Thada (NM_183021) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	6530405K19; A930021O22; AU019899; BC052885; GITA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225066 representing NM_183021 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGTGAAGAAGAAAAGAGAAATGCAGGTTGCTGCGCTGACTGTCTGTCATCAGGACATGGAAACTT TGAGATCTTTTGCTGATATGGAAGGGAAAAATCTAGCCTCTTTGCTGTTACATTGTGTACAGCTCACAGA TGGAGTGTCACAAATCCACTCTATTAAACAGATTGTGCCTCTCTTGGAGAAGGTCGATAAAAACGGTGTG TGCGATCCTGCTATTCAGAGTTGTTTGGATATTCTAGCAGGCATTTATTTTTCCTTGACTCTGAAGAACC CCTTGAAGAAAGTTTTGGCCAGCTCACTCAATGGCCTACCTGAGGTTTTTCTAACTCAGGCAACACACAG TTTTACTTTTCATCTTCAAGAAGAATTAGATACTGCTGACTTATATTCTTATAGAAAAGTAATGGATAAC ATATCTTCCTGCATGGAGAACTTTAACCTGGGTAGAGCAAGTGTGGTTAATCTGCTTAAAGATGTGCTTC ATTTTCTGCAGAAGAGTTTAATTGAAATCCTGGAAGAAAACAGGAAGTTTGCTGGAAATCGTATTGTTCA AACACAGTTAATGAGTGACTTGCTGGTAGGTGTTAGAGTTGCCATGACATTGGTACAGAAAGTACAGGGG CCCCAGGGCAGCCTCTGGAATGACTCCAGCTCACCCATCTGGCAGAGTATGTGTGGCTTGCTGAGCATCT TCACCAAGTTCTTAAATGACGATGACCTACTGCAGACTGTTGAGAGTACATCTGGATTAGCTGTTATTCT TTTCATTAAGACCATGTTCCGGCCATCTGAAAAGCTTCCTGGATTGATCAGCAGCTTGCTTCTCCGGTCT GCTGAGTGCACTAGCATCCCTGAGTGGCTCATGAATAGCTGTAGGAGCCTTTGCTGTACTGATGTGCCAG CCTCAACTCTCTTATTCCTGTGTCAGGGAACACTTGCTATGCTGGACTGGCAGGATGGGAGCATGGGCCC CAGCGGGGAGGCTTTGCTCTTGGATATAGTGCATGTTTTGTTCACCTTGAGTTCACAGATTAAGGAGTCC ACATTAGATATGTTCCTGTCTAGAATCCTGGCATCTTGGACCAGTTCAGCCATACAGATCCTTGAATCAG GCTCCTCAGGCTTAAAGGGTCATCTGAACGGGGACTCATGCGTACCTAGGAGACTTCTGGAGTATGTCTA CACACACTGGGAGCACCCGTTAGACGCTCTGAGACATCAAACCAAAGTCATGTTCAGGAATCTCTTGCAA ATGCACCGGCTCACTATGGAAGGGGCAGATTTGGCCACTGACCCCTTCTGTCTGGAGTTGACTAAGAGTC TTCTGCAGCTGGAATGGCACATTAAAGGGAAGTACGCTTGCCTTGGCTGTTTGGTGGAGACTCTTGGAAT TGAACATATTTTGGCAATAGATAAAACCATTCCATCTCAAATTCTAGAGGTGATGGGAGACCAGTCATTG GTACCTTATGCAAGTGACCTCTTGGAAACCATGTTTAAAAATCATAAGAGCCATTTGAAGTCCCAAACTG TCACCAATACCTGGATGGACAAGTGGCATGAGACTTGGGTTTTCCCTGTCCTTTCTGTACTGTGTGGAGG AAACTTGGATCAGAGATCTTATGTGATTGATTATTACCTGCCACGGATACTGAATTACAGCCCCGAGAGC CTGCACTACATGGTGCACATTCTTCAGGCATCTACTGATACTGGAACTGGGTCTTGTAATCACAGAGGAG CTCTGGGAGCTTTGATGGCGTGTCTCCGAACAGCTAGAGCTCATGGACATCTTCAGTCTGCAACTCAGGC CTGGGAGAACCTTGTGTGCAGTGCAAGGGTAAAGCAAGGCTTAATTCACCAACACTGCCAAGTGCGGATA GATACACTAGGCTTGCTTTGTGAAAGTAATCGGAGTACAGAAGTGGTCTCTACAGAAGAAATGCAGTGGG TTCAGTTTTTTATCACTTACAACCTCAACAGCCAGTCTCCAGGGGTGCGCCAGCAGATTTGCTCACTCCT TAAAAAGTTGTTTTGCAGGATACAAGAAAGTTCACAGGTACTTTATAAATTGGAACAGAGAAAATCTACA CCTGACTCAGAGAACGGGTCCATCAGAGAGCAGCCTTCTGTCACTTTACAGCAGTATAAGAATTTCATGT CGTCTGTTTGCAACATTCTCTTTGAAGCATTGTTTCCTGGGTCTTCCTACTCGACTAGATTTTCAGCTTT AACTATCTTAGGATCAGTAGCTGAAGTGTTTCCTGATCCAGAAGGTAACATCCAAACAGTGTATCAGCTG AGTCATGATATTGATGCTGGCCGTTACCAAATACTTATGGAGTGTTTCACTAGTACTTTTGAAGAAGTGA AAACCTTAGCATTTGATCTTCTGATGAAGCTGTCGTCAGTAACAGCTGGACAGTTCCAGGATTCTGAGAA GCTGCAAGACCTGTTCCAGGCAGCGCTGGAACTCAGCACTAGCACCAAGCCTTATGATTGTGTGACAGCC TCCTACCTGCTGAACTTGCTGATACGGCAGGATGCTCTGCCAGCCGTCCTGTCTGCCTCTTCCCCACAGC AGCTCACACGTGGAGCAGGAGAGACGTCTGCTGTGCTGGAAAGGAACACATTAGTGGTTATTAAATGCTT GATGGAAAACCTTGAGGATGAAATATCTCAAGCTGAGAACTCTCTGCTTCAGGCTGCGTCTTCATTTCCA ATGTATGGGAGAGTCCACTGTATAACAAGAGCTTTTCAGAGGTTGCCTCTAAATGACCTGCGGCTGGCGA GCGAGTGGAGGCCGCTGCTGGGGAGGCTTCTGCTGCTGTCCTACAGGCTTTCCACTGTGGTGGCCCCTGT CATCCAGAGCTCCTCTCCTGAAGGCCTCATCCCTGTGGACACTGACTCAGCGTCAGCAAGCCGCTTGCAG CTCATTCTCAATGAGATCCAGCCACGGGATACTAACGATTACTTCAACCACACCAAAATACTGAAAGAAT GTGATAGCTTTGATTTGGAGGACTTGAGTACCAGTGTCTCAAATATTGATAGTTCTGCAGAAGTTAAAGG TAAAGAAGAAAAAGCGTGTGACGTAACTGCTCAGATGGTCCTCGCGTGCTGCTGGAGGAGCATGAAGGAA GTGGCTTTGCTTCTCGGCACGCTCTGCCAGCTTCTCCCTGTGCAGCCTGGGCCAGAATCTTCTAATGTGT TCCTAACAGTGCAGCAGGTTAAAGAAATAGGAGATTACTTTAAACAACACCTTTTGCAGTCCAGACACAG AGGAGCATTTGAACTGGCTTATACTGGTTTTGTGAAACTCACTGAAATATTGAACAGGTGCTCTAATGTG AGTCTGCAAAAGCTGCCAGAGCAGTGGCTGCGGAGCGTTCTGGAGGAAATTAAAGGCAGTGATCCCTCAT CCAAACTTTGTGCTACAAGGCGCAGTGCTGGGATTCCCTTCTACATACAGGCATTGTTGGCATCTGAACC AAAGAAGAGTAGAATGGACTTGCTGAAAATAACAATGAGGGAGCTGATCTCCTTGGCTTTGTCTGCAGAT GACTCAAAGGGCAGAGTCCCTCAGGTTCATGCTTTAAACATTCTCCGAGCCTTGTTCAGAGACACCCGTC TGGGAGAAAACATTATCCCCTATGTTGCTGGTGGAGCTAAGGCTGCAATTCTGGGCTTTACATCCCCTGT CTGGGCAGTGAGAAATTCATCCACACTTCTCTTTAGTTCCTTGATCACAAGAGTTTTTGGCGTTAAGAGA GGAAAGGATGAAGTTTCCAAAACAAATAGAATGACAGGAAGAGAGTTTTTCTCTCGTTTCCCAGAACTGT ACCCATTTCTTCTCAAACAGTTGGAAACTGTGGCCAGTACAGTAGACAGTGAACTGGGAGAGCCTGATCG CCACCCTGGCATGTTCCTCTTACTCCTGGTGTTGGAGAGACTGTACCCATCGCCGATGGACGGCACATCA TCTGCGCTCAGCTTGGCCCCTTTTGTTCCTTTCATTATCAGGTGTGGTCGCTCACCTATTTACCGCTCCC GGGAAATGGCAGCTCGTGCGTTGGTCCCCTTCATTATGATAGACCAAATACCGAGTACCCTCTGTGCTCT ACTGAACTCACTCCCCAACAGCACTGACCAGTGTTTCCGACAAAACCACATTCATGGGACACTTCTGCAA GTTTTTCATCTGTTACAAGCCTACATTACAGACTGCAGACACAGAACAAATGCAGATTTCCTGCAAGAGC TGAGTGATGTCACTGCTTGTACCAAAGCCAAACTCTGGCTGGCCATGAGGCAAAATCCATGCCTGGTAAC CAGAGCTGTGTATATTGATATTCTCTTCCTGCTGACTAACTGCCTGGACAGACCTGAGGAAGGCAAGCAG ACAGCTCTGGAGAGTCTTGGCTTCTGGGAAGATGTGAGAAGAATTATCCTGGAATCAGAGCTGATTAAGG GGTTCCCCTGGACCTTCAAGGTGCCAGGCTTGCCCCAGTATCTGCAGAGCCTTACCAAACTAGCCATCCC TGAAGTGTGGGCGTCGCTTGCTGAAGCTAAAGGGCAGGCGACTGCTGTTCCTCTCTCCTTCTCTCGGCTT CTGAAGTCTTCCTTTCCTGAAGTGCGCTTGCTAGCCCTAGACACCTTGCTGGAAAGGGCAAGGTCCTCTG AACAGGAGCAGAAGGAGCCACTTCCTCTGCTCTGCAGTATGGGAGAGGAGCTTCTACTGCTGGCGATGAA GGAAGACCACCCCGGATGTTTCTGCAGGGTCCTAAAGATTCTCTATCACTTGAACCCCAGCGAATGGCTT CCCCAGACAGAGTGCTATGCTCACCTCTCCCCAAAGGAGTTCCTCATGTGGAGTATGGATATTGCTTCCA ATGACAGATCTGAAATCCAGGGAGTAGCTCTGAAGCTTGCCTCTAAAATAATTGCCTACCGTGTACAGAG TTGCGAGAAGAACAAAGATTCACTCGCCCCTGAGCTGAGGCAGTGGGTCCAGCTGGTTGTCTGGTCCTGT GGAGACCATCTTCCTACTGCATCTAGACTGGCTGTTGCTGAAGTCCTCACAAGCACCGCACCCCTGTTCC TCACCAGTCCCCAGCCCATTCTCGAACTGCAGGGCACACTTTCTCTCTGGAGGTGTGTTCTTACCCTTCT GCAGAGTGAGGAGCAAACCGTCAGAGAGGCAGCCACAGAAATCGTGACAACAGCCATGTCACAAGGAAAC ACTTGCCAGTCAACAGAATTTGCCTTCTGCCAGGTGGATGCCTCCATTGCTCTGACCCTGGCCTTAGCTG TTCTGTGTGACTTGCTCCAGCAGTGGGACCAGCTGGAGCCAGGACTGCCTATTCTACTGGGATGGCTACT GGAAGAGGGTGATGACCTCGAGGGCCACGTGCAGAGCCCACATCAGGGGGAAGAAGAGCATATCTTTGAA AAATCAGAAGTCAACTTCTGGGCTGAGACTCTGACCTTCGTGAAATCCCTGTGTAGGCAACTCTTCCATC TCCTCTGTCAATCTGGCTGGCAGTCCCCTCACTCCCAGAAGCTCTGTCACTTGCAAAGGATAGCATCAGA GCAGAGTCACCTCATCTCTCAGCTCTTCAGAGAACTTCCACTCTCAGCTGAGTTTTTGAAGACAGTGGAA TACACGAGGCTGCGCATTCAGGAGGAAAGGACTTTGGCTGTCCTGAGGCTGTTGGCCTGCCTGGAAGGAA AGGAGGGTCTCAGAGCCGAAGACTGCCCTAGGGAATGGAGACAAGTAATGGCCCCAAGAACAGAAGCAGC ATGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_183021
Insert Size	5817 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_183021.3, NP_898842.2
RefSeq Size	7896 bp
RefSeq ORF	5817 bp
Locus ID	240174
UniProt ID	A8C756

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