Plxna1 (NM_008881) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2600013D04Rik; mKIAA4053; NOV; PlexA1; Plxn1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225008 representing NM_008881 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCACTGCCACCTCTGAGCTCTCGGACACTGCTGCTGCTGCTCTTGCTGCTGCTGAGGGGTGTGTGGA TAGCCATCAGCTCGCCCCCAGCTGGTTTGGGTCCACAACCTGCCTTCCGCACCTTTGTGGCCAGTGACTG GGGCCTCACTCACCTGGTGGTTCACGAACAGACAGGAGAGGTATATGTTGGCGCTGTGAACCGTATTTAC AAGCTGTCGGGGAACCTAACCCTGCTTCGGGCCCATGTGACAGGCCCTGTAGAAGACAATGAGAAATGCT ACCCACCCCCCAGTGTACAGTCCTGCCCACATGGTCTGGGCAGCACAGACAACGTCAACAAGCTTCTACT GCTGGACTATGCAGCCAACCGCCTGTTGGCCTGTGGCAGTGCCTCACAGGGCATCTGCCAGTTCCTGAGG TTGGACGATCTCTTCAAGCTGGGCGAGCCGCACCACCGCAAGGAACACTACCTGTCCAGTGTTCGAGAGG CTGGCAGCATGGCGGGAGTGCTCATTGCTGGGCCACCCGGCCAGGGCCAGGCCAAGCTCTTCGTGGGCAC ACCCATCGATGGCAAATCAGAATACTTCCCCACTCTGTCCAGTCGCCGGCTCATGGCCAACGAGGAAGAT GCTGACATGTTTGGCTTTGTGTACCAGGATGAGTTTGTGTCATCTCAGCTCAAGATCCCTTCAGACACAT TGTCCAAGTTCCCAGCCTTTGACATCTACTATGTGTACAGTTTCCGCAGCGAGCAGTTCGTATACTACCT CACCCTGCAGCTAGACACGCAGCTTACCTCGCCCGACGCCGCTGGCGAGCACTTCTTCACCTCCAAGATT GTGCGGCTGTGCGTGAATGACCCCAAGTTCTACTCTTACGTGGAGTTCCCCATTGGCTGCGAGCAGGCTG GCGTGGAGTATCGGCTGGTGCAGGACGCCTACCTGAGCCGGCCGGGACAGGCCCTGGCCAAACAGCTGGG CCTGGCTGAGGATGAGGAGGTGTTGTTCACTGTGTTCGCACAGGGCCAGAAGAACCGCGTGAAGCCACCC AAGGAGTCGGCGCTGTGCCTGTTCACACTGAGGGCCATCAAGGAGAAAATCAAGGAGCGCATCCAATCCT GCTACCGTGGAGAAGGAAAGCTCTCCCTGCCTTGGCTGCTCAACAAGGAGCTGGGCTGCATCAACTCGCC CCTGCAGATTGATGACGACTTCTGCGGGCAGGATTTCAACCAGCCACTGGGTGGCACGGTCACCATCGAG GGCACACCCCTTTTTGTCGACAAGGAAGATGGCCTGACTGCTGTGGCTGCCTATGACTACCAAGGCCGCA CCGTGGTTTTTGCTGGCACTCGCAGCGGCCGCATCCGCAAGATTCTGGTGGACCTTGCAAACCCCAGCGG CAGGCCGGCCCTGGCTTATGAAAGTGTGGTGGCTCAGGAGGGCAACCCTATCCTGCGTGACCTGGTCCTC AGCCCCAACCGCCAGTACCTCTATGCTATGACGGAGAAGCAGGTGACGCAGGTGCCTGTGGAAAGCTGTG TCCAGTATACTTCCTGTGAGCTGTGCCTGGGGTCTCGGGACCCCCACTGTGGCTGGTGTGTCCTGCACAG CATCTGCTCTAGGCAGGATGCCTGTGAGCGGGCAGAAGAGCCCCAGCGTTTTGCCTCTGACCTGCTGCAG TGTGTGCAGTTAACTGTCCAGCCGCGCAATGTGTCAGTCACCATGTCCCAGGTGCCGCTCGTGCTGCAGG CTTGGAATGTGCCTGACCTCTCAGCTGGTGTCAACTGCTCCTTTGAGGACTTCACGGAGACTGAGAGCAT CCTGGAAGATGGCCGCATCCATTGCCACTCACCTTCTGCCCGGGAAGTGGCGCCCATCACACAGGGTCAG GGAGACCAGCGGGTGGTGAAGCTCTACCTCAAGTCCAAGGAGACAGGGAAGAAGTTTGCATCTGTGGACT TTGTCTTCTATAACTGCAGTGTTCACCAGTCCTGCCTGGCATGTGTCAACGGCTCCTTCCCCTGCCATTG GTGCAAATACCGCCATGTTTGCACCAACAATGCGGCTGACTGCGCCTTTCTGGAAGGCCGAGTCAACATG TCTGAGGACTGCCCACAGATCCTGCCTTCTACCCACATCTATGTGCCAGTGGGTGTGGTGAAGCCCATCA CCCTGGCTGCACGGAACCTGCCGCAGCCACAGTCGGGTCAGCGAGGGTACGAGTGCCTTTTCCACATCCC AGGCAGCCCGGCCCGAGTCACTGCACTGCGCTTCAACAGCTCCAGCCTGCAGTGCCAGAACTCCTCGTAC TCCTATGAGGGGAATGATGTCAGCGATCTGCCTGTGAACCTGTCGGTTGTGTGGAATGGCAACTTTGTCA TTGACAACCCCCAGAACATCCAGGCCCACCTCTACAAGTGCCCAGCCTTGCGCCAGAGTTGTGGCCTCTG CCTCAAGGCAGACCCACGCTTTGAGTGCGGCTGGTGCGTAGCTGAGCGCCGCTGTTCCCTTCGTCACCAC TGCCCAGCTGACTCACCAGCCTCCTGGATGCATGCCCACCATGGCAGCAGCCGCTGCACAGACCCCAAGA TTCTCAAGCTGTCCCCAGAGACAGGCCCTCGGCAGGGAGGGACTCGACTAACCATCACCGGTGAGAATCT GGGCCTCCGGTTTGAGGATGTGCGGCTAGGCGTGCACGTGGGCAAGGTGCTTTGCAGCCCTGTGGAGAGC GAGTACATCAGCGCAGAACAGATTGTCTGCGAGATTGGAGATGCCAGCACGCTGCGGGCCCATGATGCCC TGGTGGAGGTGTGTGTGCGGGACTGCTCCCTCCACTACCGAGCGCTGTCCCCTAAACGCTTCACTTTTGT GACACCGACCTTCTACCGTGTGAGCCCCTCCCGTGGGCCTCTGTCTGGAGGTACCTGGATTGGCATCGAA GGGAGCCATCTGAATGCTGGCAGTGATGTAGCTGTGTCCATTGGCGGTCGGCCCTGCTCCTTCTCCTGGA GAAACTCACGAGAGATCCGCTGCCTGACACCGCCCGGGCACACTCCTGGCAGCGCCCCTATTGTTATCAA CATCAATCGTGCCCAGCTCTCCAACCCCGAGGTGAAGTACAACTATACTGAAGACCCTACTATCCTGAGG ATCGACCCTGAGTGGAGCATCAATAGTGGTGGGACCCTCCTAACAGTCACGGGCACCAACCTGGCCACTG TCCGTGAACCTCGAATCCGCGCCAAGTATGGAGGCATTGAGAGGGAAAATAGCTGCATGGTGTACAATGA CACCACCATGGTGTGCCGGGCGCCGTCTATAGACAACCCAAAGCGCAGCCCGCCAGAACTGGGAGAGCGG CCAGATGAGATTGGTTTCATCATGGACAATGTGCGCACGCTGCTGGTACTCAACAGCTCCTCCTTCCTCT ACTACCCGGACCCTGTACTGGAGCCACTTAGCCCTACTGGGCTCTTGGAGCTGAAGCCCAGCTCCCCGCT CATCCTCAAGGGGCGGAATCTCCTGCCACCTGCTCCTGGCAATTCCCGGCTCAACTACACGGTGCTCATC GGCTCCACACCTTGTATCCTCACCGTGTCTGAGACACAGCTGCTGTGTGAAGCACCCAACCTCACGGGGC AGCACAAAGTCACGGTGCGGGCCGGGGGCTTCGAGTTCTCACCTGGGATGCTGCAGGTGTATTCGGACAG CCTGCTGACACTGCCGGCCATTGTGGGTATCGGTGGTGGGGGTGGCCTCTTGCTGCTGGTCATCGTGGCT GTGCTCATCGCCTACAAACGCAAATCCCGGGATGCTGACCGTACCCTCAAGCGGCTGCAGCTCCAGATGG ACAACCTGGAGTCACGGGTGGCCCTCGAGTGCAAGGAAGCTTTTGCAGAGCTACAGACGGACATCCATGA GCTGACCAGTGACCTGGATGGTGCTGGCATCCCCTTCCTTGACTACCGCACATATGCCATGCGAGTGCTG TTTCCTGGGATTGAGGACCATCCTGTACTGAAGGAGATGGAGGTGCAGGCCAATGTGGAGAAGTCGCTGA CACTGTTCGGACAGCTGTTGACCAAGAAGCATTTCCTGCTAACCTTCATCCGCACACTAGAGGCCCAGCG TAGCTTCTCCATGCGTGACCGTGGGAACGTGGCCTCACTCATCATGACAGCCCTGCAGGGTGAGATGGAG TATGCCACAGGAGTGCTAAAGCAGTTGCTGTCTGACCTCATCGAGAAAAACCTAGAAAGCAAGAACCACC CCAAGCTCCTGTTACGGAGGACCGAATCAGTGGCAGAGAAGATGTTGACCAACTGGTTCACCTTCCTTTT GTATAAGTTCCTCAAGGAGTGTGCTGGGGAGCCGCTCTTTATGCTGTACTGTGCCATCAAGCAGCAGATG GAGAAGGGCCCTATTGACGCCATCACAGGGGAGGCCCGCTACTCTCTGAGCGAAGACAAGCTCATTCGGC AGCAGATTGACTACAAGACCCTGACCCTGAACTGCGTGAACCCTGAACATGAGAATGCGCCCGAGGTTCC TGTGAAGGGGCTGAACTGTGACACCGTGACCCAGGTCAAGGAGAAGCTCCTGGATGCTGTGTACAAGGGT GTGCCTTACTCACAGCGGCCGAAGGCCGGGGACATGGACCTGGAGTGGCGCCAGGGTCGCATGGCACGCA TCATCCTTCAGGACGAGGACGTCACCACTAAAATTGACAATGACTGGAAGAGGCTGAACACACTAGCTCA CTATCAGGTGACGGATGGGTCATCGGTGGCACTAGTACCCAAGCAGACATCGGCCTACAACATCTCCAAC TCCTCTACCTTCACTAAGTCCCTCAGCAGATACGAGAGCATGCTGCGTACAGCCAGTAGCCCCGACAGCC TACGCTCACGAACACCCATGATCACGCCAGACCTGGAAAGTGGAACAAAACTGTGGCACCTGGTGAAGAA CCACGACCACCTGGACCAACGTGAAGGCGACCGTGGCAGCAAGATGGTCTCTGAGATCTACCTAACCAGG CTGCTGGCCACCAAGGGAACACTACAAAAGTTCGTGGATGACCTGTTCGAGACCATCTTCAGCACGGCGC ACCGTGGCTCAGCCCTACCTCTGGCCATCAAGTACATGTTTGACTTCTTAGATGAGCAGGCCGACAAGCA TCAGATCCACGACTCAGATGTGCGCCATACCTGGAAGAGCAATTGCCTGCCTCTGCGCTTCTGGGTGAAT GTGATTAAGAACCCTCAGTTCGTGTTCGACATCCACAAGAACAGCATAACAGATGCCTGCCTCTCAGTGG TAGCGCAGACCTTCATGGACTCCTGCTCTACATCAGAGCACAAGCTGGGCAAAGACTCACCCTCCAACAA GTTGCTCTATGCCAAGGACATCCCCAACTACAAGAGCTGGGTTGAAAGGTACTATGCTGACATTGCCAAG ATGCCTGCAATCAGTGACCAAGACATGAGCGCCTACCTGGCAGAGCAGTCCCGGCTGCATCTCAGCCAGT TCAACAGCATGAGTGCCCTGCACGAGATCTACTCCTACATCGCCAAGTACAAGGACGAGATTCTGGTGGC TCTGGAGAAAGATGAACAGGCTCGAAGGCAGCGGCTTCGAAGCAAACTGGAGCAGGTGGTGGACACAATG GCCCTGAGCAGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI
ACCN	NM_008881
Insert Size	5685 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_008881.2, NP_032907.1
RefSeq Size	9045 bp
RefSeq ORF	5685 bp
Locus ID	18844
UniProt ID	P70206
Gene Summary	Coreceptor for SEMA3A, SEMA3C, SEMA3F and SEMA6D. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.