Cul7 (NM_025611) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2510004L20Rik; AA409809; C230011P08Rik; p185; p193
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224832 representing NM_025611 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTAGGGGAGCTACGATACAGGGAATTCAGGGTGCCCCTGGGGCCTGGCTTGCACGCGTATCCGGATG AATTGATCCGCCAACGGGTTGGCCATAATGGGCACCCCGAGTATCAGATCCGCTGGCTCATCCTCAGGCG CGGGGATGATGGGGACCGGGACTCTACAGTGGACTGCAAGGCTGAGCATATCCTGTTATGGATGTCTGAC GATGAGATCTATGCCAACTGCCACAAGATGCTGGGCGAGAATGGCCAAGTCATCGCACCTTCCCGGGAGT CCACTGAGGCAGGGGCCCTCGACAAGTCTGTGCTGGGGGAGATGGAAACAGATGTGAAGTCCTTGATTCA GAGGGCCCTTCGGCAGCTGGAGGAGTGCGTGGGCACCGTGCCTCCTGCCCCTCTCCTTCACACGGTCCAT GTACTCAGTGCCTATGCCAGCATCGAGCCCCTCACTGGCATCTTCAAAGACCGCAGGGTTGTGAACTTGC TCATGCACATGTTGAGCAGTCCTGATTATCAGATCCGCTGGAGCGCAGGCCGGATGATCCAAGCTCTGTC CTCCCACGATGCTGGGACCCGGACCCAGATCCTTCTGTCATTGAGCCAACAAGAGGCCATTGAAAAGCAC CTGGATTTTGATAGCCGCTGCGCTCTGCTTGCACTGTTCGCCCAGGCTACTCTCACGGAACACCCGATGT CTTTCGAGGGCGTTCAGCTGCCACAGGTCCCAGGACGGCTGCTCTTCTCCCTGGTAAAACGCTACCTGCA CGTCACCTTCCTCCTGGATCGGCTGAACGGCGATGCAGGGGATCAAGGAGCCCAGAACAACTTTATTCCT GAGGAGTTGAATGTAGGGAGGGGCCGGCTGGAACTGGAATTCAGTATGGCCATGGGCACTCTGATCTCTG AGCTGGTGCAGGCCATGCGCTGGGACGGGGCCTCAAGCAGACCAGAGAGTTCTTCCTCCTCCACCTTCCA GCCTCGGCCAGCACAGTTCCGCCCCTACACCCAGCGTTTCAGGAGGTCGAGGCGGTTTCGCCCCCGTGCC TCGTTTGCCAGTTTTAATACCTATGCCTTGTATGTGCGGGACACGCTGCGGCCCGGGATGCGGGTACGGA TGCTGGAGAATTACGAGGAGATCGCTGCTGGGGATGAGGGCCAGTTCCGACAGAGCAATGATGGCGTTCC CCCAGCGCAGGTGTTGTGGGATTCAACAGGCCATACCTACTGGGTGCACTGGCACATGCTGGAGATCTTG GGCTTTGAGGAAGACATCGAGGATGTGATTGATATTGAAGAGTTACAGGAGCTAGGGGCCAATGGAGCAC TGAGCATCGTCCCGCCGTCCCAGCGCTGGAAGCCCATAACTCAGCTCTTTGCCGAGCCTTACGTGGTACC CGAGGAGGAAGACAGGGAAGAGAGCGAGAACTTGACCCAGGCTGAGTGGTGGGAGCTCCTCTTCTTCATC CGGCAGTTGAGTGAGGCAGAGCGCCTTCACATCGTGGATCTCCTGCAAGACCACCTGGAAGAGGAGCGCG TTCTGGACTACGATATGCTGCCTGAGCTGACCGTGCCCGTTGACTTGGCCCAGGATCTGCTGTTGTCTCT GCCTCAGCAACTTGAGGACAGTGCTCTGAGGGACCTGTTCAGCTGCAGTGTCTACAGGAAGTATGGGCCC GAAGTCCTGGTAGGGCATCTAAGCTACCCATTTGTGCCAGGTGCCCAGCCAAATTTATTCGGAGCCAATG AAGAGTCTGAAGCCAAAGATCCCCCACTTCAGAGTGCCAGCCCTGCCCTGCAGCGCCTGGTGGAGAGCTT GGGCCCCGAAGGGGAGGTCCTTGTGGAACTGGAACAAGCCCTCGGCTCCGAGGCTCCCCAGGAAACTGAG GTCAAGTCCTGCTTGTTGCAGCTCCAGGAGCAGCCCCAGCCCTTCCTCGCTCTGATGCGGAGCCTGGACA CTTCCGCCAGCAACAAGACCCTGCACCTCACTGTGCTCAGAATCTTAATGCAGCTGGTGAACTTCCCAGA GGCGCTGTTGCTACCCTGGCACGAGGCCATGGATGCCTGCGTGACCTGCCTTCGGTCCCCCAATACTGAC CGAGAGGTGCTCCAGGAACTAATCTTTTTCCTGCACCGCCTGACCACCACAAGCCGGGACTATGCGGTGA TACTAAACCAGCTAGGAGCCCGGGACGCCATCTCCAAAGTCCTGGAAAAGCACCGAGGGAAACTGGAGTT GGCTCAGGAGCTGCGGGATATGGTGTCCAAGTGTGAGAAGCATGCCCACCTCTACCGGAAACTCACCACC AACATCCTGGGCGGTTGCATCCAGATGGTCCTGGGTCAGATTGAAGACCACAGACGAACCCACCGGCCCA TCCAAATCCCATTCTTTGATGTGTTTCTCAGATATCTGTGCCAGGGCTCCAGTGAGGAAATGAAGAAAAA CAGGTACTGGGAGAAGGTGGAGGTGTCCTCCAACCCACAGCGGGCCAGCAGGCTGACGGACCGCAACCCC AAGACCTACTGGGAGTCCAGTGGCAGGGCCGGCTCCCACTTCATCACCTTACACATGCGCCCAGGTGTCA TCATCAGGCAGCTGACTCTACTGGTGGCTGGCGAGGACTCAAGCTACATGCCAGCCTGGGTGGTGGTATG CGGGGGCAACAGCATCAAGTCCGTTAATAAAGAACTCAACACGGTAAACGTGATGCCCTCTGCCAGCCGG GTGACCCTCCTGGAGAACCTGACCCGCTTCTGGCCCATCATCCAAATCAGAATAAAGCGCTGCCAGCAGG GTGGCATTAACACGCGCATCCGGGGCCTAGAGGTGCTGGGCCCCAAGCCCACCTTCTGGCCAGTGTTCCG AGAGCAACTGTGCCGGCACACGCGCCTCTTCTACATGGTTCGGGCCCAGGCATGGAGTCAGGACATAGCA GAGGACCGCCGGAGCCTTCTGCACCTGAGTTCTAGGCTAAATGGGGCTCTGCGCCATGAACAGAATTTTG CAGAGCGCTTCCTTCCTGATATGGAGGCCGCCCAAGCACTGAGCAAGACCTGCTGGGAGGCGCTGGTCAG CCCCCTGGTGCAGAACATTACATCTCCCGATGAGGACAGCACCAGCTCCTTGGGCTGGCTGCTGGATCAG TACTTGGGATGCAGGGAGGCTGCCTACAATCCCCAGAGCAGGGCTGCTGCTTTCTCCTCCCGGGTTCGCC GCCTTACCCACCTCCTGGTCCATGTGGAGCCCCGTGAGGCAGCACCTCCGGTGGTGGCCATCCCTCGATC CAAGGGCAGGAACAGAATCCATGACTGGAGCTACTTGATCACCCGGGGCCTTCCAAGTAGCATCATGAAG AACCTGACCCGCTGTTGGCGGTCTGTGGTGGAGGAGCAGATGAACAAGTTTCTGACTGCGTCCTGGAAAG ACGATGATTTCGTACCCCGCTATTGCGAGCGCTATTACGTCCTGCAGAAGTCCAGCTCAGAGCTGTTTGG GCCACGAGCTGCCTTCTTGCTGGCAATGCGGAATGGCTGTGCTGATGCTGTGCTGAGGCTCCCTTTCCTC AGGGCCGCCCATGTGAGCGAGCAGTTTGCTCGGCACATTGACCAGAGGATCCAAGGCAGTAGGATGGGTG GAGCCCGGGGAATGGAGATGCTGGCACAGTTGCAGCGATGCCTGGAGTCTGTCCTGATTTTCTCTCCCCT GGAGATAGCCACCACCTTTGAGCATTACTACCAGCACTACATGGCTGACCGTCTCCTGAGCGTAGGCTCC AGCTGGCTGGAGGGGGCCGTACTGGAGCAGATCGGTCCCTGCTTCCCCAGCCGTCTTCCCCAGCAGATGC TACAGAGCCTGAACGTCTCAGAGGAGTTGCAGCGTCAGTTCCACGTTTACCAGCTGCAGCAGCTTGATCA GGAGCTCCTGAAGCTGGAAGACACGGAAAAGAAGATACAGGTGGCCCATGAGGACAGTGGCAGAGAGGAC AAGAGCAAGAAGGAAGAAGCCATTGGAGAAGCCGCGGCTGTGGCTATGGCAGAGGAGGAGGATCAAGGGA AGAAGGAGGAGGGAGAGGAGGAAGGGGAGGGAGAGGATGAGGAGGAAGAGCGCTATTATAAAGGAACAAT GCCAGAAGTGTGTGTACTTGTCGTGACGCCACGCTTCTGGCCTGTCGCCTCCGTCTGCCAAATGCTCAAC CCGGCAACGTGCCTGCCCGCATACCTGCGGGGGACCATAAACCACTACACCAACTTTTACAGCAAGAGTC AGAGCCGCTCCAGCTTAGAGAAAGAGCCACAGAGGCGGCTGCAGTGGACCTGGCAGGGCCGGGCAGAAGT GCAGTTCGGGGGTCAGATTCTGCATGTGTCCACAGTACAGATGTGGCTGCTGCTGCATCTCAACAACCAA AAGGAGGTGTCTGTCGAGAGCCTGCAGGCTATCTCGGAGCTCCCTCCAGATGTGCTTCACAGGGCCATCG GGCCTCTCACCTCATCAAGAGGTCCCTTGGACCTGCAGGAGCAGAAGAACGTACCAGGAGGGGTGCTCAA GATTCGAGATGACAGTGAGGAACCCAGGCCGAGGAGGGGCAACGTGTGGCTGATCCCACCTCAGACATAC CTACAAGCTGAGGCCGAAGAGGGCCGGAACATGGAGAAGAGAAGGAATCTTCTGAATTGCCTTGTTGTCC GAATCCTCAAGGCTCACGGGGATGAAGGCTTGCATGTTGACCGGCTCGTCTATCTGGTGCTAGAAGCGTG GGAGAAAGGCCCGTGTCCTGCTAGGGGTCTGGTCAGCAGCCTCGGCAGGGGAGCAACCTGCAGGAGCTCT GATGTCCTCTCCTGCATCCTGCACCTCCTGGTCAAGGGCACGCTGAGACGCCATGACGACCGGCCGCAGG TGCTGTACTATGCAGTTCCTGTAACTGTGATGGAGCCCCACATGGAGTCCCTGAACCCTGGCTCGGCAGG CCCCAATCCACCCCTCACCTTCCACACCCTGCAGATTCGATCCCGGGGTGTGCCTTACGCCTCCTGCACT GATAACCACACCTTCTCCACTTTCCGGTAG ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA TTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-NotI
ACCN	NM_025611
Insert Size	5070 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_025611.5, NP_079887.3
RefSeq Size	5546 bp
RefSeq ORF	5070 bp
Locus ID	66515
UniProt ID	Q8VE73
Gene Summary	Core component of the 3M and Cul7-RING(FBXW8) complexes, which mediates the ubiquitination of target proteins. Core component of the 3M complex, a complex required to regulate microtubule dynamics and genome integrity. It is unclear how the 3M complex regulates microtubules, it could act by controlling the level of a microtubule stabilizer. Interaction with CUL9 is required to inhibit CUL9 activity and ubiquitination of BIRC5. Core component of a Cul7-RING ubiquitin-protein ligase with FBXW8, which mediates ubiquitination and consequent degradation of target proteins such as GORASP1, IRS1 and MAP4K1/HPK1. Ubiquitination of GORASP1 regulates Golgi morphogenesis and dendrite patterning in brain. Mediates ubiquitination and degradation of IRS1 in a mTOR-dependent manner: the Cul7-RING(FBXW8) complex recognizes and binds IRS1 previously phosphorylated by S6 kinase (RPS6KB1 or RPS6KB2). The Cul7-RING(FBXW8) complex also mediates ubiquitination of MAP4K1/HPK1: recognizes and binds autophosphorylated MAP4K1/HPK1, leading to its degradation, thereby affecting cell proliferation and differentiation. Acts as a regulator in trophoblast cell epithelial-mesenchymal transition and placental development. Does not promote polyubiquitination and proteasomal degradation of p53/TP53. While the Cul7-RING(FBXW8) and the 3M complexes are associated and involved in common processes, CUL7 and the Cul7-RING(FBXW8) complex may be have additional functions (By similarity). Probably plays a role in the degradation of proteins involved in endothelial proliferation and/or differentiation.[UniProtKB/Swiss-Prot Function]

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