Col4a3 (NM_007734) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	alpha3(IV); [a]3(IV)
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224807 representing NM_007734 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACTCCAAGACTGCTCCAAGGTTCCTGGTGTTCCTGCTGCTTACCCTGCTGCTACTCCTGGCTGCCT CTCCTGTGGCTAGCAAGGGCTGTGTCTGCAAAGGCAAAGGACAATGCCTCTGCGCGGGGACCAAAGGGGA GAAGGGGGAGAAAGGGGTTCCTGGTTCCCCTGGATTTCCTGGCCAGAAAGGATTTCCAGGTCCTGAAGGC TTGCCTGGACCACAGGGACCCAAGGGCTCCCCAGGACTTCCGGGACTCACTGGCCCCAAAGGCATCAGGG GAATAACTGGATTACCAGGGTTTGCAGGTCCTCCTGGACTTCCAGGCCTCCCAGGCCACCCTGGGCCTCG TGGGCTGGCTGGTTTACCAGGATGCAACGGATCTAAGGGTGAACAAGGATTCCCGGGCTTTCCCGGCACA CCAGGCTATGCAGGGCTCCCAGGTCCTGATGGCTTGAAAGGACAAAAGGGTGAGCCTGCTCAAGGAGAAG ACAGGGGATTCAATGGAAAAGGTGACCCTGGGCCTCCAGGGGTTCCAGGCTTCCAGGGTTTTCCGGGACT CCCAGGTTTTCCAGGGCCTGCCGGTCCACCAGGACCTCCGGGATTTTTTGGTTTACCAGGAGCAATGGGA CCCAGAGGACCCAAAGGCCACATGGGCGATAGCGTGATAGGACAAAAAGGAGAAAGGGGTATGAAAGGAT TAACAGGACCTCCTGGGCCACCAGGAACAGTGATTTTTACACTCACCCAGCCATACAACAAATCGGACTT CAAGGGAGAGAAAGGAGATGAGGGAGAGAGGGGTGAACCCGGACCTCCTGGACCCTCGGGGCCACCTGGA GACTCCTATGGATCAGAAAAGGGTGCGCCTGGAGAGCCTGGTCCTCGGGGCAAACCTGGAAAAGATGGTG CCCCTGGCTTCCCTGGCACTGAGGGAGCCAAAGGCAACAGGGGCTTTCCTGGGTTGAGGGGTGAAGCTGG CATTAAGGGAAGGAAAGGAGACATTGGCCCCCCAGGATTTCCTGGTCCAACAGAATATTATGACGCATAC CTGGAAAAGGGAGAGAGAGGAATGCCAGGCCTTCCTGGGCCCAAAGGAGCCCGTGGCCCACAGGGTCCAA GTGGTCCCCCTGGAGTCCCTGGAAGTCCTGGACTGTCAAGGCCTGGCCTCAGAGGACCCATTGGATGGCC AGGCTTGAAAGGGAGTAAAGGAGAGAGAGGACCCCCTGGAAAAGACACTGTGGGCCCTCCTGGACCCCTG GGATGTCCTGGCTCACCAGGTCCACCAGGCCCTCCAGGACCTCCAGGATGTCCAGGTGACATTGTTTTTA AATGTTCCCCTGGTGAGCATGGAATGCCAGGTGACACAGGACCTCCAGGAGTTCCAGGACTTGATGGACC CAAAGGAGAACCAGGCAGTCCATGCACCGAGTGTCACTGCTTCCCAGGGCCCCCTGGAGTCCCAGGATTT CCAGGATTAGATGGTATCAAAGGAATCCCAGGAGGACGAGGTGTGCCTGGTCTGAAGGGAAACCCAGGGT CCCCAGGAAGTGCAGGTCTCCCAGGATTTGCAGGATTCCCAGGTGACCAAGGGCATCCAGGACTTAAAGG GGACAAAGGGGATACACCTCTACCCTGGGGGCAAGTGGGTAATCCAGGTGATCCTGGACTCAGAGGCCTG CCCGGGAGAAAGGGCTTCGATGGAACTCCTGGAGGTCCAGGAGCGAAAGGACCACCAGGACCTCAGGGCG AACCGGCCCTGAGTGGAAGGAAAGGGGACCAAGGACCTCCAGGACCTCCTGGATTCCCTGGACCCCCAGG ACCTGCAGGACCAGCTGGACCACCAGGCTATGGACCTCAAGGAGAGCCAGGTCCAAAGGGAGCCCAAGGA GTCCCCGGCGTCCTGGGACCACCTGGAGAAGCCGGTCTTAAAGGAGAACCCAGTACATCAACTCCAGATC TAGGTCCCCCAGGCCCTCCAGGGCCCCCTGGTCAGGCTGGCCCCAGAGGTCTACCTGGTTTGCCTGGACC GGTGGGAAAATGTGATCCTGGTCTTCCTGGACCTGATGGTGAGCCAGGAATTCCAGAAGCTGGATGCCCA GGGCCTCCAGGACCTAAGGGAAATCAAGGTTTCCCAGGGACAAAAGGATCACCTGGTTGTCCTGGGGAAA TGGGAAAGCCTGGCCGTCCTGGAGAACCAGGCATCCCAGGAGCCAAGGGAGAACCATCCGTAGGCAGGCC TGGGAAACCAGGGAAACCAGGCTTTCCAGGAGAAAGAGGCAATGCTGGGGAAAATGGAGATATTGGACTC CCTGGGCTTCCAGGCCTCCCTGGAACTCCAGGAAGAGGCGGGCTTGACGGGCCTCCAGGAGACCCAGGAC AGCCTGGATCACCTGGAGCAAAAGGATCCCCAGGAAGGTGCATACCAGGACCCAGGGGCACCCAAGGACT TCCCGGCTTGAATGGATTGAAAGGGCAACCAGGTCGAAGAGGTGATACCGGGCCAAAGGGAGACCCTGGC ATCCCAGGCATGGACAGGTCTGGGGTCCCCGGAGATCCTGGACCACCAGGAACACCAGGTTGTCCTGGTG AGATGGGACCACCTGGACAAAAAGGATATCCGGGAGCTCCAGGATTCCCAGGACCACCAGGCGAGAAAGG AGAGGTTGGAATGATGGGCTATCCTGGAACCACTGGCCCTCCGGGGCTTCCTGGGAAACCAGGCTCACAG GGGCAGCGAGGTAGCCTCGGAATCCCAGGAATGAAAGGGGAAAAAGGACGCCCAGGAGCCAAAGGCGAAC GAGGAGAGAAAGGAAAACCAGGGCCTTCTCAAACAACACTCTTAAAGGGAGACAAAGGAGAGCCTGGACT TAAAGGATTTGTTGGGAATCCAGGTGAGAAAGGAAACAGAGGCAACCCAGGGTTACCAGGTCCGAAAGGC CTCGAGGGATTGCCTGGGCTACCAGGTCCTCCAGGCCCTAGAGGAGATACGGGAAGCAGAGGAAATCCTG GAAGACCAGGACCACACGGCATGCCAGGAAGCATGGGGATCATGGGGGTGCCAGGCCCTAAAGGGAGAAA AGGAACTTCAGGGCTTCCAGGTCTAGCTGGAAGACCAGGCCTGACAGGGATCCATGGTCCCCAAGGAGAT AAGGGGGAGCCGGGTTATTCAGAAGGTGCAAGACCAGGACCACCAGGACCAAAGGGCGATCCAGGATTGC CAGGTGACAAAGGAAAGAAAGGAGAAAGAGGCGTACCTGGGCCACCTGGACAATCGGGGCCTGCCGGACC TGATGGAGCCCCTGGGAGTCCTGGGAGTCCTGGTCACCCAGGAAAACCAGGTCCTGCTGGTGATTTGGGT CTGAAAGGACAGAAAGGCTTCCCAGGCCCTCCAGGAAGCACTGGCCCTCCAGGCCCTCCAGGACTCCCAG GACTTCCTGGGCCAATGGGTATGAGAGGTGACCAAGGACGTGATGGAATTCCTGGTCCTCCAGGAGAAAA AGGAGAAACAGGCTTGCTGGGGGCCTACCCAGGCCCAAAAGGGAGCCCTGGTGTACCAGGTGCCAAAGGA GACAGGGGAGTTCCAGGCTTGTCTGGCCTTCCCGGCAGGAAGGGGGTTATGGGGGATGTTGGACCCCAAG GACCCCCAGGCACTGCTGGACTCCCAGGGCCACCAGGTCTACCTGGGGCAATTATCCCTGGCCCCAAAGG AGACAGAGGTCTTCCTGGCTTAAGAGGAAATCCAGGTGAGCCAGGTCCCCCTGGACCTCCAGGACCTATC GGAAAAGGCATAAAAGGTGACAAAGGATTTATGGGCCCACCTGGCCCCAAAGGCCTGCCTGGAACTGTAG GGGACATGGGCCCACCAGGTTTCCCGGGAGCACCAGGTACCCCCGGTCTTCCCGGTGTCAGAGGTGATCC AGGATTCCCTGGATTTCCAGGCATAAAAGGAGAAAAGGGTAACCCTGGATTTCTTGGGCCAATTGGACAT CCAGGACCAGTTGGGCCCAAAGGACCACCAGGTCCACGTGGAAAACCTGGCACTCTTAAGGTCATCTCTC TTCCGGGAAGCCCAGGGCCACCTGGTGTACCTGGACAGCCAGGAATGAAAGGAGACCCTGGACCCCTGGG ACTGCCAGGAATCCCAGGACCCTGTGGGCCAAGAGGTAAACCAGGCAAGGATGGGAAACCAGGAACTCCA GGACCAGCTGGTACAAAGGGCAACAAGGGCTTGAAAGGACAGCAAGGCCCACCTGGTCTGGATGGATTGC CAGGCTTAAAGGGAAATCCTGGTGACAGGGGAACGCCGGCCACTGGTACAAGAATGCGAGGCTTCATCTT CACCCGACACAGTCAAACCACGGCCATTCCTTCATGCCCTGAAGGAACACAGCCACTCTATAGTGGGTTT TCTCTTCTTTTTGTACAAGGAAACAAACGTGCACATGGACAAGACCTAGGTACTCTGGGCAGCTGCCTGC AGCGATTCACCACAATGCCGTTCTTATTCTGTAACATCAATAATGTATGTAACTTTGCATCACGAAATGA TTATTCATACTGGCTGTCAACACCAGCTCTGATGCCAATGGACATGGCTCCAATTAGTGGCAGAGCTCTC GAACCCTATATTAGCAGATGCACCGTCTGTGAAGGTCCAGCAATGGCCATAGCTGTTCACAGTCAAACTA CTGCTATCCCTCCGTGTCCCCAGGACTGGGTTTCTCTCTGGAAAGGTTTTTCTTTCATTATGTTCACAAG TGCAGGCTCTGAGGGTGCTGGACAAGCACTTGCCTCGCCTGGCTCCTGCCTGGAAGAATTCCGAGCCAGT CCATTTATAGAATGCCATGGACGAGGGACATGTAACTACTACTCAAACTCCTACAGTTTCTGGCTGGCTT CGCTGAACCCAGAAAGAATGTTCAGAAAACCTATTCCATCAACTGTGAAAGCTGGAGACTTAGAGAAAAT CATAAGCCGCTGTCAGGTGTGCATGAAGAAAAGACATTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_007734
Insert Size	5010 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_007734.2, NP_031760.2
RefSeq Size	8609 bp
RefSeq ORF	5010 bp
Locus ID	12828
UniProt ID	Q9QZS0
Gene Summary	Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.[UniProtKB/Swiss-Prot Function]

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