Marf1 (NM_001081154) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4921513D23Rik; BC031575; C87306; E030019O05; Lkap; mKIAA0430
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224895 representing NM_001081154 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGGGAAAGGAACTGAGAACCCTTGCAGCAGAACACTTGGATGGTTTCACCAAGATAATGATGCTA AGCCGTGGCTTTGGAAATTCTCTGGTTGCTTTTCTCGTCCCGAGCAGACATTGCCTCTTAGTTCCCAAAC GAAAGAGTACATGGAGAACAAGAAAGCTGCTGTGGAGTTAAAGGATATACCATCTCCCCTTCATGTTGGC TCTAAATTTTTCCCAGCAGTCCCACTTCCTGATATTCGTTCTCTTCAGCAACCTAAAGTACAGCTCTCAG CTATTCCCAAAGTAAGCTGCTGTGCTCATTGCCCTAATGAACCCTCCACTTCACCCATGCGTTTTGGTGG TGGCAGTGGCAGTAGTGGAGGTTCTGGGAGCTTGATTCACACGGGCTCACTCTTAGACTCACCAAGCACT GGGACAGTCACGTGTCAAGTAGGGTCAGGTTTTGCTTTCCAGTCCGTGTCTTCACTGCAGAATGCCTCCA CTAGGAACAACTTGGTGGGCCTTTCAAGTGACTTCCCCAGCATGTGTGTAGAGAGTAACCTACCTTCCTG CAAGCACCTGTCGTGTTGTGGGAAGCTCCATTTCCAGTCATGTCACAGTAATGTACACAAGCTGCATCAG TTCCAGAATCTTCAGGGCTGTGCCTCTGCTGGCTATTTCCCCTGTTCCGATTTCCCAAGTGGGGCTCCTG GGCATTTGGAAGAGCGCCTTTCCCATTCAGAGCTTACACCTCACTTGTGCACCAACTCTCTTCACCTAAA TGTGGCACCTCCGGTTTGTTTAAAGGGCTCACTTTACTGTGAAGACTGTCTAAACAAGCCAGCAAGAAAT AGTATAATTGATGCTGCTAAAATCTGGCCAAATATACCACCTCCAAGTACTCAGCCTGCACCTCCAGCTA TTCCTGTGTGTAATGGCTGTGGAACAAAAGGCATGGAAAAGGAGACCTCATTGTTGTTGGCAACCAGTCT AGGCAAAACTGCTTCGAAATTTGGGTCACCAGAAGTTGCAGTCACTGGACAGGTGCTGGAGACCCTCCCC CCCATTGGCGTTTTCTGGGATATTGAAAACTGCTCAGTCCCCTCTGGCCGGTCAGCAACAACTGTTGTAC AGAGAATCCGTGAGAAGTTTTTTAGAGGACACAGAGAAGCAGAGTTTATCTGTGTGTGTGACATCAGTAA AGAAAACAAGGAAGTTATTCAAGAGCTGAATAATTGTCAGGTAACCGTTGCTCACATAAATGCTACTGCA AAGAATGCTGCTGATGATAAACTCCGTCAGAGCCTGCGAAGGTTCGCAAACACGCACACTGCTCCAGCTA CTGTGGTTCTTGTGTCAACTGATGTCAATTTTGCGTTGGAACTCAGTGATCTTAGGCACCGGCACGGTTT CCACATCATTTTGGTACATAAAAACCAGGCCTCTGAAGCCTTGTTACATCATGCTAACCAGCTGATCAGA TTTGAAGAGTTCATTTCTGATCTCCCCCCTCGGTTACCACTGAAGATTCCTTGCCACACTCTACTCTATG TTTATAACCTGCCAGCAAATAAAGATGGCAAGAGCATCAGCAACAGGCTCAGGCGCCTCTCTGATAACTG TGGTGGCAAGGTGCTGAGTATCACAGGCTGCAGCGCAATTCTCCGCTTCATAAACCAGGACAGTGCAGAG CGGGCCCAGAAGCGAATGGAAAATGAAGATGTCTTTGGCAACAGGATCATCGTGTCCTTTACTCCAAAAC ATAGAGAATTCTTTGAAGCCAAGAGTTCAAATGCTATTGCCGACAAAGTGAAGTCTCCCAAAAAAGTTAA GAACACAAAATTGTGCCTCATCAAAGACACAAGTGAACAGTCACCCAGTGTCAAAGCCATGCCAGGGAAA GTGTTGCAGGCAAATTCTGGATCTGCTACAAAAACCACAAACATTAAAAGTTTACAGGAGCTGTGCCGAA TGGAGTCAAAGTCTGGCAATAGAAACAGTGACCATCAGCAAGGCCATGGGAGGCTGGCAGCACTGCCTAA CAGTGGTCCAACTGCTTCAGTGCCCATCGTGAAGAACACAGGTGTGACAGAGCCACTTTACAGAAGCAGT CAGAAAAAAGAGAACCCCAGTTCCCAGAGCACTGTCAACTCTCCTGTAGAGAAGAAGAAGAGAGAGGAGA CTGTCTTCCAAGTAAGCTACCCATCCGCATTCAGCAAGCTGATCGCCTCAAGGCAAGTCAGCCCTCTGCT CACCTCTCAGTCTTGGTCTCCGAGGAGTATGTCTCCAAACCTTTTGAATAGAGCCTCACCACTTGCTTTC AACATTGCCAATCCCAGCAGCGGTGCTGACTGCCCAGACCCATTTGCAAATGGTGTTGACATCCAGGTCA GCAATGTGGACTACAGGCTGTCCCGGAAGGAGCTGCAGCAGCTCCTCCAGGAAGCCTTTTCCAAGCATGG CCAGGTGAAGAGTGTTGAGCTCAGTCCCCATACTGATTACCAGCTCAAGGCCATTGTGCAGATGAGAAAC CTGCACGAAGCAATCTGTGCAGTGAACAGCCTCCATAGGCACAAAATTGGCAGCAAGAAGATACTGGTCT CACTCTCCACAGGAGCTGCCAACAAGTCACTCTCTTTACTAAGCACAGAAACAATGTCTATTCTTCAGGA TGCTCCTGCCTGTTGCTTGCCTCTCTTTAAATTTATAGACATCTATGAAAAGAAGTATGGACACAAGTTA AATGTGTCTGATTTATATAAATTAACAGACACTATTGCAATCCGTGAACAGGGAAACGGGCGATTGGTGT GTCTCTTACCTAGTAATCAGGCCCGCCAGAGCCCCCTGGGCTCTTCCCAGTCACATGATGGCTCCTCCTC AAACTGCAGCCCAGTTCTGTTTGAAGAGTTGGAGTATCACGAACCTGTCTGCAAGCAGCACTGCTCTAAC AAGGACTTCAGTGAACTTGTGTTTGATCCAGACTCTTACAAGATTCCTTTTGTGGTTCTCTCTTTGAAAG TGTTCGCACCTCAAGTTCATAGTCTTCTGCAGACCCACCAGGGCACTGTGCCTTTGCTAAGCTTTCCAGA TTGCTATGCTGCGGAGTTTGGTGACCTAGAAATTACGCAAGACAGCCACAAGGGTGTCCCCTTAGAGCAC TTCATCACCTGCATTCCAGGAGTGAACATTGCCACTGCTCAGAATGGCGTCAAAGTAGTGAAGTGGATTC ACAACAAGCCCCCACCTCCCAACACTGACCCTTGGCTTCTTCGTTCTAAAAGTCCTGTGAGAAATCCCCA GCTGATTCAGTTCAGCAGAGAGGTCATTGATTTACTGAAGACCCAGCCATCGTGTATACTACCCGTCAGC AATTTCATCCCTTCATACCATCACCACTTTGGAAAGCAGTGCAGGGTTTCAGACTATGGTTATTCCAAGC TGATTGAACTCCTAGAAGCTGTGCCTCATGTGTTGCAGATTCTTGGTATGGGCTCCAAGCGTCTGATGAC CCTTACTCACAGGGCCCAGGTGAAGCGCTTTACTCAGGATTTACTAAAACTTCTTAAGTCCCAGGCCAGC AAGCAGGTCATCGTGAGAGACTTTTCACAGGCTTATCATTGGTGTTTCTCAAAGGACTGGGATGTCACGG AATATGGTGTTTGTGACTTGATTGATATAATATCAGAGATCCCAGACACAACTATTTGCTTGTCCCAACA AGATGACGACATGGTCATTTGTATCCGAAAAAGAGAACGAACTCAGGATGAAATAGAAAGGACAAAGCAG TTCTCCAAAGATGTTGTGGACTTGCTGCGCCACCAGCCCCATTTCAGAATGCCCTTCAACAAGTTCATTC CCTCTTACCACCACCACTTTGGCCGACAGTGTAAACTTGCATACTATGGATTTACCAAGCTGCTTGAACT TTTGGAAGCCATACCTGAGATTTTACAAGTGTTGGAATGTGGAGAAGAAAAAATCCTGACTTTGACTGAA GTGGAGCGATTCAAAGCTCTAGCCGCCCAGTTTGTTAAGCTTCTGCGGTCCCAAAAGGGTAACTGCCTTA TGATGACAGATCTGTTTACAGAATATGCTAAAACTTTTGGTTATACATTTCGTCTCCAAGACTATGATGT CAGTTCAGTCTCAGCTTTGACACAGAAGCTCTGCCATGTTGTAAAGGTTGCTGATATGGAGTCTGGTAAA CAGATCCAGCTGATCAATAGGAAGTCTCTGCGGTCTCTCACTGCTCAGTTGCTGGTCTTGTTGATGTCTT GGGAAGGGGATGCTTATCTTCCTGTTGATGAGCTCAGGAGACATTATGAAACCACTCACAGCACTCCTCT CAACCCCTGTGAATATGGATTCATGACCCTGACTGAGCTACTGAAGAGTCTGCCGTACTTAGTAGAGGTT TGTACGAACGATAAGGCAGAAGAGTATGTGAAGCTCACAAGCCTGTACTTGTTTGCAAAGAATGTGAGGT CTTTGCTTCATACCTATCACTACCAGCAGATTTTTCTTCATGAATTTTCCATGGCCTATAACAAATATGT TGGAGAAACACTACAGCCCAAAACTTATGGCTACAGTAGTGTGGAGGAATTACTGGGAGCCATCCCACAG GTGGTCTGGATAAAAGGACATGGTCATAAGAGAATTGTAGTGTTAAAAAATGACATGAAAAACCGCCTAA GCTCATTTGACCTTTTCTCTGTCAATCATGAAGACCAGCCTGCAGTGAGCAGGCAGATCCTGGCGGTGCC AGAATCCCATCTAGCCTCAGAACTCCAGCTCCGAACAGGACCCAGCCAGATGGAGCAGGAGCTTCTGCAC CTGGCCAGCAGCTCCCCCGTTGACCTGCTCTGTGCTCCTGTTCCTTCATGCCTGCCATCCCCTCAGCTAA GACCAGATCCTGTCATCCTGAAGCCCGCTGATCTCATTCAGTTTGAGGAACACCCTCAAGAGCCTTTGGG AGTTTTAGTTTTAAACCAAGAAGAAAAATCAGAAGTTCCACTACCAGTGCAAAAGGGAAACCTGTCCTGT GACTCCTCACCCAGCAGCCCAGCAGCTTCTCCTGCTCCTCCTGGCCCCTCCTCAGAAGCCCCCAGGCCAC TTTTCAGCAAGGATGCAGTGGAAAGCCCAGCCAAAAAGCAGCCCAAAAATAGAGTCAAATTGGCAGCCAA CTTTTCTTTTGCACCTGTAACCAAGCTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001081154
Insert Size	5211 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001081154.2, NP_001074623.1
RefSeq Size	7765 bp
RefSeq ORF	5211 bp
Locus ID	223989
UniProt ID	Q8BJ34
Gene Summary	Essential regulator of oogenesis required for female meiotic progression to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Probably acts via some RNA metabolic process, equivalent to the piRNA system in males, which mediates the repression of transposable elements during meiosis by forming complexes composed of RNAs and governs the methylation and subsequent repression of transposons. Also required to protect from DNA double-strand breaks.[UniProtKB/Swiss-Prot Function]

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