Tnrc6c (NM_198022) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	9930033H14Rik; mKIAA1582
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225022 representing NM_198022 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGAAAAGAAAAAGAAAAAGCAGGAAGACAAGAAAAAGAAGGAAGGTGCTCAGAAAAAGGCTGCTG ATCAAAAAACCAAAGTGCCAGAACTTACTAAGACCTGTTCAAGCCAGCCCCAACCTGCCGGTACCAGTCC CAGTACTTCCACCAGCACTATTTCTAGCGGCAGCAATGGCAAACGTGCATCTGCCTGTGGCCAGCAGCCA GCTGCATCCCGTTACCTGCCTCGGGAGGTGCCTCCACGCTTTCGCCAGCAAGAACAGAAGCAGCTGTTAA AGAGAGGCCAGCCATTGCCCACAGGGGGTCTGACCAGTTTGAACTCAGCACAGGCTGCTGGACCTGCAGG GGCCAGCCCTCCTCCCCTCCCTGGAGCTGGGGCACAGCAACATCCCAGCAAGCTCCAACAAGATCTTGGT CACAGTGGATTGGTAGATCATTATGAAAATTCCCACTGGGGACAGCATCCCTCTTACAGAAGTGAAGCCA ACTGCAGCTGGGATAAAGTGATAATAGACAGGACTGAGCCGGAGGTGTGGCCGTCCATCCCAGTAACTGA GACGGAATCTGCCTCAGAATGTCCTACCGACACTGACTCTGCCTCCAACTGTGGCTCAGAGAACAGTAGC ATGGCTACAGGGAGTGCCCAGAGCAGCTTCCCGAGCCACCTGAAGAAGACAAACGGCAGTCATGGCACCA ATGGCGCACTCGTCCAAAGCCCTTCTAACCAGAGTGCCCTTGGGGCAGGAGGAACCAATGGGAACGGGGG CGTGGCCAGAGTGTGGGGTGTAGCCACAAGCTCCAGCTCTGGCCTGGCTCACTGCTCTGTTGGTGGTGGA GATGGGAAAATGGACAACATGATCGGAGATGGGAGAAGTCAGAATTGTTGGGGTGCTTCTAACTCCAATG CGGGCATTAATCTCAACCTTAATCCAAATGCCAACCCAGCTGCCTGGCCTGTACTCGGGCATGAAGGAAC TGTGGCAACAGGCAACCCTTCCAGTATTTGCAGTCCAGTCAGTGCCATAGGTCAGAATATGGGCAGCCAG AATGGGAACCCAGTGGGCGCCCTAGGTGCCTGGGGAAACTTGCTGCCCCAAGAGAGCGCAGAACCACAGA CATCCACTTCTCAGAATGTGTCTTTCAGCGTACAACCTCAGAACCTTAACACTGATGGACCAAATAACAC TAACCCCATGAACTCTTCACCAAACCCTATCAATGCAATGCAGACAAACGGACTGCCAAACTGGGGCATG GCTGTTGGTATGGGGGCCATCATCCCACCCCACCTGCAAGGCCTTCCTGGTGCTAACGGATCATCAGTCT CTCAAGGCAGTGGGAGTGGTGGGGAAGGAATGGGCAGTTCTGTGTGGGGACTGTCCCCTGGTAACCCTGC CACAGGAAGTACCAATTGTGGGTTCAGTCAAGGGAATGGAGACACTGTGAACTCAGCATTAAGTGCTAAG CAGAACGGATCCAGCAGTGCTGTGCAGAAGGAAGGGAATGGAGGGAACGCCTGGGACTCAGGGCCTCCTG CAGGGCCTGGCATCCTTGCCTGGGGAAGGGGCAGTGGCACCAATGGCATTGGTAATATCCATTCAGGAGC TTGGGGCCATCCCAGCCGAAGTTCTTCTAATGGTGTGAATGGGGAGTGGGGGAAGCCCCCAAACCAGCAT TCCAGTAGTGACATCAGTGGGAAAGGATCAACAGGGTGGGACAGTGCCAGTGCTGCCAGCCAGACCCCTG CCCTGCAGCCTGGCAGTGAACACATGAACTCGTGGGCCAAAGCCACATCTTCTGGAACTACAGCAAGTGA AGGAAGCAGTGATGGGTCCGGCAATCACAATGAAGGAAGCACTGGGAGGGAAGGGACAGGAGAGGGCCGG AGGCGAGACAAAGGGGTTCTAGACCAAGGGCACATCCAGTTGCCAAGGAATGACCTTGACCCAAGAGTTC TGTCTAACAGTGGTTGGGGACAGACTCCCGTAAAGCAAAACACTGCCTGGGAATTTGAAGAGTCCCCTAG GTCTGAAAGGAAAAATGACAATGGGACAGAGGCCTGGGGTAGTATAGCTACTCAGCCTTCAAACTCAGGG GGGAAGACTGATGGGTCCATCATGAACAGTACAAATACCTCTTCAGTATCGGGGTGGGTCAGCTCACCAC CTGCCGCCGTGCCAGCAAACACAAGCTGGGGAGACAGCAACAACAAAGCGCCAAGTGGCCCAGGAGTTTG GGGGGACTCAATAAGCTCTACTGCTGTTAACAATGCTGCTGCCACCAAGAGTGGCCATGCTTGGAGTGGG ACTGTAAACCAAGAGGACAAATCACCCACCTGGGGTGAGCCTCAGAAGCCCAAATCCCAAAACTGGGGAG ATGGACAAAGAGCAAACCCAGCCTGGAGCACAGGGGCAGGAGACTGGGCAGATTCATCATCTGTCCTTGG ACATCTAGGGGATGGAAAGAAAAATGGATCTGGATGGGATGCTGATGGTAATAGGTCAGGGTCTGGTTGG AATGATGCCACGAGATGTGGGACCAGTGGCTGGGGCAGTGGCACAAATGCTAAGGTGAATCCAGGTACAA ACTGGGGGGAGTCTCTCAAGCCTGGTCCCCAGCAGAACTGGGCTCATAAGCCCCAAGACAACAATGTGAG TAACTGGGGAGGAGCTGCTTCTGTTAAGCAGACAGGGACAGGGTGGATTGGGGGGCCACTGCCAGTCAAG CAGAAGGACAGCAGTGAGGCGACTGGCTGGGAGGAGCCTTCCCCACCCTCCATCCGACGCAAAATGGAAA TTGATGATGGTACCTCAGCTTGGGGGGACCCAAGCACCTACAACAATAAAACTGTAAACATGTGGGACAG AAACAATCCAGTCATCCAGAGCAGTACCACAGCCCCTGCCACCCCCACGACCCCCACCTCGAGCAGCACC ACACACCGGGCCGAGACGCCGCCTTCACACCAGGCTGGCACTCAGCTGAATCGGTCACCACTGCTCGGGC CAGTTTCATCCGGCTGGGGAGAGATGCCCAGTGTTCACTCCAAGGCCGAGAACTCCTGGGGAGAGCCATC TTCCCCTTGTACCCTGGTGGACAATGGCACAGCAGCGTGGGGGAAGCCTCCCAGCAGTGGCAGTGGGTGG GATCATCCTGCTGAGCCGGTGGTGCCATTTGGAAGAGCCAGCGCTCCTGCTGCCGCCCCAGCCCTGTGCA AACCAGCTTCCAAATCTATGCAAGAAGGCTGGGGCAGCGGTGCGGATGAAATGAACCTGGGCACCAGCCA GTGGGAGGATGAGGACGGGGACATGTGGAACAACGCTGCCTCCCAAGAAAGCAGCTCATCCTGCAGCTCC TGGGGCAACACCTCAAAAAAAGGACTTCAGAAGGGCATGAAGACTCCTGGCAAGCAGGATGAGGCCTGGA TCATGAGCCGGCTGATCAAACAACTCACAGACATGGGCTTCCCGAGAGAGCCAGCCGAAGAGGCCTTGAA AAGCAACAGTATGAACCTTGACCAGGCCATGAGTGCTCTGCTGGAAAAGAAGGTGGACATGGACAAACGT GGACTGGGAATGACCGACTACAATGGAATGGTCACCAAACCCCTCGGCTGCCGCCCACCAATCTCCAAAG AGTCTTCCATGGACCGCCCCACCTTCCTTGACAAGCTCACCCTTTCTTTCTCCAATCAGGATGGCGGCCT CGTGGAAGAGCCCACGACTTCACCGTTTTTGCCTTCCCCAAGCCTGAAGCTCCCCCTTTCCAACAGTGCA CTCCCCAGTCAGGCCCTGGGTGGGGTTGCCTCAGGGCTGGGCATGCAAAACTTGAACTCTTCTAGACAGA TCCCGAGTGGCAATCTGGGTGTGTTTGGCAATAGCGGAGCAGCACAAGCCAGGACCATGCAGCAGCCGCC AGTGCAGCCTCTTAATTCCTCCCAGCCCAGCCTCCGCGCTCAAGTGCCTCAGTTTCTATCCCCTCAGGTT CAAGCACAGCTTTTGCAGTTTGCAGCAAAAAACATTGGTCTCAGCCCTGCACTATTAACCTCGCCAATTA ACCCTCAGCATATGACGATGTTGAACCAGCTCTATCAGCTGCAGCTGGCATACCAACGTTTACAAATCCA GCAGCAGATGTTACAGGCCCAGCGGAATGTTTCCGGACCCATGAGGCAACAGGAGCAGCAAGTTGCGCGC ACAATCACTAATCTGCAGCAGCAGATCCAGCAGCACCAGCGCCAGCTGGCCCAGGCCCTGCTTGTAAAGC AGCCACCACCTCCGCCACCCCCGCCGCACCTGTCTCTGCACCCCTCTGCAGGCAAATCGGGCATGGAGAG CTTCCCCCCTCCCCCCCAGGCTCCCGGCCTTCCCGACCTGCAGACCAAAGAGCAGCAGTCTTCACCCAAC ACCTTTGCTCCTTATCCTCTTGCTGGATTGAACCCGAACATGAATGTCAACAGCATAGACATGAGCAGTG GCTTGTCTGTGAAGGACCCGTCGCAGTCCCAGTCACGCCTGCCTCAGTGGACACATCCCAACTCCATGGG TAACTTGTCTAGTGCTGCTTCTCCCCTGGACCAGAATCCTAGCAAGCATGGTGCTATCCCTGGAGGTCTA AGCATCGGGCCTCCAGGTAAGTCCTCCATTGATGACTCTTACGGCCGGTACGATTTAATTCAGAACAGTG AGTCACCAGCCAGTCCTCCCGTAGCTGTTCCCCATAGCTGGTCACGTGCCAAATCTGACAGTGATAAAAT CTCAAATGGCTCCAGCATCAGCTGGCCCCCAGAATTCCACCCTGGAGTTCCATGGAAAGGACTTCAGAAC ATTGACCCTGAGAATGACCCTGATGTCACGCCTGGCAGTGTCCCTACAGGACCTACCATCAACACCACCA TCCAGGATGTCAACCGCTACCTCCTCAAGAGTGGAGGTAAGCTGTCTGATATTAAATCAACATGGTCCTC AGGCCCAGCCTCTCACACGCAAGCCTCTCTGTCTCATGAGCTGTGGAAGGTACCCAGAAACACTACTGCA CCCACCAGGCCGCCTCCAGGCTTAGCCAATCCCAAGCCTTCCTCCACCTGGGGCACCAGCCCCCTCGGCT GGACCAGCTCCTACTCCTCGGGTTCTGCATGGAGCACTGACACCTCAGGAAGAACAAGCAGCTGGCTCGT TCTGCGCAATCTCACACCCCAGATCGATGGTTCTACACTTCGGACGCTGTGTCTGCAGCATGGACCTCTC ATCACATTCCACCTGAACCTAACCCAGGGCAACGCTGTAGTCCGCTACAGTTCTAAGGAAGAAGCCGCCA AGGCCCAGAAGTCTCTGCACATGTGTGTACTGGGAAACACCACCATCTTGGCTGAGTTTGCCGGGGAAGA AGAAGTGAACCGGTTCCTGGCCCAAGGCCAAGCCCTCCCACCCACCTCCAGCTGGCAGTCCAGCAGCGGG GGCAGCCAGCCCAGACTGGGCACCTCGGGCAGCACCCATGGCCTAGTGCGCAGCGACACTGCCCACTGGA ACACCCCATGCCTGAGTGGCAAAGGCAGCAGTGAGCTGCTGTGGGGTGGGGTCCCCCAGTACTCCAGCAG CCTGTGGGGCCCGCCCAGTGCTGAGGACGCCAGAGTGATCGGCAGCCCCACGCCTCTCAACACTCTGCTG CCTGGGGACCTGCTCAGCGGCGAGTCCATCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_198022
Insert Size	5703 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_198022.2, NP_932139.2
RefSeq Size	8740 bp
RefSeq ORF	5703 bp
Locus ID	217351
UniProt ID	Q3UHC0
Gene Summary	Plays a role in RNA-mediated gene silencing by micro-RNAs (miRNAs). Required for miRNA-dependent translational repression of complementary mRNAs by argonaute family proteins As scaffoldng protein associates with argonaute proteins bound to partially complementary mRNAs and simultaneously can recruit CCR4-NOT and PAN deadenylase complexes (By similarity).[UniProtKB/Swiss-Prot Function]

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