCAGCTGGAGGAGCAGCTTGAGCAGGAAGCCAAGGAGCGAGCAGCTGCCAACAAACTAG TCCGTCGAACAGAGAAGAAACTGAAAGAAATCTTCATGCAGGTTGAAGACGAGCGTCGGCATGCGGATCA GTATAAGGAGCAGATGGAGAAGGCTAATGCCAGGATGAAGCAGCTTAAACGACAGTTGGAAGAGGCTGAG GAAGAGGCCACACGTGCCAACGCATCTCGGCGTAAACTCCAAAGGGAGCTGGACGACGCCACTGAGGCCA ATGAAGGCCTGAGCCGCGAGGTCAGCACTCTCAAGAACCGGCTCAGGCGGGGCGGTCCAATCAGCTTTTC TTCAAGCCGATCTGGCCGGCGCCAGCTGCACATTGAGGGGGCATCGCTAGAGCTGTCAGATGACGACACA GAAAGTAAGACCAGTGATGTCAATGACACACAGCCACCCCAATCAGAATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_175260
Insert Size	5931 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_175260.2, NP_780469.1
RefSeq Size	7783 bp
RefSeq ORF	5931 bp
Locus ID	77579
UniProt ID	Q61879
Gene Summary	Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. During cell spreading, plays an important role in cytoskeleton reorganization, focal contacts formation (in the central part but not the margins of spreading cells), and lamellipodial extension; this function is mechanically antagonized by MYH9 (By similarity). Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping.[UniProtKB/Swiss-Prot Function]

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