Pcdh15 (NM_001142742) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	av; BB078305; ENSMUSG00000046980; Gm9815; nmf19; roda; Ush1f
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224928 representing NM_001142742 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCTACAGTTTGCTGTCTGGAAGTGTTTACCCCATGGGATCCTCATTGCCTCTCTCTTGGTAGTCA GCTGGGGCCAGTATGACGATGACTGGCAATACGAGGATTGCAAACTAGCTAGGGGAGGACCACCAGCTAC TATCGTGGCCATTGATGAAGAGAGTCGAAACGGTACAATTCTGGTGGATAACATGTTGATTAAGGGGACT GCCGGAGGACCAGACCCCACCATAGAGCTCTCTTTAAAGGACAACGTGGACTACTGGGTGTTGCTGGACC CCGTTAAACAGATGCTTTTCCTGAACAGTACCGGAAGAGTTCTGGATAGAGACCCACCAATGAACATACA CTCCATTGTGGTGCAAGTCCAGTGTGTCAACAAGAAGGTTGGCACAGTTATCTATCATGAAGTACGCATC GTGGTGCGAGATCGGAATGACAACTCCCCCACATTCAAGCATGAAAGCTACTATGCCACCGTGAATGAGC TCACTCCAGTTGGCACCACGATATTCACGGGGTTCTCGGGAGACAATGGAGCTACAGACATAGACGATGG CCCTAATGGACAGATAGAATACGTGATTCAGTACAACCCAGAAGATCCGACATCCAACGACACCTTTGAA ATTCCACTCATGCTGACTGGCAACGTGGTACTGAGGAAAAGACTCAACTATGAGGATAAGACTCGCTACT ATGTCATCATCCAAGCAAATGACCGTGCACAAAATCTGAATGAGAGGCGAACAACCACCACCACCCTCAC AGTAGATGTTCTAGATGGAGATGACCTGGGACCTATGTTTCTGCCTTGTGTTCTTGTGCCAAACACACGT GACTGTCGTCCACTCACCTACCAAGCTGCCATTCCTGAACTGAGGACTCCGGAAGAACTGAACCCTATTT TGGTGACACCACCTATCCAAGCCATTGATCAGGACCGAAACATCCAACCACCATCTGATCGACCTGGCAT CCTCTACTCCATCCTTGTCGGCACCCCTGAGGATTACCCCCGCTTCTTCCATATGCATCCCAGGACTGCA GAACTCACTCTCCTGGAGCCAGTAAACAGAGACTTCCATCAAAAATTTGATTTGGTTATTAAGGCTGAGC AGGACAATGGCCACCCACTTCCTGCCTTTGCTAGTCTGCACATCGAAATACTAGACGAAAACAATCAGAG TCCATACTTCACAATGCCCAGCTATCAAGGATACATCCTGGAATCCGCCCCAGTGGGAGCCACCATTTCT GAGAGCCTAAACTTAACCACTCCTCTGAGAATTGTAGCTCTGGACAAAGACATAGAAGACGTGCCACCTG GTGGAGTTCCTACAAAAGATCCAGAGCTCCACCTCTTCCTGAATGACTACACCTCGGTCTTCACTGTGAC ACCCACTGGTATCACCCGCTACCTCACCCTGCTTCAACCTGTGGACAGGGAGGAACAGCAAACCTACACC TTTCTGATAACAGCGTTTGATGGCGTGCAAGAAAGTGAGCCAGTCGTGGTCAATATCCGAGTGATGGATG CAAATGATAACACGCCCACCTTCCCTGAAATCTCCTATGATGTCTATGTTTACACAGACATGAGTCCTGG GGACAGCGTCATTCAGCTGACAGCGGTAGATGCTGATGAAGGCTCTAATGGGGAGATCTCCTATGAAATA CTGGTGGGGGGCAAGGGAGACTTCGTGATCAACAAGACCACAGGGCTGGTGAGCATTGCACCAGGCGTGG AGCTGATCGTGGGACAGACGTATGCGCTCACAGTGCAGGCTTCGGACAACGCCCCGCCTGCAGAAAGAAG GCACTCCATCTGCACAGTGTACATCGAGGTGCTTCCTCCTAACAACCAGAGCCCTCCCCGCTTCCCGCAG CTGATGTACAGTCTGGAAGTCAGCGAGGCCATGAGGATCGGTGCTATTTTATTAAATCTACAGGCAACTG ATCGAGAGGGAGATCCAATCACATATGCCATCGAGAATGGAGACCCTCAGAGAGTTTTTAATCTTTCAGA AACCACAGGGATTCTCAGCCTAGGGAAGGCTCTAGACCGCGAGAGCACAGACCGCTACATCCTCATCGTC ACAGCCTCAGATGGCAGACCGGATGGAACCTCAACTGCCACTGTGAACATAGTGGTGACGGACGTCAATG ACAACGCTCCCGTGTTCGATCCCTATCTGCCCAGGAACCTCTCTGTGGTGGAGGAAGAAGCCAATGCCTT TGTGGGTCAAGTCCGGGCAACAGACCCAGATGCTGGGATAAACGGCCAAGTTCACTACAGCCTGGGGAAC TTCAACAACCTCTTCCGCATCACATCCAACGGGAGCATTTACACAGCCGTGAAGCTGAACAGGGAAGCCA GGGACCACTATGAACTGGTTGTCGTGGCAACAGATGGAGCAGTCCACCCTCGACATTCAACTCTGACACT GTACATCAAGGTGTTGGACATTGATGATAACAGTCCTGTTTTTACCAATTCAACGTACACAGTTGTCGTT GAAGAGAATCTGCCAGCCGGGACCTCCTTTCTTCAAATAGAGGCCAAGGATGTTGACCTTGGAGCCAATG TGTCATATCGGATCAGAAGCCCAGAAGTGAAACACCTTTTTGCACTGCATCCATTCACTGGAGAATTGTC TCTTCTGAGGAGTTTGGATTATGAGGCCTTTCCGGACCAGGAGGCAAGCATCACATTCTTGGTGGAGGCC TTTGACATTTATGGGACTATGCCACCTGGTATAGCAACAGTCACGGTAATTGTGAAGGACATGAATGACT ACCCTCCAGTGTTTAGCAAACGCATCTACAAGGGGATGGTGGCTCCAGATGCAGTCAAGGGGACACCAAT CACCACCGTTTATGCTGAAGATGCGGACCCACCTGGGATGCCTGCAAGTAGGGTGAGGTATCGAGTGGAC GACGTGCAGTTTCCATACCCAGCCAGTATTTTTGATGTAGAGGAAGATTCTGGAAGAGTAGTAACCCGCG TCAATCTTAATGAAGAGCCTACTACGATTTTCAAGCTGGTGGTTGTGGCTTTTGATGACGGCGAACCTGT GATGTCCAGCAGTGCCACGGTGAGAATTCTTGTCTTACATCCTGGAGAGATCCCACGCTTCACCCAAGAG GAATACAGACCTCCTCCTGTAAGTGAGCTTGCGGCCAGAGGGACTGTAGTTGGTGTCATTTCTGCTGCTG CCATTAATCAGAGCATCGTGTACTCCATTGTGGCAGGAAATGAGGAAGACAAGTTTGGAATCAACAATGT CACTGGGGTCATCTATGTGAATTCACCATTGGATTACGAGACAAGGACCAGCTATGTGCTCCGGGTACAA GCAGATTCTCTGGAAGTGGTCCTTGCCAATCTCCGAGTCCCTTCAAAAAGCAATACAGCTAAGGTGTACA TTGAGATTCAGGATGAAAACGATCACCCCCCAGTGTTCCAGAAGAAATTCTACATTGGAGGTGTGTCTGA AGACGCAAGGATGTTCGCATCTGTGCTCAGAGTGAAGGCCACCGACAGGGACACGGGTAATTACAGTGCC ATGGCCTACCGGCTCATCATACCGCCGATTAAAGAGGGCAAAGAGGGGTTTGTGGTGGAAACATACACAG GTCTCATCAAGACAGCCATGCTCTTCCACAATATGAGAAGATCCTACTTCAAGTTTCAAGTGATTGCAAC TGACGACTACGGGAAGGGGTTGAGCGGGAAAGCAGACGTACTGGTCTCCGTGGTCAATCAACTGGATATG CAGGTCATTGTCTCCAATGTGCCCCCTACACTAGTGGAAAAGAAGATAGAAGACCTTACAGAGATTTTGG ATCGCTACGTTCAGGAGCAAATTCCTGGTGCCAAGGTTGTGGTGGAGTCCATAGGTGCCCGTCGCCATGG AGACGCCTACTCCCTAGAAGACTATAGCAAGTGCGACCTGACTGTCTATGCCATCGACCCGCAGACCAAC AGAGCCATCGACAGAAATGAGCTTTTTAAGTTCCTGGACGGCAAACTGCTCGATATCAATAAAGACTTCC AGCCGTATTACGGGGAAGGAGGGCGCATTCTGGAGATTCGGACACCTGAGGCAGTGACGAGCATCAAGAA GCGAGGAGAAAGCTTGGGGTACACAGAAGGGGCCTTGCTGGCCTTGGCCTTCATCATCATCCTCTGTTGC ATCCCAGCCATCTTGGTCGTCTTAGTAAGCTACCGACAGTTTAAAGTACGCCAGGCTGAGTGCACGAAGA CCGCAAGAATTCAGTCTGCTATGCCTGCAGCCAAGCCTGCAGCTCCTGTACCAGCTGCGCCTGCGCCGCC CCCGCCCCCGCCACCACCACCACCAGGAGCACATCTCTATGAAGAACTGGGAGAGAGCGCAATGCATAAG TATGAGATGCCCCAGTATGGAAGTCGCCGTCGACTGCTGCCACCTGCTGGACAGGAGGAATACGGCGAAG TCATTGGTGAAGCTGAAGAGGAATATGAAGAAGAAGAGGTAGAGCCAGAGAAAGTTAAAAAACCCAAAGT TGAAATTAGAGAGCCTAGTGAGGAGGAGGTGGTAGTCACCGTTGAGAAGCCACCAGCGGCTGAGCCCACA TACCCAACGTGGAAGAGAGCCAGGATATTCCCGATGATTTTTAAGAAAGTCAGAGGTCTCGCTGAGAAAA GAGGCATTGACCTTGAGGGCGAGGAGTGGAGGAGGCGCCTTGATGAAGAAGACAAAGACTATCTTCAACT GACTCTAGACCAGGAGGAAGCTACCGAAAGCACCGTGGAGTCAGAGGAGGAGTCCAGCGACTACACAGAA TACACAGAAACGGAGTCCGAGTTCAGCGAGTCCGAGACAACTGAAGAATCAGAGTCGGAGACCCCATCTG AGGAAGCGGAGGAGAGCTCTACCCCGGAGTCAGAGGAGTCTGAGTCCACTGAGTCAGAGGGAGAGAAAGC AAGAAAAAACATCGTGCTAGCTAGAAGAAGGCCTGTGGTCGAGGAAATCCAGGAGGTGAAAGGTAAGAGA GAGGAGCCCCCGGTGGAAGAGGAAGAAGAGCCCCCACTAGAGGAGGAAGAACGGGCAGAGGAAGGAGAAG AAAGCGAAGCAGCTCCCATGGATGAGTCCACAGACCTGGAGGCTCAGGATGTCCCAGAGGAGGGCAGTGC AGAATCAGTCTCCATGGAGAGGGGCGTGGAAAGTGAGGAGTCAGAGTCAGAACTGAGCAGCAGCAGCAGT ACCAGTGAGAGTCTCTCCGGAGGCCCCTGGGGCTTTCAGGTGCCAGAATATGACAGAAGGAAGGATGAAG AGCCCAAGAAATCTCCAGGCGCAAACTCCGAAGGTTACAACACAGCCCTTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001142742
Insert Size	5373 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001142742.1, NP_001136214.1
RefSeq Size	6303 bp
RefSeq ORF	5373 bp
Locus ID	11994
UniProt ID	Q99PJ1
Gene Summary	Calcium-dependent cell-adhesion protein. Required for inner ear neuroepithelial cell elaboration and cochlear function. Probably involved in the maintenance of normal retinal function.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (I) includes an alternate in-frame exon in the central coding region and has a distinct 3' splice pattern, compared to variant A. The resulting isoform (CD2-1), also known as protocadherin-15-CD2 isoform 1, includes an alternate 7-aa segment in the central coding region and has a distinct and longer C-terminus, compared to isoform CD1-1.

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