Zcchc11 (NM_175472) Mouse Untagged Clone

CAT#: MC224784

Zcchc11 (untagged) - Mouse zinc finger, CCHC domain containing 11 (Zcchc11), (10ug)



  "NM_175472" in other vectors (6)

CNY 21,000.00


货期*
9周

规格
    • 10 ug

Product images

经常一起买 (4)
Goat Polyclonal Antibody against Terminal Uridylyltransferase 4 (mouse)
    • 100 ug

CNY 5,371.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 6030404K05Rik; 9230115F04Rik; mKIAA0191; PPAPD3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC224784 representing NM_175472
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGAGCCCAAGACTTCTAAAAACGAAAATCATGAACCAAAGAAGAATATTATTTGTGAAGAAAGCA
AAGCAGTTAAAATTATATCTAATCAAACCTTGAAGCCTAGAAATGATAAGTCAGAAATTGGGACCAGCTC
TCTGAATAGAAATAGTAGTAAAAAAACCAAGCAAAATGATATTTGTATAGAAAAAACAGAAGCTAAATCA
TGTAAAGTAAACGCTGCCAGTGTTCCAGGCCCTAAAGATTTGGGGTTAGTACATCGAGATCAAAGTCATT
GCAAAATGAAAAAGTTACCAAATTCACCCATGAAAGCCCAGAAGGGATCTTCACAGACAAAATTAGAAAA
GACACCTAGTTTACAGACAAAAGCAGAAAAAGTACCAAAGTCTCCAAATTTACCAGTAAAAGCAGAAAAA
GCGCCCTGTACAACAGCAGAAGCAACAACAGAAAAAGCACTTAATTCACAGAGAAAAGAAGAAAACACAC
CCACTTCTCAGATGAAATTACAGAAGACACCCAGATCACCTTTAGAGCCAGAAAATGTGCCCAGTTTACT
ATTGAAAGAAAATGTGAAACAGACAGAATCACAACAGACTGGGAAGAAACTTACAAGCTCCTTTGTTTCT
ATGGATAAAAGGAAGAGTGAAGCTCTACAGGGAGAAAAGTCAGCTCTGGAAAACTCATCACTATCTCAGA
AGCAGCAGACTCAGACAGATAATATTGCTGATTCTGATGACAGTGCTTCAGGGATTGAAGATACAGCAGA
TGATTTGAGTAAAATGAAGAGTGAGGAATCTAACAAAGAAAATTCTTCTGAGATGGACTATTTAGAAAAT
GCCACTGTAATAGATGAATCTGCTTTGACACCTGAGCAGAGGCTAGGCTTGAAACAAGCAGAAGAACGTC
TAGAAAGAGATCACATCTTCCGACTTGAAAAACGGTCACCAGAATATACCAATTGCCGCTATCTATGCAA
ACTTTGCTTAATTCACATTGAAAACATCCAAGGAGCACATAAACATATAAAAGAAAAACGACATAAGAAA
AATATTTTGGAAAAACAAGAAGAGAGTGAACTTCGGTCTCTTCCATCTCCTTCCTCTGCTCACTTGGCTG
CTTTAAGTGTTGCAGTTGTTGAGTTAGCAAAAGAACAAGGAATAACAGATGATGACCTCAGAATCCGCCA
GGACATTGTGGAGGAAATGTCAAAGGTTATAATGACATTTTTACCAGAATGTTCACTGAGGTTGTATGGT
TCATCTCTGACAAAGTTTGCTCTGAAAAGCAGTGATGTTAATATAGATATAAAATTTCCTCCCAAGATGA
ATCATCCAGATCTTCTGATACAAGTGCTTGGAATTCTGAAAAAAAGTGCATTATATATAGATGTGGAATC
TGATTTTCATGCTAAAGTTCCTGTTGTGGTGTGCAAAGATCGGAAAAGTGCTTTACTGTGTAGAGTAAGT
GCAGGAAATGATATGGCGTGTCTCACAACTGACTTACTTGCTGCTCTTGGCAAAGTGGAACCCGTCTTCA
CTCCCTTAGTGCTAGCTTTTCGCTATTGGGCTAAGCTGTGCTATATTGACTCCCAAACTGATGGTGGAAT
CCCTTCTTACTGCTTTGCTTTGATGGTGATGTTTTTTCTTCAACAAAGAAAACCCCCTCTTCTTCCTTGC
TTACTTGGAAGTTGGATTGAAGGCTTTGACCCGAAAAGAATGGATGACTTTCAGCTGAAGGGCATAGTAG
AAGAGAAGTTTGTGAAGTGGGAATATAATTCAAGTAGTGCAACTGAGAAAAACTTAATTGCTGATGAAAA
CAAAGCTAAGGCAGACGAACCAAAAGATGATACCAAGAAGACAGAAACAGACAACCAAAGTAATGCTGCG
AAGGCAAAACATGGCAAATCTCCTTTAACATTGGAAGCACCAAATCAGGTACCCTTGGGACAGTTGTGGT
TGGAGCTGCTAAAATTTTACACACTGGATTTTGCTTTGGAGGAATATGTCATATGTGTACGGATACAAGA
TATTCTAACTAGAGAGAACAAAAACTGGCCTAAAAGGCGGATAGCCATTGAAGATCCATTTTCAGTCAAG
AGGAATGTTGCCCGGAGCTTAAACAGCCAGCTTGTATACGAATATGTTGTGGAAAGATTCAGAGCAGCTT
ATCGATATTTTGCCTGTCCTCAAAAGAAGGGTGGAAATAAATCTACAATGGATCCTAAGAAGAAAGAGAA
GGGGAAGCTAAGCAGTAAGAAACCAGTGAAGTCTGATTGTTCGGCAACCAATTGTTGCATTCTTGGGGAA
AGTGCAGAAAAAATACATATGGAAAGAGGCCAACCTGCTAAACATGATGAAACGGAGTTTACATCACAGA
GATGTATTGTTGATAATGACAGTTTATTGGTAAATGAACTAGGTTTGGCTAACCATGGACAAGATTCTTC
ATCTCTTTCTACTGCCAGTGGAGGCAGTGACTTAAAGCAAAAATCAGCTGAGAAACAAGGTGATTTAACA
CCTTCAGAAACTTCCTTAAAGAAGGAACTCAGCCAGTGTATTTGCATTGGGACCCCTGATGGAGCTGAAT
CTGCTGGAACAGACTGCAGATCAAACTTAGAAATGGACAGTTCACATCAGATTGTGTGCAACAATGTATC
TGCTACCTCTTGCAACTGCAAAGCTACAGAAGTTACTTCTGACCTTGTTGATGAGGATAACCTCCCCTCC
CAGGAATTATATTATGTGTTTGATAAGTTCATTTTAACCTCTGGCAAGCCGCCAACAATAGTATGCAGCA
TCTGCAAAAAGGATGGCCATTCAAAAAATGATTGTCCAGAGGATTTCAGGAAAATTGATCTAAAACCTCT
ACCACCAATGACAAACCGATTTCGAGAAATACTTGATTTAGTATGTAAAAGATGTTTTGATGAATTATCA
CCACCTTGCTCTGAACAACACAACAGGGAACAAATTTTAATTGGCTTGGAAAAGTTTATTCAAAAAGAAT
ATGATGAAAAAGCAAGATTGTGCTTATTTGGTTCTTCTAAGAATGGATTTGGATTCCGAGATAGTGATCT
GGATATTTGTATGACTCTGGAAGGCCATGAAAATGCAGAGAAATTAAACTGTAAAGAAATAATTGAAAAT
TTGGCGAAAATTCTTAAGAGGCATCCAGGTTTAAGAAACATTCTGCCTATAACTACTGCCAAAGTGCCTA
TTGTAAAATTTGAACACAGGAGAAGTGGACTAGAAGGTGATATCAGTTTATATAACACATTGGCTCAACA
CAACACAAGAATGCTAGCTACTTATGCAGCTATTGATCCTAGAGTACAGTACTTGGGATATACTATGAAA
GTGTTTGCAAAGCGCTGTGACATTGGAGATGCATCTCGGGGAAGTTTATCTTCATATGCTTACATCCTTA
TGGTGCTGTACTTTCTGCAGCAAAGAAAGCCACCAGTTATCCCAGTTCTACAAGAGATCTTTGATGGGAA
GCAGATTCCGCAGAGAATGGTTGATGGATGGAATGCCTTTTTCTTTGATAAAACAGAAGAGCTGAAAAAG
CGTTTACCTTCACTTGGGAAGAACACGGAATCACTGGGAGAGCTTTGGTTAGGACTGCTTCGCTTCTATA
CAGAAGAGTTTGATTTTAAGGAGTATGTAATCAGTATTCGGCAGAAGAAGCTGCTGACCACTTTTGAAAA
GCAGTGGACTTCTAAATGCATTGCAATTGAAGATCCTTTTGACTTGAATCATAACCTTGGTGCTGGTGTT
TCTAGAAAAATGACTAATTTTATTATGAAGGCGTTTATCAATGGAAGGAAACTTTTTGGTACCCCCTTTT
ATCCACTCATTGGCAGAGAAGCTGAGTACTTCTTTGATTCAAGAGTACTAACAGATGGAGAGTTGGCTCC
TAATGATCGTTGTTGCCGTGTGTGTGGAAAAATAGGTCACTACATGAAAGACTGTCCCAAAAGGAAAAGA
CTAAAGAAGAAAGACAGTGAAGAAGAGAAGGAAGGGAACGAAGAAGAAAAAGACTCCCGTGACCTTCTTG
ACTCCCGAGACCTTAGGTGTTTCATATGTGGAGATGCTGGACATGTTCGCCGCGAATGCCCAGAGGTCAA
GATGGCCCGCCAGAGGAATAGTAGTGTGGCAGCAGCCCAGCTGGTCCGTAACCTTGTAAATGCTCAGCAG
GTGGCTGGTTCAGCGCAGCAACAGAGTGACCAGTCCATAAGGACCAGACAGTCTTCAGAATGTTCTGATT
CACCATCTTATTCTCCTCAGCCTCAGCCTTTTCCACAGAATTCTCCCCAACCATCTGCTCTTCCCCCACC
TCCATCCCAGCCAGGATCCCAGCCTAAGCTTGGCCCACCCCAGCAGGGAGGGCAGCCCCCTCATCAGGTT
CAGATGCCCTTGTATAACTTTCCTCAGTCACCTCCAGCTCATTATTCACCCATGCACAGTATGGGGTTAC
TGCCAATGCACCCCCTGCAGATCCCTGCCCCATCGTGGCCCATCCACGGCCCGATGCTGCACTCTGCACC
TGGCAGCACCCCCAGCAACATTGGCTTGAATGATCCCAGCATCATCTTTGCACAGCCTGCTGCCAGGCCC
ATGGCAATTCCCAGCCCTTCTCACGATGGACACTGGCCTCGGACCGTGGCTCCCAATTCCCTGGTGAACA
ATGGTGCAGTGGGTAATTCGGAGCCACGCTTTCGAGGACTAAATCCTCCAATTCCATGGGAACATGCACC
ACGCCATTTCCCCCTTGTCCCAGCTTCGTGGCCTTACGGTTTGCATCAAAATTTCATGCATCAGGGAAAT
CCAAGATTTCAGCCCAAACCCTTCTATGCTCAAGCAGACAGATGTGCCACGCGTCGCTGTAGAGAGCGCT
GTCCCCACCCACCACGAGGAAACGTGTCCGAGTAA


ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA
TTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-NotI     
ACCN NM_175472
Insert Size 4935 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_175472.3, NP_780681.2
RefSeq Size 6054 bp
RefSeq ORF 4935 bp
Locus ID 230594
UniProt ID B2RX14
Gene Summary Uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay (PubMed:28792939). Essential for both oocyte maturation and fertility. Through 3' terminal uridylation of mRNA, sculpts, with TUT7, the maternal transcriptome by eliminating transcripts during oocyte growth (PubMed:28792939). Involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets. Also functions as an integral regulator of microRNA biogenesiS using 3 different uridylation mechanisms (By similarity). Acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7), miR107, miR-143 and miR-200c. Uridylated miRNAs are not processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cell pluripotency (By similarity). Also catalyzes the 3' uridylation of miR-26A, a miRNA that targets IL6 transcript. This abrogates the silencing of IL6 transcript, hence promoting cytokine expression (PubMed:19703396). In the absence of LIN28A, TUT7 and TUT4 monouridylate group II pre-miRNAs, which includes most of pre-let7 members, that shapes an optimal 3' end overhang for efficient processing (PubMed:28671666). Add oligo-U tails to truncated pre-miRNAS with a 5' overhang which may promote rapid degradation of non-functional pre-miRNA species (By similarity). May also suppress Toll-like receptor-induced NF-kappa-B activation via binding to T2BP (By similarity). Does not play a role in replication-dependent histone mRNA degradation (By similarity). Due to functional redundancy between TUT4 and TUT7, the identification of the specific role of each of these proteins is difficult (PubMed:28671666, PubMed:28792939, PubMed:22898984). TUT4 and TUT7 restrict retrotransposition of long interspersed element-1 (LINE-1) in cooperation with MOV10 counteracting the RNA chaperonne activity of L1RE1. TUT7 uridylates LINE-1 mRNAs in the cytoplasm which inhibits initiation of reverse transcription once in the nucleus, whereas uridylation by TUT4 destabilizes mRNAs in cytoplasmic ribonucleoprotein granules (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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