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Arhgef12 (NM_027144) Mouse Untagged Clone
CAT＃: MC224664
Arhgef12 (untagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), (10ug)
CNY 19,860.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2310014B11Rik; AU019857; Larg; mKIAA0382 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC224664 representing NM_027144
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGGCACACAGTCTACTATCACGGACAGGTTTCCCCTCAAAAAACCTATAAGGCATGGAAGTATTT TGAACCGAGAGTCACCAACAGATAAGAAACAGAAAGTTGAACGCAGTTCATCGCATGATTTTGACCCCAC AGATAGCTCCTCCAAGAAGACAAAGTCTAGTTCAGAGGAGAGTAGATCCGAGATCTATGGTCTTGTTCAG CGCTGTGTGATCATCCAGAAAGACGACAATGGCTTCGGGCTGACAGTCAGTGGAGACAATCCGGTCTTCG TACAATCTGTCAAAGAAGATGGAGCAGCCATGCGTGCTGGAGTACAAACAGGTGATCGAATCATCAAGGT GAATGGTACTCTGGTGACTCATTCAAATCATCTGGAGGTGGTGAAACTAATCAGATCTGGTTCCTATGTA GCTCTCACTGTCCAGGGACGCCCACCTGGGTCGCCCCAGATCCCACTTGCTGATTCTGAAGTAGAGCCAT CAGTCACTGGACATATGTCTCCCATTATGACATCCCCTCATTCCCCTGGAGCAGCCGGGAATATGGAGAG AATCACCAGTCCTGTGCTCGTGGGGGAGGAAAACAATGTGGTCCATAACCAGAAAGTAGAGATTCTGAGA AAGATGTTACAGAAGGAACAGGAACGGCTGCAGTTGCTGCAGGAAGATTACAACCGAACGGCTACCCAAA GATTGCTCAAGGAGATCCAGGAGGCCAAGAAGCACATCCCGCAGCTGCAGGAGCAGCTGTCCAAAGCCAC GGGCTCTGCTCAGGACGGAGCTGTAATTGCACCCTCCAGGCCATTAGGTGATGCCCTAACACTCAGTGAG GCAGAAGCAGATCCTGGAGATGGCCTGTGCAGGACTGACTGGAGCAGTGGAGATGCTTCTCGGCCCAGTA GTGACAGTGCTGATAGTCCCAAGAGTAGCCTGAGAGAAAGGAGTTACCTGGAGGAAGCCCCAGAGAGAAG TGAAGGAGTTCAGGACGCTCAGGAGCCGCAGTCGCTGGTCGGCAGTCCCTCCACCCGGGGCGCCCCTCAC ATCATTGGAGCAGAGGATGATGATTTTGGTACTGAGCATGAGCAGATCAATGGACAGTGCAGCTGTTTCC AGAGCATTGAATTGCTGAAGTCTCGCCCTGCTCACTTGGCTGTTTTCTTACACCATGTAGTCTCACAGTT TGACCCTGCAACATTGCTCTGTTATCTCTATTCAGACCTGTATAAGCAGACAAACTCCAAGGAGACTCGG CGTGTCTTCCTGGAGTTTCATCAGTTCTTCTTGGACCGGTCTGCACACCTGAAGGTTCCAGTTCCTGAGG AAATCTCCGTGGATCTAGAAAAGAGAAGACCTGAGCTTATTCCTGAGGATCTGCATCGCCTCTACATTCA GACAATGCAGGAGAGAGTCCACCCTGAAGTTCAGAGGCACTTGGAGGATTTCCGACAGAAACGCAGCATG GGGCTGACCTTGGCTGAGAGTGAGCTGACTAAACTTGATGCAGAGAGAGACAAGGACCGGGGAACTCTGG AGAAGGAGCGAGCATGTGCGGAACAGATCGTTACCAAAATTGAAGAAGTGTTGATGACTGCTCAGGCTGT AGAAGAAGAAAGGAGTTCCACAATGCAGTATGTAATTCTCATGTATATGAAGTATTTGGGAGTAAAAGTG AAAGAACCTCGAAACTTGGAGCACAAGCGGGGTCGGATCGGCTTCCTTCCAAAGATCAAGCAAAGCATGA AGAAAGATAGAGAGGGCGAAGAAAAAGGGAAGCGAAGAGGATTCCCCAGCATTTTGGGACCCCCAAGAAG ACCAAGCCGCCACGACAACAGTGCAATTGGCAGGGCCATGGAGATACAGAAGTCACGCCACCCGAAGCAC TTATCCACTCCCTCGTCTGTGAGTCCTGAGCCTCAGGACCCTGCCAAGTTGCGCCAGAGTGGAGTAGCCA ATGAAGGAACAGACACTGGATACCTGCCCGCCAGTTCCATGTCCTCTGCCACCTCAGGGACTGCTCTTTC CCAAGAGGGAGGGAGAGAGAATGATACAGGAACAAAACAAGTGGGAGAAGCATCGGCGCCTGGAGACTGC TTGGACAGCACACCTCGAGTCCCCACTACTGTTTTCGACTTCCCTCCTCCTCTATTAGATCAAGTGCAGG AGGAGGAGTGTGAAGTAGAGAGGGTGGCAGAACATGGGACCCCAAAACCCTTTCGGAAGTTTGACAGCAT CGCCTTTGGAGAAAGTCAGAGTGAGGATGAGCAGTTTGAAAATGACCTGGAGACAGATCCACCCAACTGG CAGCAGCTTGTTAGCAGAGAAGTGTTACTGGGACTGAAACCTTCTGAGATCAAAAGACAGGAAGTGATTA ATGAATTGTTCTACACTGAGAGAGCTCATGTCCGGACACTGAAGGTTCTTGACCAAGTATTCTATCAGCG AGTGTCCAGAGAAGGGATTCTATCACCTTCAGAACTACGCAAGATTTTTTCAAACTTGGAAGATATCCTT CAACTTCATGTTGGGTTGAATGAGCAAATGAAAGCTGTCCGAAAAAGGAATGAAACTTCTGTTATTGATC ATATTGGGGAAGATCTGCTCATCTGGTTTAGTGGACCAGGAGAGGAGAAGCTGAAACATGCTGCTGCCAC ATTCTGCAGTAACCAACCTTTCGCCCTGGAAATGATCAAGTCTCGCCAGAAAAAGGATTCTCGATTCCAT ACTTTTGTGCAAGACGCTGAGAGTAACCCCCTGTGCCGTCGTCTTCAGCTGAAGGACATCATTCCCACCC AAATGCAGAGACTCACTAAGTACCCGCTTCTTTTGGATAACATTGCCAAGTACACAGAATGGCCTCCAGA AAGGGAAAAGGTGAAGAAGGCTGCAGACCATTGTCGTCAGATCTTAAATTATGTAAATCAGGCTGTCAGG GAGGCAGAAAACAAGCAGCGCTTAGAAGACTATCAGCGTCGCCTTGACACCTCCAATCTCAAGTTGTCCG AGTACCCAAATGTTGACGAGCTCAGGAATTTGGATTTAACAAAAAGAAAGATGATTCATGAAGGGCCGTT GGTCTGGAAGGTGAATAGAGATAAAAGCATCGATCTTTATACACTGCTGCTGGAAGACATCCTTGTGCTG CTACAGAAGCAGGATGACAGGCTGGTGCTAAGGTGCCACAGCAAGATTCTGGCATCCACAGCCGACAGCA AACACACCTTCAGCCCTGTCATTAAGCTGAGCACGGTGCTGGTTCGGCAGGTGGCGACAGATAACAAAGC TTTATTCGTCATCTCCATGTCAGATAATGGAGCCCAAATTTATGAATTGGTAGCACAGACGGTTTCTGAA AAGACAGTCTGGCAAGACCTCATCTGTCGAATGGCTGCGTCAGTGAAGGAGCAGTCTACAAAGCCGATTC CCCTCCCACAGCCGCCACCTTGCGAAGGAGACAATGATGAAGAAGAACCAGCCAAGTTAAAAGTGGAGCA CCATGATCTTTCAGTCGCTGGTCTGCAAAGTCCAGACAGAGTTTTGGGATTAGAATCTCCCTTAATTTCG TCAAAGCCTCAGTCTCATTCACTGAATACCCCTGGCAAATCAGCGGCAGAACATCTCTTTGTGACCGCAA CACAGTTTGCAAAGGAACAGCATGCAAATGGGGCACTCAAGGAAGGTGATGGCGGGTATCCAGTCACAAT CCCAGGTCCACACTTGCCTGTGTCAGAGGAGCGGTGGGCATTGGACGCACTGAGAAATTTGGGCTTGTTG AAGCAGTTGCTGGTACAGCAGTTAGGTCTCACCGAGAAGAGTACTCAGGAAGACTGGCAGTCCTTCTCAC GATACGGGCCAGCTTCTGAAGAGGTACAGGCAGACAGTGGCATCCGGGACTTGGAAAATGTTAAGGCTTG TCATGCCAGGGAAGGACAGATGTCTTTTAAAACTGGAACTGGTGATATTGCAACTTGTGACAGTCCACGG ACTTCAACTGAGTCTTGTGCTGCACAGGATTCAGTGATACTGGCATCCCAAGACAGCCAGGCAAGTAACG TTTTAGTAATGGACCACATGATCCTGACCCCAGAGATGCCTCCTGCAGAGCCAGAAGGGGGTCTGGATGA GAGTGGAGAGCACTTTTTTGATGCCCGTGAAGCACATAGTGATGATAATCCATCAGAAGGTGATGGAGCA GTTAAAAAGGAAGAGAAGGATGTTAATTTACGCATCTCAGGAAACTGTTTGATCCTTGATGGCTATGATG CAGTTCAGGAGAGCTCCACAGATGAGGAGGTTGCCTCCTCGTTCCCCCTGCAGCCTGTGACGGGCATCCC CGCTGTGGACTCCAGCCACCAGCAGCAACATTCCCCTCAGAATGTGCATCCCGAGGGGCCGGTTTCACCA TTCACCCCAGAATTCCTGGTCCAGCGGCACTGGCGAGCTATGGAGGATACCTGTTTTGAGATCCAGAGTC CCTCATGTACAGATTCACAAAGCCAAATCCTGGAGTACATTCATAAAATAGAGGCTGACCTGGAACACTT AAAGAAGGTGGAGGAAAGTTACGCTCTTCTTTGCCAAAGGCTGGCTGGCTCAGCCCTCCCCGACAAGCTC TCAGATAAAAGTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_027144 |
| Insert Size | 4635 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_027144.2, NP_081420.2 |
| RefSeq Size | 10483 bp |
| RefSeq ORF | 4635 bp |
| Locus ID | 69632 |
| Gene Summary | May play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13). Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase and may act as GTPase-activating protein (GAP) for GNA12 and GNA13 (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG219006 | Arhgef12 (tGFP-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), (10ug) |
CNY 14,920.00 |
|
| MR219006 | Arhgef12 (Myc-DDK-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12) |
CNY 10,568.00 |
|
| MR219006L3 | Lenti ORF clone of Arhgef12 (Myc-DDK-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12) |
CNY 15,490.00 |
|
| MR219006L4 | Lenti ORF clone of Arhgef12 (mGFP-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12) |
CNY 15,490.00 |
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