Fmn2 (NM_019445) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AU024104
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR212040 representing NM_019445, codon optimized. Due to the complexity of NM_019445, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTAATCAGGATGGGAAACTGAAGCGCTCTGCTGGCGACGCATCACACGAAGGCGGTGGAGCCGAGG ATGCTGCAGGCCCGAGGGACGCCGAGATCACTAAGAAGGCTAGCGGCTCAAAAAAAGCCCTTGGCAAACA CGGTAAGGGTGGCGGGGGGAGTGGGGAGACATCTAAGAAGAAGAGCAAGTCTGACTCCCGAGCTTCTGTC TTTTCCAATTTGCGAATTCGGAAAAACCTGACAAAGGGTAAAGGCGCTTGTGATAGCAGGGAGGACGTGC TCGACAGCCAGGCCCTGCCCATTGGAGAGCTGGACTCAGCACACTCAATCGTAACTAAAACACCTGACCT TAGCCTGTCAGCCGAAGAAACAGGCCTGAGCGATACCGAGTGCGCTGACCCGTTTGAGGTCATCCACCCC GGCGCCTCTCGACCAGCGGAAGCCGGAGTCGGGATACAAGCGACCGCGGAGGACCTCGAAACCGCTGCAG GCGCCCAGGACGGCCAGCGCACCAGCTCTGGTAGCGATACCGATATCTATTCCTTTCATTCAGCCACCGA GCAGGAGGACCTGCTGTCTGACATTCAGCAGGCCATTCGGCTTCAGCAGCAGCAGCAGCAAAAGCTGCTT CTTCAAGATTCTGAAGAGCCCGCTGCCCCCCCCACTGCCATAAGCCCCCAACCTGGGGCATTTCTGGGTC TGGATCAGTTTTTGCTTGGCCCTCGCTCCGAAGCGGAGAAGGACACCGTGCAGGCACTCCCCGTCAGACC AGATCTGCCAGAGACTACTAAGTCTCTTGTCCCTGAGCATCCACCTTCCAGCGGATCACATCTGACATCA GAGACTCCAGGCTACGCAACAGCTCCCAGTGCCGTTACTGATAGTCTGTCCTCCCCAGCATTCACCTTTC CTGAGGCCGGCCCAGGGGAAGGTGCCGCAGGTGTCCCCGTTGCCGGCACCGGTGATACAGATGAAGAATG TGAAGAGGACGCATTCGAGGATGCACCAAGGGGTTCCCCCGGCGAGGAATGGGTCCCCGAGGTGGAGGAA GCTTCACAACGGTTGGAGAAGGAACCCGAGGAGGGCATGCGCGAGAGCATTACTAGTGCTGTGGTGTCCC TCCCCGGGAGCCCTGCCCCTTCACCACGCTGCTTTAAACCGTACCCACTCATTACGCCGTGCTATATTAA GACAACTACACGCCAGTTGAGCTCTCCAAACCACAGTCCTAGCCAGTCACCAAATCAGTCCCCACGAATC AAGAAGCGACCTGATCCATCCGTGTCACGGTCTAGTCGGACCGCACTGGCATCCGCTGCGGCACCAGCAA AGAAACACCGCCTCGAAGGCGGTCTGACTGGTGGCCTCTCTAGAAGTGCAGACTGGACTGAAGAGCTGGG GGTGAGGACCCCAGGGGCCGGCGGCTCCGTCCATCTCTTGGGTCGGGGGGCAACCGCTGACGATAGTGGC GGCGGATCTCCCGTTCTGGCCGCAAAGGCGCCCGGGGCTCCAGCCACGGCTGACGGCTTCCAGAACGTGT TCACCGGTAGGACTCTGCTGGAGAAGCTGTTCAGCCAACAGGAGAACGGCCCACCTGAGGAGGCAGAGAA ATTTTGCTCACGGATTATTGCTATGGGCCTGCTGCTTCCTTTTTCCGACTGTTTTAGGGAGCCCTGTAAT CAGAATGCAGGCTCCTCTAGCGCACCGTTCGACCAGGATCAACTTTATACTTGGGCCGCCGTCTCTCAGC CAACCCACTCTATGGATTATTCCGAGGGACAGTTCCCCCGCCGGGAACCGTCAATGTGGCCCTCTAGCAA ATTGCCCGAAGAGGAGCCATCTCCCAAGGACGTAGACACGGAACCAAAGAGCTCTATCCTCGAATCCCCC AAGAAATGTAGCAACGGCGTGCAGCAGGAAGTGTTCGACGTTAAGTCCGAGGGCCAGGCTACCGTGATCC AGCAACTGGAGCAGACAATCGAGGACCTTCGCACCAAGATCGCTGAGCTGGAAAAACAGTATCCTGCCCT GGATCTGGAGGGGCCCCGGGGTCTTAGCGGCCTGGAGAACGGGCTCACAGCCTCCGCTGACGTGTCTCTG GATGCATTGGTGCTGCACGGGAAGGTCGCACAGCCTCCTAGAACCCTTGAAGCGAAATCTATCCAGACTT CTCCTACAGAGGAGGGGCGAATTCTTACACTTCCACCACCTAAAGCTCCTCCAGAAGGACTGCTGGGGTC ACCCGCGGCAGCCTCAGGCGAATCCGCGCTTCTGACGTCTCCCTCAGGGCCTCAAACTAAGTTCTGTTCC GAGATATCCCTGATAGTGAGCCCCAGGAGGATCTCAGTGCAGCTGGATGCACAGCAAATTCAATCAGCTT CCCAGCTGCCACCCCCGCCTCCTCTTCTGGGATCAGACTCCCAGGGTCAGCCATCACAGCCCAGTTTGCA TACGGAAAGCGAAACATCCCACGAGCATAGCGTATCCTCCAGCTTTGGGAACAACTGTAATGTGCCACCG GCCCCCCCACTGCCTTGTACAGAATCATCTAGTTTTATGCCCGGGCTCGGAATGGCAATTCCACCTCCGC CCTGCCTCTCCGACATAACCGTGCCTGCACTGCCTTCACCTACAGCCCCCGCTCTCCAGTTTTCAAATCT GCAGGGCCCTGAAATGCTGCCAGCGCCACCGCAGCCCCCCCCATTGCCAGGCCTGGGAGTACCACCACCA CCCCCGGCCCCACCCCTTCCAGGCATGGGTATTCCGCCTCCTCCACCCCTTCCGGGAATGGGCATCCCAC CTCCACCTCCTCTGCCAGGTATGGGAATTTCACCCTTGCCACCTCTACCTGGAATGGGAATCCCTCCACC TCCCCCACTGCCTGGAGTCGGTATTCCACCTCCACCGCCTCTTCCAGGAGTGGGAATCCCACCACCTCCA CCACTGCCTGGAGTGGGAATACCTCCACCTCCACCTCTGCCAGGTGTTGGCATCCCTCCACCGCCACCTC TGCCTGGAGTTGGCATTCCACCACCTCCGCCACTTCCAGGCGTAGGCATCCCTCCACCACCTCCGTTGCC TGGTGTGGGTATCCCACCACCTCCTCCACTCCCAGGTGTGGGTATACCACCACCGCCTCCTCTGCCAGGC AGCGGAATCCCTCCTCCTCCAGCTCTGCCAGGTGTTGCTATACCTCCACCACCTCCACTCCCCGGCATGG GTGTGCCTCCGCCAGCTCCTCCACCTCCTGGCGCTGGAATTCCACCCCCGCCCCTCTTGCCAGGCTCTGG GCCTCCCCATAGCAGTCAGGTCGGATCAAGCACTCTCCCAGCTGCCCCACAAGGATGCGGGTTTTTGTTC CCCCCACTGCCTACTGGGTTGTTCGGACTCGGCATGAACCAAGACAGGGTGGCGAGAAAGCAACTGATCG AACCATGTAGACCCATGAAGCCGCTGTATTGGACTCGCATTCAGCTGCACTCAAAGCGGGATTCTAGCCC CAGCCTGATTTGGGAAAAAATCGAGGAGCCCTCCATCGACTGTCACGAATTCGAAGAGCTGTTTTCAAAA ACCGCCGTTAAAGAAAGAAAGAAGCCCATCAGCGATACCATCTCTAAGACAAAGGCTAAGCAGGTCGTGA AGTTGTTGTCAAATAAGCGCTCCCAAGCCGTGGGGATCCTTATGAGCAGCCTGCATCTCGACATGAAAGA TATTCAGCACGCAGTAGTAAACCTGGACAACAGCGTCGTCGACTTGGAAACTCTTCAGGCCCTCTACGAG AACCGGGCCCAATCCGACGAACTGGAGAAAATCGAGAAGCACAGTAGGAGCAGCAAAGACAAGGAGAACG CAAAGAGTCTGGACAAACCTGAGCAATTCCTGTATGAGTTGAGTCTTATCCCAAACTTCTCTGAGCGGGT ATTCTGCATTCTTTTCCAGAGCACCTTCTCAGAATCTATCTGCTCTATAAGACGCAAACTGGAGCTCCTG CAGAAGCTCTGCGAAACTCTGAAGAACGGACCGGGGGTTATGCAGGTGCTTGGGCTTGTTCTGGCATTCG GCAATTATATGAACGCCGGCAACAAGACCAGAGGACAAGCTGATGGATTTGGTTTGGACATTCTTCCAAA ACTTAAAGACGTGAAGAGCTCAGATAACTCCAGATCCCTTCTGTCCTACATTGTCTCATATTACCTGAGA AACTTTGATGAAGATGCCGGCAAGGAACAGTGCGTGTTTCCTCTCGCCGAGCCACAGGAACTGTTCCAGG CCTCACAGATGAAGTTCGAGGATTTCCAGAAGGACCTGCGAAAGCTGAAGAAGGACTTGAAGGCGTGTGA GGCTGAGGCCGGTAAGGTGTACCAGGTCAGTAGTGCCGAGCACATGCAACCCTTCAAAGAGAACATGGAG CAGTTCATTTCCCAGGCAAAAATTGATCAAGAAAGCCAGGAGGCTGCCCTCACTGAGACTCACAAATGTT TTCTTGAAACAACCGCATACTACTTTATGAAGCCAAAATTGGGAGAGAAGGAAGTTAGCCCGAATGTCTT TTTCTCCGTCTGGCATGAGTTCTCATCCGACTTCAAAGACGCTTGGAAGAAGGAGAATAAACTGATCCTG CAAGAACGGGTCAAAGAGGCTGAAGAAGTGTGTCGCCAAAAGAAGGGGAAAAGCCTCTATAAAGTGAAGC CACGACACGATAGCGGCATTAAGGCTAAAATCAGTATGAAGACT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR212040 representing NM_019445 Red=Cloning site Green=Tags(s) MGNQDGKLKRSAGDASHEGGGAEDAAGPRDAEITKKASGSKKALGKHGKGGGGSGETSKKKSKSDSRASV FSNLRIRKNLTKGKGACDSREDVLDSQALPIGELDSAHSIVTKTPDLSLSAEETGLSDTECADPFEVIHP GASRPAEAGVGIQATAEDLETAAGAQDGQRTSSGSDTDIYSFHSATEQEDLLSDIQQAIRLQQQQQQKLL LQDSEEPAAPPTAISPQPGAFLGLDQFLLGPRSEAEKDTVQALPVRPDLPETTKSLVPEHPPSSGSHLTS ETPGYATAPSAVTDSLSSPAFTFPEAGPGEGAAGVPVAGTGDTDEECEEDAFEDAPRGSPGEEWVPEVEE ASQRLEKEPEEGMRESITSAVVSLPGSPAPSPRCFKPYPLITPCYIKTTTRQLSSPNHSPSQSPNQSPRI KKRPDPSVSRSSRTALASAAAPAKKHRLEGGLTGGLSRSADWTEELGVRTPGAGGSVHLLGRGATADDSG GGSPVLAAKAPGAPATADGFQNVFTGRTLLEKLFSQQENGPPEEAEKFCSRIIAMGLLLPFSDCFREPCN QNAGSSSAPFDQDQLYTWAAVSQPTHSMDYSEGQFPRREPSMWPSSKLPEEEPSPKDVDTEPKSSILESP KKCSNGVQQEVFDVKSEGQATVIQQLEQTIEDLRTKIAELEKQYPALDLEGPRGLSGLENGLTASADVSL DALVLHGKVAQPPRTLEAKSIQTSPTEEGRILTLPPPKAPPEGLLGSPAAASGESALLTSPSGPQTKFCS EISLIVSPRRISVQLDAQQIQSASQLPPPPPLLGSDSQGQPSQPSLHTESETSHEHSVSSSFGNNCNVPP APPLPCTESSSFMPGLGMAIPPPPCLSDITVPALPSPTAPALQFSNLQGPEMLPAPPQPPPLPGLGVPPP PPAPPLPGMGIPPPPPLPGMGIPPPPPLPGMGISPLPPLPGMGIPPPPPLPGVGIPPPPPLPGVGIPPPP PLPGVGIPPPPPLPGVGIPPPPPLPGVGIPPPPPLPGVGIPPPPPLPGVGIPPPPPLPGVGIPPPPPLPG SGIPPPPALPGVAIPPPPPLPGMGVPPPAPPPPGAGIPPPPLLPGSGPPHSSQVGSSTLPAAPQGCGFLF PPLPTGLFGLGMNQDRVARKQLIEPCRPMKPLYWTRIQLHSKRDSSPSLIWEKIEEPSIDCHEFEELFSK TAVKERKKPISDTISKTKAKQVVKLLSNKRSQAVGILMSSLHLDMKDIQHAVVNLDNSVVDLETLQALYE NRAQSDELEKIEKHSRSSKDKENAKSLDKPEQFLYELSLIPNFSERVFCILFQSTFSESICSIRRKLELL QKLCETLKNGPGVMQVLGLVLAFGNYMNAGNKTRGQADGFGLDILPKLKDVKSSDNSRSLLSYIVSYYLR NFDEDAGKEQCVFPLAEPQELFQASQMKFEDFQKDLRKLKKDLKACEAEAGKVYQVSSAEHMQPFKENME QFISQAKIDQESQEAALTETHKCFLETTAYYFMKPKLGEKEVSPNVFFSVWHEFSSDFKDAWKKENKLIL QERVKEAEEVCRQKKGKSLYKVKPRHDSGIKAKISMKT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_019445
ORF Size	4734 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_019445.2, NP_062318.2
RefSeq Size	6461 bp
RefSeq ORF	4737 bp
Locus ID	54418
UniProt ID	Q9JL04
MW	167.4 kDa
Gene Summary	Actin-binding protein that is involved in actin cytoskeleton assembly and reorganization (PubMed:18848445, PubMed:21620703). Acts as an actin nucleation factor and promotes assembly of actin filaments together with SPIRE1 and SPIRE2 (PubMed:18848445, PubMed:21620703). Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport (PubMed:21983562). Required for asymmetric spindle positioning, asymmetric oocyte division and polar body extrusion during female germ cell meiosis (PubMed:12447394, PubMed:18848445, PubMed:19062278, PubMed:21620703). Plays a role in responses to DNA damage, cellular stress and hypoxia by protecting CDKN1A against degradation, and thereby plays a role in stress-induced cell cycle arrest (By similarity). Also acts in the nucleus: together with SPIRE1 and SPIRE2, promotes assembly of nuclear actin filaments in response to DNA damage in order to facilitate movement of chromatin and repair factors after DNA damage (By similarity). Protects cells against apoptosis by protecting CDKN1A against degradation (By similarity).[UniProtKB/Swiss-Prot Function]

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