Col5a1 (NM_015734) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI413331
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224965 representing NM_015734 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGTACACACCCGCTGGAAAGCTGCGCGCCCAGGCGCCCTGCTGCTGTCTTCGCCGCTACTCCTGT TCCTGCTGCTGCTGTGGGCGCCGCCTTCGAGCCGCGCAGCTCAGCCAGCAGATCTTCTGGAGATGCTAGA TTTTCACAATTTGCCCTCAGGGGTAACGAAAACCACAGGTTTCTGTGCTACTCGAAGATCTTCCAGCGAG CCGGATGTTGCCTACCGAGTCTCTAAAGATGCACAGCTCAGCATGCCCACCAAGCAGCTGTACCCTGAGT CTGGTTTTCCCGAGGACTTCTCCATCCTGACAACCGTGAAAGCCAAGAAAGGCAGCCAGGCCTTCCTAGT CTCCATTTACAATGAGCAGGGCATCCAGCAGTTGGGGCTGGAGCTGGGCCGCTCCCCTGTCTTCCTCTAT GAGGACCACACAGGGAAGCCCGGGCCTGAAGAGTATCCGCTTTTCCCTGGCATCAACTTGTCCGATGGCA AGTGGCACCGAATTGCTCTCAGTGTCTACAAGAAAAATGTCACCTTGATCCTCGACTGTAAGAAGAAGAT TACGAAGTTCCTCAGCCGCAGTGACCACCCCATAATAGACACCAATGGGATTGTCATGTTTGGCTCCCGG ATTCTGGATGATGAAATATTTGAGGGTGACATCCAACAGTTGCTTTTCGTCTCTGACAACCGAGCTGCCT ATGACTACTGTGAGCACTACAGCCCCGACTGTGACACTGCGGTCCCTGACACACCTCAGTCACAGGACCC TAACCCGGATGAATATTACCCAGAAGGAGAGGGTGAGACCTATTACTATGAGTATCCATATTATGAAGAC CCTGAAGACCCGGGAAAGGAGCCTGCCCCTACTCAGAAGCCAGTGGAAGCTGCCAGAGAAACCACAGAGG TTCCTGAGGAGCAGACCCAGCCCCTACCCGAAGCCCCTACAGTGCCTGAGACCAGTGACACGGCTGACAA GGAGGACAGTCTAGGGATCGGGGACTATGACTACGTGCCCCCAGATGACTATTACACTCCACCCCCATAT GAAGACTTTGGATATGGCGAGGGTGTGGAGAACCCTGACCAGCCCACCAACCCCGACTCAGGGGCTGAGG TCCCCACCAGCACCACTGTTACCTCCAACACCTCCAATCCAGCTCCAGGAGAAGGGAAGGATGACCTGGG CGGCGAATTCACCGAGGAAACCATCAAGAATCTAGAGGAAAACTACTATGACCCGTACTTTGACCCCGAC TCCGACTCCAGTGTCTCTCCATCAGAGATAGGGCCAGGCATGCCCGCTAACCAGGACACCATCTTTGAGG GGATTGGAGGACCCCGAGGTGAGAAAGGGCAAAAGGGAGAACCAGCCATCATTGAGCCGGGGATGCTGAT CGAGGGGCCCCCTGGCCCTGAAGGCCCTGCTGGTCTTCCAGGACCTCCAGGAACTACAGGTCCTACTGGC CAAATGGGTGACCCTGGAGAAAGGGGTCCCCCTGGGCGCCCAGGTCTTCCTGGAGCTGATGGCTTGCCTG GCCCCCCAGGTACCATGCTCATGCTGCCGTTCCGGTTTGGAGGCGGTGGCGATGCCGGTTCTAAGGGCCC CATGGTCTCTGCGCAGGAGTCCCAGGCCCAGGCTATCCTCCAGCAAGCCAGGTTGGCACTGAGGGGACCA GCTGGCCCAATGGGTCTCACCGGGAGACCTGGCCCCATGGGTCCTCCTGGGAGTGGCGGTTTGAAAGGTG AGCCAGGAGACATGGGACCTCAGGGTCCTCGAGGTGTGCAAGGCCCACCTGGCCCAACAGGGAAGCCTGG AAGACGGGGCCGTGCTGGAAGTGATGGAGCCAGAGGCATGCCTGGACAAACAGGCCCCAAGGGTGACCGT GGCTTTGATGGTCTGGCTGGGTTGCCGGGAGAGAAAGGCCATAGAGGTGACCCTGGTCCTTCTGGCCCGC CCGGAATCCCAGGAGATGATGGAGAAAGGGGTGACGATGGAGAAGTTGGGCCCAGGGGACTGCCCGGGGA GCCTGGACCACGTGGTCTGCTTGGGCCAAAAGGCCCCCCAGGGCCTCCTGGACCTCCTGGTGTAACGGGT ATGGATGGCCAGCCTGGCCCAAAAGGAAATGTGGGTCCCCAGGGAGAGCCTGGGCCGCCAGGACAGCAGG GTAATCCTGGTGCCCAGGGTCTTCCAGGTCCCCAGGGTGCCATTGGTCCTCCAGGAGAAAAGGGTCCTTT GGGGAAACCAGGTCTCCCAGGAATGCCAGGCGCTGATGGACCCCCGGGGCACCCTGGAAAAGAAGGTCCT CCAGGAGAGAAAGGAGGCCAGGGTCCTCCTGGCCCCCAGGGTCCCATTGGCTACCCCGGTCCACGAGGAG TCAAGGGGGCAGATGGCATCCGAGGTCTGAAGGGCACCAAGGGGGAGAAGGGTGAAGACGGCTTCCCTGG GTTTAAAGGCGACATGGGAATAAAGGGTGACCGGGGGGAAATCGGCCCACCTGGTCCCCGAGGAGAAGAT GGTCCTGAAGGTCCAAAGGGTCGAGGTGGTCCCAATGGTGATCCTGGTCCCCTGGGGCCCACTGGGGAAA AGGGAAAGCTTGGCGTGCCCGGATTACCGGGGTACCCAGGAAGACAAGGGCCAAAGGGTTCCATTGGATT CCCTGGCTTCCCGGGCGCCAACGGAGAGAAGGGTGGCAGGGGGACACCTGGAAAGCCAGGACCACGGGGA CAGAGAGGCCCAACGGGCCCGCGGGGTGAACGAGGCCCACGAGGCATCACGGGGAAGCCTGGCCCTAAGG GCAACTCCGGAGGTGATGGCCCAGCTGGCCCTCCTGGTGAACGGGGACCCAACGGACCCCAAGGTCCCAC CGGCTTTCCTGGACCCAAGGGTCCCCCGGGCCCACCAGGCAAGGACGGACTCCCTGGACACCCTGGGCAG AGAGGGGAGACCGGTTTCCAAGGCAAGACTGGCCCTCCAGGGCCCCCAGGAGTGGTTGGCCCTCAGGGTC CCACAGGAGAGACGGGCCCCATGGGTGAGCGTGGCCATCCTGGTCCTCCAGGCCCTCCTGGTGAACAGGG CCTCCCAGGTGCTGCTGGGAAAGAAGGAACGAAGGGTGACCCAGGTCCTGCTGGCCTCCCTGGGAAGGAT GGCCCTCCAGGATTGCGTGGATTCCCTGGGGACCGAGGGCTACCTGGCCCCGTGGGAGCCCTTGGACTCA AAGGCAGTGAAGGCCCCCCTGGCCCACCAGGTCCTGCGGGTTCTCCAGGGGAGAGAGGACCAGCTGGTGC CGCTGGGCCCATCGGAATTCCAGGGAGACCTGGGCCTCAGGGACCTCCGGGGCCTGCTGGAGAGAAAGGA CTTCCTGGCGAGAAAGGTCCACAAGGCCCAGCTGGCCGAGATGGCCTCCAAGGTCCCGTGGGGCTCCCTG GACCAGCCGGCCCAGTGGGTCCTCCTGGAGAAGATGGAGATAAGGGAGAGATCGGAGAGCCAGGGCAGAA GGGAAGCAAGGGCGACAAAGGCGAGCAGGGTCCTCCTGGGCCTACCGGTCCTCAAGGCCCGATTGGACAG CCAGGCCCTTCGGGAGCAGATGGTGAACCTGGCCCTCGTGGACAGCAGGGCCTGTTTGGGCAGAAAGGAG ATGAAGGTTCAAGAGGTTTCCCAGGACCCCCCGGGCCAGTGGGATTGCAGGGTTTGCCAGGACCTCCAGG AGAAAAGGGCGAGACAGGAGACGTGGGCCAGATGGGCCCTCCTGGTCCACCAGGCCCCCGAGGACCCTCT GGAGCTCCAGGTGCCGATGGACCACAGGGTCCTCCTGGAGGGATTGGCAACCCTGGTGCAGTCGGAGAAA AGGGAGAACCTGGTGAAGCTGGAGATCCTGGCCTTCCAGGAGAAGGAGGTCCCCTGGGACCTAAAGGAGA AAGAGGGGAGAAGGGAGAGGCTGGCCCCTCTGGTGCTGCTGGACCCCCTGGACCCAAAGGCCCTCCTGGA GATGATGGCCCCAAAGGCAGCCCTGGCCCTGTGGGCTTTCCTGGAGATCCTGGTCCCCCTGGAGAGCCAG GCCCCGCAGGTCAAGACGGCCCACCTGGTGACAAAGGGGACGATGGTGAACCTGGGCAGACGGGGTCCCC GGGCCCTACTGGTGAACCTGGTCCATCTGGGCCTCCAGGAAAGAGGGGTCCCCCAGGCCCTGCAGGCCCT GAAGGCAGGCAGGGGGAGAAAGGAGCCAAGGGAGAAGCTGGCTTAGAAGGCCCTCCTGGGAAGACTGGCC CCATTGGCCCCCAAGGGGCCCCTGGGAAGCCTGGCCCCGATGGTCTCCGTGGAATCCCTGGTCCTGTGGG TGAGCAAGGCCTCCCAGGATCCCCAGGCCCCGATGGTCCACCCGGCCCCATGGGTCCTCCAGGACTCCCT GGCCTCAAAGGAGACTCCGGTCCCAAAGGTGAAAAGGGCCATCCAGGCCTGATTGGACTCATCGGCCCTC CGGGAGAGCAAGGTGAAAAGGGTGACCGTGGACTCCCAGGCCCCCAGGGTTCATCTGGTCCTAAAGGAGA TCAGGGCATCACAGGTCCTTCTGGCCCACTTGGGCCTCCTGGTCCTCCTGGCTTGCCGGGCCCTCCAGGT CCCAAAGGTGCTAAGGGCTCTTCGGGTCCCACCGGCCCGAAGGGTGAGGCAGGCCACCCAGGACTCCCCG GCCCACCTGGCCCTCCGGGTGAGGTCATCCAGCCCCTGCCAATCCAGGCCTCCAGGACTCGGCGGAACAT TGATGCCAGCCAGCTCCTGGACGATGGGGCTGGGGAGAGCTACGTGGATTATGCAGATGGCATGGAAGAG ATCTTTGGTTCCCTCAACTCCCTGAAGCTGGAGATTGAACAGATGAAGCGACCACTGGGCACCCAGCAGA ACCCAGCCCGTACCTGCAAGGATCTACAGCTCTGTCATCCTGACTTCCCAGATGGCGAATACTGGGTCGA TCCCAACCAAGGGTGCTCCAGGGACTCCTTCAAAGTCTACTGCAATTTCACAGCTGGAGGGTCCACGTGC GTCTTCCCTGACAAGAAGTCTGAGGGAGCCAGAATCACTTCTTGGCCCAAAGAAAACCCAGGTTCCTGGT TCAGTGAATTCAAGCGTGGGAAACTGCTCTCCTATGTGGATGCTGAAGGCAACCCCGTGGGCGTGGTACA AATGACCTTCCTGCGGCTGCTGAGCGCCTCTGCCCACCAGAACGTCACCTACAACTGCTACCAGTCCGTG GCCTGGCAGGATGCCGCCACAGGCAGCTATGATAAGGCTATCCGCTTCTTGGGCTCCAACGATGAGGAAA TGTCTTATGATAACAACCCCTACATCCGTGCCCTGGTGGATGGCTGTGCTACCAAGAAAGGCTACCAGAA GACGGTGCTGGAGATCGACACGCCCAAAGTAGAGCAAGTCCCCATTGTGGACATCATGTTCAACGACTTT GGCGAAGCCTCACAGAAATTTGGATTTGAAGTGGGGCCAGCTTGCTTCCTAGGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_015734
Insert Size	5517 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_015734.2, NP_056549.2
RefSeq Size	8420 bp
RefSeq ORF	5517 bp
Locus ID	12831
UniProt ID	O88207
Gene Summary	Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin (By similarity). Transcriptionally activated by CEBPZ, which recognizes a CCAAT-like motif, CAAAT in the COL5A1 promoter.[UniProtKB/Swiss-Prot Function]

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