Trpm3 (NM_001035243) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	6330504P12Rik; 9330180E14; AU018608; B930001P07Rik; LTRPC3; MLSN2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224840 representing NM_001035243 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAAGAAGTGGAGGGATGCGGGCGAGCTGGAGAGAGGTTGCTCTGACCGCGAGGACAGCGCAGAGA GCCGCAGGCGCAGCCGCAGCGCCAGCCGGGGCAGGTTTGCCGAGTCGTGGAAAAGGTTAAGTTCCAAGCA GGGGTCCACCAAGCGCTCGGGACTGCCGGCACAGCAGACACCGGCTCAGAAATCCTGGATTGAAAGAGCA TTTTATAAAAGAGAATGTGTTCACATCATACCGAGCACCAAAGACCCCCATAGGTGTTGCTGTGGACGCC TCATAGGCCAGCATGTTGGACTCACTCCCAGTATCTCTGTGCTTCAGAATGAGAAAAATGAGAGTCGCCT CTCCCGAAATGACATCCAGTCTGAGAAGTGGTCTATCAGCAAACACACTCAGCTCAGCCCAACAGACGCT TTTGGGACCATTGAGTTCCAAGGAGGTGGCCATTCCAACAAAGCTATGTATGTCCGAGTATCTTTTGATA CGAAACCTGATCTCTTGCTACACCTGATGACCAAGGAATGGCAACTGGAGCTTCCCAAACTTCTCATCTC CGTGCACGGAGGGCTGCAGAACTTTGAACTCCAGCCCAAACTCAAGCAAGTCTTCGGGAAGGGGCTCATC AAAGCAGCCATGACAACTGGAGCCTGGATCTTCACTGGAGGGGTCAACACAGGTGTCATTCGTCATGTTG GAGATGCCTTGAAAGACCATGCATCTAAGTCTCGAGGGAAGATATGTACCATAGGTATCGCCCCCTGGGG AATTGTGGAAAACCAGGAAGACCTGATTGGAAGAGATGTAGTCCGACCATACCAGACCATGTCCAATCCT ATGAGCAAGCTCACCGTTCTCAATAGCATGCACTCCCACTTCATCCTGGCTGACAATGGGACCACGGGGA AATACGGAGCAGAGGTGAAACTCCGCAGACAACTGGAAAAGCACATTTCACTCCAGAAGATAAACACAAG AATCGGTCAAGGGGTTCCAGTGGTGGCTCTCATCGTGGAAGGAGGACCCAATGTGATCTCAATTGTTTTG GAATACCTTCGAGACACCCCTCCTGTACCAGTCGTGGTCTGTGATGGGAGTGGACGGGCATCCGACATCC TGGCATTTGGGCATAAATATTCAGAAGAAGGCGGACTTATCAATGAATCTTTGAGGGACCAGCTGTTGGT GACAATACAGAAGACCTTCACGTACACTCGAACCCAAGCTCAGCACCTGTTCATCATCCTCATGGAATGC ATGAAGAAGAAGGAACTGATCACAGTGTTTCGAATGGGGTCAGAAGGCCACCAGGACATTGATTTAGCTA TCCTGACAGCATTACTCAAAGGTGCCAATGCATCCGCCCCAGACCAACTGAGCTTAGCTTTAGCCTGGAA CAGAGTTGACATCGCTCGCAGCCAGATCTTTATTTACGGGCAGCAGTGGCCGGTAGGGTCCCTGGAGCAA GCCATGCTGGATGCCCTAGTCCTGGACAGAGTGGATTTTGTGAAATTACTCATAGAAAATGGAGTAAGCA TGCACCGTTTTCTCACCATCTCCAGACTAGAGGAACTGTACAATACGAGACATGGGCCCTCAAATACATT GTACCACTTGGTCAGGGATGTCAAAAAGGGAAACCTGCCCCCGGACTACAGAATCAGCCTGATAGACATT GGCCTGGTGATCGAGTACCTGATGGGCGGGGCTTACCGCTGCAACTACACGCGCAAGCGCTTTCGGACCC TCTACCACAATCTCTTTGGCCCCAAAAGGGATGATATTCCCTTGAGGAGAGGACGGAAGACAACTAAGAA GCGTGAGGAAGAGGTGGACATCGACTTGGATGATCCTGAGATCAACCACTTTCCATTCCCCTTCCATGAG CTGATGGTGTGGGCCGTCCTCATGAAGAGACAAAAGATGGCCCTGTTCTTCTGGCAACACGGAGAGGAGG CCATGGCCAAAGCCCTGGTGGCCTGCAAGCTCTGCAAGGCCATGGCTCACGAGGCTTCTGAGAATGACAT GGTCGATGACATTTCCCAGGAGCTGAACCACAACTCCAGGGACTTTGGCCAGCTGGCTGTGGAGCTCCTG GACCAGTCCTACAAACAGGATGAGCAGCTGGCCATGAAGCTGCTGACGTATGAGCTGAAGAACTGGAGTA ATGCCACATGCCTGCAGCTGGCTGTGGCTGCCAAGCACCGTGACTTCATTGCACACACGTGCAGCCAGAT GTTACTTACTGACATGTGGATGGGACGGCTCCGGATGAGAAAGAACTCAGGCCTCAAGGTAATTCTGGGA ATTCTACTTCCTCCTTCAATTCTCAGCTTGGAGTTCAAGAACAAAGATGACATGCCCTATATGACTCAGG CCCAGGAGATTCATCTCCAAGAGAAGGAGCCGGAGGAGCCAGAGAAGCCCACAAAGGAAAAAGATGAAGA GGACATGGAGCTAACAGCAATGTTGGGGCGAAGCAATGGGGAATCATCCAGAAAGAAAGATGAAGAAGAA GTTCAAAGCAGGCACCGGCTAATCCCCGTGGGCCGAAAAATCTATGAGTTCTACAACGCACCCATCGTGA AGTTCTGGTTCTACACTCTGGCGTATATTGGATACCTGATGCTCTTCAACTACATCGTGTTAGTGAAGAT GGAGCGCTGGCCTTCCACTCAGGAATGGATTGTCATCTCCTACATTTTCACTCTGGGAATAGAGAAGATG AGAGAGATCCTGATGTCGGAGCCGGGCAAGCTGCTGCAGAAGGTGAAGGTGTGGCTTCAGGAGTACTGGA ACGTCACAGACCTCATCGCCATCCTTCTCTTCTCGGTGGGAATGATCCTTCGTCTTCAAGATCAGCCCTT CAGGAGTGACGGGAGGGTCATCTACTGTGTGAACATCATTTATTGGTATATCCGTTTGCTGGACATCTTC GGCGTGAACAAGTATCTGGGCCCATATGTGATGATGATTGGGAAAATGATGATAGACATGATGTACTTTG TCATCATTATGCTGGTGGTGCTGATGAGCTTTGGGGTCGCCAGGCAAGCCATTCTCTTTCCCAATGAGGA GCCATCTTGGAAACTGGCCAAGAATATCTTCTACATGCCTTATTGGATGATTTATGGGGAAGTGTTTGCT GACCAGATAGACCCTCCCTGTGGACAGAATGAGACCCGAGAGGATGGCAAGACAATCCAGCTGCCCCCAT GCAAGACAGGAGCATGGATTGTCCCGGCCATCATGGCCTGCTACCTCTTGGTGGCGAACATCCTTCTGGT CAACCTCCTTATTGCCGTCTTCAACAATACATTTTTTGAGGTCAAGTCGATATCCAACCAAGTATGGAAA TTTCAGAGGTACCAGCTCATCATGACTTTCCACGAGAGGCCGGTTCTGCCCCCACCGCTCATCATCTTCA GTCACATGACCATGATCTTCCAGCATGTGTGCTGCCGGTGGAGGAAGCATGAGAGTGACCAGGACGAAAG GGACTACGGCCTGAAACTCTTCATAACTGACGATGAGCTCAAGAAAGTACACGATTTTGAAGAGCAGTGC ATAGAGGAATATTTCCGAGAGAAGGATGATCGCTTCAATTCGTCCAACGATGAGAGGATACGTGTTACAT CAGAAAGGGTGGAGAACATGTCCATGAGGCTGGAGGAAGTTAATGAGAGAGAACATTCCATGAAGGCTTC ACTCCAGACCGTGGACATCCGGCTAGCACAGCTAGAGGACCTCATCGGGCGCATGGCCACCGCCCTGGAG CGCCTGACTGGTCTGGAGCGGGCAGAGTCTAACAAAATCCGCTCAAGGACCTCCTCAGACTGCACAGATG CAGCCTACATCGTCCGCCAGAGCAGCTTCAACAGCCAGGAAGGGAACACCTTCAAACTTCAAGAGAGTAT AGACCCTGCAGGTGAGGAGACCATATCCCCAACTTCTCCAACCTTAATGCCCCGTATGCGAAGCCATTCT TTCTATTCAGTCAATGTGAAAGACAAAGGTGGGATAGAAAAGTTGGAAAGCATTTTCAAAGAAAGGTCCC TGAGCTTACACCGAGCTACTAGCTCCCACTCAGTAGCCAAAGAACCCAAAGCTCCTGCAGCCCCTGCAAA CACCTTGGCCATTGTTCCTGACTCCAGAAGACCATCATCTTGCATAGACATCTATGTCTCTGCCATGGAC GAGCTCCACTGTGATATAGAGCCTCTGGATAATTCCATGAACATCCTTGGGCTGGGTGAGCCAAGCTTTT CAGCTCTAGCACCTTCCACAACCCCGTCAAGTAGTGCCTATGCAACGCTCGCACCTACAGACCGACCTCC CAGTAGGAGCATTGATTTTGAAGACCTCACCTCCATGGACACTAGATCTTTTTCTTCAGACTATACACAC CTCCCAGAATGCCAAAACCCCTGGGACACAGACCCTCCAACGTACCATACCATCGAGCGTTCCAAGAGTA GCCGCTACCTAGCCACCACGCCCTTTCTTCTGGAAGAGGCTCCCATTGTAAAATCCCATAGCTTTATGTT TTCTCCTTCAAGGAGCTACTACGCCAACTTTGGGGTGCCCGTGAAAACGGCAGAATACACAAGTATTACA GACTGTATCGACACAAGATGTGTCAATGCCCCCCAAGCAATAGCTGACCGAGCCACCTTCCCTGGAGGTC TCGGAGACAAAGTGGAAGATTTATCTTGTTGCCACCCTGAGCGAGAAGCAGAGCTGAGCCATCCTAGCTC TGACAGTGAGGAAAATGAGGCCAGAGGCCAGAGAGCTGCCAATCCGATATCCTCTCAGGAGGCTGAAAAT GCAGACAGAACCCTATCCAACAACATCACAGTTCCCAAGATAGAGCGCGCCAACAGCTACTCAGCAGAGG AGCCAAACGTGCCATATGCACATACCAGAAAGAGCTTCTCCATCAGTGACAAGCTTGATAGACAGAGGAA CACGGCGAGCCTCCGAAATCCCTTCCAGAGAAGTAAGTCCTCCAAGCCGGAGGGCCGAGGGGACAGCCTA TCCATGAGGAGACTGTCTAGAACATCGGCCTTTCATAGCTTTGAAAGCAAGCACAACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001035243
Insert Size	5100 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001035243.2, NP_001030320.1
RefSeq Size	6676 bp
RefSeq ORF	5100 bp
Locus ID	226025

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