Col5a1 (NM_015734) Mouse Untagged Clone

CAT#: MC224965

Col5a1 (untagged) - Mouse collagen, type V, alpha 1 (Col5a1), (10ug)



  "NM_015734" in other vectors (2)

CNY 29,450.00


货期*
9周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AI413331
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC224965 representing NM_015734
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGTACACACCCGCTGGAAAGCTGCGCGCCCAGGCGCCCTGCTGCTGTCTTCGCCGCTACTCCTGT
TCCTGCTGCTGCTGTGGGCGCCGCCTTCGAGCCGCGCAGCTCAGCCAGCAGATCTTCTGGAGATGCTAGA
TTTTCACAATTTGCCCTCAGGGGTAACGAAAACCACAGGTTTCTGTGCTACTCGAAGATCTTCCAGCGAG
CCGGATGTTGCCTACCGAGTCTCTAAAGATGCACAGCTCAGCATGCCCACCAAGCAGCTGTACCCTGAGT
CTGGTTTTCCCGAGGACTTCTCCATCCTGACAACCGTGAAAGCCAAGAAAGGCAGCCAGGCCTTCCTAGT
CTCCATTTACAATGAGCAGGGCATCCAGCAGTTGGGGCTGGAGCTGGGCCGCTCCCCTGTCTTCCTCTAT
GAGGACCACACAGGGAAGCCCGGGCCTGAAGAGTATCCGCTTTTCCCTGGCATCAACTTGTCCGATGGCA
AGTGGCACCGAATTGCTCTCAGTGTCTACAAGAAAAATGTCACCTTGATCCTCGACTGTAAGAAGAAGAT
TACGAAGTTCCTCAGCCGCAGTGACCACCCCATAATAGACACCAATGGGATTGTCATGTTTGGCTCCCGG
ATTCTGGATGATGAAATATTTGAGGGTGACATCCAACAGTTGCTTTTCGTCTCTGACAACCGAGCTGCCT
ATGACTACTGTGAGCACTACAGCCCCGACTGTGACACTGCGGTCCCTGACACACCTCAGTCACAGGACCC
TAACCCGGATGAATATTACCCAGAAGGAGAGGGTGAGACCTATTACTATGAGTATCCATATTATGAAGAC
CCTGAAGACCCGGGAAAGGAGCCTGCCCCTACTCAGAAGCCAGTGGAAGCTGCCAGAGAAACCACAGAGG
TTCCTGAGGAGCAGACCCAGCCCCTACCCGAAGCCCCTACAGTGCCTGAGACCAGTGACACGGCTGACAA
GGAGGACAGTCTAGGGATCGGGGACTATGACTACGTGCCCCCAGATGACTATTACACTCCACCCCCATAT
GAAGACTTTGGATATGGCGAGGGTGTGGAGAACCCTGACCAGCCCACCAACCCCGACTCAGGGGCTGAGG
TCCCCACCAGCACCACTGTTACCTCCAACACCTCCAATCCAGCTCCAGGAGAAGGGAAGGATGACCTGGG
CGGCGAATTCACCGAGGAAACCATCAAGAATCTAGAGGAAAACTACTATGACCCGTACTTTGACCCCGAC
TCCGACTCCAGTGTCTCTCCATCAGAGATAGGGCCAGGCATGCCCGCTAACCAGGACACCATCTTTGAGG
GGATTGGAGGACCCCGAGGTGAGAAAGGGCAAAAGGGAGAACCAGCCATCATTGAGCCGGGGATGCTGAT
CGAGGGGCCCCCTGGCCCTGAAGGCCCTGCTGGTCTTCCAGGACCTCCAGGAACTACAGGTCCTACTGGC
CAAATGGGTGACCCTGGAGAAAGGGGTCCCCCTGGGCGCCCAGGTCTTCCTGGAGCTGATGGCTTGCCTG
GCCCCCCAGGTACCATGCTCATGCTGCCGTTCCGGTTTGGAGGCGGTGGCGATGCCGGTTCTAAGGGCCC
CATGGTCTCTGCGCAGGAGTCCCAGGCCCAGGCTATCCTCCAGCAAGCCAGGTTGGCACTGAGGGGACCA
GCTGGCCCAATGGGTCTCACCGGGAGACCTGGCCCCATGGGTCCTCCTGGGAGTGGCGGTTTGAAAGGTG
AGCCAGGAGACATGGGACCTCAGGGTCCTCGAGGTGTGCAAGGCCCACCTGGCCCAACAGGGAAGCCTGG
AAGACGGGGCCGTGCTGGAAGTGATGGAGCCAGAGGCATGCCTGGACAAACAGGCCCCAAGGGTGACCGT
GGCTTTGATGGTCTGGCTGGGTTGCCGGGAGAGAAAGGCCATAGAGGTGACCCTGGTCCTTCTGGCCCGC
CCGGAATCCCAGGAGATGATGGAGAAAGGGGTGACGATGGAGAAGTTGGGCCCAGGGGACTGCCCGGGGA
GCCTGGACCACGTGGTCTGCTTGGGCCAAAAGGCCCCCCAGGGCCTCCTGGACCTCCTGGTGTAACGGGT
ATGGATGGCCAGCCTGGCCCAAAAGGAAATGTGGGTCCCCAGGGAGAGCCTGGGCCGCCAGGACAGCAGG
GTAATCCTGGTGCCCAGGGTCTTCCAGGTCCCCAGGGTGCCATTGGTCCTCCAGGAGAAAAGGGTCCTTT
GGGGAAACCAGGTCTCCCAGGAATGCCAGGCGCTGATGGACCCCCGGGGCACCCTGGAAAAGAAGGTCCT
CCAGGAGAGAAAGGAGGCCAGGGTCCTCCTGGCCCCCAGGGTCCCATTGGCTACCCCGGTCCACGAGGAG
TCAAGGGGGCAGATGGCATCCGAGGTCTGAAGGGCACCAAGGGGGAGAAGGGTGAAGACGGCTTCCCTGG
GTTTAAAGGCGACATGGGAATAAAGGGTGACCGGGGGGAAATCGGCCCACCTGGTCCCCGAGGAGAAGAT
GGTCCTGAAGGTCCAAAGGGTCGAGGTGGTCCCAATGGTGATCCTGGTCCCCTGGGGCCCACTGGGGAAA
AGGGAAAGCTTGGCGTGCCCGGATTACCGGGGTACCCAGGAAGACAAGGGCCAAAGGGTTCCATTGGATT
CCCTGGCTTCCCGGGCGCCAACGGAGAGAAGGGTGGCAGGGGGACACCTGGAAAGCCAGGACCACGGGGA
CAGAGAGGCCCAACGGGCCCGCGGGGTGAACGAGGCCCACGAGGCATCACGGGGAAGCCTGGCCCTAAGG
GCAACTCCGGAGGTGATGGCCCAGCTGGCCCTCCTGGTGAACGGGGACCCAACGGACCCCAAGGTCCCAC
CGGCTTTCCTGGACCCAAGGGTCCCCCGGGCCCACCAGGCAAGGACGGACTCCCTGGACACCCTGGGCAG
AGAGGGGAGACCGGTTTCCAAGGCAAGACTGGCCCTCCAGGGCCCCCAGGAGTGGTTGGCCCTCAGGGTC
CCACAGGAGAGACGGGCCCCATGGGTGAGCGTGGCCATCCTGGTCCTCCAGGCCCTCCTGGTGAACAGGG
CCTCCCAGGTGCTGCTGGGAAAGAAGGAACGAAGGGTGACCCAGGTCCTGCTGGCCTCCCTGGGAAGGAT
GGCCCTCCAGGATTGCGTGGATTCCCTGGGGACCGAGGGCTACCTGGCCCCGTGGGAGCCCTTGGACTCA
AAGGCAGTGAAGGCCCCCCTGGCCCACCAGGTCCTGCGGGTTCTCCAGGGGAGAGAGGACCAGCTGGTGC
CGCTGGGCCCATCGGAATTCCAGGGAGACCTGGGCCTCAGGGACCTCCGGGGCCTGCTGGAGAGAAAGGA
CTTCCTGGCGAGAAAGGTCCACAAGGCCCAGCTGGCCGAGATGGCCTCCAAGGTCCCGTGGGGCTCCCTG
GACCAGCCGGCCCAGTGGGTCCTCCTGGAGAAGATGGAGATAAGGGAGAGATCGGAGAGCCAGGGCAGAA
GGGAAGCAAGGGCGACAAAGGCGAGCAGGGTCCTCCTGGGCCTACCGGTCCTCAAGGCCCGATTGGACAG
CCAGGCCCTTCGGGAGCAGATGGTGAACCTGGCCCTCGTGGACAGCAGGGCCTGTTTGGGCAGAAAGGAG
ATGAAGGTTCAAGAGGTTTCCCAGGACCCCCCGGGCCAGTGGGATTGCAGGGTTTGCCAGGACCTCCAGG
AGAAAAGGGCGAGACAGGAGACGTGGGCCAGATGGGCCCTCCTGGTCCACCAGGCCCCCGAGGACCCTCT
GGAGCTCCAGGTGCCGATGGACCACAGGGTCCTCCTGGAGGGATTGGCAACCCTGGTGCAGTCGGAGAAA
AGGGAGAACCTGGTGAAGCTGGAGATCCTGGCCTTCCAGGAGAAGGAGGTCCCCTGGGACCTAAAGGAGA
AAGAGGGGAGAAGGGAGAGGCTGGCCCCTCTGGTGCTGCTGGACCCCCTGGACCCAAAGGCCCTCCTGGA
GATGATGGCCCCAAAGGCAGCCCTGGCCCTGTGGGCTTTCCTGGAGATCCTGGTCCCCCTGGAGAGCCAG
GCCCCGCAGGTCAAGACGGCCCACCTGGTGACAAAGGGGACGATGGTGAACCTGGGCAGACGGGGTCCCC
GGGCCCTACTGGTGAACCTGGTCCATCTGGGCCTCCAGGAAAGAGGGGTCCCCCAGGCCCTGCAGGCCCT
GAAGGCAGGCAGGGGGAGAAAGGAGCCAAGGGAGAAGCTGGCTTAGAAGGCCCTCCTGGGAAGACTGGCC
CCATTGGCCCCCAAGGGGCCCCTGGGAAGCCTGGCCCCGATGGTCTCCGTGGAATCCCTGGTCCTGTGGG
TGAGCAAGGCCTCCCAGGATCCCCAGGCCCCGATGGTCCACCCGGCCCCATGGGTCCTCCAGGACTCCCT
GGCCTCAAAGGAGACTCCGGTCCCAAAGGTGAAAAGGGCCATCCAGGCCTGATTGGACTCATCGGCCCTC
CGGGAGAGCAAGGTGAAAAGGGTGACCGTGGACTCCCAGGCCCCCAGGGTTCATCTGGTCCTAAAGGAGA
TCAGGGCATCACAGGTCCTTCTGGCCCACTTGGGCCTCCTGGTCCTCCTGGCTTGCCGGGCCCTCCAGGT
CCCAAAGGTGCTAAGGGCTCTTCGGGTCCCACCGGCCCGAAGGGTGAGGCAGGCCACCCAGGACTCCCCG
GCCCACCTGGCCCTCCGGGTGAGGTCATCCAGCCCCTGCCAATCCAGGCCTCCAGGACTCGGCGGAACAT
TGATGCCAGCCAGCTCCTGGACGATGGGGCTGGGGAGAGCTACGTGGATTATGCAGATGGCATGGAAGAG
ATCTTTGGTTCCCTCAACTCCCTGAAGCTGGAGATTGAACAGATGAAGCGACCACTGGGCACCCAGCAGA
ACCCAGCCCGTACCTGCAAGGATCTACAGCTCTGTCATCCTGACTTCCCAGATGGCGAATACTGGGTCGA
TCCCAACCAAGGGTGCTCCAGGGACTCCTTCAAAGTCTACTGCAATTTCACAGCTGGAGGGTCCACGTGC
GTCTTCCCTGACAAGAAGTCTGAGGGAGCCAGAATCACTTCTTGGCCCAAAGAAAACCCAGGTTCCTGGT
TCAGTGAATTCAAGCGTGGGAAACTGCTCTCCTATGTGGATGCTGAAGGCAACCCCGTGGGCGTGGTACA
AATGACCTTCCTGCGGCTGCTGAGCGCCTCTGCCCACCAGAACGTCACCTACAACTGCTACCAGTCCGTG
GCCTGGCAGGATGCCGCCACAGGCAGCTATGATAAGGCTATCCGCTTCTTGGGCTCCAACGATGAGGAAA
TGTCTTATGATAACAACCCCTACATCCGTGCCCTGGTGGATGGCTGTGCTACCAAGAAAGGCTACCAGAA
GACGGTGCTGGAGATCGACACGCCCAAAGTAGAGCAAGTCCCCATTGTGGACATCATGTTCAACGACTTT
GGCGAAGCCTCACAGAAATTTGGATTTGAAGTGGGGCCAGCTTGCTTCCTAGGCTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_015734
Insert Size 5517 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_015734.2, NP_056549.2
RefSeq Size 8420 bp
RefSeq ORF 5517 bp
Locus ID 12831
UniProt ID O88207
Gene Summary Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin (By similarity). Transcriptionally activated by CEBPZ, which recognizes a CCAAT-like motif, CAAAT in the COL5A1 promoter.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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