Cdk5rap2 (NM_145990) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2900018K03Rik; an; mKIAA1633
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224952 representing NM_145990 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTCGGGGATGGAAGAGGAAGGCGCCCTGCCTGGGACCCTCAGCGGTTGCAGTGGCCTTCACCCTG TTCTGCCAAGTGACCTGGATGTCATCAGTGACACTTCTGGCTTGGGAAATGGAGTGCTCCCAAGCATGTC AGAAGAAAAGGTTTCTCCCACCAGAGCCCGGAACATGAAGGACTTTGAAAACCAAATCACTGAGCTGAAG AAGGAGAACTTCAATCTAAAGCTCCGGATCTACTTCCTGGAGGAGAGAATCCAGCAGGAGTTTGCTGGCC CCACCGAGCACATCTACAAGACGAACATTGAACTCAAGGTGGAAGTGGAAAGTCTGAAGCGCGAGCTCCA GGAGAAAGATCAGTTGCTCGTCAAAGCCTCCAAAGCGGTGGAGAGCCTAGCAGAACGGGGTGGTTCTGAA GTCCAGCGGGTGAAAGAAGATGCAAGGAAGAAGGTGCAGCAGGTGGAAGACCTCCTAACGAAAAGGATAC ATCTTCTAGAAGAAGATGTGAAAGCCGCCCAGGCAGAACTGGAGAAGGCCTTTGCGGGGACAGAAACAGA GAAGGCTCTTCGGCTCAGCTTAGAAAGCAAGCTCTCGGCGATGAAGAAGATGCAGGAGGGGGACCTGGAG ATGACTCTGGCTCTGGAAGAGAAGGACAGACTGATTGAGGAGTTGAAGCTGTCTTTGAAGAGCAAAGAAG CTTTAATTCAGTGCCTTAAAGAGGAAAAATCTCAGATGGCATCTCCTGACGAGAACGTGTCATCTGGAGA ACTCCGAGGACTTTCTGCCACTCTGAGGGAAGAAAAAGAGAGAGACGCTGAGGAATGGCAGAAGGAGAGA AACCACTTTGAAGAGAGGATCCAGGCACTTCAGGAGGACCTGAGAGAGAAGGAAAGAGAAATTGCTACAG AGAAGAAAAATAACTTAAAGAGTTATAAAGCCATTCAGGGTCTAACCATGGCATTAAAATCAAAGGAAAG AGAGGTTGAAGAACTTGATTCCAAAATCAAAGAGGTCACTACGGACTCCACCAAAGGCAGAGAGGATCCC TTAAAAACCCAGATCCCCAGATTCCAGCTAAGAGAGGGGTCTGAGGACTGTGAAGCTGCGCTGGTAGAGA AGGAAGCCCTATTGGCCAAGCTACACTCAGAAAATGTCACAAAGAGCACTGAGAACCATAGGTTGCTCAG GAATGTTAAAAAGGTCACCCAGGAGTTGAATGACTTGAAGAAGGAGAAGTTGAGACTAGAGCAGGCCCTG GAGGAAGCCCACCAAGAGGGGAACAGAGGAGCCCGAACCATTCACGACCTTAGAAATGAAGTTGAGAAAC TACGCAAGGAAGTGAGTGAGAGAGAGAAGGCTGTGGAAAAACATTACAAGAGTCTTCCAGGTGAAAGCAA GACAAAGTTTCATACTCAAGAACAAGTGGTCAGAAGTCTGACAGGCAGTGGCAGTCAGGAGGACCTGCTG CTTCAGAAATCCAATGAAAAAGATTTGGAAGCAATACAGCAGAACTGCTATTTAATGACTGCAGAGGAGC TCAAGTTTGGGAGCGATGGCTTAATAACAGAAAAGTGTTCTCAGCAGTCACCAGACAGCAAACTCATCTT CTCCAAGGAAAAGCAACAATCAGAGTATGAAGGGCTGACTGGGGACTTAAAAGCAGAACAGAACATCTAT GCCCATCTGGCAAAGACTCAGGACACAGACAGTGTCAGCAACCTGCAGGCTGAGCTGAAAGAGGTCCTTG CCCTTCGGAAGCAGCTGGAGCAGGATGTTCTTGCCTACCGGAACCTGCAGAAGGCCCTGCAGGAGCAGCT CAGTGAGATTCGGAGTCGAGAAGAAGAGCCGTTTTCATTTTATAGCGATCAAACATCGTATCTGAGTATT TGCCTTGAAGAGCACAATCAGTTCCAATTGGAGCATTTTTCTCAAGAAGAACTCAAGAAAAAGGTCAGTG ACCTCATACAGCTTGTGAAGGACCTGCACACAGACAACCAGCACCTGAAGAAAACCATTTTTGATCTCTC TTCTGTGGGTTTCCAGGGAAGTGACAGACTTGAGTTAACAAAACAAGAAGAGCTTGTGGCTAGCAAGGAG GATGAGGACACACTCAAATTTGAAGCGGACGTTGAGACTCCTTTCCAGAGTGACCAGCATTTGGAGCAAA GTCGTGAGATCATGGAGGACTATGCTGAGGGTGGCTGCAAAAGTGGATATGGAAGGCACATGGATTCCAA TATTTTAGGTCATGATGGGGCCCAAACTCCTGGTGCCTCTGAAGAGCACACCCTAGAAGATGAGCTCCTG GGCCTGCTTGCCACTCTCTTCAGTAAGAAGGCCACACCGTTGCTGGAATCCAGGCCAGATCTTCTGAAAG CACTCGGTGCCCTACTTTTGGAACGCATATGCTTGGCAGAGCAGGGTCGTCCTGGAGATCACCTTGATAG TAAAACTGAGAAGGCACTTCAGCAAGTAGCTGTTCAGTTGAGAGATGAACTTGGCCATTCCTTCCCTGCC AACTCGTTCTCCAAGTCATACAATGAAGTCAAGTCAATGTGGGGAAATTGGCTGGTAAAGACAGGAGACG AAGACACGGTGGAGCTGAAGAGTGTTTCAGTGCAGACCATGGCCATAGAGGACACCCCGCATGGATTCAA ACCTCAAAGCAAGAGAGATGCTTGGGCAGAGAAACAAGAAGAGGCCATTTTCAGCACTGAGCTTGAGTCA GAGGCGTTGGGTGAGATGCCAGGGCAACAGGCCACTCACCTCTCATTCCCCAGTGCCATCAACCCGGATG CCGAGAAGACGGGCTTGTTGATCCAATTAAAGACACCTGAGTTATTGGAAAATTTATATAATCTTCCTGC TTCGCCAGAGGTGGTGGTGGCTCAACTCCAAGGCCAGGTTCTAGAGCTGCAGAGGGAGCTGAAAGAATTC AAAACCCGCAACAAGCAACTCCATGAGAAGCTAATTCTGGCAGAGGCAATGATGGAGGGGCTGCCGGTGC CCAACTCTGCGCTGGTGAATGTGCCTGCAGCCCAGGCTGTTGTGAGAACAGCCTTCCAGGACAACCCAGG CGAGCAAGAAGGACCTGAAACCACACAGTCTGCTGGGAGAGACAAGGACATGGACAGTGACCAATATACA AGCTTTGAGATCGATTCTGAAATCTGTCCACCTGATGACCTTGCCTTATTACCAGCATGCAAAGAGAATC TTGAAGACCTCCTAGGCCCATCCTCCATAGCCACTTACCTGGATTCCAAGAGTCAGCTCTCTGTTAAAGT CAGCGTGAATGGAACTGACCAGTCTGAGAATATCAATATCCCTGATGACACAGAAGACTTAAAACAGAAA ATCCACGACTTGCAAACCGAGCTGGAAGGCTACCGGAACATCATTGTCCAGCTACTGAAGCACTCTCAGT GCAGTGAGGCTATTATCACAGTTTTGTGTGGGACAGAAGGGGCCCAAGATGGCTTGAACAAGCCTAAGGG CCATATTGATGAGGAAGAGATGACATTTTCAAGTTTGCACCAAGTACGATATGTGAAGCATATGAAAATC CTCCGTCCACTAACCCCGGAGATGATTGATGGCAAGATGCTAGAGAGCCTCAAGCAGCAGCTGGTGGACC AGGAGCAGGAGCTACAGAAGGAGCAGGACTTGAACTTGGAACTTTTCGGTGAAATCCATGACCTGCAGAA CAAGTTCCAAGACCTCTCGCCATCAAGATACGATTCATTGGTTCAGTCCCAAGCGAGGGAGCTCTCCCTT CAGCGACAGCAAATTAAGGATAGCCATGGCATCTGTGTCATTTACCGTCAACACATGAGCACCATGATCA AGGCGTTTGAGGAGCTTCTGCAGGCCAGTGATGTGGACAGTTGCGTGGCCGAGGGCTTCCGGGAGCAGCT GACACAGTGTGCTGGACTGCTAGAGCAACTGGAGAGGCTGTTTCTCCATGGAAAATCAGCCAGAGTGGAA CCACATCCCCAGAATGAACTGCTCAAGGGGTTAAGGACTGTGGAAGGCAACTTACCCTACCACCATCTCC TGCCTGAATCTCCTGAGCCTTCTGCCTCCCATGCTCTGTCAGATGATGAAATGTCTGAGAAGTCCTTCCT GTCTAGAGACCCGAAGCCAGACAGCGATACTGAGAAGTACCCAGCAATGGCGAGCCATTTTCCTCAAGAC TTACTAATGGAACACATACAGGAAATTCGAACTTTGAGAAAGCATTTGGAAGAATCTATTAAAACAAATG AGAAGCTCCGGAAACAGCTGGAACGGCAGGGGTCTGAGACTGACCAAGGCTCCAGAAATGTTTCTGCTTG TGGCTTAGCACTGCACAGCTCTCTGACGTCAGAGATACATTTCCTGAGGAAGCAGAACGAGGCCCTCAGT ATGATGCTCGAGAAGGGGTCCAAAGATAAACAGAAAGAGAGTGAAAAATTACGTGAGTCCCTGGCTAGGA AAGCTGAGAGTCTTGAGCAACTTCAGCTAGAGTACACCAGTGTAAGGGAAGAAAATGAAAGGTTACAGAG AGACATCATTGAGAAGGAGAGACACAACCAGGAGCTGACTGAGGAGGTCTGCAGCAGCCGCCAGGAACTG AGCAGGGTGCAGGAAGAGGCGAAGTCAAGACAGCAGCTGCTCTCACAGAAGGACAAGCTGCTTCAGTCCC TCCAAATGGAGCTCAAGGTGTATGAGAAACTGGCCGAGGAACATCCGAGGCTACAGCAAGATGGCAGCAA ATGTCCTGAGGCCAGCGACAATTCATTTGATCTATTTGAATCCACCCAGGCAATGGCACCTAAATCAGCT TCTGAGACTCCTCTGCTCTCAGGAACTGATGTTGACTCGCTCTCCTGTGACAGTACCAGTTCTGCCACCA GCCCTACTAGCATGCCCTGCCTGGTTGCTGGCCACCACATGTGGGCCAGCAAGAGTGGCCACCATATGCT GGGCCTGATTGAGGACTATGATGCCCTCTATAAGCAGATCAGCTGGGGCCAGACACTGCTTGCCAAGATG GATGTTCAAACCCAAGAGGCTCTGAGCCCCACAAGTCACAAGCTGGGACCAAAGGGTTCTTCATCCGTGC CTCTGAGCAAGTTTCTTTCCAGTATGAACACAGCCAAGCTGGTCCTGGAAAAAGCATCCAGGTTGCTGAA GCTCTTCTGGAGGGTCTCAGTCCCCACCAACGGCCAGTGTTCCCTTCACTGTGAGCAGATTGGAGAAATG AAGGCAGAGAACACGAAACTACACAAAAAATTGTTTGAACAAGAAAAGAAGCTGCAGAACACAGCAAAAC TATTGCAGCAGAGCAAGCACCAGGAGAAAGTCATCTTTGATCAGTTGGTCATCACCCACCAAGTCCTTCG GAAGGCCAGGGGAAACCTGGAGCTCAGGCCTGGGGCCACCCGTCCAGGAGCATCCAGTCCCAGCAGACCG GGCTCATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_145990
Insert Size	5469 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_145990.4, NP_666102.2
RefSeq Size	11840 bp
RefSeq ORF	5469 bp
Locus ID	214444
UniProt ID	Q8K389
Gene Summary	Potential regulator of CDK5 activity via its interaction with CDK5R1. Negative regulator of centriole disengagement (licensing) which maintains centriole engagement and cohesion (PubMed:20627074). Involved in regulation of mitotic spindle orientation (PubMed:20460369). Plays a role in the spindle checkpoint activation by acting as a transcriptional regulator of both BUBR1 and MAD2 promoter. Required for the recruitment of AKAP9 to centrosomes (By similarity). Plays a role in neurogenesis (PubMed:20471352). Contrary to higher mammalian orthologs, including human, chimpanzee, bovine and dog, does not interact with EB1/MAPRE1, therefore its function in the regulation of microtubule dynamics is unclear (PubMed:19553473).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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