Med1 (NM_013634) Mouse Untagged Clone

CAT#: MC224683

Med1 (untagged) - Mouse mediator complex subunit 1 (Med1), transcript variant 1, (10ug)



  "NM_013634" in other vectors (4)

CNY 20,050.00


货期*
8周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AI480703; CRSP210; DRIP205; l11Jus15; PBP; Pparbp; TRAP220
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC224683 representing NM_013634
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCTCCCTCCTGGAACGGCTCCATGCAAAATTTAACCAGAACAGACCTTGGAGTGAAACCATTAAGC
TTGTGCGTCAAGTAATGGAGAAGAGGGTCGTAATGAGTTCTGGAGGGCATCAGCATTTGGTCAGCTGTTT
GGAGACATTGCAGAAGGCTCTCAAAGTAACATCTTTGCCAGCAATGACTGATCGTTTGGAATCTATAGCC
AGACAGAATGGACTGGGCTCTCACCTCAGTGCCAGTGGCACTGAGTGTTACATCACGTCAGATATGTTCT
ATGTGGAAGTGCAGTTAGATCCTGCAGGACAGCTTTGTGATGTCAAAGTGGCTCACCATGGGGAGAATCC
TGTGAGCTGTCCAGAGCTTGTACAGCAGTTAAGGGAAAAGAATTTTGAGGAATTTTCCAAGCATCTTAAG
GGTCTTGTTAATCTGTATAATCTCCCAGGGGACAACAAACTGAAGACTAAAATGTATCTGGCTCTCCAAT
CCTTAGAACAGGACCTTTCTAAAATGGCTATTATGTACTGGAAGGCAACCAACGCCGCTCCCTTGGATAA
GATTCTTCATGGAAGTGTTGGTTATCTCACCCCGCGGAGTGGGGGTCATTTAATGAATATGAAATACTAT
GCCTCTCCATCTGACCTGCTGGATGATAAGACTGCCTCTCCTATCATTTTGCATGAAAAGAATGTTCCTC
GGTCTTTGGGAATGAATGCCTCAGTGACAATTGAAGGAACCTCTGCTATGTACAAACTCCCAATTGCCCC
ATTAATTATGGGGTCACACCCAGCTGACAACAAATGGACCCCTTCTTTCTCCGCAGTCACTAGTGCCAAC
AGTGTTGATCTTCCTGCGTGTTTCTTCTTGAAATTTCCCCAGCCAATTCCAGTATCTAAAGCATTTGTTC
AGAAACTGCAAAATTGCACAGGAATCCCGTTGTTTGAGACTCCGCCCACTTACCTGCCCCTGTATGAACT
CATCACTCAGTTTGAGCTGTCAAAGGATCCTGACCCTTTACCTTTGAATCACAACATGCGATTTTACGCT
GCTCTTCCAGGTCAGCAGCACTGCTATTTTCTCAATAAAGATGCTCCTCTTCCTGATGGTCAGAGCCTGC
AGGGAACACTGGTCAGCAAAATCACCTTCCAGCACCCTGGCCGAGTTCCTCTTATCTTGAATATGATCAG
ACACCAAGTGGCCTATAACACTCTAATTGGAAGCTGTGTCAAAAGAACTATTTTAAAAGAAGATTCTCCT
GGGCTCCTCCAGTTTGAAGTGTGTCCTCTCTCAGAATCTCGCTTCAGTGTATCTTTTCAGCACCCTGTGA
ATGACTCCCTTGTGTGTGTGGTGATGGATGTGCAAGACTCAACACATGTGAGCTGTAAACTCTACAAGGG
GCTGTCAGATGCACTAATCTGTACAGACGACTTCATTGCCAAAGTTGTTCAAAGATGTATGTCCATTCCT
GTGACGATGAGGGCTATTCGGAGGAAGGCTGAAACCATACAGGCTGACACCCCAGCACTGTCTCTCATTG
CAGAGACAGTTGAAGACATGGTGAAAAAGAACCTGCCCCCGGCTAGCAGCCCAGGGTATGGCATGACCAC
AGGCAACAACCCAATGAGTGGTACCACTACACCAACCAACACCTTTCCGGGGGGTCCCATTACCACCTTG
TTTAATATGAGCATGAGCATCAAAGATCGGCATGAGTCGGTGGGCCATGGGGAGGACTTCAGCAAGGTGT
CTCAGAACCCAATTCTTACCAGTTTGTTGCAAATCACAGGGAACGGGGGGTCTACCATTGGCTCGAGTCC
GACCCCTCCTCATCACACGCCGCCACCTGTCTCTTCGATGGCCGGCAACACCAAGAACCACCCGATGCTC
ATGAACCTTCTTAAAGATAACCCTGCCCAGGATTTCTCAACCCTTTATGGAAGCAGCCCTTTAGAAAGGC
AGAACTCCTCTTCCGGATCACCCCGGATGGAAATGTGCTCGGGGAGCAACAAGGCCAAGAAGAAGAAGTC
GTCAAGAGTCCCACCTGACAAACCCAAGCACCAGACTGAAGACGATTTCCAGAGGGAGCTCTTTTCCATG
GATGTCGACTCACAGAACCCTATGTTTGACGTCAGCATGACCGCTGACGCGCTGGATACACCTCATATCA
CCCCAGCTCCAAGCCAGTGTAGCACTCCCCCAGCAACGTACCCACAGCCAGTGTCTCACCCCCAGCCCAG
TATTCAGAGGATGGTCCGACTGTCCAGTTCAGACAGCATTGGCCCAGATGTAACTGATATTCTTTCAGAT
ATTGCCGAAGAAGCTTCAAAGCTTCCCAGCACGAGTGATGACTGCCCACCAATTGGCACCCCTGTTCGAG
ATTCCTCAAGTTCTGGGCATTCTCAGAGTGCCCTCTTTGATTCTGATGTCTTTCAAACTAATAATAATGA
AAATCCATACACTGATCCAGCTGACCTTATTGCAGATGCTGCTGGAAGCCCCAATAGTGATTCTCCTACC
AATCATTTTTTCCCTGATGGAGTAGATTTCAATCCTGATTTGTTGAACAGCCAAAGCCAAAGTGGTTTTG
GAGAGGAGTATTTTGATGAAAGTAGTCAGAGTGGGGATAATGATGATTTCAAAGGATTTGCATCTCAGGC
ATTAAATACATTGGGGATGCCAATGCTTGGAGGTGACAATGGGGAGCCAAAATTTAAGGGCAGCAGCCAG
GCTGACACGGTGGACTTCAGTATTATATCAGTAGCCGGTAAGGCTTTGGGTGCTGCAGATCTGATGGAGC
ACCACAGTGGGAGTCAGAGTCCTTTACTGACCACTGGAGAATTAGGGAAAGAAAAAACTCAAAAGAGGGT
GAAGGAAGGCAACGGCACAGGTGCTAGCAGTGGATCAGGTCCAGGGTCAGACAGCAAGCCAGGCAAGCGC
AGCCGCACTCCCTCCAATGATGGGAAGAGCAAGGATAAGCCTCCAAAGCGGAAGAAGGCAGACACTGAGG
GGAAGTCCCCATCTCACAGTTCTTCTAATAGACCTTTCACCCCACCTACCAGCACGGGTGGGTCCAAATC
CCCAGGCAGTTCAGGACGATCTCAGACGCCCCCAGGTGTTGCCACCCCGCCCATTCCCAAGATTACCATT
CAGATTCCTAAAGGGACAGTGATGGTGGGCAAGCCCTCCTCTCACAGTCAGTACACTAGCAGTGGTTCTG
TGTCTTCCTCTGGCAGCAAAAGCCACCATAGTCATTCTTCCTCCTCCTCTTCCTTAGCTTCTGCTTCCAC
CTCAGGCAAGGTGAAAAGCAGTAAATCTGAAGGCTCATCAAGTTCCAAGCTCAGTGGCAGTATGTATGCT
AGCCAAGGGTCTTCTGGATCCAGCCAGTCCAAAAATTCATCTCAGACTGGGGGGAAGCCAGGCTCCTCTC
CCATTACCAAACATGGACTGAGCAGTGGGTCCAGCAGTACCAAGATGAAACCTCAAGGCAAGCCATCCTC
CCTTATGAACCCTTCTATAAGTAAGCCAAACATATCCCCTTCCCATTCAAGGCCTCCCGGAGGCTCAGAT
AAGCTTGCCTCTCCAATGAAGCCTGTTCCTGGAACCCCCCCATCCTCTAAAGCCAAGTCCCCTATCAGTT
CAGGTTCCAGTGGTTCTCATGTGTCAGGAACTAGTTCAAGCTCTGGTATGAAGTCATCTTCAGGGTCAGC
ATCCTCAGGCTCAGTGTCTCAAAAAACCCCTCCAGCATCTAATTCTTGTACACCATCTTCCTCTTCGTTT
TCCTCAAGTGGTTCTTCCATGTCATCCTCTCAGAATCAACATGGCAGTTCCAAAGGGAAATCTCCCAGTA
GGAATAAGAAGCCTTCCTTGACAGCTGTCATAGATAAATTGAAGCATGGGGTGGTTACCAGTGGGCCTGG
GGGTGAGGATCCAATAGACAGTCAGATGGGCGCAAGCACAAATTCTTCTAACCATCCCATGTCCTCCAAA
CATAACACGTCAGGAGGGGAGTTCCAGAGCAAACGTGAGAAAAGTGATAAAGACAAATCCAAGGTCTCTG
CTTCTGGGGGGTCAGTGGATTCCTCTAAGAAGACTTCAGAGTCAAAAAATGTGGGGAGCACGGGGGTGGC
AAAAATCATTATCAGCAAGCACGACGGAGGCTCCCCGAGCATCAAAGCCAAAGTGACGCTACAGAAACCT
GGAGAAAGTGGTGGAGATGGGCTCAGGCCACAGATAGCCTCATCAAAGAACTATGGCTCTCCACTTATCA
GTGGTTCCACTCCAAAGCACGAACGGGGTTCTCCCAGCCACAGTAAGTCGCCAGCATATACACCACAGAA
TGTGGACAGTGAAAGTGAGTCAGGCTCCTCCATAGCAGAGAGATCCTACCAGAACAGTCCCAGCTCAGAG
GATGGTATCCGACCACTTCCAGAGTACAGCACTGAGAAGCATAAGAAGCACAAAAAGGAAAAGAAGAAAG
TCAGAGACAAAGACAGAGACAAGAAGAAGTCTCACAGCATGAAGCCAGAGAACTGGTCGAAATCCCCCAT
TTCTTCAGATCCGACGGCGTCTGTGACAAATAACCCTATCTTATCTGCAGACAGGCCTTCTAGGCTCAGC
CCTGACTTCATGATTGGGGAGGAAGATGATGATCTCATGGATGTGGCCCTGATTGGCAATTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_013634
Insert Size 4683 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_013634.2, NP_038662.2
RefSeq Size 6383 bp
RefSeq ORF 4683 bp
Locus ID 19014
UniProt ID Q925J9
Gene Summary Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Essential for embryogenesis, including development of the central nervous system, heart, liver and placenta and for erythropoiesis. Also required for normal transcriptional control of thyroid-stimulating hormone beta (TSHB) in the pituitary. Acts as a coactivator for GATA1-mediated transcriptional activation during erythroid differentiation of K562 erythroleukemia cells (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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