Myh2 (NM_001039545) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	MHC2A; Myh2a; MyHC-IIa; Myhs-f; Myhs-f1; Myhsf1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225060 representing NM_001039545 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTCCGACGCCGAGATGGCCGTTTTCGGGGAGGCTGCCCCTTACCTCCGGAAGTCCGAAAAGGAGC GAATCGAGGCCCAGAATAGGCCTTTTGATGCCAAAACATCTGTCTTTGTGGCGGAGCCCAAGGAATCCTT TGTCAAAGGAACCATTCAGAGCAAAGATGCAGGGAAAGTGACTGTGAAAACAGAAGCAGGAGCGACCCTG ACCGTGAAAGAAGACCAGATCTTCCCCATGAACCCTCCCAAGTACGACAAGATCGAGGACATGGCCATGA TGACCCACCTGCACGAGCCCGCTGTGCTGTACAACCTCAAAGAGCGTTATGCAGCCTGGATGATCTACAC CTACTCAGGCCTCTTCTGTGTCACCGTCAACCCTTACAAATGGCTGCCGGTGTACAACCCCGAGGTGGTG GCGGCCTATCGAGGCAAAAAGCGCCAGGAGGCCCCGCCCCACATCTTCTCCATCTCTGATAACGCCTACC AGTTCATGCTAACAGACAGGGAGAATCAGTCAATCCTGATCACCGGAGAATCCGGGGCCGGGAAGACTGT GAACACGAAGCGTGTCATCCAGTACTTTGCAACAATTGCAGTCACTGGGGACAAGAAGAAGGAGGAGGCA ACTTCTGGCAAAATGCAGGGGACGCTGGAGGACCAAATCATCAGTGCCAACCCCCTGCTGGAGGCCTTTG GGAACGCCAAGACCGTGAGGAATGACAACTCGTCTCGCTTTGGTAAATTCATCAGGATCCACTTTGGCAC TACGGGGAAACTGGCATCTGCTGACATCGAGACCTATCTGCTAGAGAAGTCCCGGGTCACTTTCCAGCTT AAGGCGGAAAGAAGCTACCATATATTTTATCAGATCACATCCAACAAGAAGCCAGAGCTGATCGAAATGC TGCTGATCACCACGAACCCATATGATTACCCATTTGTCAGCCAGGGGGAGATCAGTGTGGCCAGCATTGA TGACCAGGAAGAGCTGATGGCCACTGATAGTGCTATCGACATTTTGGGCTTTACAAATGATGAAAAAGTC TCCATCTATAAGCTCACCGGTGCTGTGATGCATTATGGGAACATGAAGTTCAAGCAGAAGCAGCGGGAGG AGCAGGCAGAGCCAGATGGCACCGAAGTTGCTGACAAAGCTGCCTATCTTCAGGGTCTGAACTCTGCTGA CCTGCTCAAAGCCCTCTGCTACCCCAGGGTCAAGGTCGGCAATGAGTACGTCACCAAAGGCCAGACTGTC GAGCAGGTGACCAATGCTGTGGGTGCCCTGGCCAAGGCCATGTATGAGAAGATGTTCCTGTGGATGGTCA CCCGCATCAACCAGCAGCTGGACACCAAGCAGCCCCGGCAGTACTTCATCGGGGTCTTGGACATCGCTGG CTTTGAGATCTTTGATTTCAACAGCCTGGAACAGCTGTGCATCAACTTCACCAATGAGAAACTGCAACAG TTTTTCAACCACCACATGTTCGTGCTGGAGCAGGAGGAATACAAGAAGGAGGGCATCGAGTGGACCTTCA TCGACTTCGGGATGGACCTGGCGGCCTGCATCGAGCTCATCGAGAAGCCGATGGGCATCTTCTCCATCCT GGAAGAGGAGTGCATGTTCCCTAAGGCGACAGACACCTCCTTCAAGAACAAGCTGTATGAGCAGCATCTT GGAAAGTCTGCCAACTTCCAGAAGCCTAAGGTGGTCAAAGGCAAGGCCGAAGCCCACTTCTCCCTCATCC ACTATGCGGGCACCGTGGATTACAACATCACTGGCTGGCTGGACAAGAACAAGGACCCACTGAACGAGAC CGTGGTGGGGCTGTACCAGAAGTCTTCAGTGAAAACTCTGGCTTATCTCTTCTCTGGGGCACAAACTGCT GAAGCAGAGGCAAGTAGTGGTGGAGCTGCCAAGAAAGGTGCCAAGAAGAAGGGGTCCTCTTTTCAGACCG TGTCTGCCCTGTTCAGAGAGAATTTGAACAAGCTGATGACCAATCTCAGGAGCACCCATCCTCATTTCGT GAGGTGTATCATCCCCAATGAAACCAAGACTCCCGGTGCCATGGAGCACGAACTGGTCCTGCACCAGCTG AGGTGTAACGGGGTGCTGGAGGGCATCCGCATCTGCAGAAAGGGCTTCCCCAGCAGGATCCTCTATGCAG ACTTCAAGCAGAGATACAAAGTGTTAAACGCAAGTGCCATTCCTGAGGGTCAGTACATCGATAGCAAGAA GGCTTCTGAGAAGCTCCTGGGCTCCATTGACATTGACCACACTCAGTATAAGTTTGGTCACACCAAGGTC TTTTTCAAAGCTGGTCTTCTGGGGCTCCTAGAGGAGATGAGAGATGACAAGTTGGCCCAGCTTATTACCC GGACCCAGGCTATGTGCAGAGGGTTCTTGGCGAGAGTGGAGTACCAGAAGATGGTGGAGAGAAGGGAGTC CATCTTCTGCATCCAGTACAACATCCGCGCCTTCATGAACGTCAAGCACTGGCCCTGGATGAAACTCTTC TTCAAGATCAAACCCCTGCTGAAGAGCGCAGAGACCGAGAAGGAGATGGCCACCATGAAGGAGGAGTTCC AGAAAACCAAAGATGACCTTGCCAAGTCAGAGGCAAAGAGGAAGGAACTTGAAGAAAAGATGGTGTCCCT CTTGAAAGAAAAAAATGACTTGCAACTTCAAGTTCAGGCTGAAGCTGAAGGCTTGGCCGATGCAGAGGAG CGATGCGACCAGCTGATCAAAACCAAAATCCAGCTGGAGGCCAAAATCAAAGAGGTGACCGAGAGGGCCG AGGATGAGGAGGAGATCAACGCAGAGCTCACGGCCAAGAAGAGGAAGCTGGAGGACGAGTGCTCAGAGCT GAAGAAGGACATCGATGACCTTGAGCTGACACTGGCCAAGGTTGAGAAGGAGAAGCACGCCACGGAGAAC AAGGTGAAAAACCTGACAGAGGAGATGGCAGGCCTGGACGAAACCATCGCCAAGCTGACCAAGGAGAAGA AGGCCCTCCAGGAGGCCCACCAGCAGACCCTGGATGACCTGCAGGCAGAGGAGGACAAAGTCAACACCCT GACCAAAGCCAAAATCAAGCTGGAACAGCAAGTGGATGACCTTGAAGGGTCCTTGGAGCAAGAAAAGAAA CTTCGCATGGACCTAGAAAGAGCCAAGAGAAAACTTGAGGGCGACTTGAAGTTAGCCCAGGAGTCCATCA TGGACATTGAAAATGAGAAACAGCAGCTCGATGAAAGGCTCAAAAAGAAAGAGTTTGAAATGAGCAATCT GCAGAGCAAGATTGAGGACGAGCAGGCCATCGGCATTCAGCTGCAGAAGAAGATCAAGGAGTTGCAGGCC CGCATCGAGGAGCTGGAGGAGGAAATCGAGGCAGAGCGGGCCTCCAGGGCCAAAGCAGAGAAGCAGCGCT CTGACCTCTCCCGGGAACTGGAGGAGATCAGCGAGAGGCTGGAAGAAGCCGGCGGAGCCACTTCAGCCCA GATCGAGATGAACAAGAAGAGAGAGGCTGAGTTCCAGAAAATGCGCAGGGACCTGGAGGAGGCCACGCTG CAGCATGAAGCCACAGCAGCCACCCTGAGGAAGAAGCATGCGGACAGCGTGGCCGAGCTCGGGGAGCAGA TCGACAACCTGCAGCGGGTGAAGCAGAAGCTGGAGAAGGAGAAGAGCGAGATGAAGATGGAGATCGATGA TCTTGCCAGTAATGTAGAGACAGTGTCTAAGGCCAAGGGAAACCTCGAGAAGATGTGCCGCACCCTGGAG GACCAGGTGAGTGAGCTGAAGTCAAAGGAGGAGGAACAGCAGCGACTGATCAACGACCTGACAACCCAGA GAGGACGACTGCAGACCGAATCCGGTGAATTTTCCAGGCAGCTTGATGAGAAGGAAGCGCTGGTATCTCA GTTATCAAGGGGGAAGCAAGCATTCACTCAACAGATTGAGGAGCTAAAGAGGCAGCTTGAAGAGGAAGTA AAGGCCAAGAACGCGCTGGCCCACGCCCTGCAGTCCTCCCGCCATGACTGTGACCTGCTGCGGGAACAGT ACGAGGAGGAGCAGGAGTCTAAGGCTGAACTGCAGAGGGCGCTGTCCAAGGCCAACAGCGAGGTGGCCCA GTGGAGGACCAAATATGAGACGGATGCCATCCAGCGCACGGAGGAGCTGGAGGAAGCCAAGAAGAAGCTG GCTCAGCGTCTGCAGGCGGCTGAGGAGCACGTAGAAGCCGTGAACGCCAAGTGCGCTTCTCTGGAGAAGA CGAAGCAGCGGCTGCAGAACGAGGTGGAGGACCTCATGCTGGATGTGGAGAGGACCAACGCTGCTTGTGC CGCCCTGGACAAGAAGCAGAGAAACTTCGACAAGATCCTGGCAGAGTGGAAGCAGAAGTATGAGGAAACC CACGCTGAGCTCGAGGCATCCCAGAAGGAGGCCCGCTCCCTGGGCACTGAGCTCTTCAAGATGAAGAATG CCTACGAGGAGTCTCTGGATCAGCTAGAAACCCTGAAGCGAGAGAATAAGAACTTACAGCAGGAGATTTC TGACCTCACGGAACAGATTGCAGAAGGAGGAAAGCGCATCCACGAACTGGAGAAAATTAAGAAACAAGTC GAACAAGAGAAGTGTGAACTTCAGGCTGCTCTAGAAGAAGCAGAGGCATCTCTGGAGCACGAGGAGGGAA AGATCCTGCGCATCCAGCTGGAGCTGAACCAAGTCAAGTCTGAGATCGACAGGAAGATTGCTGAGAAGGA TGAGGAGATCGACCAGCTGAAGAGAAACCACATTAGAGTCGTGGAGTCCATGCAGAGCACGCTGGATGCC GAGATCAGGAGCAGGAACGACGCCATCAGAATCAAGAAGAAGATGGAGGGAGACCTGAACGAGATGGAAA TCCAGCTGAACCACTCCAACCGCATGGCTGCCGAGGCCCTGAGAAACTACAGGAACACACAAGGCATCCT CAAGGACACCCAGTTGCACCTGGACGATGCTCTCCGGGGCCAGGAGGACCTGAAGGAGCAGCTGGCCATG GTTGAGCGCAGAGCCAACCTGCTGCAGGCTGAGATCGAGGAGCTGCGGGCCACGCTGGAGCAGACGGAGA GGAGCAGGAAGATTGCAGAGCAGGAGCTGCTGGACGCCAGTGAGCGCGTGCAGCTCCTCCACACCCAGAA CACCAGCCTCATCAACACCAAGAAGAAGCTGGAGACAGACATTTCCCAAATCCAGGGAGAGATGGAGGAC ATCGTCCAGGAAGCCCGCAATGCAGAAGAGAAAGCTAAGAAAGCCATCACTGATGCCGCCATGATGGCGG AGGAGCTGAAGAAGGAGCAGGACACTAGCGCCCACCTGGAGCGGATGAAGAAGAACATGGAGCAGACCGT GAAGGACCTGCAGCTGCGTCTGGATGAGGCTGAGCAGCTGGCGCTGAAGGGCGGCAAGAAGCAGATCCAG AAACTGGAGGCCAGGGTGCGTGAACTGGAGGGTGAGGTAGAGAGTGAGCAGAAGCGGAATGCTGAGGCCG TCAAAGGTCTGCGCAAACACGAGAGACGAGTGAAGGAGCTTACTTACCAGACAGAAGAAGACCGAAAAAA TATTCTCAGGCTTCAGGATTTGGTGGATAAACTCCAGGCAAAAGTGAAATCTTACAAGAGACAAGCTGAG GAGGCTGAGGAACAATCCAACACAAATCTATCCAAGTTCCGCAAGATCCAGCACGAGCTGGAGGAAGCCG AGGAGCGGGCTGACATCGCGGAGTCCCAGGTCAACAAGCTGCGGGTGAAGAGCCGGGAGGTTCACACCAA AATCATAAGCGAAGAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001039545
Insert Size	5829 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001039545.2, NP_001034634.2
RefSeq Size	6083 bp
RefSeq ORF	5829 bp
Locus ID	17882

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