Kdm6b (NM_001017426) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1700064E03Rik; BC038313; Jmjd3; mKIAA0346
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224778 representing NM_001017426 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATCGGGCAGTGGACCCTCCAGGGGCCCGCTCTGCACGGGAAGCCTTTGCCCTTGGGGGCTTGAGCT GTGCTGGGGCTTGGAGCTCCTGCCCACCCCATCCTCCTCCCCGAAGCTCATGGCTGCCCGGAGGCAGATG CTCTGCCAGCGTTGGGCAGCCCCCACTCTCAGCTCCTTTACCCCCATCTCATGGCAGTAGCTCCGGGCAC CCTAACAAACCCTATTATGCTCCTGGGACACCCACCCCAAGACCCCTTCACGGGAAGTTGGAATCCCTAC ATGGCTGTGTCCAGGCATTGCTCCGGGAGCCAGCGCAGCCAGGGTTGTGGGAACAGCTTGGACAGTTGTA TGAATCAGAGCACGACAGTGAGGAAGCCGTATGCTGCTACCATAGGGCCCTTCGCTATGGAGGAAGCTTC GCCGAGCTGGGACCCCGGATTGGCCGCTTGCAGCAGGCCCAGCTCTGGAACTTTCATGCCGGTTCCTGTC AGCACAGAGCCAAGGTCCTGCCTCCCCTGGAGCAAGTCTGGAATTTGCTGCACCTTGAGCACAAACGGAA CTATGGGGCTAAGCGAGGGGGCCCTCCAGTGAAGAGATCTGCTGAACCCCCCGTGGTCCAGCCTATGCCT CCTGCAGCCCTCTCAGGCCCCTCAGGAGAGGAGGGCCTTAGCCCTGGAGGCAAGCGCAGGAGAGGCTGCA GCTCTGAACAGGCTGGCCTTCCCCCAGGTCTGCCACTCCCTCCACCACCCCCACCCCCACCGCCTCCACC ACCACCACCACCCCCTCCACCACCACCGCTGCCTGGCCTGGCTATTAGCCCCCCATTTCAGCTGACTAAG CCAGGGCTGTGGAATACCCTGCATGGAGATGCTTGGGGCCCCGAGCGCAAGGGTTCAGCGCCGCCAGAGC GCCAGGAGCAGCGGCACTCGATGCCTCATTCATATCCATACCCAGCTCCCGCCTACTCCGCTCATCCGCC CAGCCATCGGCTGGTCCCCAACACACCCCTTGGTCCAGGTCCCCGACCCCCAGGAGCAGAGAGCCATGGC TGCCTGCCTGCCACCCGTCCCCCCGGAAGTGACCTTAGAGAGAGCAGAGTTCAGAGGTCGCGGATGGACT CCAGCGTTTCACCAGCAGCATCTACCGCCTGCGTGCCTTACGCCCCTTCCCGGCCCCCTGGCCTCCCCGG CACCAGCAGCAGCAGCAGCAGCAGCAGTAGCAGTAACAACACTGGTCTTCGGGGTGTGGAGCCAAGCCCA GGCATTCCTGGCGCTGACCATTACCAAAACCCTGCGCTGGAGATATCCCCTCACCAGGCCCGCCTGGGTC CCTCCGCACACAGCAGTCGGAAACCATTTTTGACGGCCCCTGCTGCCACGCCCCACTTATCCCTACCCCC TGGGACCCCATCATCCCCTCCACCCCCATGTCCTCGCCTCTTGCGCCCTCCACCGCCCCCTGCTTGGATG AAGGGCTCAGCCTGCCGTGCAGCCCGAGAGGATGGAGAGATCTTAGGGGAGCTCTTCTTTGGTGCTGAGG GACCTCCCCGTCCTCCTCCCCCACCCCTTCCCCACCGTGATGGCTTCTTGGGGCCTCCAAACCCCCGCTT TTCTGTGGGCACTCAGGATTCGCATAACCCTCCCATTCCCCCAACCACCACCAGCAGCAGCAGCAGCAGC AACAGCCACAGCAGTAGTCCTACTGGGCCGGTGCCCTTTCCACCACCCTCCTATCTGGCCAGAAGTATAG ACCCCCTCCCCAGGCCATCCAGCCCAACCTTGAGCCCCCAGGACCCACCTCTTCCACCACTGACTCTTGC CCTGCCTCCAGCCCCTCCCTCCTCCTGCCACCAAAATACCTCAGGAAGCTTCAGGCGCTCGGAGAGCCCC CGGCCCAGGGTCTCCTTCCCAAAGACCCCCGAGGTGGGGCAGGGGCCACCCCCAGGCCCTGTGAGTAAAG CCCCCCAGCCTGTGCCACCTGGGGTTGGAGAGCTGCCTGCCCGAGGCCCGAGGCTCTTTGATTTCCCACC CACTCCGCTGGAGGACCAGTTTGAAGAGCCAGCCGAATTCAAGATCCTACCTGATGGGCTGGCAAACATC ATGAAGATGCTGGATGAATCCATTCGGAAGGAGGAGGAGCAGCAGCAGCAGCAGGAGGCAGGCGTGGCTC CCCCACCCCCACTCAAAGAGCCCTTTGCATCTCTACAGCCTCCATTTCCCAGTGACACAGCCCCAGCCAC CACCACTGCTGCCCCCACCACCGCCACCACCACCACAACCACCACCACCACCACCACCCAAGAAGAGGAG AAGAAGCCACCACCAGCCCTACCACCACCACCGCCTCTAGCCAAGTTTCCTCCACCTCCCCAGCCACAGC CCCCACCACCTCCACCAGCCAGCCCAGCCAGCCTGCTCAAATCGTTGGCCTCTGTTCTTGAGGGACAAAA GTACTGTTACCGGGGGACTGGAGCAGCCGTCTCAACCAGGCCCGGGTCCGTGCCCGCCACTCAGTATTCC CCTAGTCCTGCATCAGGTGCTACCGCCCCACCACCCACTTCAGTGGCCCCTAGTGCCCAGGGCTCCCCCA AGCCCTCGGTTTCCTCGTCATCTCAGTTCTCTACCTCAGGCGGGCCTTGGGCCCGGGAGCACAGGGCGGG TGAAGAGCCAGCACCAGGCCCCGTGACCCCTGCCCAGTTGCCCCCACCTCTGCCGCTGCCCCCTGCTCGT TCTGAGTCTGAGGTGCTAGAAGAAATCAGTCGGGCTTGTGAGACCCTTGTAGAGCGGGTGGGCCGGAGTG CCATCAACCCAGTGGACACGGCAGACCCAGTGGACAGTGGGACTGAGCCACAGCCGCCGCCTGCGCAGGC CAAGGAGGAGAGTGGGGGGGTGGCGGTAGCAGCAGCAGGTCCAGGTAGTGGCAAGCGTCGTCAAAAGGAG CATCGGCGGCACAGGCGGGCCTGTAGGGACAGTGTGGGTCGACGACCCCGCGAGGGGAGGGCCAAGGCCA AGGCCAAGGCTCCCAAAGAAAAAAGCCGAAGGGTGCTGGGGAACCTCGACTTGCAGAGTGAGGAGATCCA GGGCCGGGAGAAGGCCCGGCCCGATGTCGGTGGGGTTTCCAAAGTCAAGACACCCACAGCTCCAGCACCC CCGCCTGCTCCTGCACCCGCTGCTCAGCCAACACCCCCATCAGCTCCTGTCCCTGGGAAGAAGACTCGTG AGGAGGCTCCGGGGCCTCCAGGTGTGAGCCGGGCAGATATGCTGAAGCTCCGGTCACTTAGTGAGGGGCC TCCCAAGGAGCTGAAGATCAGGCTCATCAAGGTGGAAAGTGGGGACAAGGAGACCTTTATCGCCTCTGAG GTGGAAGAGCGGCGGCTGCGCATGGCAGACCTCACCATCAGCCACTGTGCCGCCGATGTCATGCGTGCCA GCAAGAATGCCAAGGTGAAAGGGAAATTCCGAGAGTCCTACCTGTCCCCTGCCCAGTCTGTGAAACCCAA GATCAACACTGAGGAGAAGCTGCCCCGGGAAAAACTCAACCCCCCTACCCCCAGCATCTATTTGGAGAGC AAACGAGATGCCTTCTCGCCGGTCCTGCTACAGTTCTGTACAGACCCCCGGAACCCCATCACCGTCATCA GGGGCCTGGCTGGTTCACTTCGGCTCAACTTAGGCCTTTTCTCCACCAAGACTCTGGTGGAGGCGAGCGG TGAACATACGGTGGAGGTCCGTACCCAAGTACAGCAGCCCTCAGACGAGAACTGGGACCTGACAGGTACC AGACAAATCTGGCCCTGTGAGAGCTCCCGTTCCCACACCACCATCGCTAAATACGCACAGTACCAGGCCT CGTCCTTCCAGGAGTCACTGCAGGAGGAGAGGGAGAGTGAGGATGAGGAATCCGAGGAACCAGACAGCAC TACAGGAACCTCTCCCAGCAGTGCACCGGACCCCAAGAACCATCACATCATCAAGTTTGGCACTAACATC GACCTGTCTGATGCCAAGAGGTGGAAGCCACAGCTACAGGAGCTGCTGAAACTGCCCGCCTTCATGCGGG TAACATCCACAGGCAACATGCTCAGCCACGTGGGCCACACCATCCTGGGCATGAACACCGTGCAGCTATA CATGAAGGTCCCTGGCAGCCGAACGCCAGGCCACCAAGAGAATAACAATTTCTGCTCAGTCAACATCAAC ATTGGCCCTGGGGACTGCGAGTGGTTCGCGGTACATGAGCACTATTGGGAGACCATCAGCGCCTTCTGCG ACCGGCATGGTGTGGACTACTTGACTGGTTCCTGGTGGCCAATCTTGGATGACCTCTATGCGTCCAATAT TCCTGTTTACCGCTTCGTGCAGCGCCCTGGAGACCTTGTGTGGATTAATGCAGGGACTGTACATTGGGTG CAGGCTACCGGCTGGTGCAACAACATTGCCTGGAACGTGGGGCCCCTCACCGCCTATCAGTACCAGCTGG CCCTGGAGCGATATGAGTGGAACGAGGTGAAGAACGTCAAGTCCATTGTGCCCATGATTCATGTGTCCTG GAACGTCGCTCGAACGGTCAAGATCAGCGATCCTGACTTGTTCAAGATGATCAAGTTCTGCCTCCTGCAG TCAATGAAGCACTGTCAGGTACAGCGGGAGAGCCTGGTGCGGGCAGGGAAGAAGATCGCTTACCAAGGCC GTGTCAAAGACGAGCCTGCCTACTACTGCAACGAATGCGACGTGGAGGTGTTCAACATCCTGTTCGTTAC AAGTGAGAATGGCAGCCGAAACACGTACCTGGTGCACTGCGAGGGCTGTGCGCGCCGTCGCAGCGCGGGC CTACAGGGCGTGGTGGTGCTAGAGCAGTACCGCACGGAGGAGCTGGCGCAGGCCTACGATGCCTTCACAC TGGCTCCCGCCAGCACGTCTCGATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001017426
Insert Size	4926 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001017426.1, NP_001017426.1
RefSeq Size	6654 bp
RefSeq ORF	4926 bp
Locus ID	216850
UniProt ID	Q5NCY0
Gene Summary	Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation (PubMed:17825402). Plays a demethylase-independent role in chromatin remodeling to regulate T-box family member-dependent gene expression by acting as a link between T-box factors and the SMARCA4-containing SWI/SNF remodeling complex (PubMed:21095589).[UniProtKB/Swiss-Prot Function]

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