Myh3 (NM_001099635) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	MyHC-emb; Myhs; Myhs-; Myhs-e; Myhse
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225061 representing NM_001099635 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTAGCGACACCGAGATGGAAGTGTTTGGCATAGCTGCACCTTTCCTCCGCAAGTCGGAAAAGGAGA GGATCGAAGCTCAGAACCAGCCCTTTGATGCCAAAACCTACTGCTTTGTGGTTGACTCAAAGGAAGAATA TGTCAAGGGGAAAATTAAGAGTAGCCAGGATGGGAAAGTCACTGTGGAGACAGAAGACAGCAGGACCCTG GTGGTTAAGCCAGAGGACGTGTATGCCATGAACCCACCCAAGTTTGACAAGATTGAGGACATGGCCATGC TGACCCACCTGAACGAGCCCGCTGTGCTGTACAACCTCAAGGACCGATACACGTCCTGGATGATCTACAC CTATTCAGGCCTCTTCTGTGTCACTGTCAACCCCTACAAATGGCTGCCAGTGTACAACCCCGAGGTGGTG GACGGCTACCGAGGCAAAAAACGCCAGGAGGCTCCGCCCCACATCTTCTCCATCTCCGACAACGCCTACC AGTTCATGCTGACTGATCGTGAGAACCAGTCTATCCTGATCACCGGAGAATCCGGGGCCGGGAAGACGGT GAACACCAAGCGGGTCATCCAGTACTTTGCAACAATTGCAGCCACTGGGGACCTTGCCAAGAAGAAGGAC TCCAAAATGAAGGGGACGCTGGAGGATCAAATTATCAGCGCTAACCCCCTGCTGGAGGCCTTTGGGAACG CCAAGACCGTGAGGAACGACAACTCGTCTCGCTTTGGCAAGTTCATCCGCATCCATTTTGGCACCACTGG GAAGCTGGCTTCTGCAGATATTGAAACTTATCTGCTGGAAAAGTCCAGAGTCACCTTCCAGCTGAAGGCT GAGAGAAGCTACCACATCTTCTACCAGATTCTCTCCAACAAGAAACCTGAGCTGATTGAGCTGCTGCTGA TTACGACCAACCCTTATGACTACCCGTTCATCAGCCAGGGCGAAATCCTGGTGGCCAGCATCGACGATGC GGAGGAGCTGTTAGCTACGGATAGTGCCATCGACATCCTGGGCTTCACCCCGGAGGAGAAATCTGGACTT TATAAACTGACAGGGGCTGTGATGCATTATGGGAACATGAAGTTCAAGCAGAAGCAGCGGGAGGAGCAGG CCGAGCCAGATGGCACTGAAGTGGCTGACAAAACAGCCTACTTGATGGGCCTGAATTCCTCAGACCTCCT CAAGGCCTTGTGCTTTCCCAGAGTGAAAGTCGGGAATGAATACGTTACAAAAGGGCAGACTGTGGACCAG GTTCACCATGCTGTGAACGCTCTCTCCAAGTCCGTGTACGAGAAGCTGTTCTTGTGGATGGTCACTCGTA TTAACCAGCAGCTGGATACAAAGCTACCAAGACAGCACTTCATTGGCGTTTTGGACATTGCGGGTTTTGA AATTTTTGAGTATAACAGCCTGGAGCAGCTGTGCATCAACTTCACCAACGAGAAACTGCAACAGTTTTTC AACCACCACATGTTCGTGCTGGAACAGGAGGAATACAAGAAGGAGGGCATCGAGTGGACCTTCATCGACT TCGGGATGGACCTGGCAGCCTGCATCGAGCTCATCGAGAAGCCGATGGGCATCTTCTCCATCCTGGAAGA GGAGTGCATGTTCCCCAAGGCGACAGACACCTCCTTCAAGAACAAGCTGTATGACCAGCACCTGGGAAAG TCCAACAACTTCCAGAAACCTAAGGTGGTCAAAGGCAAGGCTGAAGCCCACTTCTCCCTGGTGCACTATG CGGGCACCGTGGACTACAGTGTCTCAGGCTGGCTGGAGAAGAACAAGGACCCCCTGAATGAGACCGTGGT GGGACTGTACCAGAAGTCCTCCAACAGACTCCTGGCACATCTCTATGCCACCTTCGCTACAACAGATGCG GACGGTGGGAAGAAGAAAGTCGCTAAGAAGAAAGGCTCTTCCTTCCAAACCGTCTCTGCACTGTTCAGGG AAAACTTGAACAAGCTGATGTCCAATTTAAGAACCACACACCCACACTTTGTACGCTGTATAATACCCAA CGAAACCAAGACCCCAGGGGCCATGGAACACAGCCTTGTCCTGCACCAGCTCAGGTGTAACGGGGTGCTG GAAGGCATCCGGATCTGCAGGAAGGGCTTCCCCAACAGGATCCTGTATGGAGACTTTAAACAGAGATACC GAGTGCTGAACGCCAGTGCCATCCCAGAAGGCCAGTTCATTGACAGCAAGAAAGCTTGCGAGAAACTCTT GGCTTCCATTGACATCGACCACACACAGTACAAATTTGGACACACCAAGGTGTTCTTTAAGGCTGGCTTG CTAGGAACCCTGGAAGAGATGCGGGATGAGCGTTTGGCCAAACTGATCACTCGGACACAAGCCGTGTGCA GAGGGTTTCTCATGCGTGTGGAATTCCAGAAGATGATGCAGAGGAGGGAGTCCATCTTCTGCATCCAGTA CAACATCCGTGCCTTCATGAACGTCAAGCACTGGCCCTGGATGAAACTCTTCTTCAAGATCAAGCCCCTG CTGAAGAGCGCGGAGACTGAGAAGGAGATGGCCACCATGAAGGAGGAGTTCCAGAAAACCAAGGATGAGC TGGCTAAGTCGGAGGCAAAGAGGAAGGAGCTAGAGGAGAAGCTCGTCACTTTGGTACAGGAGAAGAATGA CCTGCAACTTCAAGTACAGGCTGAGAGTGAAAATTTGTTGGATGCTGAGGAACGATGCGACCAGCTGATC AAAGCCAAGTTCCAGCTGGAAGCCAAAATCAAAGAGGTGACAGAGAGAGCCGAGGACGAGGAGGAGATCA ACGCGGAGCTCACGGCCAAGAAGAGGAAGCTGGAGGACGAGTGCTCGGAGCTGAAGAAGGACATCGATGA CCTTGAGCTGACACTGGCCAAGGTTGAGAAGGAGAAGCACGCCACAGAGAACAAGGTCAAAAACCTTACC GAAGAACTGGCAGGCCTGGATGAAACTATTGCAAAGCTAACACGGGAGAAGAAGGCCCTCCAAGAGGCCC ACCAGCAGACCCTGGACGACCTCCAGGCTGAAGAAGACAAGGTCAATTCTCTGAGCAAGCTCAAGAGCAA GCTGGAGCAGCAAGTGGATGATCTGGAGAGCTCCCTCGAACAAGAAAAGAAGCTGCGTGTGGACCTAGAA AGGAACAAAAGGAAGCTGGAGGGAGACCTGAAGCTTGCCCAAGAGTCCATATTAGATCTGGAGAACGACA AGCAACAGCTAGACGAGAGGCTCAAGAAGAAGGACTTTGAATATAGTCAGCTGCAAAGCAAAGTGGAAGA TGAGCAGACCTTGAGCCTCCAGCTTCAGAAGAAAATCAAGGAGCTACAGGCTCGCATCGAGGAGCTGGAG GAAGAGATAGAGGCAGAGAGGGCCACCCGGGCCAAGACAGAGAAGCAGCGCAGCGACTATGCACGCGAGC TGGAGGAGCTGAGTGAGCGGCTGGAGGAGGCGGGCGGTGTCACCTCCACCCAGATCGAACTGAACAAGAA GCGTGAGGCTGAGTTCTTGAAGCTGCGCAGGGACCTGGAGGAGGCCACCCTGCAGCACGAGGCCACGGTG GCCACGCTGCGGAAGAAGCACGCGGACAGCGCGGCCGAGCTGGCGGAGCAGATCGACAACCTGCAGCGGG TGAAGCAGAAGCTGGAGAAGGAGAAGAGCGAGTTCAAGCTGGAGATTGACGACCTCTCCAGCAGCGTAGA GAGCGTGTCCAAATCCAAGGCCAATCTGGAGAAGATCTGCCGAACTCTAGAGGATCAGTTAAGTGAGGCC AGGGGCAAGAATGAGGAGATGCAGAGGAGCCTAAGTGAGCTGACCACACAGAAGTCCCGGCTGCAGACAG AGGCGGGTGAGCTGAGTCGTCAACTAGAAGAAAAGGAAAGCATTGTATCTCAGCTCTCCAGGAGCAAGCA GGCCTTTACCCAACAGATAGAGGAGCTCAAGAGGCAACTGGAGGAAGAGAACAAGGCCAAGAACGCGCTG GCCCACGCCCTGCAGTCCTCCCGCCATGACTGTGACCTGCTGCGGGAACAGTACGAGGAGGAGCAGGAAG GCAAAGCCGAGCTGCAGAGGGCACTGTCCAAGGCCAACAGCGAGGTGGCCCAGTGGAGGACCAAGTATGA AACGGATGCCATCCAGCGCACGGAGGAGCTGGAGGAGGCCAAAAAAAAGCTTGCGCAGCGCCTTCAGGAT TCGGAGGAGCAGGTAGAGGCTGTAAATGCCAAATGTGCTTCCTTGGAAAAGACCAAGCAGAGGCTGCAAG GGGAGGTGGAGGACCTGATGGTGGATGTGGAAAGAGCCAACTCCCTGGCTGCCGCTCTGGACAAGAAGCA GAGGAACTTTGACAAGGTGCTAGCTGAGTGGAAGACGAAGTGTGAGGAGAGCCAAGCCGAGCTGGAGGCA GCTCTCAAGGAATCCCGGTCCTTGAGCACTGAGCTCTTCAAACTGAAGAATGCCTATGAAGAAGCCTTGG ATCAACTTGAAACCGTGAAACGGGAGAATAAGAACTTAGAACAAGAGATAGCAGATCTCACTGAACAGAT TGCCGAGAACGGTAAAAGCATCCATGAGCTAGAGAAATCCAGAAAACAGATGGAGCTGGAGAAGGCTGAC ATCCAGATGGCGCTGGAGGAAGCAGAGGCAGCTCTTGAGCACGAAGAAGCCAAGATTCTCCGGATCCAGC TTGAACTGACCCAGGTGAAATCAGAGATTGATAGGAAGATTGCAGAGAAGGATGAAGAGATCGAGCAGCT GAAGAGGAACTACCAGAGGACAGTGGAGACCATGCAAGGCGCCCTGGACGCTGAGGTGCGCAGCAGGAAT GAGGCCATCCGCCTGAAGAAGAAGATGGAGGGCGACCTCAATGAGATCGAGATCCAGCTGAGCCACGCCA ACCGCCAGGCCGCAGAGACCATCAAGCACCTCCGGAGCGTCCAGGGACAGCTGAAGGACACCCAGCTGCA CCTGGATGACGCTCTCCGGGGCCAGGAGGACCTGAAGGAGCAGCTGGCCATCGTGGAACGCAGAGCCAAC CTCCTGCAGGCTGAGGTGGAGGAGCTGCGGGCCACGCTGGAGCAGACGGAGAGGGCCCGGAAGCTGGCAG AGCAGGAGCTGTTGGATTCGAATGAGAGGGTCCAGCTGCTGCACACCCAGAACACAAGCCTCATCCACAC CAAGAAGAAGTTGGAGACTGACCTCACGCAGCTCCAGAGTGAGGTGGAGGATGCCTGCAGGGACGCAAGG AATGCAGAGGAGAAGGCCAAAAAGGCCATCACTGACGCCGCCATGATGGCGGAGGAGCTGAAGAAGGAGC AGGACACCAGCGCCCACCTGGAGAGGATGAAGAAGAACCTGGAGCAGACGGTGAAGGACCTGCAGCACCG TCTGGATGAGGCTGAGCAGCTGGCGCTGAAGGGCGGCAAGAAGCAGATTCAGAAACTGGAGACACGGATC AGAGAGCTGGAGTTTGAGCTGGAAGGGGAGCAGAAGAGGAACACAGAGTCTGTGAAGGGCCTGAGGAAGT ATGAGCGGCGTGTTAAGGAGCTCACATATCAGAGTGAGGAGGACAGGAAGAATGTGTTGAGATTGCAGGA TCTGGTGGATAAACTCCAAGTGAAAGTCAAGTCCTACAAGAGGCAGGCTGAGGAGGCTGATGAACAAGCC AATGCTCATCTCACCAAGTTCAGGAAAGCCCAGCATGAGCTAGAGGAGGCCGAGGAACGGGCTGATATCG CAGAATCGCAAGTCAATAAACTGCGGGCAAAGACCCGTGACTTCACCTCTAGCCGGATGGTGGTCCATGA AAGCGAGGAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001099635
Insert Size	5823 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001099635.1, NP_001093105.1
RefSeq Size	5992 bp
RefSeq ORF	5823 bp
Locus ID	17883
UniProt ID	P13541
Gene Summary	Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. This gene is a member of the MYH family and encodes a protein with an IQ domain and a myosin head-like domain. [provided by RefSeq, Sep 2015]

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