Pcsk5 (NM_001190483) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	b2b585C; b2b585Clo; b2b1549C; b2b1549Clo; PC; PC5; PC5/; PC6; SP; SPC6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224998 representing NM_001190483 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTGGGACTGGGGGAACCGCTGCAGCCGCCCGGGACGGCGGGACCTGCTGTGCGTGCTGGCACTGC TCGCCGGCTGTCTGCTCCCGGTATGCCGGACGCGCGTCTACACCAACCACTGGGCAGTGAAGATCGCCGG CGGCTTCGCGGAGGCAGATCGCATAGCCAGCAAGTACGGATTCATCAACGTAGGACAGATCGGTGCACTG AAGGACTACTATCACTTCTACCATAGTAGGACCATTAAAAGGTCTGTTCTCTCGAGCAGAGGAACCCACA GTTTCATTTCAATGGAACCAAAGGTGGAGTGGATCCAACAGCAAGTGGTGAAAAAAAGAACCAAGAGGGA TTATGACCTCAGCCATGCCCAGTCAACCTACTTCAATGATCCCAAGTGGCCAAGTATGTGGTACATGCAC TGCAGTGACAACACCCATCCTTGCCAGTCAGACATGAATATCGAAGGAGCCTGGAAGAGAGGCTACACGG GGAAGAATATCGTGGTGACTATCCTGGATGACGGCATCGAGAGAACCCACCCAGATCTGATGCAAAACTA CGATGCTCTGGCAAGTTGCGATGTGAATGGGAATGACTTGGACCCGATGCCTCGTTATGATGCAAGCAAT GAGAACAAGCATGGGACCCGCTGTGCCGGAGAAGTGGCAGCCACTGCAAACAACTCTCACTGCACTGTCG GGATCGCTTTCAACGCCAAGATTGGAGGTGTGAGAATGCTGGATGGTGATGTCACTGACATGGTGGAGGC AAAGTCTGTCAGCTACAACCCACAGCATGTGCACATCTACAGTGCCAGCTGGGGACCAGATGACGATGGC AAGACTGTGGATGGGCCAGCTCCCCTCACCCGGCAAGCCTTTGAGAACGGCGTGAGAATGGGGCGGAGAG GCCTTGGATCTGTGTTTGTGTGGGCATCTGGCAATGGTGGACGGAGCAAGGATCACTGTTCTTGTGATGG CTATACCAACAGCATCTATACCATCTCCATCAGCAGTACGGCCGAAAGTGGAAAGAAACCTTGGTACTTG GAAGAGTGTTCATCTACACTGGCTACAACCTACAGCAGTGGAGAATCCTATGATAAGAAAATAATCACTA CTGATCTAAGGCAGCGATGCACAGACAATCACACTGGAACATCAGCCTCAGCCCCCATGGCTGCTGGAAT CATTGCCCTGGCCCTAGAAGCCAATCCGTTTCTGACCTGGAGAGACGTGCAGCATGTTATTGTCAGGACT TCCCGTGCGGGACATTTGAACGCTAATGACTGGAAAACCAATGCTGCTGGTTTTAAGGTGAGCCATCTCT ATGGATTTGGACTGATGGATGCCGAAGCCATGGTGATGGAAGCAGAGAAGTGGACAACTGTTCCTCAGCA GCACGTGTGTGTGGAAAGCACAGACCGACAAATCAAGACCATTCGACCAAACAGTGCAGTGCGCTCCATC TACAAAGCCTCAGGCTGCTCGGATAATCCCAACCATCACGTCAATTACCTGGAGCATGTAGTTGTGCGTA TTACCATCACACACCCACGGAGGGGAGACCTGGCCATCTATCTGACATCACCCTCAGGAACCAGATCCCA GCTCTTGGCCAACAGGCTCTTTGATCATTCCATGGAAGGGTTTAAGAACTGGGAGTTCATGACTATTCAT TGCTGGGGAGAACGGGCTGCTGGGGACTGGGTCCTCGAAGTTTATGATACGCCATCTCAGCTGAGGAACT TCAAGACTCCAGGTAAATTGAAAGAATGGTCCTTAGTCCTCTATGGCACGTCCGTACAGCCATACTCCCC AACCAACGAGTTTCCCAAAGTGGAACGCTTCCGCTACAGCCGAGTGGAAGACCCCACAGATGACTACGGT GCTGAAGATTATGCAGGTCCCTGTGACCCTGAATGCAGTGAGGTTGGATGTGACGGGCCAGGACCAGATC ACTGCAGTGACTGCTTACACTACTACTACAAGCTGAAAAATAACACCAGAATCTGTGTCTCCAGCTGCCC TCCTGGCCACTACCATGCTGACAAGAAGAGATGCCGGAAGTGTGCCCCAAACTGCGAGTCCTGCTTTGGC AGCCATGGTAATCAGTGCCTCTCCTGTAAATATGGCTACTTCCTGAATGAAGAAACTAGCAGCTGTGTTA CTCAGTGCCCTGATGGATCATACGAGGATATCAAGAAAAATGTCTGTGGGAAATGCAGTGAGAACTGCAA GGCATGCATTGGATTTCACAACTGCACAGAGTGCAAGGGCGGGTTAAGTCTTCAGGGATCCCGCTGTTCG GTCACCTGCGAGGATGGACAGTTCTTCAATGGTCACGACTGCCAGCCCTGCCATCGCTTCTGTGCTACTT GCTCTGGGGCCGGAGCAGATGGATGTATTAACTGCACCGAGGGGTATGTCATGGAAGAGGGAAGGTGTGT ACAAAGTTGTAGTGTGAGCTACTACTTGGACCACTCTTCAGAGGGTGGCTACAAATCCTGCAAGAGATGT GATAACAGCTGTTTGACATGCAATGGGCCAGGATTCAAGAACTGTTCCAGCTGCCCCAGTGGATATCTTT TAGACTTAGGAACGTGTCAGATGGGAGCCATCTGCAAGGATGGAGAATACATTGATGACCAAGGCCACTG CCAAACCTGTGAAGCCTCATGTGCCAAGTGCTGGGGACCAACTCAGGAAGACTGTATCAGCTGCCCCGTA ACAAGGGTCTTGGATGATGGTCGCTGTGTTATGAACTGTCCTTCCTGGAAGTTCGAATTTAAGAAGCAAT GCCATCCCTGCCACTACACTTGTCAAGGATGCCAAGGTAGTGGCCCTTCCAACTGCACCTCCTGTAGAGC AGACAAGCATGGTCAGGAGCGCTTCCTGTACCACGGGGAATGTCTGGAGAACTGCCCTGTGGGCCATTAT CCTGCCAAGGGACACGCCTGCCTACCCTGCCCAGACAACTGTGAGCTTTGCTACAACCCACACGTCTGCA GTCGATGCATGAGTGGCTATGTCATCATTCCCCCCAACCACACCTGCCAGAAGCTGGAGTGCAGACAAGG TGAATTCCAGGATTCTGAGTATGAAGAATGCATGCCCTGTGAGGAAGGATGTCTGGGATGCACTGAGGAT GATCCAGGAGCCTGTACCTCGTGTGCTACAGGATATTACATGTTTGAGCGGCATTGCTATAAAGCCTGTC CTGAGAAGACCTTCGGTGTGAAATGGGAATGCAGGGCCTGTGGTACTAACTGTGGCAGCTGCGACCAACA TGAGTGCTACTGGTGTGAGGAGGGCTTCTTTCTCTCCGGTGGCAGCTGTGTGCAGGATTGTGGCCCTGGC TTCCATGGAGACCAAGAGTTGGGAGAATGCAAGCCCTGCCACCGAGCCTGTGAGAATTGCACTGGCTCGG GCTACAACCAATGCAGCAGCTGTCAAGAAGGGTTGCAGCTATGGCATGGGACGTGTCTCTGGTCCACCTG GCCTCAGGTGGAAGGCAAGGACTGGAATGAAGCCGTGCCCACTGAAAAGCCATCTTTGGTGAGGAGTCTG CTGCAGGATCGACGAAAGTGGAAAGTTCAAATCAAAAGAGATGCAACGAGCCAGAATCAACCTTGTCACT CTTCTTGTAAAACCTGCAATGGATCTCTCTGCGCTTCATGTCCCACAGGTATGTACCTGTGGCTGCAGGC CTGTGTTCCTTCCTGTCCCCAAGGCACTTGGCCATCAGTCACCAGTGGCAGCTGTGAAAAGTGTTCCGAG GACTGTGTCTCCTGCTCCGGTGCCGACCTTTGCCAACAGTGCCTGAGCCAGCCGGACAACACTCTGCTTC TCCATGAGGGCAGGTGCTACCACAGTTGCCCAGAGGGCTTTTATGCAAAAGATGGTGTTTGTGAACACTG TAGTTCCCCTTGCAAAACATGCGAAGGAAATGCCACCAGCTGTAACTCTTGTGAAGGAGACTTCGTCCTA GACCATGGGGTGTGCTGGAAAACTTGCCCTGAAAAGCACGTGGCCGTGGAAGGAGTCTGCAAGCACTGTC CAGAGAGGTGCCAGGACTGCATCCACGAGAAAACTTGCAAAGAGTGCATGCCTGACTTCTTTCTATACAA TGACATGTGCCATCGTTCCTGTCCCAAGAGCTTCTACCCTGACATGCGCCAGTGTGTCCCCTGCCACAAA AACTGTCTGGAGTGCAATGGCCCCAAGGAAGATGACTGTAAGGTCTGTGCTGATACTTCTAAGGCTCTCC ACAATGGATTGTGCTTGGATGAGTGCCCTGAGGGAACCTACAAAGAAGAAGAGAATGATGAATGCAGAGA TTGCCCCGAGTCCTGCCTGATCTGCTCATCAGCTTGGACCTGCCTGGCCTGTCGGGAAGGCTTCACAGTA GTGCATGATGTCTGCACGGCACCCAAGGAGTGTGCAGCCGTCGAGTACTGGGATGAGGGCTCCCACAGGT GCCAGCCGTGTCACAAGAAATGCTCCCGCTGCTCAGGGCCTTCTGAGGACCAGTGCTATACCTGTCCCAG AGAGACTTTTCTTCTCAATACAACCTGTGTGAAAGAGTGCCCAGAGGGCTACCACACTGACAAGGACAGC CAGCAGTGTGTGCTCTGCCACAGCTCTTGCAGGACCTGCGAAGGACCACACAGCATGCAGTGCCTCTCCT GCCGACCTGGCTGGTTTCAACTGGGCAAGGAGTGCTTGCTGCAATGCAGGGATGGATATTATGGAGAAAG CACCAGCGGTAGGTGTGAGAAGTGTGACAAGAGCTGCAAGTCATGCCGAGGTCCACGGCCCACAGACTGC CAGTCTTGCGATACATTTTTCTTTCTCCTACGCTCCAAGGGACAGTGCCACCGCGCTTGCCCTGAGCACT ACTATGCAGACCAACACGCCCAGACCTGTGAGAGGTGCCACCCCACTTGTGACAAGTGCAGCGGAAAGGA GGCGTGGAGTTGCTTGTCCTGTGTGTGGAGCTACCACCTTCTGAAAGGAATCTGCATCCCAGAATGTATC GTAGGGGAATACAGAGAGGGAAAGGGAGAAAACTTTAACTGTAAAAAATGCCACGAGAGCTGCATGGAAT GCAAGGGTCCAGGCAGCAAGAACTGCACCGGGTGCTCAGCTGGCCTGCTGCTGGACATGGACGACAACCG CTGCCTCCATTGCTGCAATGCCTCCCACTCCCGCAGATCCCAGGACTGCTGCGACTGCCAGAGTTCCACA GATGAGTGCATCCTTCCAGCCAGGGAGGCTGAGTTTTACGAGCACACCAAGACTGCTCTGCTAGTGACCT CTGGCGCCATGCTGTTGCTGCTGCTGGGGGCTGCTGCGGTCGTGTGGCGGAAGTCTCGAAGCAGACCTGT GGCAAAGGGGCGGTACGAAAAGCTGGCAGAACCTACCGTGTCATACTCCTCCTACAGGAGCAGCTATCTT GACGAGGACCAGGTGATTGAGTACAGGGACCGGGACTACGATGAGGACGATGAGGACGACATCGTCTATA TGGGCCAAGATGGCACTGTCTACCGGAAGTTCAAGTATGGGCTGCTGGATGAGACGGAAGATGATGAGTT GGAGTACGATGATGAGAGCTACTCTTACCAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001190483
Insert Size	5634 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001190483.2, NP_001177412.1
RefSeq Size	7193 bp
RefSeq ORF	5634 bp
Locus ID	18552
UniProt ID	Q04592
Gene Summary	This gene encodes a subtilisin-like proprotein convertase that mediates posttranslational endoproteolytic processing of various proprotein substrates traversing the secretory pathway. The encoded protein is an inactive zymogen that undergoes autoproteolytic processing in the endoplasmic reticulum and the Golgi network to generate an active enzyme. Mice lacking the encoded protein die at an early embryonic stage. Conditional inactivation this gene in the epiblast but not in the extraembryonic tissue bypasses embryonic lethality but results in death at birth. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2015] Transcript Variant: This variant (1, also known as PC6B) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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