Xrn1 (NM_011916) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Dhm2; exo; mXrn1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224877 representing NM_011916 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGAGTCCCCAAGTTTTACCGATGGATCTCGGAGCGGTACCCCTGCCTCAGCGAAGTGGTGAAGGAGC ATCAGATTCCAGAATTTGATAACTTGTACCTGGATATGAATGGGATTATACATCAGTGTTCCCATCCTAA TGATGATGATGTTCACTTCAGAATTTCAGATGACAAAATCTTTACTGACATTTTTCACTACTTGGAGGTG TTGTTTCGAATTATTAAACCTAGGAAAGTGTTCTTTATGGCTGTTGACGGTGTGGCTCCTCGAGCAAAAA TGAACCAGCAACGCGGGAGGCGTTTTAGGTCAGCCAAGGAGGCAGAAGACAAAATTAAAAAAGCAATAGA AAAGGGAGAAACACTTCCTACAGAAGCCAGATTCGATTCCAACTGTATTACGCCAGGGACTGAATTCATG GCCAGATTGCATGAACATCTGAAGTATTTTGTAAATATGAAAATTTCCACAGACAAATCATGGCAAGGTG TTACCATCTACTTCTCAGGCCATGAGACTCCTGGAGAAGGAGAGCATAAAATCATGGAATTTATCAGATC CGAGAAAGCAAAGCCAGATCATGATCCAAACACCAGACATTGTCTTTATGGCTTAGATGCTGACTTGATC ATGCTTGGATTAACAAGTCACGAGGCACATTTTTCTCTCTTAAGAGAAGAAGTTCGGTTTGGTGGAAAAA AAACACAAAGGGTATGTGCACCAGAAGAAACGACATTTCACCTCCTACACTTGTCTTTAATGAGAGAGTA TATTGATTATGAATTTTCAGCATTAAAGGAAAAGATCACATTTAAATATGATATTGAAAAAATTATAGAT GATTGGATTTTGATGGGATTTCTTGTTGGCAATGATTTTATCCCTCATCTACCTCATTTACATATTAATC ATGATGCGCTGCCTCTTCTTTATGGAACATATATTGCTATCCTGCCAGAACTTGGAGGTTATATTAATGA AAGTGGACATCTCAACTTACCTCGATTTGAGAGATACCTTGTGAAACTATCAGATTTTGATCGAGAACAC TTCAGTGAAGTTTTTGTGGACCTCAAGTGGTTTGAAAGCAAAGTTGGTAACAAGTATCTCAATGAGGCAG CAGGTGCAGCAGCAGAAGAGGCAAAGAACTGCAAGGAGAAGAGAAAACCGAAGGGTCAGGAAAATTCTTT AAGTTGGGCTGCATTAGACAAAAGTGAAGGTGAAGGAGTAGCTTCTAGAGATAATTTTGAGGATGAGACT GAAGATGATGATCTGTTTGAAACTGAATTTAGACAATATAAGAGAACATACTACATGACGAAGATGGGCG TCGATGTAGTATCTGATGAGTTTCTGGCTAATCAGGCTGCCTGTTATGTTCAGGCTATACAGTGGATCTT ACACTATTACTATCATGGAGTCCAGTCCTGGAGCTGGTACTATCCCTATCATTACGCACCCTTCCTATCT GATATCCGCAGTATCAGTACACTCAAAATCCATTTTGAACTCGGAAAACCCTTCAAGCCATTCGAGCAGC TGCTTGCTGTACTTCCATCGGCTAGTAAAAATTTGCTTCCTACATGCTACCAGCATTTGATGACCAGTGA AGACTCGCCAATTATAGAATATTATCCACCTGATTTCAAAACTGATCTAAATGGGAAACAACAGGAATGG GAAGCTGTGGTATTAATACCGTTTATTGATGAGACGCGATTATTGGAAGCCATGGAGACATGTAACCACT CCCTCAAAAAAGAAGAGAGGAAAAGAAACCAGCACAGTGAGTGCCTGATGTGCTGGTATGACAGAGACAC AGAGTTCACCTACTCCTCTCCATGGCCAGAAAAGTTCCCTGCCATAGAACGATGTTGTACAAGGTATAAA ATGATATCATTAGATGCTTGGCGCGTAGACATAAACAAGAACAAAATAACCAGGGTTGACCAGAAGGCAT TATATTTCTGTGGATTCCCTACTTTGAAACACATCAAACATAAATTTTTTTTGAAGAAAAGTGGTGTTCA AGTATTCCAGCAGAGTAGTCGTGGTGAAAACTTGATGTTGGAAATTTCAGTAAATGCAGAACCAGATGAA CTTAGGATAGAGAACATAGCTTCGGCGGTGCTGGGAAAGGCAGTCTTTGTTAACTGGCCTCATCTTGAAG AAGCTAGAGTTGTGGCAGTATCAGATGGTGAAACTAAGTTTTACATAGAAGAACCTCCAGGAACACAGAA GGTTTATTTGGGAAAAACTGCCCCACCATCTAAAGTCATCCAACTTACAGATAAAGAACAATCGAACTGG ACTAAAGAAATACAAGGAATTTCAGAACAATATCTAAGAAGAAAGGGAATAATAATAAATGAAACATCTG CTGTTGTGTATGCTCAGTTGCTCACGGGTCGTAAATATCAAATCAGTCAGAATGGTGAAGTTCGTCTAGA AAAACAGTGGTCAAAACAAATTCTTCCTTTTGTTTATCAAACTATTGTTAAGGACATCCGAGCTTTTGAC TCTCGTTTCTCCAATATCAAGACATTGGATGACTTGTTTCCACCGAGAACTATGGTCTTTATGCTTGGAA CTCCTTATTATGGCTGCACTGGAGAAGTTCAGGATTCAGGAGACTTGATTACAGAAGGGAGAATTCGTGT GGTTTTCAGCATTCCATGTGAGCCAAATCTTGATGCTTTAATACAGAATCAGCATAAATACTCTATAAAG TACAACCCAGGATATGTGTTGGCCGGTCGCCTTGGCGTGAGTGGATACCTTGTTTCAAGGTTTACAGGAA GTATTTTTATTGGAAGAGGATCTAGGAGAAACCCTCATGGAGACCATAAAGCAAATGTGGGTTTAAATCT CAAATTCAACAAGAAAAATGAGGAAGTCCCTGGCTATACAAAGAAAGTGGGGAACGAGTGGATGTACTCG TCGGCAGCAGAGCAGCTCCTCGCAGAGTACATAGAGAGAGCACCAGAACTGTTCAGTTATATAGCAAAAA ACAGCCAAGAAGATGTATTCTATGAAGATGACATCTGGCCTGGAGAAAATGAGAACGGGGCTGAGAAAGT TCAAGAAATTATTACTTGGCTAAAAGGACATCCTGTCAGTACTCTGTCTCGGTCTTCTTGTGACTTGCAC ATTCTGGATGCAGCCATTGTTGAAAAAATCGAGGAAGAAGTTGAGAAGTGCAAGCAAAGAAAGAGCAACA AGAAAGTCCGAGTAACAGTGAAGCCCCATTTGCTGTACAGACCCTTAGAGCAGCAGCATGGAGTCATTCC TGATCGGGATGCCGAGTTCCGGCTCTTTGATCGCGTTGTGAACGTGAGAGAGAGCTTCTCCGTGCCAGTC GGCCTTCGAGGCACCGTCATTGGAATTAAAGGGGCTAGCAGAGAAGCTGATGTACTATTTGAAGTATTAT TTGATGAGGAATTTCCCGGAGGCTTAACAATAAGGTGCTCACCTGGTAGGGGATACCGACTGCCAACAAG TGCCTTGGTGAACCTTTCTCATGGGAGTCGGTGTGAAACTGGAAACCAGAAGTTGACAGCCATTGTGAAA CCACAACCTTCTGTGAGTCACTGTAGTGCAGCTCCCTCCGGGCACTTGGGAGGCCTCAACCACTCTCCTC AGTCACCTTTTCTTCCTACTCAGGTACCTACAAAAGGTGATGATGAATTTTGCAACATTTGGCAATCTCT ACAGGGAGCTGGAAAAATTCAACACTTGCAGCCGACAGTACAAGAGAAGGGTGCAGTTCTACCTCAAGAA ATAAGTCAAGTAACTGAAGGTCATAAATCTGGCTTTACTGACCACAGTGTCAGACATCAGCAGAGAAAAC ATGACTCGCAACGAAAATTTAAGGAAGAATATAAAAGTCCTAAAGCTGAGTGTCAGAGTCAAAAGTTGTC CAGTAAACAGACTTCTGGAGGGTCTGCCAGATGTAGTATTAAACTATTGAAGAGAAATGAAAGTCCAGGA ACTTCAGAAGCCCAGAAGGTTGTAACTAGTTACCCAAATGCAGTGCATAAGCCTCCCTCTGGGATTGAGA ACTTTTTAGCATCTTTGAATCTCTCTAAGGAGAATGAAGCACAGCTACCTCATCATGGAGAGCCTCCAGA TGAGGCGGATTTGTCCCCGCAGTCCTTTGCTATGAAAGGAACACGGATGCTTAAAGAAATTCTGAAAATT GATAGCCCTGACACAAGAGACAGCAAGAATGATATGAAGAAATCTGATAATGAAGCCACTGTTTCTTCTA GAAGAGATGAGCGTGGAGTGTCAGCACATCCTAAGCCAAGTAAGAAGCTGACATGCCATATGAACAAGCC TCATGGGACTAACGAGTTCCAAAATGTGGCATCGGTGGACAGTGTGTGTTGGCCAGGCCAGATGCCTCCT GTGTCCACACCAGTAACTGAACTTTCTCGAATTTGTTCCCTTGTTGGAATGCCACAACCTGATTTCTCCT TTCTGCGAACAACACAGACAATGACAGTTTGTCAGGTAAAATTGTCTAATGGCTTACTGGTACATGGGCC ACAGTGCCACTCTGAAAGTGAAGCCAAGGAGAGAGCTGCTCTTTTTGCTTTACAGCAACTGGGCTCCTTG GGTGTGAGTTTTCCTTTGCCTCCACCCATATTTACAAATTATCCTCCTGCGGTACCACCTGGAGCCGTCC CTCCTGTTTTTACCCAACCTACTGCTAATATAATGCCTTCATCGTCTCATCTCTTTGGCTCAGTGTCATG GAGACCACCAGTGCCAGTTGCTGGGAATGCCTTCCACTATCCTTCATATCCTGGAACCATGCCCTTGGCT GGAGGAGTGCCAGGCGGAGTGCACAGTCAGTTCATACCTCTGCAGGTTACCAAAAAAAGAGTTGCAAACA GAAAGAATTTTGAGAATAAGGAGGCCCAGAGTTCTCAGGCCACACCACTTCAGACTAACAAGCCAGGGTC TTCTGAAGCCACCAAAATGACTCCACAAGAAAGTCCACCAGCCTCTTCATCATCCTCTCAGGCTGCCCAG CCCGTGTCTTCTCATGTTGAAACTGCCTCCCAAGGCCATGTTGGGTCCCAACCAAGGTCAGCGCCAAGCT CCTCAAAAAGGAAGTCGAGAAAACTGGCCGTCAACTTCAGCGTTTCTAAACCTTCGGAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_011916
Insert Size	5172 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_011916.3, NP_036046.2
RefSeq Size	10278 bp
RefSeq ORF	5172 bp
Locus ID	24127

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