Fmn2 (NM_019445) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AU024104
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224693 representing NM_019445 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAACCAGGATGGGAAGCTGAAGAGAAGCGCAGGTGATGCCTCCCACGAAGGCGGCGGAGCCGAGG ATGCCGCGGGGCCCAGGGATGCGGAAATCACAAAGAAGGCGAGCGGGAGCAAAAAGGCGCTTGGCAAGCA CGGCAAGGGGGGAGGGGGCAGCGGGGAGACCAGCAAGAAGAAGAGCAAGTCCGATTCTAGAGCCTCGGTG TTTTCCAACCTGCGGATCAGAAAGAACCTGACCAAGGGGAAAGGTGCCTGCGACTCGCGAGAGGATGTGC TGGACTCACAGGCCCTGCCGATTGGGGAGCTGGACAGCGCTCACTCTATAGTCACCAAGACCCCGGACCT CAGCCTCTCCGCTGAGGAGACAGGCCTATCGGATACCGAGTGTGCTGACCCTTTCGAGGTGATCCATCCC GGTGCTTCTAGGCCTGCTGAGGCTGGGGTAGGGATCCAGGCGACCGCGGAGGATTTGGAAACTGCGGCTG GAGCGCAAGATGGACAAAGGACCAGTTCTGGTTCAGACACGGACATCTACAGCTTCCACTCCGCTACGGA GCAGGAGGATTTGCTCTCAGACATCCAGCAGGCAATTCGTCTGCAACAGCAGCAGCAGCAGAAGCTGCTG CTCCAGGACTCCGAGGAGCCTGCAGCGCCCCCCACTGCCATCTCCCCTCAGCCTGGGGCCTTTCTGGGCC TGGACCAGTTCTTGCTGGGACCTAGAAGCGAGGCTGAAAAGGACACAGTACAGGCACTACCGGTGAGACC TGACTTGCCTGAGACCACCAAGTCTCTGGTGCCTGAGCATCCTCCGTCCTCCGGAAGCCACTTGACCTCC GAGACACCAGGTTATGCGACCGCCCCCTCCGCAGTCACAGACTCTCTCTCATCACCAGCCTTCACCTTTC CGGAGGCTGGGCCAGGGGAGGGAGCCGCCGGAGTTCCCGTGGCTGGAACTGGGGACACAGATGAGGAGTG CGAGGAGGATGCTTTTGAGGATGCCCCCCGCGGCTCTCCAGGGGAGGAATGGGTCCCAGAGGTGGAAGAA GCCTCACAGAGGCTGGAGAAAGAGCCGGAGGAGGGCATGCGAGAATCCATTACCTCCGCAGTCGTTTCTT TGCCTGGAAGCCCTGCGCCCAGCCCACGCTGCTTCAAACCTTACCCACTCATCACCCCCTGCTACATCAA GACCACCACTCGGCAGCTCAGCTCTCCTAATCACTCCCCGTCCCAGTCCCCCAACCAGAGTCCTAGGATC AAAAAGCGGCCGGACCCTTCCGTGAGCCGGAGCTCCAGAACCGCCTTGGCCTCTGCTGCAGCCCCGGCAA AAAAGCACCGGTTGGAGGGTGGCCTCACGGGCGGCCTCAGCCGCTCAGCCGACTGGACCGAGGAGCTGGG CGTCCGTACGCCAGGGGCAGGAGGCTCCGTGCACCTGCTGGGGCGCGGGGCTACTGCGGATGACAGTGGT GGCGGGTCCCCTGTACTGGCCGCCAAGGCACCTGGGGCTCCAGCGACAGCTGATGGCTTTCAGAACGTGT TCACAGGGAGAACACTGCTGGAGAAGCTGTTTAGCCAGCAGGAGAACGGGCCTCCAGAGGAAGCAGAAAA ATTTTGCTCCCGGATCATCGCCATGGGTCTTCTACTTCCTTTCAGTGACTGCTTCAGGGAACCGTGTAAT CAGAACGCTGGGTCCAGTTCAGCTCCATTTGATCAAGATCAGCTTTACACCTGGGCTGCGGTTAGTCAAC CCACACATTCGATGGATTACAGCGAAGGGCAGTTTCCCAGGAGAGAACCATCTATGTGGCCATCATCCAA GCTTCCTGAGGAAGAGCCCAGTCCCAAGGACGTTGATACAGAACCTAAATCCTCTATTTTGGAAAGCCCG AAAAAATGCTCAAATGGTGTCCAGCAGGAAGTTTTCGATGTGAAGTCTGAAGGACAGGCAACTGTAATTC AGCAACTGGAACAGACCATCGAGGATCTACGCACAAAAATAGCTGAGCTAGAGAAGCAATATCCAGCCCT GGACTTGGAGGGACCCAGAGGCCTCTCAGGACTTGAGAATGGATTGACAGCCTCTGCAGACGTCAGTCTA GATGCTCTTGTATTGCATGGGAAGGTTGCACAGCCTCCAAGGACTCTTGAGGCAAAATCAATACAGACTT CCCCAACAGAAGAAGGTAGAATCCTGACATTGCCACCTCCAAAGGCACCACCAGAGGGTCTTCTGGGGTC CCCTGCAGCTGCAAGTGGAGAGTCAGCCCTTCTAACCTCACCCTCTGGACCTCAGACAAAATTCTGTTCA GAGATTTCTCTGATTGTGTCTCCAAGGAGAATATCAGTACAGCTGGATGCCCAACAGATACAGAGTGCAT CACAACTACCACCACCTCCTCCTCTCCTTGGGTCTGATAGTCAGGGACAGCCCTCCCAACCTTCTCTGCA TACTGAGTCAGAAACCAGCCATGAACATTCTGTTTCCTCCTCCTTTGGAAACAACTGTAATGTCCCACCC GCACCACCTCTGCCTTGTACAGAGTCCTCCAGTTTCATGCCTGGCCTGGGCATGGCAATTCCCCCACCTC CCTGTCTCTCTGACATAACAGTGCCTGCTCTGCCCAGTCCTACAGCCCCAGCCCTACAATTTAGCAATCT ACAGGGACCAGAAATGCTGCCAGCCCCTCCCCAACCTCCTCCTCTTCCTGGGCTAGGAGTACCCCCTCCT CCCCCTGCCCCTCCTCTCCCTGGAATGGGAATACCTCCCCCACCTCCTCTCCCTGGAATGGGAATACCTC CCCCACCTCCTCTCCCTGGAATGGGAATATCTCCCCTACCTCCTCTCCCTGGAATGGGAATACCTCCTCC CCCTCCTCTACCTGGAGTGGGCATACCCCCTCCTCCTCCTCTTCCTGGAGTGGGCATACCCCCTCCTCCT CCTCTTCCTGGAGTGGGAATACCCCCTCCTCCTCCTCTTCCTGGAGTGGGAATACCTCCTCCCCCTCCTT TACCTGGAGTGGGAATACCCCCTCCTCCTCCTCTTCCTGGAGTGGGCATACCCCCTCCTCCTCCTCTTCC TGGAGTGGGAATACCCCCACCCCCTCCACTTCCAGGAGTGGGAATACCCCCTCCCCCTCCTTTACCTGGA TCAGGCATACCCCCTCCTCCTGCTCTACCTGGAGTTGCTATTCCTCCACCACCTCCTCTACCAGGTATGG GGGTTCCACCTCCCGCCCCACCTCCCCCTGGGGCAGGCATCCCCCCACCCCCACTGTTGCCTGGCTCAGG TCCTCCACACTCCTCACAAGTTGGGAGTAGCACTTTACCAGCAGCACCTCAAGGGTGTGGATTTCTTTTT CCTCCATTGCCCACTGGCTTGTTTGGATTAGGGATGAATCAGGACAGAGTGGCTAGGAAGCAGCTGATCG AGCCTTGCCGGCCAATGAAGCCTCTCTATTGGACAAGAATTCAACTCCATAGTAAAAGAGACTCCAGCCC TTCGCTTATTTGGGAAAAGATTGAAGAGCCATCCATAGACTGTCATGAATTTGAAGAATTATTTTCTAAA ACTGCAGTAAAGGAGAGAAAGAAACCTATTTCTGACACAATTTCAAAGACAAAGGCCAAACAAGTTGTCA AGTTGCTTAGCAACAAAAGGTCACAAGCAGTAGGAATTCTAATGTCTAGTCTGCATTTAGATATGAAAGA CATACAACATGCTGTTGTGAACTTGGACAACTCTGTGGTGGACCTGGAGACCCTTCAAGCTCTCTATGAG AATAGAGCACAGTCAGATGAACTGGAAAAAATTGAAAAGCACAGTCGATCTTCCAAAGACAAGGAAAACG CCAAGTCTCTCGACAAACCTGAACAGTTCCTGTATGAGCTGTCGCTAATCCCCAACTTCTCCGAGCGGGT CTTCTGTATCCTGTTTCAGTCCACATTTTCAGAGAGCATTTGCTCAATTCGTCGCAAGTTAGAATTGCTA CAAAAACTGTGTGAGACCTTAAAAAATGGACCAGGGGTCATGCAAGTCCTGGGTTTAGTTCTAGCCTTTG GGAACTACATGAATGCTGGGAACAAGACCCGAGGACAGGCAGATGGCTTTGGACTAGACATTTTGCCCAA GCTGAAGGATGTCAAAAGCAGCGACAACAGCAGAAGCCTTTTGTCATATATTGTTTCATATTATCTTCGA AATTTTGATGAGGATGCTGGCAAAGAGCAGTGTGTCTTCCCGCTGGCAGAACCCCAGGAGCTTTTCCAGG CCTCACAGATGAAGTTTGAAGACTTCCAGAAAGACCTCAGAAAACTAAAGAAAGACCTGAAAGCCTGTGA GGCGGAAGCCGGTAAAGTGTACCAGGTGTCCTCTGCGGAGCATATGCAGCCTTTCAAGGAAAACATGGAG CAGTTTATCAGTCAAGCTAAAATTGACCAAGAGTCACAGGAGGCTGCCCTGACAGAGACTCATAAATGCT TTTTGGAGACCACAGCCTACTACTTCATGAAACCAAAACTCGGCGAGAAGGAGGTGTCCCCAAATGTTTT CTTCAGTGTCTGGCATGAATTCAGCTCTGACTTTAAAGACGCTTGGAAAAAAGAGAACAAACTGATTCTG CAAGAGAGAGTCAAAGAAGCCGAGGAGGTGTGTAGGCAGAAGAAAGGAAAATCACTCTATAAAGTAAAAC CGAGACATGACTCTGGGATTAAAGCGAAGATAAGCATGAAAACGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_019445
Insert Size	4737 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_019445.2, NP_062318.2
RefSeq Size	6461 bp
RefSeq ORF	4737 bp
Locus ID	54418
UniProt ID	Q9JL04
Gene Summary	Actin-binding protein that is involved in actin cytoskeleton assembly and reorganization (PubMed:18848445, PubMed:21620703). Acts as an actin nucleation factor and promotes assembly of actin filaments together with SPIRE1 and SPIRE2 (PubMed:18848445, PubMed:21620703). Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport (PubMed:21983562). Required for asymmetric spindle positioning, asymmetric oocyte division and polar body extrusion during female germ cell meiosis (PubMed:12447394, PubMed:18848445, PubMed:19062278, PubMed:21620703). Plays a role in responses to DNA damage, cellular stress and hypoxia by protecting CDKN1A against degradation, and thereby plays a role in stress-induced cell cycle arrest (By similarity). Also acts in the nucleus: together with SPIRE1 and SPIRE2, promotes assembly of nuclear actin filaments in response to DNA damage in order to facilitate movement of chromatin and repair factors after DNA damage (By similarity). Protects cells against apoptosis by protecting CDKN1A against degradation (By similarity).[UniProtKB/Swiss-Prot Function]

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