Myh15 (NM_001166210) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	EG667772
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225048 representing NM_001166210 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCTGTCTGAGTTTGGAGATGCCACAGCCTTCCTCAGAAGAAATAAAGCTGACCCTCTGTTCCAGC CACCAGCCTTTGATGGGAAGAAGAAATGCTGGGTTCCCGATGGGAAGAATGCTTATATTGAGGCTGAGGT TAAAGAAAGCGGTGATGATGGACAAGTAATTGTAGAGACTAGAGATGGAGAGATTATGAGAATTAAGGAG GACAAACTTCAACAGATGAACCCTGAAGAGCTTGAAATGATAGAAGATCTGTCAATGCTGCTTTACCTCA ATGAAGCATCTGTACTGCACACCCTGAGGAGGCGATATGACCATTGGATGATCTATACATATTCAGGTAT CTTCTGTGTGGCTATAAACCCTTACAAGTGGCTCCCTGTGTATCAGAAAGAGGTCATGGCTGCCTATAAA AGGAAGAGGCGATCCGAGGTTCCCCCTCACATCTTTGCTGTGGCCAATCGTGCCTTTCAGGATATGCTTC GCAATGGAGAGAACCAGTCTATAGTCTTCACAGGAGAATCTGGTTCTGGAAAGACAGTGAACACCAAACT GATCATTCAGTATTTTGCCACCATGGCAGCCATAAGTGAGCCTAAGAAAAAGCTGGGGAATTTAGAAGAT CAAATTGTAAAAATGAATCCTCTCTTAGAAGCATTTGGAAATGCCAAAACTCAGAAAAATGACAACTCTT CGCGTTTTGGAAAACTCATCCGGATACATTTTGGTGCAAGAGGGACACTGTCATTTGCAGATATTCAGAT CTATTTTCTTGAAAAATCTCGAGTGGTTTATCAGCAGCCTGGAGAGAGGAACTACCATATCTTTTACCAA ATTCTGTCTGGAAATCAAGAACTTCGGAACATGCTTCTGGTGTCCACAAATCCCTCTGACTTTCATATTT GCTCCTGTGGAGTGGTTGCTGTGGAGAGCCTGGATGATGCTAAAGAATTTCTGGCCACAGAAAAAGCCAT AGATGTCTTGGGATTTCTACCTGATGAGAAGTTTGGGTGCTATAAGCTCGTTGGAGCCATCATGCACTTT GGAAACCTGAAGTTTAAACGGAATCTCAGAGAGGAACAACTGGAGGCTGATGGCACAGAAAATGCTGACA AAGCTGCTTTCCTCATGGGAATTCACGCTTCTGAGTTGTTAAAAGGCCTAATATATCCTAGAATCAAAGT TGGTAATGAATATGTTACCAGAAGTCAAAACCTACAACAGGTAACCTATGCAGTCGGTGCACTGTCTCAG TCAATATATGAGAGGATGTTTCAGTGGCTAGTGGCACGCATGAACCAAGTCCTGGACGCCAAGCTGACAA GTCACTTCTTCGTTGGGATTCTTGACACCACTGGTTTTGAAATACTTGATTATAATAGTCTTGAGCAACT CTGCATCAATTTTACCAATGAAAAGTTACAACAGTTTTTCAATCAGCAACTGTTTATTCTGGAGCAAGAA GAATACAGGAAAGAGGGCCTTGACTGGCTGTCCATTGACTATGGTCTGGATGTGCAAGCCTGCATAGATT TCATTGAGAAGCCCATGGGCATCTTTTCCATCCTTGAAGAGGAATGTATGCTTCCTAAAGCTACTGACCA GATGTTCAAGACCAAACTCTTTGACCACCATTTTGGGAAGTCAGCTTACTTCCAGACACCCACATCTCCT GAGAAGAACTTTGAAGTTCACTTTGAACTTGCTCATTATGCAGGAGTGGTCCCTTATAATATCAGTGGTT GGATTGGAAAGAACAAAGGGCTTCTTAATGAAACAGTGGTGGCTCTACTTCAGAAGTCTTCCAACAAAGT CCTGGCGAATCTCTTTACAAAGGACATCATTGCTGGTAGTGCTTCACAGTTTGGTGAGAAGACACACAAG AAAGGAACATCTTTCCACTTGATTACATCTCTTCATAAAGAGAACATAAATAAATTGATGACAGATTTGA AATCAACAGCACCTCATTTTGTGAGATGTATAAATCCTAATAAGAACAAAATTCCAGGTGTAATGGATCC TTTCTTGGTTCTCCAGCAGCTGCGCTGTAATGGTGTCTTAGAAGGCATTAGAGTATGCTGTGAAGCATTC CCAAGTTGGATGCTATATGATGACTTTAAGCAAAGGTACTGGATTCTAAACCCAAGAATCTTTTCAAAGA GCAAGTTTGTGAGCAGCAGAAAAGCAACTGAAGAAGTGCTTGACTTCTTGGAGATAGACCATCCCCATTA CCAGTGTGGAGTCACCAAGGTGTTTTTTAAAGCTTTCATCCTGGACCAGTTAGAAGAAAGAAGGGATGAA AAAATATCTAAAGTCTTCACTTTGTTCCAAGCCAGAGCACGAGGAAAACTTATGCGCATCACATTCCAGA AGATTCTAGAAGAAAGGGATGCCCTTGCTTTGATCCAAGAGAACATAAGAGCTTTCATTGCTGTGAAGAA CTGTCCCTGGATGGGGCTCTTCTTCAAGATCAAGCCCCTTGCTAAGTCTGTAGGAGCTGGAGAGGAGATA GCTGGCCTGAAAGAAGAGTGTGCACAACTACAAAAAGCTTTGGAGAGTTCAGAATCCCAGAGGGAGGAGC TGAAAACAAAGCAAGTCTCCCTTGTCCAAGAAAAGAATGATCTACGTCTTCAGTTGCAGGCTGAACAAGA AACCCTGGCAAATTCCGAAGAGCAGTGTGAATCACTGATTAAATCCAAGGTAGAGCTAGAAGTCAAAATC AAGGAGCTCTCTAGGCAGGTGGAGGAAGAAGAGGAGATCAATTCTGAGCTGACTGCCAGGGGCCGGAAAC TGGAAGATGAATGTTCTGAGTTGAAGAAGGAAATCTACGACCTGGAAGCAATACTGGCGAAGTCAGAGAA GGGGAAGTGTGCAGCGGAGCACAAGGTCAGAAACCTGACAGAGGAGGTGCACTCTCTAAATGAGGAAGTC AGTAAACTTAGTAGAGTAGTGAAGGATGCTCAGGAGACCCAGCAACAAACCCAGGAGCAACTTCACATAG AGGAGGAAAAACTCAGCAACATGAGCAAAGCAAACCTGAAGCTGGCCCAGCAAATTGATGTGTTGGAAGG TGACCTTGAACGGGAGAGGAAAGCCAGAATGAAGTGTGAGAGGGAGAAGCGCAAGCTGCAAGATGAGTTA AAGATGAACCAGGAGGGTGCTGAGAATCTGGAGAGCAGCCGGCAGAAGCTGGCAGAGCAGCTGAGGAAGA AGGAGTTTGAAATGGGTCAGATGAATTCAAAAGTGGAGAATGAGAAGAACCAGGTGTCTCAGCTTCAGAA GATGGTGAAAGAGCTTCAGACTCATATATTAAATTTGAAGGAGGAACTGGAAAGTGAAAGGACCATTAGA GCCAAGGTGGAAAGAGAGAAAGGTGACCTTGTCCAAGACCTAGAAGATTTGAATGAGAGGCTTGAGGAGG CAGGGGGGACCAGTTTGGCTCAGATGGAGATAACTAAGCAACAGGAAGCAAGATTTCAGAAGCTTCACCA TGACATGGAAGAGACCACAAGACACTTTGAGGCAACCTCTGCCTCTTTGAAAAAGAGACACGCAGAGAAC CTGGCTGAGCTTGAGGGCCAGGTAGAACATTTACAGCAGGTCAGGCTGGTGCTAGAACAAGACAAGAGTG ACCTGCAGCTTCAAGTGGATGACCTCCTGAACCGCGTTGATCAGATGGCCAGAGCTAAGGCAAATGCTGA GAAACTCTGTGGTCTGTATGAGAGGCGCTTGAATGAAGCAAATACAAAACTGGATGAGGTGACTCAGTTG GCACATGATCTGACGACACAGAAAACAAAGCTACAGAGTGAGAGTGGTGAGTTCTTTAAAAGACTAGAAG AAAAGGAGGCCCTGATAAGCCAACTTTCCAGGGAAAAGAGCAACTTCACTCGGCAAGTTGAAGAACTGAG AGCACAGCTGGAGGAGGAGAGTAGGTCTCAGAGTGCCCTGAGCCATGCCCTGCAGTCAGCCAAGCATGAC TATGATCTTCTGAGAGAGCAGTATGAAGAAGAACAAGAAGTCAAGGCTGAGCTGCACCGGGCTCTATCTA AAGGCAACAAGGAAACAGTGCAGTGGAGAGCGAAGTATGAACATGATGCTATGCAGAGAACTGAAGACTT GGAGGAAGCCAAGAAGAAGCTGGCGATTCGATTGCAGGAGGCTGCAGAAGCCATGGAGGTGTCCAATGCC AAGAATGCCTCCCTGGAGCGGGCCAGGCACAGGCTCCAGCTGGAGCTGGGGGATGCCCTTTCTGACCTGG GGAAGGCTCGCTCTGTGGCAGCGGCTCTGGGGCAGAAACAGCAGCACTCTGACAAGGCACTGACTTCCTG GAAGCAGAAGCTGGACGAGACCCAAGAGCTGCTGCAGGCTTCTCAGAAGGAGACCCGGGCCCTCAGCTCT GAGGTCCTCACCTTCAGGCAAGCCTGTGAGGAGAGCACCGAGGCACAGGAGACCTTGAAGAGGCAGAACC AGGACCTTCAGGAACAGATTTGTAGTCTCACAAACCAGGTGAGAGAAGGGATCAAGAATTTGGCTGAAGT GGAAAAGGCCAAGAAGTTGATTGAACAAGAGAAGACTGAAGTCCAAGTGAGGCTGGAAGAAACAGAGGGA GCTCTGGAACGGAATGAAAGCAAGATCCTTCGTTTCCAGCTCGAACTCTCAGAGGCGAAGGCAGAGCTGG AAAGGAAGCTGTCGGAGAAAGAGGAAGAAGCAGAGAGGCTGAGGGAGAAGCATCAGCAAGCTATGGGGTC CCTGCAGTCTAATTTGGATTTGGAAGCTTCCAGCAGAATTGAGGCCACCAGACTGAGGAAGAAGATGGAA GGGGACCTCAAGGAAATGGAGATCCAGCTTTGTGCTGCCAACCGGCAGGTGTCACAGATGACCAGAGCCT TGGGCCAGCTTCAGGGTCAAATGAAGGATCTTCACCAACAACTGGATGACAGCATTTACCAGAACAAAGA CCTGAAGGAACAGGTGGCTCTGGCTGAGCAGCGCACCGTTCTTCTTCAGTCTGAACTTGAAGAGCTGAGG ACCTTGCAGGAGCAGACAGAGCGCGGGAGAAAGCTGGCCGAAAAGGAGCTCCTAGAAGCCACAGAAAGAA TCAATCTTTTCCACACCCAGAACACAAGCCTCCTCAGCCAGAAGAAGAAACTGGAGGCCGATGTTGCTCA GGTGCAGAAGGAAGCTGGAGAGATGTTGCAGGCATGTCAGAAGGCAGAAGAAAAGGCCAAAAAGACAGCT GCTGAGGCAGCCAACATGTCAGAAGAGCTGAAGAAAGAACAAGACACTAATGCTCACTTGGAGAGGATGA GGAAGAACATGGAGCAGACAATTAAAGACTTGCAGAAAAGGCTGGATGAAGCAGAACAGACTGCTGTGCT GGGGAGCAAAAAGCAAATCCAGAAACTGGAATCCAGGGTGCGGGACCTGGAAGGTGAACTGGAGAGTGAA GTCAGGCGCAGTGCGGAAGCCCAGCGGGAAGCACGAAGACTGGAGAGAGGCATCAAAGAGCTGACCTATC AGGCAGAGGAAGACAAGAAAAATCTGAGCAGGATGCAGGCTCTGTCTGATAAACTTCAGCTGAAGGTGCA GAGTTACAAACAGCAAGTGGAAGCAGCGGAGGCACAGGCTAATCAATACCTTTCCAAGTATAAGAAGCAG CAACATGAGCTGAATGAAGCCAAGGAGAGGGCAGAGGCCGCAGAATCGCAAGTCAATAAACTCAGAGCTA AAGCAAAGGAACTTGAGAAAAAGGTTCGAGAAGAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001166210
Insert Size	5778 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001166210.1, NP_001159682.1
RefSeq Size	6340 bp
RefSeq ORF	5778 bp
Locus ID	667772

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