Ppl (NM_008909) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AW553870
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224905 representing NM_008909 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACTCGCTCTTCAGGAAGAGAAACAAAGGCAAATACAGCCCAACGGTGCAGACGCGGAGCATCTCCA ACAAGGAACTCTCAGATCTGATTGAGCAGCTGCAGAAGAATGCAGACCAGGTGGAAAGGAACATTGTGGA CACCGAGGCCAAGATGCAGAGCGACTTGGCCCGGATGCAAGAGGGGCAGCTCCCTGAGCACCGGGACGCG GCCCTGCAGAACGTGTCTGACTCAGAAAAGCTGCTGTACGTGCTGGAGGCAGATTCGGCCATCGCCAAGC ACATGAAGCACCCACAGGGGGACATGATCGCCGAGGACATCCGCCAGCTGAAAGAGCGCGTGACCAACCT GCGGGGGAAACACAAGCAGATGTACAGCCTGGCAGTGAAAGAGGCCGACCCGAGAGTCAACTGGGACACA CTGGTGGACGAGAAGCTGGACAAGCTGAGCAGCCAGAGCTTTGGGACTGACCTTCCTCTGGTGGATAGCC AGGTGGAGCAACACAACATCTTCCACAATGAAGTCAAAGCCATCGGGCCCCACCTGGCCAAGGACAAGGA GCAGAACAGTGAACTCCAAGCCAAGTATCAGAAGTTGCTGACAGCATCACAGGCACGGCAGCAGCACCTG AGCTCGCTGCAGGATTACATGCAGCGCTGCACCAATGAGCTGTACTGGCTGGACCAGCAAGCCAAGGGCC GCATGCAGTATGACTGGAGCGACCGCAACCTCGACTACCCCAGCCGCCGCCGCCAGTACGAGAATTTCAT CAACCGGAACCTGGAGGCCAAAGAAGAAAGAATCAACAAACTGCACACTGAAGGTGACCAACTGCTGACA GCTGAGCACCCAGGGAGGAACTCCATTGAGGCACACATGGAGGCTGTGCATGCTGAGTGGAAGGAGTACC TGAACCTGCTCATCTGTGAGGAGAGCCATCTGAAATACATGGAAGACTACCACCAATTTCACAAAGACAT GAAGGATGCTCAGGAGCTGCTACGCAAGGTGGACTCAGACTTGAACCAGAAATATAGCCCTGACTTCAAA GACCGGTATCAGATAGAGCTGTTGCTTCGGGAACTGGATGACCAGGAGAAGGCTCTGGACAAGTATGAGG ATGTGGTGCGAGGGCTGCAGAGGCGAGGGCAGCAAGTGGTGCCACTCAAGTACCGCCGCGAGACGCCGCT CAAGCCCATCCCTGTGGAGGCTCTCTGTGACTTTGAGAGTGACCAGGGCCTCATCTCGCGGGGCTATAGC TACACACTGCAGAAGAACAACGGGGAGAGCTGGGAGCTGACAGACAGCACTGGGAAGAAGCTGGCTGCCC CAGCCGTCTGCTTCATCATTCCCCCCACCGACCCCGAGGCCTTGGCTCTTGCTGACAGCCTGGGCAGCCA GTACCGAAGTGTGAGGCAGAAGGCGACTGGGAGCAAACATGCACTCCAGCAGCGGCATGAGGTGCTAAGG ACGGAGAACCCTGGAGATGCCTCTGATCTTCAGGGACGACAGCTGCTGGCTGGCTTGGACAAAGTGGCCA GTGACCTGGACCGGCAGGAAAAGGCTATCACAGGGATCCTACGGCCACCACTTGAGCAGGGACGGGCAAT TGAGGATAGTGCGGAGCGGGCCAAGGGTCTCAAGAACATTACCAATGAGCTGCTACAGATTGAGCCAGAG AAGACGCAGTGCACAGCCGAGTGTGAAGCCTTTGTGCAGGCCCTCCCGGCCAGCGGCACCGCACCCTTGC TGAAGACCCGGGTAGAAGACACCAACCAGAAATATGAGCGACTAGTATGGTTGCTAGAGGCAGCCCAGGA GAAGGTGGACGTGGCCAACCGCCTGGAGAACAGTCTGCAGCGGGGCAGGGAGCTGCTGGCCTCATACGAG AACAGGCTGATTCAGGACGACACAATGCCTGAAAGTGGTCATGTGTTGGACAGCAAGAGGCAGGAGCTGG AGGCCATGGCTTCTGAGCTGCAGGCCCACAAGTCCCTCCTGGGTGAGGTGGAACAGAACCTACAGGTGGC CAAGCAGTGTTCCAGCTCACTGGCCAGCCGCTTCCAAGAGCACTGCCCTGACCTGGAGCGGCAGGAGGCC GAGGTGCACAAGCTCAACCAACGTTTCAACAACCTCAGCCAGCAGGTGGAACGGAGGGCCCAGAGCCTGC AGAGTGCCAGGGCTGCCTACGATGAATACTGCAGTGGTTACAACCGTGTGCTCCAGTTTTTGGCCAAGAC TCCCAGTTATGAGCCCCAGGAGACAGACAGCCTCGGCCAGATGGAGACCAAGCTCAAGAACCAGAAGAAC TTGCTTGATGAGCTAGCAAGCAGGGAACAGGAGGTGCAGAAGGTCTATGCAGATTCCCAGCAGTACCAGC AAGCGGTGAAGGACTATGAACTTGAAGCAGAGAAGCTAAGGTCCTTGCTGGACCTGGAGAATGGACGGAA CAGCCATGTGAACAAGAGAGCCAGGCTGCAGTCTCCTGCTGCCAAAGTGAAGGAAGAGGAAGCTGCTCTT GCTGCCAAGTTCACAGAGGTTAATGCCATCAACAGACAGAGGCTACAGAATTTGGAGTTTGCACTGAATC TCTTGAGACAGCAACCAGAAGCAGGAGTGACCCATGAGACCCTACAAGGGGGAAAGCTAAGCTCTGGCAT GGAGGAAACATGGAAGATTAAGAAGGAACTGGAGGAGGAGATAGAGCGAAGGCAACAGCTGGAAAATGAG GTCAAGAGTGCCCAGGAGGAAATCCAGACCCTGAAGGATCAGGGGCCTCAGGAGTCATTGGTGAGGAAGG AGGTGCTGAAGAAGGTGCCAGACCCTGCCCTGGAGGAGAGCTTCCAGCAGCTGCAGCAGACGCTGGCTGA GGAACAGCACAAGAACCAGCTATTGCAGGAGGAGCTGGGAGCACTGCAGCTCCGGCTGCAGGCTCTAGAG CAGGAGACCAGGGATGGGGGACAGGAGTATGTGGTCAAGGAGGTCTTGCGCATTGAGCCAGACAGAGCCC AGGAGGATGAGGTCTTGCAGCTTCGGGAGGAGCTGGAGGCACTGCGGCGCCAGAAAGGCGCCCGAGAGGC TGAAGTGCTACTCTTACAGCAGCGTGTAGCAGCCCTGGCTGCTGAGAAGAGCAGGGTGCAGGAAAAGGTC ACTGAGAGAGAGGTGGTAAAGCTACAGAATGACCCCCAACTGGAGGCAGAATACCGGAGGCTGCAGGAGG AGCACCAGCGGGAGGGCACACTCAGGGAGAAGCAGGAGGAGGAGCTGAGTTTCCTACAGGCTAAGCTCAG GAGACTGGAGAAAGAACGAGCCATGGCAGAAGGCAAGATCACTGTCAAAGAGGTGCTCAAGGTAGAGAAA GATGCAGCTGTGGAGAGAGAAGTCAATGACCTCACCCGCCAGTATGAGGACGAGGCTGCCAAGGCACGCT CTGGCCAGCGGGAGAAGACAGAACTTCTCAGAAAGATATGGGCCCTGGAAGAGGAAAATGCTAAGGTGGT GGTACAGGAGAAGGTCCGAGAGATCGTGCGGCCAGATCCCAAGGCAGAGAGTGAAGTGGCCAACCTCCGC CTGGAGCTGGTGGAGCAGGAGCGCAAATTCAGGGGTGCTGAGGAGCAGCTGAAGAGCTACCAGAGTGAGC TGGAGGCCCTACGGAACCGTGGACCACAGGTAGAAGTCAAAGAGGTGACCAAAGAGGTCATTAAATATAC AACTGACCCAGAGACAGAGCAGGAACTCCAGAGGCTCAGGGAGGAGATCATGGACAAGACCAGGTTAATA GAAAGGTGTGACCTAGAGATTTACCAGCTGAAGCAGGAGATCCAGGCCCTTAAGGACACCAAGCCACAGG TGCAGACTAGAGAGGTAGTCCAGGAGATCCTGCAGTTCCAGGAAGACCCCCAAACCAAGAAGGAGGTAGA GTCTCTGCGTATACAGCTGTCAGAAGAACAGAAGAAACAGGTGGACCTGGAAGGGGAGCGGGCATCCCAG GAAGAGAAGATCAAACGCAAGGAGGAAGAGCTGGCACAGGGGAAGGAAAGGGTTGTACGCCAGGAGGTAG TACAGTATGAAGATGAGCCAGATTTGCGAGCTGAGGTGACTGCCTTCACAAACAGCATTGATGCTGAGCT GCGGCAGATTGATAAACTGCATGTGGAGCTACGGCGGTTACAGCATCGCCGAGCAGAGCTGGAGAGGCAG TTGGAGGAGCTGGAGCGGGAGAGGCAGGCACGTAGGGCAGCCGAGCTGGAGGTACAGAGGCTGCAACAGC GGCTGGCTGCATTGGAGCAGGAGGAGGCCAAGACTGGTGAGAAGGTGACCCACACACAAAAGGTGGTGCT GCAGCAGGACCCACAGCAGACCAGGGAGCATGCCCTGCTCCGAGCCCAGCTGGAGGAAGAGCGGCACCGG AGGCAGCTACTGGAGGGTGAGCTTGAGCCGCTGCGTAGAAAGTTGGCTGCCCTGGAGAAGGCAGAGATCA AAGAAAAGGTGGTCTTCTCTGAGAGTGTCCAGGTAGAAAAAGGTGACACTGAGCAAGAGATCCAGCGCCT CAAGAAGAGCCTGGAAGAGGAGAGCCAGAGCAAGAGGGAGCTGGATTCAGAGGTGACCCGGCTAGAAGCC AAGTTATCTGAGCTAGAATTCTATAATTCCAAGTCATCCAAGGAACTGGACTTCCTGAGAGAAGAAAACC ACAAGCTACAGCTAGAGCGGCAAAACCTGCAGCTAGAGACCCGGAGACTCCAATCAGAGATTGAGATGGC AGCAACAGAAACTCGAGATCTGAAGAACATAACCACGATAGACTCTGGGACCCACCTAAACTCTAGGCTG TGGTCCCTAGAGAAGGAACTGGATGACCTCAAGAAGATGTCCAAGGACAAGGATCTGGAGATCGATGAAC TACAGAGGCGCCTGGGCTCTGTGGCTGTCAAGAGAGAGCAGAGGGAGAACCACCTGAGGCGCTCCATAGT GGTCATAGACCCTGACACAGGTCGTGAGCTGTCCCCAGAGGAGGCTCACCGGGCTGGGCTTATCGACTGG AAAATGTTTGTGAAGCTCAGAAGCCAGGAGTGCGACTGGGAGGAGATATCAGTGAAAGGTCCTAATGGGG AGTCATCAGTAATTCATGACAGGAAGTCAGGCAAGAAGTTCTCCATTGAGGACGCTCTTCAGAGTGGCAG GCTAACCGCTGCTCAATATGACCGCTATGTCAACAAGGATATGTCCATTCAGGAGCTGGCAGTCTTGGTG TCTGGGCAGAAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI
ACCN	NM_008909
Insert Size	5265 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_008909.2, NP_032935.2
RefSeq Size	6277 bp
RefSeq ORF	5265 bp
Locus ID	19041
UniProt ID	Q9R269
Gene Summary	Component of the cornified envelope of keratinocytes. May link the cornified envelope to desmosomes and intermediate filaments. May act as a localization signal in PKB/AKT-mediated signaling.[UniProtKB/Swiss-Prot Function]

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