Tsc2 (NM_011647) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Nafld; Tcs2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224927 representing NM_011647 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAAACCAACAAGCAAAGATTCCGGCTTGAAGGAGAAGTTCAAGATACTGTTGGGATTGGGAACAT CGAGGCCAAATCCCAGGTGTGCAGAAGGCAAACAGACTGAGTTTATCATCACATCGGAAATCTTGAGAGA ACTGAGTGGTGAATGCGGCCTCAACAATCGCATCCGAATGATAGGGCAGATCTGTGATGTGGCAAAAACT AAGAAGCTTGAGGAGCATGCAGTGGAGGCACTTTGGAAGGCTGTCTCAGACTTGCTACAGCCAGAGCGGC CACCAGAGGCCCGGCATGCAGTTCTCACCTTATTGAAGGCCATTGTACAGGGACAGGGTGATCGTTTGGG AGTTCTCAGAGCCCTCTTCTTCAAAGTGATCAAGGACTACCCCTCCAATGAAGACCTCCATGAAAGGCTA GAAGTTTTTAAGGCCCTCACAGACAATGGGAGGCACATTACCTATTTGGAAGAAGAACTGGCAGAGTTTG TCCTGCAGTGGATGGATGTTGGCTTGTCCTCAGAATTCCTTCTGGTACTTGTCAACCTGGTCAAGTTCAA CAGCTGTTACCTTGACGAATACATTGCATCAATGGTTCACATGATTTGTCTGCTATGCATCCGGACAGTG TCCTCTGTGGACATTGAGGTGTCCTTGCAAGTGCTGGATGCTGTGGTCTGCTACAACTGCTTACCAGCCG AGAGCCTGCCTCTGTTCATTATCACCCTGTGCCGCACCATCAATGTCAAGGAGCTGTGTGAGCCTTGCTG GAAGTTGATGCGTAACCTTCTGGGCACCCACCTGGGCCACAGCGCCATCTACAACATGTGCCGCATCATG GAGGACAGATCCTACATGGAAGATGCCCCACTGCTGAGAGGAGCTGTGTTCTTTGTAGGGATGGCACTCT GGGGAGCTCACCGGCTCTACTCTCTCAAGAACTCCCCCACATCTGTGCTGCCGTCTTTTTATGAGGCTAT GACCTGTCCCAATGAGGTGGTGTCATATGAGATTGTTCTGTCCATAACAAGACTCATCAAGAAGTATAGG AAGGAGCTCCAGGCTGTGACATGGGATATTCTGCTGGACATCATTGAACGACTACTTCAGCAACTCCAGA ACCTGGACAGCCCGGAACTCAAGACCATCGTCCATGACCTGCTGACCACTGTGGAGGAGCTATGTGACCA GAACGAGTTCCATGGCTCGCAGGAAAGATACTATGAACTGGTGGAGAGCTATGCAGACCAGAGACCTGAA TCCTCTCTCTTAAACTTGATATCCTACAGAGCCCAGTCCATCCACCCTGCCAAGGATGGCTGGATCCAGA ACTTGCAGTTGTTGATGGAGAGGTTCTTCAGGAATGAGTGCCGCAGCGCCGTGCGCATCAAGGTGTTGGA TGTTCTGTCCTTCGTGCTGCTGATCAACAGGCAGTTCTATGAGGAGGAGCTGATTAACTCGGTGGTCATC TCGCAGCTCTCCCACATTCCCGAGGATAAGGACCATCAGGTCCGAAAGCTGGCTACTCAGCTGCTGGTGG ACCTGGCAGAGGGGTGCCACACCCACCACTTCAACAGTCTGCTGGACATCATTGAAAAGGTGATGGCGCG CTCACTCTCTCCACCCCCGGAGCTGGAAGAAAGGGACCTGGCCATGCACTCGGCCTCCCTGGAGGACGTG AAGACCGCGGTCCTGGGGCTCCTGGTCATCCTTCAGACCAAGCTCTACACCTTGCCTGCCAGCCACGCCA CTCGAGTGTATGAGAGCCTCATTAGTCACATCCAGCTCCATTACAAGCACGGCTACTCCCTGCCCATTGC TAGCAGCATCCGACTACAGGCCTTTGACTTCCTGCTGCTACTGCGGGCTGACTCGCTGCATCGACTGGGC CTGCCCAACAAGGATGGGGTCGTGAGATTCAGCCCTTACTGCCTCTGTGACTGCATGGAACTGGATAGAG CCTCAGAGAAGAAAGCCAGTGGGCCCCTTTCACCTCCAACTGGGCCCCCTAGCCCTGTGCCTATGGGCCC TGCTGTGCGGCTCGGCTACCTACCCTACTCCCTGCTCTTCCGTGTCCTGTTGCAGTGTTTGAAGCAGGAG AGCGACTGGAAGGTGCTGAAGCTGGTGCTCAGCAGGCTGCCGGAGTCACTGCGCTACAAAGTCCTCATCT TCACCTCCCCCTGCAGTGTCGACCAGCTGTCTTCTGCCCTCTGCTCCATGCTTTCAGCTCCAAAGACCCT TGAGCGGCTCCGAGGTACCCCAGAAGGCTTCTCGAGAACTGACCTGCACTTGGCTGTGGTTCCCGTGCTA ACAGCATTAATATCTTATCACAACTATTTGGACAAGACCAGACAGCGTGAGATGGTGTACTGCCTGGAAC AAGGCCTTATCTACCGCTGCGCCAGCCAGTGCGTGGTAGCCTTGGCCATCTGCAGTGTGGAGATGCCCGA CATCATCATCAAGGCGCTTCCTGTCCTGGTGGTGAAGCTCACACACATTTCTGCCACAGCCAGCATGGCC ATCCCGCTCCTGGAGTTCCTGTCTACTTTGGCCAGGCTGCCCCACCTCTACAGGAACTTTGCTGCAGAGC AGTATGCCAGCGTGTTTGCCATCTCCTTGCCATACACCAACCCCTCCAAGTTCAACCAGTACATTGTGTG TCTGGCTCACCACGTCATAGCCATGTGGTTCATCAGGTGCCGACTGCCCTTTCGGAAGGATTTCGTCCCT TATATCACTAAGGGTTTGCGTTCCAATGTCCTCCTGTCTTTTGATGATACCCCTGAGAAGGACAGTTTCA GAGCACGGAGCACCAGTCTTAATGAGAGACCCAAAAGTTTGAGGATAGCCAGAGCCCCCAAACAAGGCCT GAATAACTCTCCACCTGTGAAAGAATTCAAAGAGAGCTGTGCAGCCGAGGCCTTCCGGTGCCGCAGCATC AGTGTATCTGAACATGTGGTCCGCAGCAGAATACAGACATCCCTTACGAGCGCCAGCCTGGGGTCTGCGG ATGAGAACTCTATGGCCCAGGCTGATGACAACTTGAAGAATCTCCACTTGGAGCTCACAGAAACATGTCT GGACATGATGGCCCGATATGTGTTCTCCAATTTCACTGCAGTCCCCAAGAGGTCCCCTGTGGGAGAGTTC CTCCTGGCAGGGGGTAGGACCAAAACCTGGCTGGTTGGAAACAAGCTTGTCACTGTGACAACAAGTGTGG GAACTGGTACACGGTCGCTGCTGGGCCTGGACTCTGGGGACCTGCAGGGTGGCTCGGATTCAAGCTCTGA TCCTAGCACACATGTGAGGCAGACAAAGGAAGCACCGGCCAAACTGGAGTCCCAGGCTGGGCAGCAGGTG TCCCGTGGGGCCCGGGACCGGGTCCGCTCCATGTCAGGGGGCCATGGCCTTCGAGTTGGTGTCCTGGATA CTTCAGCTCCCTATTCCCCAGGTGGCTCTGCTTCTCTGGGACCACAGACGGCGGTGGCAGCGAAGCCCGA GAAGCCCCCTGCAGGAGCCCAGCTTCCAACAGCAGAGAAGACGAATCTGGCAGCCTATGTGCCTTTGTTA ACCCAGGGCTGGGCAGAAATCTTAGTCCGCAGACCCACAGGAAACACCAGCTGGCTGATGAGCTTGGAGA ACCCGCTCAGCCCCTTCTCCTCAGACATCAACAACATGCCCCTGCAAGAGCTGTCCAATGCCCTTATGGC TGCTGAACGTTTCAAGGAGCACCGGGACACGGCCCTGTACAAGTCATTGTCAGTGCCAGCGGCTGGCACC GCCAAGCCTCCTACTCTCCCACGCTCTAATACAGACTCAGCCATGGTCCTTGAGGAGGGAAGTCCAGGAG AGACTCAGGTGCCAGTGGAGCCCCCCGAATTGGAGGATTTCGAGGCGGCGTTAGGTACAGACAGGCACTG CCAGCGGCCTGACACCTACAGCCGGTCATCCTCAGCATCTAGCCAGGAAGAAAAGTCCCATTTGGAGGAG CTTGCTGCAGGGGGTATCCCCATTGAGCGGGCCATCTCCTCTGAGGGGGCTCGGCCTGCCGTGGACCTCT CCTTCCAACCCTCACAGCCTTTGAGCAAGTCTAGCTCTTCTCCGGAGTTGCAGACCTTACAGGACATCCT TGGAGACCTAGGGGACAAGATTGATATTGGACGGCTGAGTCCTGAGGCTAAGGTCCGGTCCCAGTCAGGG ATCCTGGATGGGGAAGCAGCTACCTGGTCAGCTACGGGTGAAGAGAGCCGTATCACAGTCCCACCTGAAG GTCCTCTGCCTTCCAGTTCTCCCCGCTCCCCCAGTGGCCTCCGGCCCCGAGGCTATACCATCTCTGATTC CGCTCCATCACGAAGGGGAAAGAGGGTAGAAAGGGATAACTTCAAGAGCAGAGCTGCGGCCTCCAGTGCT GAGAAGGTGCCAGGCATCAACCCTAGCTTTGTGTTCCTACAGCTCTACCATTCGCCCTTTTTTGGTGATG AGTCCAATAAGCCCATCCTATTGCCCAATGAGTCCTTTGAACGGTCAGTACAGCTGCTTGACCAGATTCC ATCCTATGACACTCACAAGATTGCTGTCCTGTATGTGGGAGAAGGCCAGAGCAGCAGTGAGCTGGCCATC CTGTCAAATGAGCATGGCTCTTACAGGTACACGGAGTTTCTGACAGGCCTGGGTCGGCTTATTGAGCTCA AGGACTGCCAACCAGACAAGGTGTACTTAGGTGGACTGGATGTATGTGGCGAGGATGGACAGTTCACCTA CTGCTGGCATGATGACATCATGCAAGCTGTTTTCCACATTGCCACCCTGATGCCCACCAAGGATGTGGAC AAGCACCGCTGTGACAAGAAACGGCACCTGGGCAATGACTTTGTTTCTATCATCTACAATGATTCTGGTG AGGACTTCAAACTGGGCACCATTAAGGGCCAGTTCAACTTTGTCCATGTGATCATCACACCGCTGGACTA CAAGTGCAACCTATTGACCCTGCAGTGCAGGAAAGACATGGAAGGCCTCGTGGACACCAGTGTGGCCAAG ATTGTGTCTGACCGCAACCTGTCCTTTGTGGCCCGACAGATGGCCCTGCATGCAAATATGGCCTCACAGG TACACCACAGCCGATCCAACCCCACTGACATCTATCCCTCCAAGTGGATCGCAAGACTCCGCCACATTAA GCGTCTCCGCCAGAGGATCCGTGAAGAGGTGCACTATTCCAACCCAAGCTTGCCTCTGATGCACCCTCCA GCCCACACCAAAGCCCCAGCTCAAGCCCCTGAGGCTACACCCACCTATGAAACAGGCCAGCGGAAGCGCC TCATTTCCTCCGTGGATGACTTCACAGAGTTTGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_011647
Insert Size	5358 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_011647.3, NP_035777.2
RefSeq Size	6266 bp
RefSeq ORF	5358 bp
Locus ID	22084

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