Kdm6b (NM_001017426) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	1700064E03Rik; BC038313; Jmjd3; mKIAA0346
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR212060 representing NM_001017426 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATCGGGCAGTGGACCCTCCAGGGGCCCGCTCTGCACGGGAAGCCTTTGCCCTTGGGGGCTTGAGCT GTGCTGGGGCTTGGAGCTCCTGCCCACCCCATCCTCCTCCCCGAAGCTCATGGCTGCCCGGAGGCAGATG CTCTGCCAGCGTTGGGCAGCCCCCACTCTCAGCTCCTTTACCCCCATCTCATGGCAGTAGCTCCGGGCAC CCTAACAAACCCTATTATGCTCCTGGGACACCCACCCCAAGACCCCTTCACGGGAAGTTGGAATCCCTAC ATGGCTGTGTCCAGGCATTGCTCCGGGAGCCAGCGCAGCCAGGGTTGTGGGAACAGCTTGGACAGTTGTA TGAATCAGAGCACGACAGTGAGGAAGCCGTATGCTGCTACCATAGGGCCCTTCGCTATGGAGGAAGCTTC GCCGAGCTGGGACCCCGGATTGGCCGCTTGCAGCAGGCCCAGCTCTGGAACTTTCATGCCGGTTCCTGTC AGCACAGAGCCAAGGTCCTGCCTCCCCTGGAGCAAGTCTGGAATTTGCTGCACCTTGAGCACAAACGGAA CTATGGGGCTAAGCGAGGGGGCCCTCCAGTGAAGAGATCTGCTGAACCCCCCGTGGTCCAGCCTATGCCT CCTGCAGCCCTCTCAGGCCCCTCAGGAGAGGAGGGCCTTAGCCCTGGAGGCAAGCGCAGGAGAGGCTGCA GCTCTGAACAGGCTGGCCTTCCCCCAGGTCTGCCACTCCCTCCACCACCCCCACCCCCACCGCCTCCACC ACCACCACCACCCCCTCCACCACCACCGCTGCCTGGCCTGGCTATTAGCCCCCCATTTCAGCTGACTAAG CCAGGGCTGTGGAATACCCTGCATGGAGATGCTTGGGGCCCCGAGCGCAAGGGTTCAGCGCCGCCAGAGC GCCAGGAGCAGCGGCACTCGATGCCTCATTCATATCCATACCCAGCTCCCGCCTACTCCGCTCATCCGCC CAGCCATCGGCTGGTCCCCAACACACCCCTTGGTCCAGGTCCCCGACCCCCAGGAGCAGAGAGCCATGGC TGCCTGCCTGCCACCCGTCCCCCCGGAAGTGACCTTAGAGAGAGCAGAGTTCAGAGGTCGCGGATGGACT CCAGCGTTTCACCAGCAGCATCTACCGCCTGCGTGCCTTACGCCCCTTCCCGGCCCCCTGGCCTCCCCGG CACCAGCAGCAGCAGCAGCAGCAGCAGTAGCAGTAACAACACTGGTCTTCGGGGTGTGGAGCCAAGCCCA GGCATTCCTGGCGCTGACCATTACCAAAACCCTGCGCTGGAGATATCCCCTCACCAGGCCCGCCTGGGTC CCTCCGCACACAGCAGTCGGAAACCATTTTTGACGGCCCCTGCTGCCACGCCCCACTTATCCCTACCCCC TGGGACCCCATCATCCCCTCCACCCCCATGTCCTCGCCTCTTGCGCCCTCCACCGCCCCCTGCTTGGATG AAGGGCTCAGCCTGCCGTGCAGCCCGAGAGGATGGAGAGATCTTAGGGGAGCTCTTCTTTGGTGCTGAGG GACCTCCCCGTCCTCCTCCCCCACCCCTTCCCCACCGTGATGGCTTCTTGGGGCCTCCAAACCCCCGCTT TTCTGTGGGCACTCAGGATTCGCATAACCCTCCCATTCCCCCAACCACCACCAGCAGCAGCAGCAGCAGC AACAGCCACAGCAGTAGTCCTACTGGGCCGGTGCCCTTTCCACCACCCTCCTATCTGGCCAGAAGTATAG ACCCCCTCCCCAGGCCATCCAGCCCAACCTTGAGCCCCCAGGACCCACCTCTTCCACCACTGACTCTTGC CCTGCCTCCAGCCCCTCCCTCCTCCTGCCACCAAAATACCTCAGGAAGCTTCAGGCGCTCGGAGAGCCCC CGGCCCAGGGTCTCCTTCCCAAAGACCCCCGAGGTGGGGCAGGGGCCACCCCCAGGCCCTGTGAGTAAAG CCCCCCAGCCTGTGCCACCTGGGGTTGGAGAGCTGCCTGCCCGAGGCCCGAGGCTCTTTGATTTCCCACC CACTCCGCTGGAGGACCAGTTTGAAGAGCCAGCCGAATTCAAGATCCTACCTGATGGGCTGGCAAACATC ATGAAGATGCTGGATGAATCCATTCGGAAGGAGGAGGAGCAGCAGCAGCAGCAGGAGGCAGGCGTGGCTC CCCCACCCCCACTCAAAGAGCCCTTTGCATCTCTACAGCCTCCATTTCCCAGTGACACAGCCCCAGCCAC CACCACTGCTGCCCCCACCACCGCCACCACCACCACAACCACCACCACCACCACCACCCAAGAAGAGGAG AAGAAGCCACCACCAGCCCTACCACCACCACCGCCTCTAGCCAAGTTTCCTCCACCTCCCCAGCCACAGC CCCCACCACCTCCACCAGCCAGCCCAGCCAGCCTGCTCAAATCGTTGGCCTCTGTTCTTGAGGGACAAAA GTACTGTTACCGGGGGACTGGAGCAGCCGTCTCAACCAGGCCCGGGTCCGTGCCCGCCACTCAGTATTCC CCTAGTCCTGCATCAGGTGCTACCGCCCCACCACCCACTTCAGTGGCCCCTAGTGCCCAGGGCTCCCCCA AGCCCTCGGTTTCCTCGTCATCTCAGTTCTCTACCTCAGGCGGGCCTTGGGCCCGGGAGCACAGGGCGGG TGAAGAGCCAGCACCAGGCCCCGTGACCCCTGCCCAGTTGCCCCCACCTCTGCCGCTGCCCCCTGCTCGT TCTGAGTCTGAGGTGCTAGAAGAAATCAGTCGGGCTTGTGAGACCCTTGTAGAGCGGGTGGGCCGGAGTG CCATCAACCCAGTGGACACGGCAGACCCAGTGGACAGTGGGACTGAGCCACAGCCGCCGCCTGCGCAGGC CAAGGAGGAGAGTGGGGGGGTGGCGGTAGCAGCAGCAGGTCCAGGTAGTGGCAAGCGTCGTCAAAAGGAG CATCGGCGGCACAGGCGGGCCTGTAGGGACAGTGTGGGTCGACGACCCCGCGAGGGGAGGGCCAAGGCCA AGGCCAAGGCTCCCAAAGAAAAAAGCCGAAGGGTGCTGGGGAACCTCGACTTGCAGAGTGAGGAGATCCA GGGCCGGGAGAAGGCCCGGCCCGATGTCGGTGGGGTTTCCAAAGTCAAGACACCCACAGCTCCAGCACCC CCGCCTGCTCCTGCACCCGCTGCTCAGCCAACACCCCCATCAGCTCCTGTCCCTGGGAAGAAGACTCGTG AGGAGGCTCCGGGGCCTCCAGGTGTGAGCCGGGCAGATATGCTGAAGCTCCGGTCACTTAGTGAGGGGCC TCCCAAGGAGCTGAAGATCAGGCTCATCAAGGTGGAAAGTGGGGACAAGGAGACCTTTATCGCCTCTGAG GTGGAAGAGCGGCGGCTGCGCATGGCAGACCTCACCATCAGCCACTGTGCCGCCGATGTCATGCGTGCCA GCAAGAATGCCAAGGTGAAAGGGAAATTCCGAGAGTCCTACCTGTCCCCTGCCCAGTCTGTGAAACCCAA GATCAACACTGAGGAGAAGCTGCCCCGGGAAAAACTCAACCCCCCTACCCCCAGCATCTATTTGGAGAGC AAACGAGATGCCTTCTCGCCGGTCCTGCTACAGTTCTGTACAGACCCCCGGAACCCCATCACCGTCATCA GGGGCCTGGCTGGTTCACTTCGGCTCAACTTAGGCCTTTTCTCCACCAAGACTCTGGTGGAGGCGAGCGG TGAACATACGGTGGAGGTCCGTACCCAAGTACAGCAGCCCTCAGACGAGAACTGGGACCTGACAGGTACC AGACAAATCTGGCCCTGTGAGAGCTCCCGTTCCCACACCACCATCGCTAAATACGCACAGTACCAGGCCT CGTCCTTCCAGGAGTCACTGCAGGAGGAGAGGGAGAGTGAGGATGAGGAATCCGAGGAACCAGACAGCAC TACAGGAACCTCTCCCAGCAGTGCACCGGACCCCAAGAACCATCACATCATCAAGTTTGGCACTAACATC GACCTGTCTGATGCCAAGAGGTGGAAGCCACAGCTACAGGAGCTGCTGAAACTGCCCGCCTTCATGCGGG TAACATCCACAGGCAACATGCTCAGCCACGTGGGCCACACCATCCTGGGCATGAACACCGTGCAGCTATA CATGAAGGTCCCTGGCAGCCGAACGCCAGGCCACCAAGAGAATAACAATTTCTGCTCAGTCAACATCAAC ATTGGCCCTGGGGACTGCGAGTGGTTCGCGGTACATGAGCACTATTGGGAGACCATCAGCGCCTTCTGCG ACCGGCATGGTGTGGACTACTTGACTGGTTCCTGGTGGCCAATCTTGGATGACCTCTATGCGTCCAATAT TCCTGTTTACCGCTTCGTGCAGCGCCCTGGAGACCTTGTGTGGATTAATGCAGGGACTGTACATTGGGTG CAGGCTACCGGCTGGTGCAACAACATTGCCTGGAACGTGGGGCCCCTCACCGCCTATCAGTACCAGCTGG CCCTGGAGCGATATGAGTGGAACGAGGTGAAGAACGTCAAGTCCATTGTGCCCATGATTCATGTGTCCTG GAACGTCGCTCGAACGGTCAAGATCAGCGATCCTGACTTGTTCAAGATGATCAAGTTCTGCCTCCTGCAG TCAATGAAGCACTGTCAGGTACAGCGGGAGAGCCTGGTGCGGGCAGGGAAGAAGATCGCTTACCAAGGCC GTGTCAAAGACGAGCCTGCCTACTACTGCAACGAATGCGACGTGGAGGTGTTCAACATCCTGTTCGTTAC AAGTGAGAATGGCAGCCGAAACACGTACCTGGTGCACTGCGAGGGCTGTGCGCGCCGTCGCAGCGCGGGC CTACAGGGCGTGGTGGTGCTAGAGCAGTACCGCACGGAGGAGCTGGCGCAGGCCTACGATGCCTTCACAC TGGCTCCCGCCAGCACGTCTCGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR212060 representing NM_001017426 Red=Cloning site Green=Tags(s) MHRAVDPPGARSAREAFALGGLSCAGAWSSCPPHPPPRSSWLPGGRCSASVGQPPLSAPLPPSHGSSSGH PNKPYYAPGTPTPRPLHGKLESLHGCVQALLREPAQPGLWEQLGQLYESEHDSEEAVCCYHRALRYGGSF AELGPRIGRLQQAQLWNFHAGSCQHRAKVLPPLEQVWNLLHLEHKRNYGAKRGGPPVKRSAEPPVVQPMP PAALSGPSGEEGLSPGGKRRRGCSSEQAGLPPGLPLPPPPPPPPPPPPPPPPPPPPLPGLAISPPFQLTK PGLWNTLHGDAWGPERKGSAPPERQEQRHSMPHSYPYPAPAYSAHPPSHRLVPNTPLGPGPRPPGAESHG CLPATRPPGSDLRESRVQRSRMDSSVSPAASTACVPYAPSRPPGLPGTSSSSSSSSSSNNTGLRGVEPSP GIPGADHYQNPALEISPHQARLGPSAHSSRKPFLTAPAATPHLSLPPGTPSSPPPPCPRLLRPPPPPAWM KGSACRAAREDGEILGELFFGAEGPPRPPPPPLPHRDGFLGPPNPRFSVGTQDSHNPPIPPTTTSSSSSS NSHSSSPTGPVPFPPPSYLARSIDPLPRPSSPTLSPQDPPLPPLTLALPPAPPSSCHQNTSGSFRRSESP RPRVSFPKTPEVGQGPPPGPVSKAPQPVPPGVGELPARGPRLFDFPPTPLEDQFEEPAEFKILPDGLANI MKMLDESIRKEEEQQQQQEAGVAPPPPLKEPFASLQPPFPSDTAPATTTAAPTTATTTTTTTTTTTQEEE KKPPPALPPPPPLAKFPPPPQPQPPPPPPASPASLLKSLASVLEGQKYCYRGTGAAVSTRPGSVPATQYS PSPASGATAPPPTSVAPSAQGSPKPSVSSSSQFSTSGGPWAREHRAGEEPAPGPVTPAQLPPPLPLPPAR SESEVLEEISRACETLVERVGRSAINPVDTADPVDSGTEPQPPPAQAKEESGGVAVAAAGPGSGKRRQKE HRRHRRACRDSVGRRPREGRAKAKAKAPKEKSRRVLGNLDLQSEEIQGREKARPDVGGVSKVKTPTAPAP PPAPAPAAQPTPPSAPVPGKKTREEAPGPPGVSRADMLKLRSLSEGPPKELKIRLIKVESGDKETFIASE VEERRLRMADLTISHCAADVMRASKNAKVKGKFRESYLSPAQSVKPKINTEEKLPREKLNPPTPSIYLES KRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGT RQIWPCESSRSHTTIAKYAQYQASSFQESLQEERESEDEESEEPDSTTGTSPSSAPDPKNHHIIKFGTNI DLSDAKRWKPQLQELLKLPAFMRVTSTGNMLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNIN IGPGDCEWFAVHEHYWETISAFCDRHGVDYLTGSWWPILDDLYASNIPVYRFVQRPGDLVWINAGTVHWV QATGWCNNIAWNVGPLTAYQYQLALERYEWNEVKNVKSIVPMIHVSWNVARTVKISDPDLFKMIKFCLLQ SMKHCQVQRESLVRAGKKIAYQGRVKDEPAYYCNECDVEVFNILFVTSENGSRNTYLVHCEGCARRRSAG LQGVVVLEQYRTEELAQAYDAFTLAPASTSR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001017426
ORF Size	4923 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001017426.2
RefSeq Size	6654 bp
RefSeq ORF	4926 bp
Locus ID	216850
UniProt ID	Q5NCY0
MW	176.8 kDa
Gene Summary	Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation (PubMed:17825402). Plays a demethylase-independent role in chromatin remodeling to regulate T-box family member-dependent gene expression by acting as a link between T-box factors and the SMARCA4-containing SWI/SNF remodeling complex (PubMed:21095589).[UniProtKB/Swiss-Prot Function]

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