Myh7b (NM_001085378) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Myh14
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225069 representing NM_001085378 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGATATGAGTGAACTTGGAGAATCAGCCTGCTACCTCCGCCAGGGCTACCAGGAAATGATGAAGG TGCACACTGTCCCATGGGATGGTAAGAAACGGGTCTGGGTGCCTGATGAGCAGGATGCCTATGTGGAGGC TGAGGTCAAGACTGAAGCTACTGGAGGCAAAGTCACTGTGGAGACCAAAGACCAGAAGGTGCTGACAGTG CGTGAAACGGAGATGCAACCCATGAACCCACCCCGTTTCGACTTACTGGAGGACATGGCCATGATGACGC ACCTCAACGAGGCTGCAGTGCTGCATAACCTGCGGCAGCGCTATGCTCGCTGGATGATCTACACATACTC GGGCCTCTTCTGTGTCACCATTAACCCATACAAATGGCTCCCGGTCTACACAGCTGCTGTGGTGGCTGCC TACAAGGGCAAGCGTCGCTCCGAGGCCCCACCTCATATATATGCAGTGGCAGATAATGCCTACAACGACA TGCTCCGCAACCGAGAGAATCAGTCCATGCTGATCACCGGAGAGTCGGGGGCCGGTAAGACGGTTAACAC CAAGCGGGTCATTCAGTACTTTGCCATCGTCGCTGCCCTGGGAGACGGGCCGGGCAAGAAGGCACAATTT CTGGCGACAAAGACAGGGGGCACCCTTGAGGATCAAATCATCGAGGCTAACCCTGCCATGGAGGCTTTTG GCAACGCCAAGACTCTGAGGAACGACAACTCCTCCCGTTTTGGCAAGTTCATCCGCATCCACTTTGGTCC CACTGGGAAGCTGGCCTCTGCGGACATTGATAGCTATCTCCTGGAGAAGTCGCGAGTGATCTTCCAGCTG CCCGGTGAGCGTGGTTACCATGTCTACTACCAGATCCTCTCAGGGAAGAAGCCGGAGCTGCAGGACATGC TGCTCCTGTCCATGAATCCCTACGACTACCACTTCTGCAGCCAGGGGGTCACCACTGTGGACAACATGGA TGACGGCGAGGAACTTATCGCCACTGATCATGCAATGGATATCCTGGGCTTCAGTGTGGATGAGAAGTGT GCCTGCTATAAGATCGTGGGTGCTCTCCTGCACTTTGGCAACATGAAGTTCAAACAGAAGCAGAGAGAGG AACAGGCTGAGGCAGACGGCACTGAGAGTGCGGACAAGGCTGCCTACTTGATGGGTGTCAGCAGCGGGGA CCTCCTCAAAGGTCTCCTGCATCCCCGGGTTCGTGTGGGAAATGAGTATGTTACCAAGGGCCAGAGTGTG GAGCAGGTGGTATTTGCCGTGGGGGCCCTGGCCAAGGCTACCTACGACCGGTTGTTCCGATGGCTGGTTT CTCGAATCAATCAGACCCTGGACACAAAGCTCCCTCGACAGTTCTTTATCGGGGTCCTGGACATCGCTGG TTTTGAGATTTTTGAGTTTAACAGCTTCGAGCAGCTGTGCATCAACTTCACCAACGAGAAGCTGCAGCAG TTCTTCAACCAGCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGCGGGAGGGCATCGACTGGGTCTTCA TCGACTTCGGCCTTGACCTGCAGCCTTGTATCGACCTCATCGAGAAGCCTCTGGGCATCCTGTCCATCCT GGAGGAGGAGTGCATGTTCCCCAAGGCTTCAGACGCAAGCTTCAGGGCCAAGCTCTATGACAATCATTCT GGGAAGTCACCTAATTTCCAGCAGCCTCGCCCAGACAAGAAACGCAAGTACCAGGCTCACTTCGAGGTGG TCCACTACGCAGGCGTGGTTCCTTATAGCATCGTGGGCTGGCTGGAGAAAAACAAGGATCCCCTCAATGA GACGGTGGTCCCCATCTTCCAAAAGTCACAGAATAGGCTCTTGGCCACCCTCTATGAGAACTATGCAGGT TCTTGCTCCACCGAGCCCCCCAAGTCTGGGGTGAAAGAGAAGCGCAAGAAAGCAGCATCATTTCAGACCG TGTCTCAGCTTCACAAGGAGAACCTCAACAAGCTCATGACCAACCTGCGGGCCACACAGCCCCATTTCGT CCGCTGTATCGTCCCCAACGAGAACAAGACTCCAGGGGTCATGGATTCCTTCTTGGTGTTACACCAACTA CGCTGCAACGGGGTCTTGGAGGGAATCCGGATCTGCCGCCAAGGGTTCCCCAACAGACTGCTGTATGCTG ACTTCAGGCAGCGGTACCGAATCCTGAACCCCAGTGCCATCCCAGACGACACCTTTGTGGACAGCAGAAA GGCCACAGAGAAGCTGCTGGGCTCCCTGGACATCGACCACACTCAGTATCAGTTTGGCCACACCAAGGTG TTCTTCAAGGCTGGGCTTCTAGGCATCCTGGAGGAGCTGCGGGACCAGCGTCTGGCTAAGGTGTTGACAC TGCTGCAGGCTCGGAGCCGGGGCCGCCTCATGCGGCTGGAGTACCAGCGCATGCTAGGGGGAAGGGATGC CCTGTTCACCATACAGTGGAACATCCGGGCCTTTAATGCCGTCAAGAACTGGTCCTGGATGAAGCTCTTC TTCAAGATGAAGCCACTGCTGCGCTCAGCACAGGCGGAGGAGGAGCTGGCGGCACTGCGGGCAGAGCTGC GGGGGCTGCGAGGGGCCCTGGCTACGGCTGAGGCTAAGCGGCAGGAGCTGGAGGAGACTCAAGTCAGTGT CACTCAGGAGAAGAACGACCTGGCCCTGCAGCTGCAGGCAGAGCAGGACAACCTCGCAGATGCAGAGGAG CGCTGCCACTTGCTGATCAAGTCCAAGGTGCAGCTGGAGGCGAAGGTGAAGGAGCTGAGCGAGCGGCTGG AGGACGAGGAGGAAGTGAACGCGGACCTGGCCGCCCGCAGGCGCAAGCTGGAGGACGAGTGCACGGAGCT CAAGAAAGACATCGATGACCTGGAGCTGACGCTGGCCAAGGCCGAGAAAGAGAAACAAGCCACAGAGAAC AAGGTGAAGAACCTGACAGAAGAGATGGCGGCACTGGATGAAGCCGTGGTCCGCCTGACTAAGGAGAAGA AGGCATTGCAGGAGGCTCACCAGCAGGCTCTGGGTGACCTGCAGGCTGAGGAGGACCGTGTGAGCGCACT GGCCAAGGCCAAGATCCGGTTGGAACAGCAAGTGGAGGATCTGGAGTGCTCCCTGGAGCAAGAGAAGAAG CTGCGCATGGACACAGAACGCGCCAAGCGCAAACTTGAAGGAGACTTGAAGCTGACGCAGGAGACGGTGA CAGACACAACACAAGACAAGCAGCAGTTGGAGGAGAAGTTGAAGAAGAAGGATTCGGAGCTGAGTCAGCT GAACCTGCGGGTGGAGGATGAGCAGCTCGTGGGGGTGCAGCTGCAGAAGAAGATCAAGGAGCTGCAGGCT AGGGCCGAGGAGCTGGAGGAGGAGCTGGAGGCGGAGCGAGCAGCCCGCGCCCGCGTGGAAAAGCAGCGAG CCGAGGCAGCGCGGGAGCTGGAGGAGCTGAGCGAGAGGCTGGAGGAGGCTGGCGGAGCTTCCGCGGGGCA GCGTGAGGGTTGCCGCAAGCGTGAGGCTGAGTTGGGCCGGCTGCGGCGGGAGCTGGAGGAGGCAGTTCTG AGGCACGAGGCCACTGTGGCTGCCCTGCGCCGCAAGCAGGCCGATAGCGCTGCAGAGCTGAGCGAGCAGG TGGACAGCCTGCAGCGCATCCGGCAGAAACTGGAAAAGGAGAAGAGCGAGCTGCGCATGGAGGTGGACGA TCTGGGCGCCAGCGTGGAAACTCTGGCCCGTGGCAAGGCCAGTGCGGAAAAGCTGTGTCGGACCTATGAG GACCAGCTGAGCGAGGCCAAAATCAAAGTGGAGGAGCTGCAGCGACAGCTGGCCGACGCCAGCACCCAGC GGGGGCGGTTACAAACGGAGAATGGGGAGCTGGGCCGCCTGCTGGAGGAGAAGGAGTCCATGATCAGCCA GCTGAGCCGCGGGAAGACCTCAGCAGCGCAGAGTCTGGAGGAACTTCGGCGGCAGCTGGAGGAAGAAAGC AAGGCCAAGGGTGCGCTGGCTCATGCAGTGCAGGCCCTGCGGCACGACTGCGACCTCCTCCGAGAGCAGC ACGAGGAAGAGTCTGAGGCTCAGGCTGAGCTGCAGCGGCTGCTGTCCAAAGCCAACGCGGAGGTGGCCCA GTGGCGGAGCAAGTATGAAGCAGATGCCATCCAAAGGACGGAGGAGCTGGAGGAGGCCAAGAAGAAGCTG GCCCTGCGGCTGCAAGAGGCAGAAGAAGGCGTAGAGGCCGCAAATGCCAAATGCTCCTCACTGGAGAAGG CCAAGCTGCGGCTGCAGACAGAGTCGGAGGATGTGACCTTGGAGCTGGAGCGGGCCACCTCGGCCGCCGC AGCCCTGGATAAGAAGCAGCGGCACTTGGAGCGGGCGCTGGAGGAGCGGAGGCGGCAGGAGGAGGAGATG CAGCGGGAACTCGAGGCGGCACAGAGGGAGGCTCGAGGTCTGGGGACAGAGCTCTTTCGACTGCGACATA GCCACGAGGAAGCGCTTGAGGCTCTGGAGACACTCAAGCGGGAGAACAAGAATCTGCAGGAGGAGATCAG TGACCTTACAGACCAGGTCAGCCTCAGCGGGAAGAGCATCCAGGAACTGGAGAAGGCCAAGAAGGCGCTG GAAGGGGAGAAGAGTGAGCTGCAAGCTGCACTGGAGGAGGCTGAGGGGGCACTGGAGTTGGAAGAGACAA AGACTCTACGGATCCAGTTGGAGTTGTCCCAGGTCAAGGCAGAGGTGGACCGGAAGCTGGCGGAGAAGGA CGAGGAGTGTACTAACCTGAGGCGCAACCACCAGCGGGCTGTGGAATCCCTGCAGGCCTCCCTGGATGCA GAGACAAGGGCCCGCAATGAAGCACTGCGCCTCAAGAAGAAGATGGAGGGGGACCTCAACGACCTGGAGC TACAGCTGGGCCACGCAACCCGCCAGGCCATGGAGGCACAGGCAGCCACGAGGCTGCTGCAGGCTCAACT CAAGGAGGAACAAGCAGGGCGAGATGAGGAGCAGAGGCTGGCTGCCGAGCTCCGTGAGCAGGGTCAAGCC CTCGAGCGCAGGGCTGCACTGCTGGCGGCTGAGCTGGAAGAGCTTCGGGCTGCCCTGGAACAGGGCGAGC GCAGCCGGAGGCTGGCTGAGCAGGAGCTGCTGGAGGCCACCGAGCGCCTCAACCTACTGCATTCGCAGAA CACAGGCCTCCTGAACCAGAAAAAGAAGCTGGAGGTGGACTTGGCCCAGCTGAGTGGGGAAGTGGAGGAG GCTGCGCAGGAGAGGCGTGAAGCTGAGGAGAAGGCCAAAAAGGCCATCACTGATGCAGCCATGATGGCCG AGGAGCTGAAAAAGGAACAGGACACGAGCGCACACCTGGAACGGATGAAGAAGACCCTGGAACAGACCGT GCGGGAGCTGCAGGCACGCCTTGAGGAAGCCGAGCAGGCCGCACTTCGGGGTGGGAAGAAGCAAGTGCAG AAGCTGGAGGCCAAGGTGCGGGAGCTGGAGGCCGAGCTGGATGCAGAGCAGAAAAAGCATGCCGAGGCCC TCAAGGGGGTGCGTAAACACGAGCGCCGGGTCAAGGAGCTCGTGTACCAGACTGAAGAGGACAGGAAGAA CCTGGCTCGCATGCAGGACCTGGTGGACAAGCTGCAGAGCAAGGTCAAGAGCTACAAGCGTCAGTTTGAG GAGGCGGAGCAGCAGGCCAGTACCAACCTGGCTAAGTACCGCAAGGCCCAGCACGAGCTGGATGATGCCG AGGAACGGGCAGACATGGCAGAGACCCAGGCCAACAAGCTGCGGGCAAGGTCCAGGGATGCCCTGGGCCC CAAGCACAAGGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001085378
Insert Size	5826 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001085378.2, NP_001078847.1
RefSeq Size	6132 bp
RefSeq ORF	5826 bp
Locus ID	668940
UniProt ID	A2AQP0
Gene Summary	This gene encodes a myosin heavy chain. The encoded protein forms a hexamer comprised of two heavy chains, two alkali light chains, and two regulatory light chain components. This complex functions in muscle contraction. [provided by RefSeq, Jun 2013]

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