Casp8ap2 (NM_011997) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	D4Ertd659e; FLASH
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225082 representing NM_011997 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCAGATGATGACAATGGTGATGGAACGGGTTTGTTTGATGTCTGTCCTGCCTCTCCACTTAAAA ATAATGATGAAGGCTCATTGGACATATATGCTGGGTTGGACAGTGCTGTTTCTGACAGTACTGCTAGATC CTGTGTGTCATTCAGAAACTGTTTAGATTTGTATGAAGAGATCCTGACTGAAGAAGGAACTGCGAAGGAG GCAACGTACAATGACTTGCAGATAGAATATGGGAAATGTCAGCAGCAAATGAAAGACCTGATGAAAAGGT TTAAGGAAATACAGACACAGAACTTGAACTTAAAAAATGAAAACCAGTCTCTTAAGAAGAATATCTCAGC ACTTATCAAAACTGCCAGAGTGGAGATAAACCGTAAGGATGAAGAGATAAATCATCTTCACCAAAGATTG TCTGAGTTTCCACATTTTCGAAATAACCATAAAACTGCAAGAACAAAAGATTCACAGTCCACATCTCCCC ATTTGGATGATTGTTCAAAGACTGATCACGGAGTTAAAAGTGATGTTCAGAAAGATGTACATCCTAACAC TGCACAGCCAAACTTGGAAAAGGAAGGAAAATCGCATTCTGAAGCACAGAATCCTTTGCACTTGTCTACG GGTGTTGAGAAACATTGTGCCAATAATGTCTGGTCACGTTCTCCTTACCAGGTTGGAGAAGGTAACTCAA ATGAGGATAATAGGAGAGGAAGGAGTGGCACTAGACATAGCCAGTGTAGCAGAGGAACTGATAGAACACA GAAAGACTTACATAGCAGCTGTAATGACAGTGAGCCAAGGGACAAGGAGGCTAACTCCAGACTACAAGGA CACCCTGAGAAACATGGCAACAGTGAAGCAAGGACGGAGAGCAAAATTTCAGAGAGTAAAAGCAGCACTG GTATGGGATATAAAAGTGAGCGCAGTGCCTCTTCTTGGGAAAAAGAGACTTCCAGAGAAAGGCCACACAC TCGAGTGGAATCTCAACATGACAAAAATCTAGAGAAACAAAATGAAAGATTACAAAATATGCACAGAAAA GAGCTTCCGTCTCAGGACAAAACAGAAAGAAAAGTTGATGTGAAGTTTAAACCAGCAGGAGAGGAGCAGG GGCATCGGGGAAGAGTGGACCGGGCGTTACCTCCTCATCCCAAGAATGACGTGAAACATTACGGCTTCAA TAAGTATCATCCAGAAGAGAGAAGGGGAAGGGAAGATTGTAAAAGAGACAGAGGGATGAACAGTCATGGC TTTCAAGATAGAAGATGTTCATCTTTTCTTTCAAGCAACAGAAATAGCAAATACCCGCACTCCAAGGAAG TTAGTGTTGCACACCAGTGGGAAAATACTCCTTTCAAAGCAGAAAGACATAGAACTGAGGACAGGAGGAA AAGAGAACGAGAAAACAAAGAAGAAAGTAGACATGTGAAAAGTGACAAAAAATCACCTCCAGAACACTTA CAAAGGACTCATAAAGACACTAAGAAAAGCACTGCTGATGGAAAGAGACAGACTGAGCCCAAACATGGTA AAGGTGCAGTCTCTAACAGTGAGCTTTCTAAAGGGACAGACAGTAAAGAAGGTGCAACAAAGGTGGAGAG TGGGCCAAATGAAGCAAAAGGCAAGGACTTAAAGTTAAGCTTCATGGAAAAACTGAACTTAACTCTTTCT CCTGCTAAAAAGCAGCCTGCTTGTCAAGATAACCCACATCAAATAACTGGTGTTCCCGAGCCCAGTGGCA CGTGTGACTCACGGTCCTTGGAGACGACTGGAACGGTGGCATGCCTTCCTTCTGGCAGTGAACATAATAG AGAGGAAACCAAATCAGAGTTACCAGAGCCAAAGGAGGCTCTTTTGGCAACATCTCAACTCAGGATCAGC ATTCCAGAAAACAAAATGAAGGAAGAAAAGAGGTTGTTATTTAAATCTGTTGAGAATACTGTGCCTTGTG AACTGCTTGCTTGTGGCACGGAAATTTCCCTCCCAGCACCTGTAGAAATTGAACAAGCAAGATGCTTGCT TGGATCAGTGGAAGTGGAAGAGACCTGTGGTGGTGCAAGGACAGCCGCTTCTGTGGTGATGCATGTGTTA CCAGAACATGCTTCTGAAGATGCCAGCCAAGAATTGGACACCAAAAGACACGATGGTATAAATGCTTGTG CTATTTCTGAAGGTGTGAAAACAAAGGTGATTCTTTCACCAAAAGCAGCTGCAGCCAGTGAGAGCCATCT TGCACCTTTGGTTGAAGAACCTAGCATTTCACTAGTAAACTGTTCGGGAGACAATAATCCTAAACTTGAA CCTTCTCTTGAAGAGAGGCCTATAGTTGAGACTAAATCCTGTCCTTTGGAGTCTTGTTTACCTAAAGAGA CTTTTGTACCTTCACCACAGAAGACTGAGTTGATTGACCACAAAATAGAAACTGGAGAATCAAACTCAGT ATATCAAGATGATGATAACTCAGTTTTGAGTATTGACTTTAATAATCTGAGACCTATTCCAGACCCCATC AGCCCTCTGAATAGTCCAGTGAGACCGGTATGCAAAGTTGTTAGCATGGAGAGCTCATGTGCAATTCCAC TGTATGATAGCAGTCATAAAGATGAGTTTCCATCCAATTCAACTCTTTCTACCTTCAAGAGTCAGTCTGA TCTAAATAAGGAAAATGAAAAACCAGTTCCCAAATTTGACAAATGTTCAGAAGCAGATTCTTGTAAGCAT TTGTCTTTAGATGAATTAGAAGAAGGCGAAATTAGAAGTGATGATGAAGAATCTGTAGCACAAAAACGCT TGGAAAAGAGTGCAAGACCTAGAGTTTCTGCTGAAGTGCAGCCAGGGAAAAGCAGCCCAGGAAGCAGGAG GAGCACTGTGCATGTGCATAAGGACAACGGGAGGACTGCTGTAAAACTCCCTCGGGACAGACTCACATGG AGCAAAAGATCAAGTGAATCAAGACCCTCCAACACCGAAAGGAAAAGTAAAACAATGAGCATCTCCAGCT TGGAAAAAATACTTCCGCTTATCCTTGTACCCTCTTCCCTGTGGGAGGTTATGCATATGTTACGGTTGCT AGGTAAACATGTAAGAAAAAATTATATGAAATTCAAGATAAAATTTTCATTGACACAATTTCATAGAATT ATTGAATCTGCAATTTTGAGTTTCACATCACTAGTTAAATGCCTTGATTTGTCTAAGATCTGTAAGTCAG TAAGTACTTTACAAAAGAGTCTTTGTGAAGTTATAGAATCTAACCTTAAACAAGTGAAGAAGAATGGCAT AGTTGACCGTTTATTTGAACAGCAACAAACAGATATGAAAAAAAAATTGTGGAAGTTTGTAGATGAACAA CTTGATTATTTGTTTGAAAAGCTTAAAAAAATCCTACTAAAGTTTTGTGATTCTGTAAACTTTGAAAATG AGAATAGTGAAGGAAAACTTGGAAAAAAATATAAAGAGAGAACCCAACATTCAAATTGTCAGAAGAAAAA AATGGACAACAAAGAAATACGGAGAGAAAAAGTGCTAAAATCAGAAAATACTGTGAATTTTAAATCTTCA CTGGGATGTGAAAAATCTGAAGAAAAACATCAGGACCAAAATAAAACCAATGCTAGTATAGTAAAGCATG ATGTAAAAAGAACTTTTAGCACTTGCAGTGATAATACAAAGAACGCTGAATGTAAAGAGCAGTTTCTGGA AAAGAGCTGCCCGAGTACCCCTAGGCCAGGAAAAGATGAAGGACATACTGAGGAGGAGGCACAGGCGGCA CAGCATGCAAGTGCTAAGTCCGAACGGAGCTTTGAGATCCTTACTGAGCAGCAGGCATCCAGCCTTACTT TTAACTTAGTGAGTGATGCACAGATGGGTGAGATATTTAAAAGCTTGCTACAAGGTTCTGATCTGTTGGA CACAAGTGGCACTGAAAAGGCAGAGTGGGAATTAAAGACTCCAGAGAAACAGCTGCTGGAAAGCCTCAAG TGTGAATCTGCACCAGCTTGTGCAACAGAAGAGCTGGTTTCAGAGGGGGCTTCTCTTTGTCCAAAAGTGA TCAGTGATGATAATTGGTCTTTATTATCATCTGAAAAGGGTCCATCTTTATCTTCAGGGCTTTCACTGCC AGTTCATCCTGATGTGTTAGATGAAAATTGTATGTTTGAAGTATCTTCTAACACTGCTTTAGGTAAAGAT AATGTATACAGCTCAGAAAAGAGTAAGCCCTGCATCTCTTCCATACTCTTAGAAGATCTTGCGGTCTCTT TAACAGTACCGTCACCTCTGAAATCAGATGGCCATTTGAGTTTCTTAAAGCCAGAAGTTTTGTCAACTTC AACTCCTGAAGAAGTTATTAGTGCACATTTTAGTGAGGATGCTTTGCTTGAGGAAGAGGATGCATCTGAA CAGGACATTCATCTAGCTCTGGAGTCTGATAACTCAAGCAGTAAGTCAAGCTGTTCATCATGGACAAGCC GGTCTGTTGCTTCAGGCTTTCAGTACCACCCTAATCTTCCCATGCATGCTGTCATAATGGAAAAGTCCAA TGATCATTTCATTGTGAAAATACGGCGTGCAACACCATCTACCTCCCCTGGCCTTAAACATGGTGTGGTA GCTGAGGAGTCATTGACATCTTTGCCTAGAACTGGAAAAGAAGCTGGTGTAGCAACAGAGAAAGAACCTA ACCTGTTTCAGAGTACAGTTTTAAAACCTGTCAAGGACTTGGAAAATACTGATAAAAATATTGATAAGAG TAAACTAACTCATGAAGAACAGAACTCTATAGTACAAACACAGGTTCCAGATATATATGAATTTCTTAAA GATGCCTCAAATAAGGTGGTTCATTGTGATCAAGTGGTTGATGATTGCTTCAAGTTGCATCAAGTATGGG AACCAAAAGTTTCTGAGAACCTTCAAGAGTTGCCTTCAATGGAGAAAATCCCACACTCTCTTGATAATCA TCTTCCTGATACACACATAGATCTAACGAAAGATTCAGCCACTGAGACCAAAAGCTTGGGGGAGCTAATG GAAGTAACAGTTTTAAATGTTGATCACTTGGAATGTTCTCAAACCAACTTAGATCAAGATGCAGAGATAA CATGTAGTTCTTTACAGCCTGATACTATAGATGCTTTTATTGATTTGACACATGATGCTTCAAGTGAGAG TAAAAATGAAGGTAGTGAACCTGTGTTAGCTGTTGAAGGTATGGGATGCCAGGTAATATGTATAGATGAG GATACCAACAAAGAAGGAAAGATGGGAAGGGCAAACAGTCCTTTAGAAAGTATTGTTGAAGAAACTTGTA TTGATTTGACCTCAGAGTCTCCTGGCTCCTGTGAAATCAAGAGACATAATTTAAAGTCGGAGCCTCCATC AAAGTTGGATTGTTTAGAGTTGCCTGAAACTCTGGGTAATGGTCACAAGAAGAGGAAAAACAGTCCTGGT GTTAGTCACTCTTCTCAGAAAAAACAAAGAAAGGACATAGACTTAAGTAGTGAAAAGACCCAGAGACTTA GTCCCAATTCTGATAGAAATGGTGATGCTCACAGAAAGCAAGCGAGCAAGAAAAGAGAACCTGCAGTAAA TGAAACGTCCTTGTCATCAGAGGCCAGCCCAGAGGTGAAGGGTTCAACAGCAGTACTTGCTGCTTCCCCA GCAAGCCTTTCTGCAAAAAATGTTATCAAAAAGAAGGGAGAAATTATAGTTTCATGGACAAGAAATGATG ACCGGGAAATTTTACTGGAATGTCAGAAAAGAATGCCGTCCCTGAAAACATTTACTTATTTAGCTGTCAA GCTGAATAAAAATCCAAATCAGGTTTCAGAGAGGTTCCAGCAGCTGAAAAAGCTCTTTGAGAAGTCAAAA TGCAGGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_011997
Insert Size	5889 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_011997.3, NP_036127.2
RefSeq Size	6813 bp
RefSeq ORF	5889 bp
Locus ID	26885
UniProt ID	Q9WUF3
Gene Summary	Participates in TNF-alpha-induced blockade of glucocorticoid receptor (GR) transactivation at the nuclear receptor coactivator level, upstream and independently of NF-kappa-B. Suppresses both NCOA2- and NCOA3-induced enhancement of GR transactivation (By similarity). Required for histone gene transcription and progression through S phase (By similarity). Required for histone gene transcription and S phase progression (By similarity). Involved in TNF-alpha-induced activation of NF-kappa-B via a TRAF2-dependent pathway. Acts as a downstream mediator for CASP8-induced activation of NF-kappa-B. Required for the activation of CASP8 in FAS-mediated apoptosis.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.