Robo1 (NM_019413) Mouse Untagged Clone
CAT#: MC224754
Robo1 (untagged) - Mouse roundabout homolog 1 (Drosophila) (Robo1), (10ug)
CNY 11,040.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | AW494633; AW742721; DUTT1; Gm310 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC224754 representing NM_019413
Red=Cloning site Blue=ORF TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATCGCGGAGCCTGCTCGCTTTTACCTCTTTGGATTAATATGTCTCTGTTCAGGCTCCCGTCTTCGTC AGGAAGATTTTCCACCCCGCATTGTTGAACACCCTTCAGACCTGATCGTCTCCAAAGGAGAACCTGCCAC TCTGAACTGTAAAGCCGAAGGCCGCCCCACCCCGACGATAGAATGGTACAAAGGTGGGGAAAGAGTGGAG ACGGACAAAGATGACCCGCGCTCACACCGGATGCTGCTGCCAAGCGGGTCTTTATTTTTCTTACGCATAG TCCATGGACGGAAGAGTAGGCCCGACGAAGGGGTCTACATCTGTGTGGCTAGGAATTACCTCGGAGAGGC TGTGAGCCACAACGCATCGCTGGAGGTAGCTATACTACGGGATGACTTCAGACAAAACCCTTCAGATGTC ATGGTGGCAGTAGGCGAACCTGCAGTCATGGAATGCCAGCCTCCACGGGGCCATCCAGAGCCCACCATCT CCTGGAAAAAGGATGGCTCACCCCTGGATGATAAAGATGAAAGAATCACTATACGAGGAGGAAAACTCAT GATCACTTATACGCGTAAAAGCGATGCTGGCAAGTATGTTTGCGTTGGCACCAACATGGTGGGGGAACGG GAGAGTGAAGTCGCAGAGCTAACCGTTTTAGAGAGACCATCGTTTGTGAAGAGACCCAGTAATTTGGCAG TGACTGTGGATGACAGTGCAGAATTTAAATGTGAGGCCCGAGGAGACCCTGTGCCTACAGTTCGATGGAG GAAAGATGACGGAGAGCTGCCCAAATCCAGATATGAAATCCGAGATGATCATACTTTGAAAATTCGGAAG GTGACTGCTGGCGACATGGGATCATACACTTGTGTGGCAGAAAATATGGTCGGGAAAGCTGAAGCTTCCG CTACTTTGACAGTTCAAGAGCCGCCACATTTCGTTGTAAAACCTCGGGACCAGGTGGTTGCCTTGGGAAG AACTGTGACGTTTCAGTGCGAAGCAACTGGAAATCCTCAACCAGCTATCTTTTGGAGGAGAGAGGGGAGT CAGAATCTACTTTTCTCCTACCAGCCTCCACAGTCCTCTAGCCGATTTTCAGTTTCGCAGACAGGAGACC TCACCATTACGAACGTCCAGCGGTCTGATGTCGGCTACTACATCTGTCAGACTTTAAATGTTGCTGGAAG TATCATCACAAAGGCCTATTTGGAAGTTACTGATGTGATTGCAGACCGGCCTCCCCCAGTCATTCGGCAA GGTCCTGTGAATCAGACTGTAGCAGTGGATGGCACTTTAATCCTCAGCTGTGTGGCCACAGGCAGTCCAG CACCCACAATTCTGTGGAGGAAGGATGGAGTCCTTGTTTCAACCCAAGATTCTCGGATCAAACAGCTGGA GAGTGGCGTGCTGCAGATCCGCTACGCTAAGCTGGGTGACACGGGTCGGTACACCTGCACCGCATCGACC CCCAGCGGTGAGGCCACGTGGAGCGCTTACATCGAAGTCCAGGAATTTGGGGTTCCAGTTCAACCCCCAA GACCCACCGACCCCAACTTAATCCCCAGCGCCCCCTCAAAGCCCGAAGTTACAGATGTCAGCAAAAACAC GGTCACTTTATCGTGGCAGCCAAACTTGAACTCAGGAGCAACCCCAACATCTTATATTATAGAAGCCTTC AGCCATGCATCTGGTAGCAGCTGGCAGACGGCGGCAGAAAATGTGAAAACAGAAACATTTGCCATCAAAG GACTCAAACCTAACGCCATTTACCTTTTCCTGGTGAGGGCAGCAAATGCCTATGGAATTAGTGATCCAAG CCAAATATCAGATCCAGTGAAAACGCAAGATGTCCCACCAACCAGCCAAGGGGTGGACCACAAGCAGGTC CAAAGAGAACTGGGGAATGTTGTCTTACATCTGCACAACCCGACCATCCTTTCTTCCTCCTCAGTCGAAG TGCACTGGACGGTGGACCAACAATCTCAATATATTCAAGGCTATAAAATTCTCTACCGCCCATCTGGAGC CAGCCATGGTGAATCGGAGTGGTTAGTTTTTGAAGTGAGGACGCCAACGAAAAACAGCGTGGTGATCCCC GATCTCAGAAAAGGCGTCAACTACGAGATTAAGGCTCGCCCATTTTTTAATGAGTTTCAAGGAGCAGACA GTGAAATCAAATTTGCCAAAACCTTAGAAGAAGCACCAAGTGCCCCTCCCCGAAGTGTAACTGTATCCAA GAATGATGGAAATGGGACTGCAATCCTGGTCACTTGGCAACCGCCTCCTGAAGACACACAAAATGGAATG GTCCAAGAATATAAGGTTTGGTGTCTCGGTAATGAAACGAAGTATCACATAAACAAGACAGTCGATGGCT CGACCTTCTCGGTGGTCATCCCCTCTCTGGTTCCTGGGATCCGATACAGTGTGGAGGTGGCAGCGAGCAC TGGGGCGGGTCCCGGGGTGAAGAGCGAACCTCAGTTCATCCAGTTAGATTCTCACGGAAACCCCGTGTCT CCCGAGGACCAAGTCAGCCTTGCTCAGCAGATCTCCGACGTGGTGAGGCAGCCGGCGTTCATTGCAGGCA TTGGGGCAGCCTGTTGGATTATCCTCATGGTCTTCAGCATCTGGCTTTACAGACACCGCAAGAAGAGAAA CGGACTCACCAGCACGTATGCGGGTATCCGGAAAGTCCCGTCCTTTACCTTCACGCCAACAGTAACTTAT CAAAGAGGAGGCGAAGCCGTCAGCAGTGGAGGGAGGCCGGGACTCCTCAACATTAGTGAACCTGCCACGC AGCCATGGCTGGCAGACACGTGGCCCAACACAGGCAACAACCACAATGACTGCTCCATCAACTGCTGCAC GGCCGGCAATGGCAACAGCGACAGCAACCTGACAACCTACAGTCGCCCAGCTGATTGTATAGCCAATTAT AACAACCAACTGGATAACAAACAAACAAATCTGATGCTCCCTGAGTCAACTGTTTATGGTGATGTGGACC TTAGTAACAAAATCAATGAGATGAAAACCTTCAATAGCCCAAATCTGAAGGACGGGCGTTTTGTCAATCC ATCAGGGCAGCCCACTCCCTATGCCACCACTCAACTCATCCAGGCGAACCTCAGCAACAATATGAATAAT GGCGCCGGGGACTCCAGCGAGAAGCACTGGAAGCCTCCGGGACAGCAGAAACCGGAAGTGGCACCAATTC AGTATAACATCATGGAGCAAAACAAGCTGAACAAAGATTATCGGGCAAATGACACGATCCCTCCAACCAT CCCATACAACCAGTCCTATGACCAGAACACAGGAGGGTCTTATAACAGCTCAGATCGGGGCAGTAGTACA TCCGGGAGTCAAGGCCACAAGAAAGGGGCACGGACACCAAAGGCACCCAAGCAGGGTGGCATGAACTGGG CAGACTTGCTGCCCCCTCCTCCGGCGCATCCTCCCCCACATAGCAACAGTGAAGAATACAACATGTCCGT AGATGAAAGCTATGATCAAGAAATGCCCTGTCCAGTGCCACCTGCGCGGATGTATTTGCAACAGGATGAA TTAGAGGAAGAGGAAGATGAAAGAGGCCCCACTCCCCCTGTCCGGGGGGCAGCTTCTTCTCCAGCTGCTG TGTCCTACAGCCACCAATCCACGGCCACTCTCACCCCTTCTCCCCAGGAAGAGCTCCAGCCCATGTTACA GGATTGTCCGGAGGACCTCGGGCACATGCCCCACCCACCAGACAGGAGGCGGCAGCCCGTGAGTCCTCCT CCACCGCCACGGCCAATTTCCCCACCTCATACTTACGGCTACATTTCAGGACCCCTTGTCTCAGATATGG ATACCGATGCCCCAGAAGAGGAGGAGGACGAAGCTGACATGGAAGTTGCCAAAATGCAGACCCGAAGGCT GTTGTTACGTGGGCTGGAGCAGACCCCAGCATCCAGTGTTGGGGACCTTGAGAGCTCCGTCACTGGGTCC ATGATCAATGGCTGGGGCTCAGCCTCGGAAGAGGACAACATTTCCAGTGGGCGCTCCAGTGTCAGTTCAT CCGATGGCTCCTTTTTCACTGACGCTGATTTTGCCCAGGCAGTTGCTGCAGCTGCGGAGTATGCGGGCCT GAAAGTGGCTCGCCGCCAAATGCAAGATGCTGCTGGCCGCCGCCACTTCCATGCCTCTCAGTGCCCAAGG CCCACGAGTCCTGTGTCCACAGACAGCAACATGAGTGCTGTTGTGATCCAGAAAGCCAGACCCGCCAAGA AGCAGAAACACCAGCCAGGACATCTGCGCAGGGAAGCCTACGCAGATGATCTTCCACCCCCTCCAGTGCC ACCACCTGCTATAAAATCGCCCACTGTCCAGTCCAAGGCACAGCTGGAGGTACGGCCTGTCATGGTGCCA AAACTCGCGTCTATAGAAGCAAGGACAGATAGATCGTCAGACAGAAAAGGAGGCAGTTACAAGGGGAGAG AAGCTCTGGATGGAAGACAAGTCACTGACCTGCGAACAAATCCAAGTGACCCCAGAGAGGCACAGGAACA GCCAAATGACGGGAAAGGCCGAGGAACAAAGGCAGCCAAGCGAGACCTTCCACCAGCAAAGACGCACCTC GGCCAAGAGGATATTCTTCCGTACTGTAGACCTACTTTCCCAACGTCAAACAACCCTAGAGACCCCAGTT CCTCCAGCTCTATGTCATCAAGAGGATCAGGGAGCCGACAAAGAGAGCAAGCAAATGTGGGCCGAAGGAA TATGGCAGAAATGCAAGTACTTGGGGGATTTGAAAGAGGAGATGAGAATAATGAAGAATTAGAGGAAACT GAAAGCTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-RsrII |
ACCN | NM_019413 |
Insert Size | 4839 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_019413.2, NP_062286.2 |
RefSeq Size | 7568 bp |
RefSeq ORF | 4839 bp |
Locus ID | 19876 |
UniProt ID | O89026 |
Gene Summary | Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development (PubMed:10433822, PubMed:24560577). Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1 (PubMed:24560577). In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex (By similarity). Plays a role in the regulation of cell migration via its interaction with MYO9B; inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA (By similarity). May be required for lung development (PubMed:11734623).[UniProtKB/Swiss-Prot Function] |
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FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG224013 | Robo1 (tGFP-tagged) - Mouse roundabout homolog 1 (Drosophila) (Robo1), (10ug) |
CNY 15,580.00 |
|
MR224013 | Robo1 (Myc-DDK-tagged) - Mouse roundabout homolog 1 (Drosophila) (Robo1) |
CNY 11,032.00 |
|
MR224013L3 | Lenti ORF clone of Robo1 (Myc-DDK-tagged) - Mouse roundabout homolog 1 (Drosophila) (Robo1) |
CNY 16,060.00 |
|
MR224013L4 | Lenti ORF clone of Robo1 (mGFP-tagged) - Mouse roundabout homolog 1 (Drosophila) (Robo1) |
CNY 16,060.00 |