Pappa2 (NM_001085376) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	PAPP-A2; Pappe; PLAC3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224932 representing NM_001085376 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGTGTTGGAAGGTCCTGAGAATAAGCCTGGTGGTCCTGACTGGGTGGGCACTCAGTACCACCAACT CAGAGCAGGGCTGGACACGCAAGAAATTCCTGGCCCAGAAGGAACACCTGAACCAAGTGCTGTTGGAAGG GGAACGCTGTTGGCTGGGGTATAAGGTTCGAAGACCCAGGACTGCTCCACAGCACCATCTCTTTGGGGTC TACCCAAGCCGCCTCGAAAACTACCCAAGGTCCTATCCTGTGGGGACACAGCAGCAAACCCTGTATGCAG AATGGAGTCAGCGAAGCCCAGAGAGACAGGCTCCGAGTGGTATTCCCCGAGACCTGACTGAAAACCCAGC AGGAGCAAGGAGAGAGTCTCAGCAGCCAGCTGCCCCGTGGGTAGGGGATGGTCCTATTGGGCAATCGCAG CTGCTGAGAAATGATGACACTTATCTTGGCGAGGGAGGATCTAAGGAGGCTCTAGGTGAGCCTGCGATTC AGGGAAGCTCAGAAATTGCTGTCTCCACCATTGCTACCTTCGCATATCAGAAGGGACCCACAGCAGAGAC CCAGAGGAAGGGCCAGTCCAAGTCTAGGCTTCCTCGCCAAGTGCAGAAGAGGCAGGCAGAAGACATGACT GGAGACCCCCAGAATACCCCTCAAGGTTTCCAGCTTTGGCCTAAAGAGCCACTTAAGCATGGAGATAGTG ACAGTCCATTGGAGGGCACCATCCAAAATGGTGGAGGGGCCTCTCTCCGGAGAGTAGAGACCTTTAACTC CCAAGGAGGATTGCCTGTACTGTATTTCACTGGGAAGAGGGAGCGGCTGTTGCTGCGTCCAGAAGTGCTG GCTGAGATTCCCCGGGAGGCGTTCACAGTGGAAGCCTGGGTGAGACCAGAGGGAGGACAGAGCAACCCAG CCATCATTGCAGGTGTGTTTGATAACTGCTCCCACACAGTCAATGACAAGGGCTGGGCCCTGGGGATCCG CTCAGGGAAGGACAAGGGGTGGAGAGATGCTCGCTTCTTCTTTTCCCTCCGCACTGACCGGATGAAGAAA GCCACTATTGTGACTGGCCACAGCCGTTACCAGCCAGGCATGTGGACTCATGTAGCAGCCACTTACGATG GACAGCATACAGCTCTGTATGTGGATGGCACTCGAGTGGCTAGTAGTCGTGACCAGTTTGGACCGCTAAA TAGCCCTTTCATGGCGTCTTGCCGCTCTTTAATCCTGGGAGGGGACAGCTCCGAGGATGGACATTATTTC CGTGGTTACCTGGGCACACTGGTAATCTGGTCGACCGCCCTTTCACAAACCCACCTCCAGCACAGTCCCC TGCATCCAAGTGCAGCAGATGAGTTGACCACCCTGATCCTGACAGCCACTTTCGATCCTCTGATGGAACA GTGGGCTCCATTTAGAGATGACTTATATCCCCGGCTGGAGGTTCTCCAGGACTCTGAGTCACAGCCTGAG ATTCTGTCACCTTTACAACCCCCACCTTGTGGGCAAACAGCTTGTGACAATGTGGAACTCATCTCCCAGT ACAATAAGCATGGGCCCCTTCGTAGAGAGAAAGTGATCCGCTACCAGGTGGTAAACATCTATGATGATGA GGGTCTGCATCCCATTGTGAGTGATCATCAGATCCGCCGTCAGCATGAGGCCCTCAACCAGGCCTTCAGC CGCTACAATATCAGCTGGCAGCTGAGTGTCCACCGAGTCCACAACTCCACCCTGCGCCACCGGGTTGTGC TTGTTAATTGTGAGCCCAGCAAGATTGGCAATGACCACTGTGACCCTGAATGTGAGCATCCACTCACCGG CTTCGATGGGGGCGATTGCCGCCTGCAGGGACGCTGCTACTCCTGGAATCGCAGGGATGGCCTCTGTCAC GTGGAGTGCAACAACATGCTGAATGACTTCGATGATGGAGACTGCTGCGATCCGGAAGTGACTGATGTCC GAAAGACTTGCTTTGATCCTGACTCACCCAAGAGGGCATACATGAGTGTGAAGGAACTAAAGGAGGCCTT GCAGCTCAACAGCACTCACTTCCTTAATGTCTACTTCGCCAGCTCAGTCCGGGAAGACCTTGCAGGTTCT GCCACATGGCCCTGGGACAAGGAAGCTCTCAGTCACCTGGGTGGTGTTGTCCTCAACCCAACCTATTATG GCATGCTTGGCCACACCAACATCATGATCCATGAGGTGGGACATGTCCTGGGACTCTACCATGTATTCAA AGGAGTCAGTGAAAGAGAATCCTGTGATGATCCCTGTAGAGAGACAGTGCCATCCATGGAGACAGGAGAC CTGTGTGCTGACACTGCTCCCACACCCAAGAGCAAGCTGTGTCGGGACCCAGAGCCAGCTAATGACACCT GTGGCTTCACCCTCTTCCCAGGGGCTCCGTTCAATAACTACATGAGCTACACAGATGATGAGTGCACTGA CAACTTCACCCCTAACCAAGTGGCCCGGATGCATTGCTACTTGGACCTCGTATACCAGCAATGGAGTGAA AGCCAAAAACCTACCCCCATACCCATACCACCCATGGTTATTGGGCAAACCCACAAGTCCCTTACTATCC ATTGGCTGCCTCCAATCAGTGGAGTTGTGTATGATAGGGCCCCTGACAGCATGTGTAGTGCCTGCACTGA AGATGGGACATTCCGTCAGTATGTATACAAGGCATCCTCTGGGCGGGTGTGTGACTCTTCAGGTTACTGG ACTCCAGAGGAGGCTGTGGGGCCTCCTGATGTCGATCAACCCTGTGAGCCTAGCTTGCAGGCCTGGAGTC CTGAGCTCCACCTAAACCACATGAATATGACCGTGCCCTGCCCAGCAGAAGGCTGTAGCTTGGAGTTGCT TTTTCAATACCCGGTCCAGGCGGATACCCTCACCCTCTGGGTCACTTACCTCTCCATGAACTCTTCCAAG GCATTCTTTGACATAGAGATCTTGCTAGAACACAAAAAATCTATGCACCTTGGACCTTTAAACACTTTTT GTGACATACCGCTCACCATCAAACTCCACATGGATGAGAAGGTCATGGGAGCGAAAGTCTACACCTTTGA TGAACGAATGGAAATTGATGCTGCTCTCCTGACCTCTCGGCCCAATAGTTCCTTGTGTTCTGGATGCAAG CCTATGAGTTACCAGGTTCTTCGAGAGCCTCCTTTTTCCAGTGGCTTGCCCATGGTGGTGACACATCCAC ACAGAAAGTTCACAGATACGGAGGTCATCCCTGGGCAGATGTATCAGTACCAAGTTCAAGCTGAAGCTGT AGGGGAACTCGGAGAAGCTTCACCTCCTCTGAGCCACATTCACGGAGGCCCTTACTGTGGAGATGGGAAG GTAGAAAGGAGTATCGGAGAGATGTGTGATGATGGAGACCTGTTGAATGGAGATGGGTGCTCAAGAGCAT GTGAACTGGAGGAAGGCTTCAATTGTGCAGGGGAGCCAAGCCTTTGCTACAGGTATGAGGGAGATGGGGT TTGTGAAGATTTTGAGAAGGAAAGCATCATCACAGACTGTGGCCTCCACACTCCTGAAGGATATTTGGAT CAGTGGGCTAGCCAGGCTTATTCCTACCATGAAGACAAGGAGAAATGTCCTGTTTCCCTGGTAACAGGAG AACCTCATTCAATGATCTGTACTTCATGGCACCCGGATTCATCCCCATATCATTCCTTTCCTGGCTGGTT TCCCTGTGTCTTCAGTCTGAAGAAAGACCAGGATGCAGGCAGTGAGCAGGCTAAAGATAGCCTGCAGAAG GACAATGAAATTTGGCTTGAAGTGTGTTTCAATAGGCCAGGGGTTGCAGTGGCAATTTACATTTTCTTGG CATCGGATGGCGTGACCCCTGGGGAGCATCAGCAGGCAACAGTGACCCTTTACCTGATTGATGTCAGTGG AAGCAACCACTCTCTTGGAACCTATGGACTGTCGTGTCAACAAAATCCACTGGTTATCAATGTGAGCCAT CATGTGAATGTCTACCCCCAACATACCTCCTCCATGCTGCTGAATTTTTCATCCCCACTGGTGGGCATCT CAGCAGTGGCTCTAAGGACATCCTCCCAGACCAGTTCTTCAGCTCCCAGTAACTGCATCCCAGAGCAAAA TTATGAGGGAAAGAGCTGTGCTCAACGACCTTGTGGAGAACAGGGAAGATGTGCGCCATTGCTACTCGAT CATACGGACATGGTGAATTGTACCTCCAGTAGCCCAGGTCACATGGAGTGCGCTATCACTTGTCAAAGGG GGTATGTCCTTCAGACCAGCAGTGGGCAGTACCTGAGGAACATGCAGAAAGAGATTCTCCTTACTTGTTC CTCTGGCCACTGGGACAAGGATGTGATCTGCAAGCGTCTTGACTGTGGTGTCCCAGACTCCTCCTTGGTG AATTATGCAAACTTCTCCTGCTTGGAGGGTACAGACTTTCTGAAACAATGTTCCATCTCGTGTGTCCCAC CGGCTAAGCTCCAAGGACTGAACCCATGGCTGACATGTCTTGAAGATGGACTCTGGTCTCTCCCTGAAGT CTACTGCAAGTTGGAATGTGAAGCTCCTCCAGTTATTCCCAATGCCAATCTGCTCCTGCCACATTTCCTG GAAGGCAACCATGATGTGGGCACCATCTGCAAATATGAGTGCAAACCAGGCTACTATGTGAAGGAGACTT CAGGAAGTCAAGGCAAGAACAAGTTCCTGAAGATCCAGTGCCTGGATGATGGAAGCTGGGAACAAGGGAG CTGCGTCCCCGTGGTTTGTGAGCCTCCTCCTCCGGTGTTTGAAGGCATGTACGAATGTACTGATGGCTTC AAGCTGGACAGCCAGTGTGTGCTCAACTGTAACCAGGAAACGGAAGGGATTCCCATCCTCTGCACTAAAG AGGGGTTGTGGACCCAGGAATTCAAGTTGTGTGAAAACCTGCAAGGAGAATGCCCACCACCCACCTCAGA GCTGAATTCTGTGGAGTACAAGTGTGGACAGGGATATGGGATCGGTGCAGTGTGCTCCCCATCATGTGTA ATCCCCCCTAGTGACCCTGTGATACTACCAGAGAATGTGACTACTGACACTTTGGAGCACTGGATGGAAC CTGTCAAAGTCCAGAGTATCGTGTGTACCGGCAGGCGCCAGTGGCACCCAGATCCCTCTCTGGTGCACTG CATCCAGTCATGTGAGCCTTTCCAAGCAGATGGTTGGTGTGACACTATTAATAACCGGGCCTACTGCAAC TATGACGGGGGAGACTGCTGCTCTTCCACACTCTCTTCCAAGAAGGTCATTCCATTTGCTGCTGATTGTG ACTTGGATGAATGCACCTGTCGAGACCCCAAGGCAGAAGAAAACCAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001085376
Insert Size	5370 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001085376.2, NP_001078845.1
RefSeq Size	8602 bp
RefSeq ORF	5370 bp
Locus ID	23850

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