Pappa2 (NM_001085376) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	PAPP-A2; Pappe; PLAC3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR226285 representing NM_001085376 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGTGTTGGAAGGTCCTGAGAATAAGCCTGGTGGTCCTGACTGGGTGGGCACTCAGTACCACCAACT CAGAGCAGGGCTGGACACGCAAGAAATTCCTGGCCCAGAAGGAACACCTGAACCAAGTGCTGTTGGAAGG GGAACGCTGTTGGCTGGGGTATAAGGTTCGAAGACCCAGGACTGCTCCACAGCACCATCTCTTTGGGGTC TACCCAAGCCGCCTCGAAAACTACCCAAGGTCCTATCCTGTGGGGACACAGCAGCAAACCCTGTATGCAG AATGGAGTCAGCGAAGCCCAGAGAGACAGGCTCCGAGTGGTATTCCCCGAGACCTGACTGAAAACCCAGC AGGAGCAAGGAGAGAGTCTCAGCAGCCAGCTGCCCCGTGGGTAGGGGATGGTCCTATTGGGCAATCGCAG CTGCTGAGAAATGATGACACTTATCTTGGCGAGGGAGGATCTAAGGAGGCTCTAGGTGAGCCTGCGATTC AGGGAAGCTCAGAAATTGCTGTCTCCACCATTGCTACCTTCGCATATCAGAAGGGACCCACAGCAGAGAC CCAGAGGAAGGGCCAGTCCAAGTCTAGGCTTCCTCGCCAAGTGCAGAAGAGGCAGGCAGAAGACATGACT GGAGACCCCCAGAATACCCCTCAAGGTTTCCAGCTTTGGCCTAAAGAGCCACTTAAGCATGGAGATAGTG ACAGTCCATTGGAGGGCACCATCCAAAATGGTGGAGGGGCCTCTCTCCGGAGAGTAGAGACCTTTAACTC CCAAGGAGGATTGCCTGTACTGTATTTCACTGGGAAGAGGGAGCGGCTGTTGCTGCGTCCAGAAGTGCTG GCTGAGATTCCCCGGGAGGCGTTCACAGTGGAAGCCTGGGTGAGACCAGAGGGAGGACAGAGCAACCCAG CCATCATTGCAGGTGTGTTTGATAACTGCTCCCACACAGTCAATGACAAGGGCTGGGCCCTGGGGATCCG CTCAGGGAAGGACAAGGGGTGGAGAGATGCTCGCTTCTTCTTTTCCCTCCGCACTGACCGGATGAAGAAA GCCACTATTGTGACTGGCCACAGCCGTTACCAGCCAGGCATGTGGACTCATGTAGCAGCCACTTACGATG GACAGCATACAGCTCTGTATGTGGATGGCACTCGAGTGGCTAGTAGTCGTGACCAGTTTGGACCGCTAAA TAGCCCTTTCATGGCGTCTTGCCGCTCTTTAATCCTGGGAGGGGACAGCTCCGAGGATGGACATTATTTC CGTGGTTACCTGGGCACACTGGTAATCTGGTCGACCGCCCTTTCACAAACCCACCTCCAGCACAGTCCCC TGCATCCAAGTGCAGCAGATGAGTTGACCACCCTGATCCTGACAGCCACTTTCGATCCTCTGATGGAACA GTGGGCTCCATTTAGAGATGACTTATATCCCCGGCTGGAGGTTCTCCAGGACTCTGAGTCACAGCCTGAG ATTCTGTCACCTTTACAACCCCCACCTTGTGGGCAAACAGCTTGTGACAATGTGGAACTCATCTCCCAGT ACAATAAGCATGGGCCCCTTCGTAGAGAGAAAGTGATCCGCTACCAGGTGGTAAACATCTATGATGATGA GGGTCTGCATCCCATTGTGAGTGATCATCAGATCCGCCGTCAGCATGAGGCCCTCAACCAGGCCTTCAGC CGCTACAATATCAGCTGGCAGCTGAGTGTCCACCGAGTCCACAACTCCACCCTGCGCCACCGGGTTGTGC TTGTTAATTGTGAGCCCAGCAAGATTGGCAATGACCACTGTGACCCTGAATGTGAGCATCCACTCACCGG CTTCGATGGGGGCGATTGCCGCCTGCAGGGACGCTGCTACTCCTGGAATCGCAGGGATGGCCTCTGTCAC GTGGAGTGCAACAACATGCTGAATGACTTCGATGATGGAGACTGCTGCGATCCGGAAGTGACTGATGTCC GAAAGACTTGCTTTGATCCTGACTCACCCAAGAGGGCATACATGAGTGTGAAGGAACTAAAGGAGGCCTT GCAGCTCAACAGCACTCACTTCCTTAATGTCTACTTCGCCAGCTCAGTCCGGGAAGACCTTGCAGGTTCT GCCACATGGCCCTGGGACAAGGAAGCTCTCAGTCACCTGGGTGGTGTTGTCCTCAACCCAACCTATTATG GCATGCTTGGCCACACCAACATCATGATCCATGAGGTGGGACATGTCCTGGGACTCTACCATGTATTCAA AGGAGTCAGTGAAAGAGAATCCTGTGATGATCCCTGTAGAGAGACAGTGCCATCCATGGAGACAGGAGAC CTGTGTGCTGACACTGCTCCCACACCCAAGAGCAAGCTGTGTCGGGACCCAGAGCCAGCTAATGACACCT GTGGCTTCACCCTCTTCCCAGGGGCTCCGTTCAATAACTACATGAGCTACACAGATGATGAGTGCACTGA CAACTTCACCCCTAACCAAGTGGCCCGGATGCATTGCTACTTGGACCTCGTATACCAGCAATGGAGTGAA AGCCAAAAACCTACCCCCATACCCATACCACCCATGGTTATTGGGCAAACCCACAAGTCCCTTACTATCC ATTGGCTGCCTCCAATCAGTGGAGTTGTGTATGATAGGGCCCCTGACAGCATGTGTAGTGCCTGCACTGA AGATGGGACATTCCGTCAGTATGTATACAAGGCATCCTCTGGGCGGGTGTGTGACTCTTCAGGTTACTGG ACTCCAGAGGAGGCTGTGGGGCCTCCTGATGTCGATCAACCCTGTGAGCCTAGCTTGCAGGCCTGGAGTC CTGAGCTCCACCTAAACCACATGAATATGACCGTGCCCTGCCCAGCAGAAGGCTGTAGCTTGGAGTTGCT TTTTCAATACCCGGTCCAGGCGGATACCCTCACCCTCTGGGTCACTTACCTCTCCATGAACTCTTCCAAG GCATTCTTTGACATAGAGATCTTGCTAGAACACAAAAAATCTATGCACCTTGGACCTTTAAACACTTTTT GTGACATACCGCTCACCATCAAACTCCACATGGATGAGAAGGTCATGGGAGCGAAAGTCTACACCTTTGA TGAACGAATGGAAATTGATGCTGCTCTCCTGACCTCTCGGCCCAATAGTTCCTTGTGTTCTGGATGCAAG CCTATGAGTTACCAGGTTCTTCGAGAGCCTCCTTTTTCCAGTGGCTTGCCCATGGTGGTGACACATCCAC ACAGAAAGTTCACAGATACGGAGGTCATCCCTGGGCAGATGTATCAGTACCAAGTTCAAGCTGAAGCTGT AGGGGAACTCGGAGAAGCTTCACCTCCTCTGAGCCACATTCACGGAGGCCCTTACTGTGGAGATGGGAAG GTAGAAAGGAGTATCGGAGAGATGTGTGATGATGGAGACCTGTTGAATGGAGATGGGTGCTCAAGAGCAT GTGAACTGGAGGAAGGCTTCAATTGTGCAGGGGAGCCAAGCCTTTGCTACAGGTATGAGGGAGATGGGGT TTGTGAAGATTTTGAGAAGGAAAGCATCATCACAGACTGTGGCCTCCACACTCCTGAAGGATATTTGGAT CAGTGGGCTAGCCAGGCTTATTCCTACCATGAAGACAAGGAGAAATGTCCTGTTTCCCTGGTAACAGGAG AACCTCATTCAATGATCTGTACTTCATGGCACCCGGATTCATCCCCATATCATTCCTTTCCTGGCTGGTT TCCCTGTGTCTTCAGTCTGAAGAAAGACCAGGATGCAGGCAGTGAGCAGGCTAAAGATAGCCTGCAGAAG GACAATGAAATTTGGCTTGAAGTGTGTTTCAATAGGCCAGGGGTTGCAGTGGCAATTTACATTTTCTTGG CATCGGATGGCGTGACCCCTGGGGAGCATCAGCAGGCAACAGTGACCCTTTACCTGATTGATGTCAGTGG AAGCAACCACTCTCTTGGAACCTATGGACTGTCGTGTCAACAAAATCCACTGGTTATCAATGTGAGCCAT CATGTGAATGTCTACCCCCAACATACCTCCTCCATGCTGCTGAATTTTTCATCCCCACTGGTGGGCATCT CAGCAGTGGCTCTAAGGACATCCTCCCAGACCAGTTCTTCAGCTCCCAGTAACTGCATCCCAGAGCAAAA TTATGAGGGAAAGAGCTGTGCTCAACGACCTTGTGGAGAACAGGGAAGATGTGCGCCATTGCTACTCGAT CATACGGACATGGTGAATTGTACCTCCAGTAGCCCAGGTCACATGGAGTGCGCTATCACTTGTCAAAGGG GGTATGTCCTTCAGACCAGCAGTGGGCAGTACCTGAGGAACATGCAGAAAGAGATTCTCCTTACTTGTTC CTCTGGCCACTGGGACAAGGATGTGATCTGCAAGCGTCTTGACTGTGGTGTCCCAGACTCCTCCTTGGTG AATTATGCAAACTTCTCCTGCTTGGAGGGTACAGACTTTCTGAAACAATGTTCCATCTCGTGTGTCCCAC CGGCTAAGCTCCAAGGACTGAACCCATGGCTGACATGTCTTGAAGATGGACTCTGGTCTCTCCCTGAAGT CTACTGCAAGTTGGAATGTGAAGCTCCTCCAGTTATTCCCAATGCCAATCTGCTCCTGCCACATTTCCTG GAAGGCAACCATGATGTGGGCACCATCTGCAAATATGAGTGCAAACCAGGCTACTATGTGAAGGAGACTT CAGGAAGTCAAGGCAAGAACAAGTTCCTGAAGATCCAGTGCCTGGATGATGGAAGCTGGGAACAAGGGAG CTGCGTCCCCGTGGTTTGTGAGCCTCCTCCTCCGGTGTTTGAAGGCATGTACGAATGTACTGATGGCTTC AAGCTGGACAGCCAGTGTGTGCTCAACTGTAACCAGGAAACGGAAGGGATTCCCATCCTCTGCACTAAAG AGGGGTTGTGGACCCAGGAATTCAAGTTGTGTGAAAACCTGCAAGGAGAATGCCCACCACCCACCTCAGA GCTGAATTCTGTGGAGTACAAGTGTGGACAGGGATATGGGATCGGTGCAGTGTGCTCCCCATCATGTGTA ATCCCCCCTAGTGACCCTGTGATACTACCAGAGAATGTGACTACTGACACTTTGGAGCACTGGATGGAAC CTGTCAAAGTCCAGAGTATCGTGTGTACCGGCAGGCGCCAGTGGCACCCAGATCCCTCTCTGGTGCACTG CATCCAGTCATGTGAGCCTTTCCAAGCAGATGGTTGGTGTGACACTATTAATAACCGGGCCTACTGCAAC TATGACGGGGGAGACTGCTGCTCTTCCACACTCTCTTCCAAGAAGGTCATTCCATTTGCTGCTGATTGTG ACTTGGATGAATGCACCTGTCGAGACCCCAAGGCAGAAGAAAACCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR226285 representing NM_001085376 Red=Cloning site Green=Tags(s) MMCWKVLRISLVVLTGWALSTTNSEQGWTRKKFLAQKEHLNQVLLEGERCWLGYKVRRPRTAPQHHLFGV YPSRLENYPRSYPVGTQQQTLYAEWSQRSPERQAPSGIPRDLTENPAGARRESQQPAAPWVGDGPIGQSQ LLRNDDTYLGEGGSKEALGEPAIQGSSEIAVSTIATFAYQKGPTAETQRKGQSKSRLPRQVQKRQAEDMT GDPQNTPQGFQLWPKEPLKHGDSDSPLEGTIQNGGGASLRRVETFNSQGGLPVLYFTGKRERLLLRPEVL AEIPREAFTVEAWVRPEGGQSNPAIIAGVFDNCSHTVNDKGWALGIRSGKDKGWRDARFFFSLRTDRMKK ATIVTGHSRYQPGMWTHVAATYDGQHTALYVDGTRVASSRDQFGPLNSPFMASCRSLILGGDSSEDGHYF RGYLGTLVIWSTALSQTHLQHSPLHPSAADELTTLILTATFDPLMEQWAPFRDDLYPRLEVLQDSESQPE ILSPLQPPPCGQTACDNVELISQYNKHGPLRREKVIRYQVVNIYDDEGLHPIVSDHQIRRQHEALNQAFS RYNISWQLSVHRVHNSTLRHRVVLVNCEPSKIGNDHCDPECEHPLTGFDGGDCRLQGRCYSWNRRDGLCH VECNNMLNDFDDGDCCDPEVTDVRKTCFDPDSPKRAYMSVKELKEALQLNSTHFLNVYFASSVREDLAGS ATWPWDKEALSHLGGVVLNPTYYGMLGHTNIMIHEVGHVLGLYHVFKGVSERESCDDPCRETVPSMETGD LCADTAPTPKSKLCRDPEPANDTCGFTLFPGAPFNNYMSYTDDECTDNFTPNQVARMHCYLDLVYQQWSE SQKPTPIPIPPMVIGQTHKSLTIHWLPPISGVVYDRAPDSMCSACTEDGTFRQYVYKASSGRVCDSSGYW TPEEAVGPPDVDQPCEPSLQAWSPELHLNHMNMTVPCPAEGCSLELLFQYPVQADTLTLWVTYLSMNSSK AFFDIEILLEHKKSMHLGPLNTFCDIPLTIKLHMDEKVMGAKVYTFDERMEIDAALLTSRPNSSLCSGCK PMSYQVLREPPFSSGLPMVVTHPHRKFTDTEVIPGQMYQYQVQAEAVGELGEASPPLSHIHGGPYCGDGK VERSIGEMCDDGDLLNGDGCSRACELEEGFNCAGEPSLCYRYEGDGVCEDFEKESIITDCGLHTPEGYLD QWASQAYSYHEDKEKCPVSLVTGEPHSMICTSWHPDSSPYHSFPGWFPCVFSLKKDQDAGSEQAKDSLQK DNEIWLEVCFNRPGVAVAIYIFLASDGVTPGEHQQATVTLYLIDVSGSNHSLGTYGLSCQQNPLVINVSH HVNVYPQHTSSMLLNFSSPLVGISAVALRTSSQTSSSAPSNCIPEQNYEGKSCAQRPCGEQGRCAPLLLD HTDMVNCTSSSPGHMECAITCQRGYVLQTSSGQYLRNMQKEILLTCSSGHWDKDVICKRLDCGVPDSSLV NYANFSCLEGTDFLKQCSISCVPPAKLQGLNPWLTCLEDGLWSLPEVYCKLECEAPPVIPNANLLLPHFL EGNHDVGTICKYECKPGYYVKETSGSQGKNKFLKIQCLDDGSWEQGSCVPVVCEPPPPVFEGMYECTDGF KLDSQCVLNCNQETEGIPILCTKEGLWTQEFKLCENLQGECPPPTSELNSVEYKCGQGYGIGAVCSPSCV IPPSDPVILPENVTTDTLEHWMEPVKVQSIVCTGRRQWHPDPSLVHCIQSCEPFQADGWCDTINNRAYCN YDGGDCCSSTLSSKKVIPFAADCDLDECTCRDPKAEENQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001085376
ORF Size	5367 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001085376.3
RefSeq Size	8602 bp
RefSeq ORF	5370 bp
Locus ID	23850
MW	199.3 kDa

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