Polr1a (NM_009088) Mouse Untagged Clone

CAT#: MC224866

Polr1a (untagged) - Mouse polymerase (RNA) I polypeptide A (Polr1a), (10ug)



  "NM_009088" in other vectors (2)

CNY 27,550.00


货期*
9周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 194kDa; 3010014K16Rik; mRPA1; RPA194; Rpo1-4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC224866 representing NM_009088
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGGCCTCCAAGCACACACCATGGAGGCGGCTTCAGGGCATTTCCTTCGGAATGTATTCGGCCGAAG
AGCTCAAGAAGTTAAGTGTTAAATCCATTACAAATCCTCGATACGTGGACTACCTGGGAAACCCTTCAGC
AAATGGCCTGTATGATTTGGCTTTGGGTCCTGCAGATTCCAAAGAAGTATGCGCTACCTGCGTGCAGGAC
TTCAACAACTGCTCTGGACACCTGGGCCACATTGATCTCCCGCTCACAGTCTACAACCCTTTCCTCTTTG
ATAAACTCTACCTGCTGCTTCGAGGCTCCTGTTTGAGTTGCCACATGCTGACTTGTCCTCGGGCTGCAAT
TTACCTGTTGATCTCCCAGCTGAGGGTCCTGGAAGTTGGAGCCCTGCAAGCAGTCTACGAGCTTGAGCGA
ATTCTCAGCAGGTTTCTGGAAGAAACTGGAGATCCTTCCGCCTTCGAAATTCAAGAAGAGTTAGAAGAAT
ACACAAGCAAAATTCTGCAGAACAACCTCTTGGGGTCCCAGGGCACACATGTGAAAAATGTGTGCGAGAG
CAGAAGCAAACTTGTCGCTCAGTTCTGGAAGACACACATGGCTGCGAAGCAGTGTCCACACTGCAAGACG
GGACGGTCGGTGGTTCGAAAGGAGCACAATAGTAAACTAATCATCACATACCCAGCCACGGTGCACAAGA
AATCAGACCAGGAGGGCACAGAACTCCCAGAAGGAGTGCCGGAAGCTCCAGGAATTGACAAGGCTCAGAT
GGGGAAACGAGGGTACCTGACCCCAAGCAGCGCCCAAGAACACCTTTTCGCCATTTGGAAGAATGAAGGA
TTCTTTCTGAATTATCTTTTTTCGGGATTGGATGATATTGGCCCAGAGTCCAGTTTCAACCCCAGTATGT
TCTTTCTAGACTTCATAGTCGTACCGCCATCCAGGTACCGTCCCGTCAATCGCCTGGGTGACCAGATGTT
CACTAATGGCCAGACAGTAAACTTGCAAGCTGTCATGAAGGATGCAGTTTTGATCCGGAAACTTCTGGCG
TTGATGGCCCAAGAACAGAAACTGCCCTGTGAGATGACAGAACTCACCATAGACAAGGAAAATGACTCCT
CAGTGGCTATCGACCGATCCTTCCTGGGTTTGCTTCCAGGCCCGTCTCTCACAGATAAACTTTACAACAT
TTGGATTCGCCTTCAGAGCCACGTCAATATTGTGTTTGACAGTGAGATGGACAAATTGATGTTGGAAAAG
TACCCTGGCATAAGACAGATCCTAGAGAAGAAGGAAGGCCTGTTCCGGAAGCACATGATGGGAAAGCGAG
TTGACTACGCAGCCCGCTCGGTCATCTGCCCAGACATGTACATCAACACCAATGAGATCGGAATCCCCAT
GGTATTTGCCACAAAACTGACTTACCCTCAGCCAGTTACCCCCTGGAACGTGCAGGAACTGAGACAGGCA
GTCATCAATGGACCCAATGTGCACCCAGGAGCCTCCATGGTCATCAACGAGGATGGCAGTCGCACAGCCC
TGAGCTCAGTGGATGCTGCGCAGCGGGAAGCTGTGGCCAAACAGCTCCTGACACCAGCCACAGGGGCTCC
GAAGCCTCAGGGGACAAAAGTTGTGTGCCGGCATGTGAAAAATGGGGACATCCTCCTCCTGAACCGACAG
CCTACCTTGCACAGACCCTCCATCCAGGCCCACCGTGCCCGCATTCTGCCTGAAGAAAAAGTCCTACGCC
TTCACTATGCCAACTGCAAGGCCTACAATGCTGACTTTGATGGAGATGAGATGAACGCCCACTTCCCCCA
GAGTGAGTTGGGCCGAGCTGAAGCCTATGTCCTGGCCTGCACTGATCAGCAGTATCTTGTTCCCAAGGAT
GGCCAGCCATTGGCAGGACTGATCCAAGATCACATGGTTTCAGGTGCAAACATGACCATTCGGGGATGCT
TTTTCACCCGGGAACAGTACATGGAACTGGTGTACCGAGGGCTCACAGACAAAGTGGGACGCGTGAAGCT
CTTTCCTCCCGCCATCCTGAAGCCCTTCCCACTGTGGACAGGAAAACAGGTTGTGTCGACCCTCCTCATA
AACATAATTCCAGAGGACTACGCCCCCCTGAACTTATCTGGAAAGGCAAAAATTGGCAGCAAAGCCTGGG
TGAAGGAAAAGCCACGGCCTATCCCTGACTTTGACCCGGACTCCATGTGTGAGTCCCAGGTGATCATCAG
GGAAGGAGAGCTGCTCTGCGGAGTGCTGGACAAGGCACACTATGGGAGCTCTGCCTACGGCCTGGTCCAC
TGCTGCTATGAGATCTATGGGGGTGAGACCAGCGGCAGGGTTCTCACCTGCCTAGCCCGCCTCTTCACTG
CCTACCTCCAGCTCTATAGAGGTTTCACCTTGGGTGTAGAAGACATTTTGGTTAAGCCAAATGCAGATGT
GGTGAGACAGCGTATCATTGAAGAATCTACCCAGTGTGGACCCCAGGCTGTCAAGGCTGCATTGAGCCTG
CCAGAAACTGCATCATGTGATGAAATCCAAGGGAAATGGCAGGATGCCCATCTGAGCAAAGACCAGAGGG
ATTTTAACATGATTGATATGAAGTTCAAGGAGGAAGTGAACCATTACAGCAATGAGATTAACAAGGCATG
TATGCCTCTTGGCTTGCACAGACAGTTCCCCGAAAACAACCTGCAGATGATGGTGCAGTCAGGAGCCAAA
GGGTCAACCGTGAACACGATGCAAATCTCTTGCCTGCTGGGCCAAATTGAATTGGAAGGTCGGAGACCCC
CACTGATGGCGTCTGGCAAGTCACTGCCCTGCTTTGAGCCTTACGAGTTCACCCCCAGGGCTGGTGGCTT
TGTCACTGGAAGGTTTCTTACCGGTATCAGGCCTCCTGAGTTCTTCTTCCACTGCATGGCAGGACGAGAA
GGCCTGGTGGACACTGCTGTCAAAACCAGCCGTTCTGGCTATCTCCAAAGGTGTATCATCAAGCACCTGG
AGGGGCTGGTCATCCAGTACGATCTGACGGTCCGTGACAGCGATGGCAGCGTGGTCCAGTTCCTGTATGG
GGAGGACGGCCTGGACATCCCCAAGACACAGTTCCTACAGCCCAAGCAGTTCCCCTTTCTGGCCGGCAAT
TATGAGGTGATAATGAAATCCAAGCATCTTCATGAAGTTTTATCCAGAGCAGATCCCCAGAAAGTGCTCG
GCCACATCAAAGCCATTAAAAAATGGCATCACAAGCATTCCGGTGCCCTGCTGAGGAAAGGCGCCTTCCT
CAGTTTTTCCCAGAAGATCCAGGCAGCTGTGAAAGCTTTGAACCTAAAGGGCAGTATCCAGAATGGCCGC
AGCCCCGAGACCCAGCAGATGTTACAGATGTGGTATGACTTGGATGAAGAGAGCCGATGGAAATACCAGA
AGAGGGCTGCCCCTTGTCCCGACCCTAGCCTTTCTGTGTGGCGCCCAGACATCTACTTTGCATCTGTGTC
TGAAACATTTGAAAAGAAGATTGATGACTTCAGTCAGGAATGGGCAGCTCAAGCAGAGAGGAGTTACAAG
AAATCAGAGCTTTCCCTCGACAGGCTGAGAACCTTACTGCAGCTGAAGTGGCAGCGCTCACTGTGTGACC
CAGGCGAGGCTGTTGGCCTCCTGGCTGCCCAGAGCATCGGAGAGCCCTCCACACAGATGACACTGAACAC
CTTCCACTTTGCAGGCAGAGGCGAGATGAACGTCACCCTGGGCATTCCAAGATTGAGGGAGATTCTCATG
GTGGCCAGTGCCAACATCAAAACGCCCATGATGAGTGTGCCTGTGTTCGACACCAAGAAAGCATTGAAGA
AAGTGAAGAGTCTAAAGAAGAGGCTCACCAGAGTGTGCCTGGGGGAGGTATTGCAGAAAGTTGACATCCA
GGAGTCCTTCTGTATGGGAGAAAAACGGAATAAGTTCCAGGTGTATGAGCTCCGGTTTCAGTTCCTGCCA
CATGCATATTACCAGCAGGAGAAGTGCCTGAGACCCGAGGACATCTTGCACTTCATGGAAACCAGATTCT
TTAAGCTACTAATGGAAGCTATCAAAAAGAAGAAAAACAAAGCATCAGCCTTCAGAAATGTGAATTCGAG
GAGAGCTACACAGAAGGACCTCAATGACACTGAGGACTCGGGGAGGAGTCAGAGAGAGGAGGAAAGGGAT
GAGGAAGAGGAGGGGAACATTGTGGATGCTGAAGCTGAGGAGGGCGATGCAGACGCTTCTGACACCAAAC
GCAAGGAGAAGCAAGAAGAAGAGGTTGACTATGAGAGTGAGGAAGAAGGAGAGGAGGAAGAGGAGGAGGA
GGTACAAGAAGAAGGGAACATCAAAGGCGACGGTGTCCACCAGGGCCATGAGCCAGATGAGGAAGAACAT
TTAGGCCTCGAGGAGGAGGAGTCATCCCAGAAGCCTCCCCGCCGCCACTCCAGGCCTCAGGGAGCTGAGG
CCATAAAACGCCGGATCCAGGCTGTACGGGAGTCCTACTCGTTCATCGAGGACTACCAGTATGACACAGA
GGAGAGCCTGTGGTGTCAGGTGACTGTGAAGCTCCCACTGATGAAGATTAATTTTGACATGAGCTCCTTG
GTTGTGTCCTTGGCCCACAAAGCCATTGTCTATACGACCAAGGGCATCACTCGGTGCCTCCTGAACGAAA
CGACCAACAGTAAGAATGAGAAGGAGCTTGTACTGAACACGGAGGGAATCAACCTTCCCGAGCTGTTCAA
GTACTCCGAGATACTGGACCTGCGCCGCCTCTACTCCAATGACATCCACGCCATGGCCAATACTTATGGC
ATCGAGGCTGCGTTGCGGGTGATAGAGAAGGAGATTAAAGATGTGTTTGCTGTGTATGGCATTGCAGTTG
ATCCTCGCCATCTCTCCCTGGTGGCTGATTACATGTGTTTTGAGGGGGTTTATAAGCCACTCAATCGCTT
TGGGATCCAGTCAAGCTCCTCCCCTTTGCAGCAGATGACATTTGAAACCAGCTTCCAGTTTCTGAAACAG
GCCACCATGATGGGATCCCATGATGAACTCAAGTCACCTTCCGCCTGCCTCGTGGTGGGAAAGGTTGTCA
AAGGCGGGACAGGCCTGTTTGAGCTCAAGCAGCCCCTAAGATAG


ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
TTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-NotI     
ACCN NM_009088
Insert Size 5154 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_009088.3, NP_033114.3
RefSeq Size 6145 bp
RefSeq ORF 5154 bp
Locus ID 20019
UniProt ID O35134
Gene Summary DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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