Mgam (NM_001171003) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	6030407P20Rik; MGA
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224956 representing NM_001171003 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGACGGAGGTTTAAGAAGTTCAGCAAGCTGGAGCTTGTTCTCATTGTTCTGCTGCTTGTCCTGT TCATCATCGCGGTGGTTCTAATTGTGCTCTTAGCCAACTCGACTCCAGAAAATGCCATAGGTGCTGAAAC AACTGGCACTCCAGACACTACAGTCACCAGGACCACTCTTAACTCTCCCAATTGCCCAGTGCTAAGTGAG CTGGAACGGATAAATTGCATCCCTGACCAGTCATCTAACAAGGGCACATGTGATGAGCGAGGCTGCTGCT GGGATCCTCAGGGATCCATCAGTGTTCCTTGCTATTACTCCAGGAATCATGGCTACAAAATGGAGAGTGA CGTTGTAAACACAAATGCAGGATTCACAGCCACACTGAAAAACTTACCTTCTGCACCCGTGTTTGGAAAC AGCATCGAAAACATCCTGCTTACAGCAGAGTACCAGACATCAAATCGCTTCCACTTTAAGTTGACTGACC AAACCAAGAAGAGGTACGAAGTGCCCCACGAGCACGTGCAACCCTTCAGTGGAAATGCCCCTTCTTCCTT GAACTACAAAGTTGAGGTTTCCAAGGAGCCATTTAGCATCAAAGTCACCAGAAAAAGCAATAACCGTGTG CTGTTTGACTCAAGTATTGGGCCCCTCCTCTTCTCTGACCAGTTCCTGCAGTTCTCTACCCACCTGCCAA GTGCTAATGTATATGGTCTGGGTGAACATGTGCACCAGCAGTACCGACACAATATGAATTGGAAGACCTG GCCCATGTTTTCCAGAGACACAACCCCGAATGAGGATGGAACTAACTTGTATGGTGTGCAGACGTTCTTC CTGTGCCTTGAAGATAACAGTGGATTGTCCTTCGGGGTGTTTCTGATGAACAGCAATGCCATGGAGGTTA CTCTTCAGCCTACCCCAGCAATCACATACCGCACCACTGGGGGAATTCTGGACTTCTATGTGTTCTTGGG GAACACACCTGAGCAAGTTGTTCAAGAATATCTAGAGCTCATTGGACGGCCAGCTCTGCCTTCATACTGG ACACTTGGGTTTCAGCTCAGCCGGTATGATTATAAAAGCCTAGATAATATGAAAGCAGTTGTGGAGAGAA ACCGTGCAGCACAACTCCCTTATGATGTTCAGCATGCTGATATTGATTATATGGATCAGAAGAAGGACTT CACTTATGACCCAGTGAATTTTAAAGGCTTCCCAGAGTTTGTTAAGGAGCTACACAATAATGGGCAGAAA CTTGTTATCATTTTGGATCCAGCAATTTCCAACAACTCTTTCTCAAGTAATCCTTATGGCCCATATGACA GGGGTTCAGCAATGAAAATCTGGGTGAATAGTTCTGATGGCATCAGTCCAGTCATTGGAAAGGTCTGGCC TGGAACAACTGTGTTTCCTGATTACACCAGTCCCAACTGTGCTGTTTGGTGGACAAAAGAATTTGAGCTT TTCCACAAAGAAGTGGAGTTTGATGGAATTTGGATTGATATGAACGAAGTCTCCAACTTTATTGATGGTT CATTTTCTGGATGTTCCCAAAACAACTTGAATTATCCCCCATTCACACCAAAAGTCCTGGATGGGTACTT GTTCTCTAAGACTCTCTGCATGGATGCCGTTCAGCACTGGGGGAAGCAGTATGATGTCCACAATCTATAT GGCTACTCCATGGCCATTGCCACAGCAAAAGCCGTGAAGGACGTGTTCCCAGACAAGAGAAGCTTCATCA TAACCCGCTCTACCTTTGCTGGTTCTGGCAAATTTGCAGCCCACTGGTTAGGAGATAACACCGCCACCTG GAAGGACCTTCAGTGGTCCATCCCAGGCATGCTTGAGTTCAACCTCTTCGGTATCCCAATGGTAGGGGCC GATATATGTGGCTTTGCCCAAGACACATACGAGGAGCTCTGCAGGCGCTGGATGCAGCTGGGGGCATTTT ATCCATTTTCTCGGAATCACAATGGCCAAGGTTACAAGGACCAGGATCCTGCCTCTTTTGGGAATAACTC CCTGCTGTTGAATTCGTCTAGGCACTACCTTAACATCCGCTACACTTTGCTGCCTTACCTCTACACTCTT TTCTACAGAGCACACAGCCGGGGAGACACTGTAGCTAGGCCTCTTCTGCATGAGTTCTATGACGACAACA ATACTTGGGGCATAGACCGGCAATTTCTTTGGGGACCAGGTCTTCTCATCACTCCTGTTCTGGATCAGGG TGCAGAGAAAGTGAAAGCATATGTGCCTAATGCCACCTGGTACGACTATGAAACCGGAGAGGAACTGGGG TGGAGAAAGCAAAGCATTGAGATGCAACTTCCTGGAGACAAGATTGGACTTCACCTTCGAGGGGGCTACA TCTTTCCCACACAACAGCCTGCCACAACCACAGAAGCCAGCCGGAAGAATCCTCTTGGTCTTATTGTCGC CCTGGATGAGAACAAAGAAGCAAGAGGAGAGCTGTTCTGGGATGATGGGGAGTCGAAGGATACTGTGGCC CAAAACATTTACCTTTTCAGTGAATTTTCTGTCACTCAAAACCATTTGGATGTGACAATTTCAAGTCCAA ACTACAAGGATCCCAATAACCTAGAATTCCAGGAAATTAAAATCTTTGGAACACGGGAATTCCGCAATGT TAGAGTGAAACAGAATGGGAATCTCCTTCAAATGTCTCCTCAAGTCACTTACAATCCAAACCTGAAGGTT GCCACCATCACAAATATCCATCTCAGGCTGGGAGAAGCGTACACGGTTGAGTGGGACTTCTTCACAAGGG AGGAGGAAAGGATAGATTGCTATCCAGATGAGCATGGTGCTTCTGAGGCAAACTGCAGTGCCCGTGGCTG CATCTGGGAGGCATCCAACACTACTAGGGGCCCTCCTTGCTACTTTGCTCATGAGCTGTACTCAGTCAGC AATGTTCAGTACGACTCACATGGGGCCACAGCTGACATCTCCTTGAAGGCTTCTACTTATAGCAATGCCT TCCCCTCTACACCTGTGAATAAACTGAAACTGCAAGTGACCTATCACAAGAACGAAATGCTGCAGTTTAA GATCTATGATCCCAACCACAGTCGGTATGAAGTCCCAGTCCCTCTGAACATCCCCAGTGCTCCATTGAGC ACCCCTGAAGGCCGTCTGTATGATGTCCTCATCAAGGAGAATCCATTTGGGATTCAAATTCGCCGGAAGA CTACAGGCACTGTAATCTGGGACTCTCAGCTCCTCGGCTTCACCTTCAATGACATGTTCATCCGCATCTC CACCCGCCTGCCCTCCACATACATCTATGGCTTTGGGGAAACTGAGCACACAACCTTCAAGATAGACATG AACTGGCACACATGGGGCATGTTTTCCAGGGATGAGCCCCCGGGGTACAAGAAGAATTCTTATGGTGTTC ACCCTTACTACATGGGGCTGGAGGAAGATGGCAATGCCCATGGAGTCCTCCTGATGAACAGCAATGCCAT GGATGTGACCTTCCAGCCCATGCCTGCCCTGACATACCGAACCATAGGGGGCATTCTGGACTTCTATGTG TTCTTGGGCCCAACTCCAGAGATTGTTACTCAACAATATACAGAGTTGATTGGTCGGCCTGTGATGGTAC CTTATTGGTCTCTGGGATTCCAGCTGTGTCGCTATGGATATGAGAATGATACTGAAATTGCCAACCTGTA TGATGAAATGGTGGCTAAGCAGATTCCCTATGATGTGCAGTACTCAGACATTGACTACATGGAGAGGCAG CTGGACTTCAAGCTCAGCCCCAAGTTCTCTGGGTTTCCAGCCCTGATCAATCGCATGAAGGCCAATGGGA TGAGGGTCATCCTCATTCTGGATCCAGCCATTTCTGGCAACGAGACAGAGCCCTACCCGGCCTTCACCCG GGGTGTGGAGAATGACGTCTTCATCAGATATCCCAATAATGGAAGCATTGTCTGGGGAAAGGTCTGGCCT GATTACCCTAATATCACTGTGGACCCTTCTTTAGGCTGGGACCATCAAGTGGAGCAATACCGAGCTTATG TGGCCTTCCCAGACTTTTTCCGTAATTCAACTGCTACATGGTGGAAGAAGGAGATTAAAGAACTCCACAG CAACACACAGGATCCTGCCAAGAGCTTGAAGTTTGATGGCCTGTGGATTGATATGAATGAACCTTCCAGC TTTGTGAATGGGGCAGTTCCTTCTGGCTGCAGCGATGCTACTCTGAACCACCCTCCCTACATGCCATATT TGGAAGCCAGGGACAGGGGCCTGAGCAGTAAGACCCTGTGCATGGAGAGTGAGCAGATCCTTCCAGATGG CTCCCGGGTACGGCACTATGATGTACACAACCTGTATGGGTGGTCCCAGACCAGACCTACCTACGAAGCT GTGCAGGAGGTGACAGGAGAGAGAGGGATCGTCATCACACGCTCCACATTCCCCTCTTCTGGACGCTGGG GAGGACACTGGCTGGGAGACAACACAGCTGCCTGGGACCAGCTGGGGAAATCCATCATTGGCATGATGGA TTTCAGCCTCTTCGGCATATCCTATACCGGGTCAGACATCTGTGGGTTCTTTCAAGATGCTGAGTATGAG ATGTGTGTTCGCTGGATGCAGCTGGGCGCCTTTTACCCCTTCTCCAGGAACCACAACACCATCGGGACCC GGAGACAAGACCCTGTATCCTGGAATAAAACCTTTGAGGATATTTCCAGAAGTGTGTTGGAAACCAGATA CACCCTGTTGCCATATCTCTACACCTTGATGTACAAGGCCCACACGGAGGGCAGCACAGTTGTGAGGCCT CTTCTCCATGAGTTTGTGTCAGACCGGGAGACCTGGAATATAGACAAACAGTTCCTGTTGGGCCCAGCCT TCCTAGTCAGCCCTGTGCTTGAGCCTAATGCCAGAAAGGTTGAAGCATATTTTCCCAGGGCCCGCTGGTA TGACTACTACAAGGGTGTAGATATCAATGCAACGGGAGAGTGGAAGACCTTGGAAGCCCCTCTTGAGTAC ATTAATCTTCATATTCGTGGGGGTTATATTCTGCCCTGGCAGGAACCTGCCATGAACACCCATTTAAGCC GGCAGAAGTTCATGGGCCTCAGGGCTGCGTTGAATGCTGAGGGACGTGCTGAGGGCTGGCTCTTCTGGGA TGATGGGAAAAGGATCAATACCGATCAGTACTACTTGGCCAGGTTTTCTGTCAACCAGACTACATTGCTG ACGCATGAAAAGTTTAACAATTACCTCACTGGCACAGCACCCTTATATCTAGGCTACATTGATATCTGGG GATTGAGCAGTTCTTCCATTACCAATGTGAGCATCTCTTGGAACACTAATAATGAAGAAGTCTTTGCCAA CTACAACTCCACAACCAAGATACTGAGTGTCAATATGACTGACAAAAAAATCAGCCTGCACACTTTCAAT TCACTGACATGGAGCTCTTCATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001171003
Insert Size	5484 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001171003.1, NP_001164474.1
RefSeq Size	6488 bp
RefSeq ORF	5484 bp
Locus ID	232714

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