Med1 (NM_001080118) Mouse Untagged Clone
CAT#: MC224696
Med1 (untagged) - Mouse mediator complex subunit 1 (Med1), transcript variant 3, (10ug)
CNY 20,240.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | AI480703; CRSP210; DRIP205; l11Jus15; PBP; Pparbp; TRAP220 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC224696 representing NM_001080118
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGCTCAGGGGGAAACCGAGGACTCAGAGAGGCTGAGTAAGATGAGCTCCCTCCTGGAACGGCTCC ATGCAAAATTTAACCAGAACAGACCTTGGAGTGAAACCATTAAGCTTGTGCGTCAAGTAATGGAGAAGAG GGTCGTAATGAGTTCTGGAGGGCATCAGCATTTGGTCAGCTGTTTGGAGACATTGCAGAAGGCTCTCAAA GTAACATCTTTGCCAGCAATGACTGATCGTTTGGAATCTATAGCCAGACAGAATGGACTGGGCTCTCACC TCAGTGCCAGTGGCACTGAGTGTTACATCACGTCAGATATGTTCTATGTGGAAGTGCAGTTAGATCCTGC AGGACAGCTTTGTGATGTCAAAGTGGCTCACCATGGGGAGAATCCTGTGAGCTGTCCAGAGCTTGTACAG CAGTTAAGGGAAAAGAATTTTGAGGAATTTTCCAAGCATCTTAAGGGTCTTGTTAATCTGTATAATCTCC CAGGGGACAACAAACTGAAGACTAAAATGTATCTGGCTCTCCAATCCTTAGAACAGGACCTTTCTAAAAT GGCTATTATGTACTGGAAGGCAACCAACGCCGCTCCCTTGGATAAGATTCTTCATGGAAGTGTTGGTTAT CTCACCCCGCGGAGTGGGGGTCATTTAATGAATATGAAATACTATGCCTCTCCATCTGACCTGCTGGATG ATAAGACTGCCTCTCCTATCATTTTGCATGAAAAGAATGTTCCTCGGTCTTTGGGAATGAATGCCTCAGT GACAATTGAAGGAACCTCTGCTATGTACAAACTCCCAATTGCCCCATTAATTATGGGGTCACACCCAGCT GACAACAAATGGACCCCTTCTTTCTCCGCAGTCACTAGTGCCAACAGTGTTGATCTTCCTGCGTGTTTCT TCTTGAAATTTCCCCAGCCAATTCCAGTATCTAAAGCATTTGTTCAGAAACTGCAAAATTGCACAGGAAT CCCGTTGTTTGAGACTCCGCCCACTTACCTGCCCCTGTATGAACTCATCACTCAGTTTGAGCTGTCAAAG GATCCTGACCCTTTACCTTTGAATCACAACATGCGATTTTACGCTGCTCTTCCAGGTCAGCAGCACTGCT ATTTTCTCAATAAAGATGCTCCTCTTCCTGATGGTCAGAGCCTGCAGGGAACACTGGTCAGCAAAATCAC CTTCCAGCACCCTGGCCGAGTTCCTCTTATCTTGAATATGATCAGACACCAAGTGGCCTATAACACTCTA ATTGGAAGCTGTGTCAAAAGAACTATTTTAAAAGAAGATTCTCCTGGGCTCCTCCAGTTTGAAGTGTGTC CTCTCTCAGAATCTCGCTTCAGTGTATCTTTTCAGCACCCTGTGAATGACTCCCTTGTGTGTGTGGTGAT GGATGTGCAAGACTCAACACATGTGAGCTGTAAACTCTACAAGGGGCTGTCAGATGCACTAATCTGTACA GACGACTTCATTGCCAAAGTTGTTCAAAGATGTATGTCCATTCCTGTGACGATGAGGGCTATTCGGAGGA AGGCTGAAACCATACAGGCTGACACCCCAGCACTGTCTCTCATTGCAGAGACAGTTGAAGACATGGTGAA AAAGAACCTGCCCCCGGCTAGCAGCCCAGGGTATGGCATGACCACAGGCAACAACCCAATGAGTGGTACC ACTACACCAACCAACACCTTTCCGGGGGGTCCCATTACCACCTTGTTTAATATGAGCATGAGCATCAAAG ATCGGCATGAGTCGGTGGGCCATGGGGAGGACTTCAGCAAGGTGTCTCAGAACCCAATTCTTACCAGTTT GTTGCAAATCACAGGGAACGGGGGGTCTACCATTGGCTCGAGTCCGACCCCTCCTCATCACACGCCGCCA CCTGTCTCTTCGATGGCCGGCAACACCAAGAACCACCCGATGCTCATGAACCTTCTTAAAGATAACCCTG CCCAGGATTTCTCAACCCTTTATGGAAGCAGCCCTTTAGAAAGGCAGAACTCCTCTTCCGGATCACCCCG GATGGAAATGTGCTCGGGGAGCAACAAGGCCAAGAAGAAGAAGTCGTCAAGAGTCCCACCTGACAAACCC AAGCACCAGACTGAAGACGATTTCCAGAGGGAGCTCTTTTCCATGGATGTCGACTCACAGAACCCTATGT TTGACGTCAGCATGACCGCTGACGCGCTGGATACACCTCATATCACCCCAGCTCCAAGCCAGTGTAGCAC TCCCCCAGCAACGTACCCACAGCCAGTGTCTCACCCCCAGCCCAGTATTCAGAGGATGGTCCGACTGTCC AGTTCAGACAGCATTGGCCCAGATGTAACTGATATTCTTTCAGATATTGCCGAAGAAGCTTCAAAGCTTC CCAGCACGAGTGATGACTGCCCACCAATTGGCACCCCTGTTCGAGATTCCTCAAGTTCTGGGCATTCTCA GAGTGCCCTCTTTGATTCTGATGTCTTTCAAACTAATAATAATGAAAATCCATACACTGATCCAGCTGAC CTTATTGCAGATGCTGCTGGAAGCCCCAATAGTGATTCTCCTACCAATCATTTTTTCCCTGATGGAGTAG ATTTCAATCCTGATTTGTTGAACAGCCAAAGCCAAAGTGGTTTTGGAGAGGAGTATTTTGATGAAAGTAG TCAGAGTGGGGATAATGATGATTTCAAAGGATTTGCATCTCAGGCATTAAATACATTGGGGATGCCAATG CTTGGAGGTGACAATGGGGAGCCAAAATTTAAGGGCAGCAGCCAGGCTGACACGGTGGACTTCAGTATTA TATCAGTAGCCGGTAAGGCTTTGGGTGCTGCAGATCTGATGGAGCACCACAGTGGGAGTCAGAGTCCTTT ACTGACCACTGGAGAATTAGGGAAAGAAAAAACTCAAAAGAGGGTGAAGGAAGGCAACGGCACAGGTGCT AGCAGTGGATCAGGTCCAGGGTCAGACAGCAAGCCAGGCAAGCGCAGCCGCACTCCCTCCAATGATGGGA AGAGCAAGGATAAGCCTCCAAAGCGGAAGAAGGCAGACACTGAGGGGAAGTCCCCATCTCACAGTTCTTC TAATAGACCTTTCACCCCACCTACCAGCACGGGTGGGTCCAAATCCCCAGGCAGTTCAGGACGATCTCAG ACGCCCCCAGGTGTTGCCACCCCGCCCATTCCCAAGATTACCATTCAGATTCCTAAAGGGACAGTGATGG TGGGCAAGCCCTCCTCTCACAGTCAGTACACTAGCAGTGGTTCTGTGTCTTCCTCTGGCAGCAAAAGCCA CCATAGTCATTCTTCCTCCTCCTCTTCCTTAGCTTCTGCTTCCACCTCAGGCAAGGTGAAAAGCAGTAAA TCTGAAGGCTCATCAAGTTCCAAGCTCAGTGGCAGTATGTATGCTAGCCAAGGGTCTTCTGGATCCAGCC AGTCCAAAAATTCATCTCAGACTGGGGGGAAGCCAGGCTCCTCTCCCATTACCAAACATGGACTGAGCAG TGGGTCCAGCAGTACCAAGATGAAACCTCAAGGCAAGCCATCCTCCCTTATGAACCCTTCTATAAGTAAG CCAAACATATCCCCTTCCCATTCAAGGCCTCCCGGAGGCTCAGATAAGCTTGCCTCTCCAATGAAGCCTG TTCCTGGAACCCCCCCATCCTCTAAAGCCAAGTCCCCTATCAGTTCAGGTTCCAGTGGTTCTCATGTGTC AGGAACTAGTTCAAGCTCTGGTATGAAGTCATCTTCAGGGTCAGCATCCTCAGGCTCAGTGTCTCAAAAA ACCCCTCCAGCATCTAATTCTTGTACACCATCTTCCTCTTCGTTTTCCTCAAGTGGTTCTTCCATGTCAT CCTCTCAGAATCAACATGGCAGTTCCAAAGGGAAATCTCCCAGTAGGAATAAGAAGCCTTCCTTGACAGC TGTCATAGATAAATTGAAGCATGGGGTGGTTACCAGTGGGCCTGGGGGTGAGGATCCAATAGACAGTCAG ATGGGCGCAAGCACAAATTCTTCTAACCATCCCATGTCCTCCAAACATAACACGTCAGGAGGGGAGTTCC AGAGCAAACGTGAGAAAAGTGATAAAGACAAATCCAAGGTCTCTGCTTCTGGGGGGTCAGTGGATTCCTC TAAGAAGACTTCAGAGTCAAAAAATGTGGGGAGCACGGGGGTGGCAAAAATCATTATCAGCAAGCACGAC GGAGGCTCCCCGAGCATCAAAGCCAAAGTGACGCTACAGAAACCTGGAGAAAGTGGTGGAGATGGGCTCA GGCCACAGATAGCCTCATCAAAGAACTATGGCTCTCCACTTATCAGTGGTTCCACTCCAAAGCACGAACG GGGTTCTCCCAGCCACAGTAAGTCGCCAGCATATACACCACAGAATGTGGACAGTGAAAGTGAGTCAGGC TCCTCCATAGCAGAGAGATCCTACCAGAACAGTCCCAGCTCAGAGGATGGTATCCGACCACTTCCAGAGT ACAGCACTGAGAAGCATAAGAAGCACAAAAAGGAAAAGAAGAAAGTCAGAGACAAAGACAGAGACAAGAA GAAGTCTCACAGCATGAAGCCAGAGAACTGGTCGAAATCCCCCATTTCTTCAGATCCGACGGCGTCTGTG ACAAATAACCCTATCTTATCTGCAGACAGGCCTTCTAGGCTCAGCCCTGACTTCATGATTGGGGAGGAAG ATGATGATCTCATGGATGTGGCCCTGATTGGCAATTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001080118 |
Insert Size | 4728 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001080118.1, NP_001073587.1 |
RefSeq Size | 6518 bp |
RefSeq ORF | 4728 bp |
Locus ID | 19014 |
UniProt ID | Q925J9 |
Gene Summary | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Essential for embryogenesis, including development of the central nervous system, heart, liver and placenta and for erythropoiesis. Also required for normal transcriptional control of thyroid-stimulating hormone beta (TSHB) in the pituitary. Acts as a coactivator for GATA1-mediated transcriptional activation during erythroid differentiation of K562 erythroleukemia cells (By similarity).[UniProtKB/Swiss-Prot Function] |
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Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG222481 | Med1 (tGFP-tagged) - Mouse mediator complex subunit 1 (Med1) transcript variant 3, (10ug) |
CNY 17,290.00 |
|
MR222481 | Med1 (Myc-DDK-tagged) - Mouse mediator complex subunit 1 (Med1), transcript variant 3 |
CNY 15,680.00 |
|
MR222481L3 | Lenti ORF clone of Med1 (Myc-DDK-tagged) - Mouse mediator complex subunit 1 (Med1), transcript variant 3 |
CNY 17,580.00 |
|
MR222481L4 | Lenti ORF clone of Med1 (mGFP-tagged) - Mouse mediator complex subunit 1 (Med1), transcript variant 3 |
CNY 17,580.00 |