Parp14 (NM_001039530) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1600029O10Rik; ARTD8; BC021340; CoaSt6; mKIAA1268
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224953 representing NM_001039530 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCGTCGGGTTCCTTCCCGCTGCTGGTCGAGGGCTCTTGGGGCCCCGACCCCCCAAAGAACTTGA TCAACAAGTTGCAAGTGTACTTCCAGAGCCGGAAGAAATCCGGAGGAGGGGAGTGCGAGGTCGTCCCGGA GCCGGGGAACCCAGCACGCTTCCGGGTGCTCTTCTCCCCGGAGGATGTTCGGCAGAACGTTCTGGAGAGA GGGAATCATGAGTTAGTGTGGCAAGAAAAAGGAACATTCAAGTTGACTGTCCTGATGCCCACAGACCCTG AGGAAGCCTCAGCCTCTAAGAAGTCAAGGAAGGAGTCTCCAGAAGAGGAGTCCAAGACAAAAGAAGATGC TGTGAAACAAGGTGATTTGGATATAACACATTCTCCCAGCAGTGGATCAGAAAAGACAGAAGATGTCCCA AAAGAATGTGAAAATATTTCCTCCATGGTGGCATTTGAAAACCTGCCTGAGAAAGTATCCGAGATGGTGC TGACCATCCTGGTGGAGAATATAAGCGGCCTCCCCAGTGATGACTTTAAGGTGGAAGTGAACCGAGATTT TGCTGTTGCCGTGGTTACCTTTCAGAAGCCTATAGATATCAAAAAATTTATTGTTGACTGTATCAGCCAC CGCTCAAATCAACAACTCCAGCTTGCCCCGAGACTTCTGGAAACAACGAATGTAGTCAGGGTGGAAAACC TGCCTCCTGGGGTGGACGAGTACCAGCTGCAACTCTTCTTCGAAAACCCTTTCAACGGGGGCGGAAGGGT CGCCCGAGTTGAGTGTTTCCCAGAAGAGAGTTCGGCACTGGTTGAGTTTTGTGACAGCAAAGTGTTAGAC ACCGTCATGGCCAAGACACACAGTTACAACAAGATGCCACTGTCTGTCTTCCCCTATTATCCCTCTCTGG GCACAGCCTTGTATGGTGAGGAGAAGCCTCTGATAAAGCTTCCAGCATCTTTCCAGGAGTCTCTTGATCT TCCTTTGTGGAAGTTCTTTCAGAAAAATAATCATCTGATTGAGGAGATAAATAATGAAATGAGGTGCTGT CACTGTGAGCTGACTTGGTCTGAAATCAATGGTAAACTGACCATCAGACCCGCTGCCACCTTAGTCAATC ATAGACTGAGCATTAAGACCTGGCAGAGAGACGCATCCGCAGTGCTCTCTGGCATCAAGTCTAAATATGG AGTGGAGTTGTTTGAAGTGTGCTCGCCAGTATGGGACATCATAAAGCATGAGTTAGAGTCAGGAGATGAC AGGGTTTTGGTTGAGTTTGAAAAGGAGAGTCTAAACATAGCAGGAAAATCAGAAGACGTCCAAGGCATGA GCCAGAAAATCAGGGAATTAATAGAGAGCACCACTGAAAAGCTCAGAAGGGAAGAGCAGAGTCTGAAGGA GAAAGTGGCCATTTCCCCGGGAAAGCATTATCTTTTGCACCACAGTGGCTTCCTGAAGGATCTAAGCAAG GGGTTCCCAGAGATGGAGATCTCTTACGACGCCACGGCTCAGTTCCTGTACTTGAAGGGCTTCCGTGCAG ATGTGTACAAAGTAAAGTGTGACATCCAAGAGAAGGTGTTCTCCATGGCTCAAAAGGATGTCCAGGTCTC TTCTGAAGTCTTCGAATTTCTGCAGCAAGTAGACAGTCAGAGACTCTCTAAGTCTCTGTTTGAGGCACAG AACATTCTCGCCATATATGAGCTGAAAGGTACAGCTCTTTTCTTAGTTGGAAGTTCTTTCAAAGATCTGG CAGAAGCCGAGACAAAAATGCTCAGTGCTTTAAGTCATAAGCAGATTGAAGTTGAGGACAAGGAGGTTCT GATAAGTAATGGGTGGAAGAAGAAAGTTCACCCTCTGCAAAAGAGACACAGCTCTTGTGCAACCATTATA GTCCAGAATGAATTGACTTCAGAAACCCCGGCAAAGGTCATTGTTACAGGCTGTGTGAAAGAAGTGAATG AAATCCATAGGCAACTTTTTGAGTATTTGGAAAACAACATGAAAGTTGAAAGAGCACTTAAAATCAAACC ATCCTTGATTGTTGACTATTTAAGGACAGACAAGAGGCTATTGTCAAAGATAAAGAAGGCACATGTGTAT GTGCATTTTAAGCCTAAAGACAATCCAAACAGCATTTTGCTAACTGGCTGCAAGAGCAAAGTTCTAGAGT GCATGAATCTAGTCAAGGAAATCCAGGATTCAGTCTGTGTCCAAAGATTCCAGACTGACAAAGCTGGCGT CAGGCACTTCTTCAAAGATAAAGAATCGTATTACAAAACGGAGATTGGTCGTCAGTTCGGATGCGTCATT GAATTGGAGGAGGATAGAGAGGAGAAGGGTGAAGAGGAGGATGGTGAAGAGGAGGAGGGTGAAGAGGAGG GAGAAAGCAGCATTAATGAGCAGAAGTGTCACCTACAGAGGGACATAGCCCCCGGAGTCAAACTGTTCGT CCTCGAGGAAGACCTGAGTCGGTTTCCCGTCGATGTGGTAGTGAATGCAGCAAATGAGAACCTGAAGCAT ATCAGCGGTCTCGCACAGGCTCTCTCAAAGGCCGCTGGCCCTGAACTCCAAACAGAATGTGACCAGATAG TAAAAGAGGGCGGCGTGGTCCTACCCGGCAATGCTGTTATCTCAAAAGCAGGCAAGCTCCCTTGTCACCA CGTCATCCATGCGGTAGGGCCTCGATGGAAGGGAGATAAAGTCCTGGAGTGTGTGAGCCTGCTAAAGAAA GTGGTTAGACAAAGCCTCAGTTTAGCTGAGGAGCACAGGTGCCGCTCCATAGCCATGCCAGCGGTCAGCG CTGGGATCTTTGACTTTCCCTTAGAACTGTGTGTGGCAAACATTGTTTCAGCCATCAAGGAACACTTCCA GCATAAGCGAGACACACACACCTTGAAAAAGATTTACCTGGTGGGCCTACCTGCCAAGGTTGCCAGGGCC TTTGCTGAAGCTGTCAAGACTACATATAAAGACTCCCTTTCCCACACAGCTTTCCCATCCAGTTTAAAGG CTTTAGTACCACTTGGGAAAACGCCCCAGAAACAGGGCAGTCTGCTGGTGTCACCAGAAGGCCTGAGAAT CCGCCTGGTAGAAGAAGGCGTGCAGAATGCTACGACCCATGCCATTGTGAACTCTATTTCCCCGGATCTT AAACTCAACAAAGGACCCCTTTCTCAGGCCTTCTTGGAAAAGGCTGGGCCAAAACTCCAGGAGGAATTGA CCAGGTCAGGACAAGGGGTCTCTGTTGATGTGGGCACAATTCTTCAAACCAGTGGCTGTAATCTCAACAG CCGCCACGTCTTCCATGTGGTGCCTCCACCTTGGAAAAGCAACAACAGTGCATGGTCACTAAAGATCATG AAAAACATCATCCGTGACTGCCTGAAGACCACCGAGAACCTGTCTCTACAATCGATTGCATTTCCAGCAA TAGGAACGGGAAATTTGAGGTTTCCTAAACCTGAATTTGCTAAATTAATAATTTCAGAAGTGCTCAAATT TAGTAGCAGGAACCAACTGAAAACTTTACAAGAGGTTCAGTTTCTGCTGCACCCGAAAGACCATGAAAAC ATTCAGGCATTTTCAGATGAATTTGACAAGAGAAATAATGGAGATCCTAGTGACAAAAATCCCAAGGCTG AAGACACACAAGGTATCTATGGGAGCCTTTCCAGCCCTACTTTAGGAATGCATGAAATGAATATTGGTCC AATCCTCTTCCAAGTGGCCACTGGGAACATCATCAAAGAAGTGGCAGATGTGATTGTCAATTCAACAACA CTCACATTTGATCTCAAATCAGGGGTCTCCAAAGCAATTTTGGAAGGTGCAGGACAAAATGTGGAGCAAG AATGTTCTCTCTTGGCTAAACAGAGTAACCATGGGTATATAGTCACTGGAGGTGGACTATTGCAATGTAA GAACATTATTCATGTTGTTGGTGGGAATGATGTCAAGAAATCGGTTTCCTGTGTTTTGGAAGAATGTGAA CAACGGAATTACTCATCCATTTGCCTACCAGCTATTGGCACAGGAAATGCTCAACAGGACCCAAACGTAG TAGCAAAAGCGATAATAGATGCCATTGAAGAATTTGTCCAGAAAAAATCAGTGCAGGCTGTGAAGAGAGT TAAAGTTGTTATCTTCCAGCCCCATATTCTGCAATTTTTTTACGACAACATGAAAGAAAGAGAAGGCTCT CCAGCTCCCCCCAAGCAGTCGCCTGCCAAGCAGTCAGTGATGTCTAAGATTGCCTCCTTTCTTGGCTTTC CCAAGCAAGCTTCCCCCAAAAAGAACACCCTGGTTTTGGAAAAGAAAATAGAGCACACAGTTTTCCAGGT ATGTGGTTCAGGGGTAGACAGTGTGAACAAAACAATCTCCTGGCTAAAGGAACTGATTACCAAAGAGCAG CTTTCCTACACCAATGACGATGAGTGTGTCAGTGACTTTGACATGGAGGAGTACGAGAAACTGAATGAGA TTCAGAAGGAACTGAACATCACCATTGAGATGAACCAGAAGAAAACTTCGATTCAAGTTTCAGGAATTAG CAGAGACGTGATAAAAGCTAGAGATGAGATTGAGGGCATGATCAAGAGCATTCGATTGGCTAAAGAAAAG GAAAGCCAGGCAGACTACATCTCTACATATGTGGAATGGCAGTATATTGATAAAAACATCACACAGTGTT TTGACAAAATGACCAACATGAAGTTGGAGGTTGCATGGAAGGCCAAGAAGAAGGACACGGTTGTCCAAAT TCACAATCAGGATTTCACGGTGGACTTGAGCACAAACACAGCCACCGCTCCCCAAGGCCAGACTTTCACT GTTCAGCGCCTCGTCAAAGCTGAAGCTGAAATCCCTGCCAATTGGAGTGACATGAAGCAGGACAAATTGC TTCTGGTGAATCTGCAAACCAGTGATCCCGAATACAACATGGTGGCAAGCGCCTTCCGTCAGACCTGCTC AAACTTTTTCATAGAGAAGATTGAAAGGATTCAGAATCCAGCTCTTTGGAGAAGGTATCAGGCATACAAA AAAAGTATGGATGAAAAGAATGGCAATGTAAGAAATGAGAAGCATCTCTTCCATGGGACAGAGGCTAGCT CTCTACCACAGCTCAACAGTAATGGATTTAACCGCAGCTATGCTGGGAAGAACGCTACTGCCTACGGGAA AGGAACCTATTTTGCTGTCAAAGCTTCCTATTCTGCCTGTGACACATATTCCAGACCAGACACCAATGGG AGAAAGTATATGTATTATGTGCGGGTTCTCACTGGAAACTACACGAATGGAAACGCGTCACTGATTGTGC CCCCCTCGAGGGACCCTCAAAATGCTGCTGACTTGTATGACACCGTCACAGATAATGATAAAAATCCAAG TATATTTGTAGTGTTTTATGACAACCAAACGTACCCTGAGTATCTTATCACATTTAGGCAGTAA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001039530
Insert Size	5454 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001039530.3, NP_001034619.2
RefSeq Size	7251 bp
RefSeq ORF	5454 bp
Locus ID	547253
UniProt ID	Q2EMV9
Gene Summary	ADP-ribosyltransferase that mediates mono-ADP-ribosylation of glutamate residues on target proteins (PubMed:27796300). In contrast to PARP1 and PARP2, it is not able to mediate poly-ADP-ribosylation (By similarity). Catalyzes mono-ADP-ribosylating STAT1 at 'Glu-657' and 'Glu-705' and thus decreasing STAT1 phosphorylation, negatively regulates pro-inflammatory cytokines production in macrophages in response to IFNG stimulation (PubMed:27796300). However, the role of ADP-ribosylation in the prevention of STAT1 phosphorylation has been called into question and it has been suggested that the inhibition of phosphorylation may be the result of sumoylation of STAT1 'Lys-703' (PubMed:29858569). Mono-ADP-ribosylates STAT6; enhancing STAT6-dependent transcription (PubMed:27796300). In macrophages, positively regulates MRC1 expression in response to IL4 stimulation by promoting STAT6 phosphorylation (PubMed:27796300). Mono-ADP-ribosylates PARP9 (By similarity).[UniProtKB/Swiss-Prot Function]

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